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SWBZA


Registered: 05/09/23
Posts: 219
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nichrome]
#28672186 - 02/23/24 12:48 AM (4 months, 3 days ago) |
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Quote:
Nichrome said: Those cultures do not look ready to make lc in my eye.
As a matter of interest and for learning: would you take this plate to LC? I'm guessing your comment relates to how well and clean the plate has grown. Correct?
BTW, the little transfer cube has moved off of where it was placed because I flicked the daylights out of the plate. In a different post it was suggested to someone to flick the plate to see whether what they had turns green. The theory was that trich would drop spores causing the color change. I'm full-on here to learn, much more so than just to grow shrooms, so I try weird stuff like that when I read such comments. Needless to say, nothing went green 
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DERRAYLD
Constructus


Registered: 05/13/02
Posts: 10,514
Loc: South Africa
Last seen: 11 hours, 20 minutes
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Re: AGAR ENVY! (Anything and All things agar!) [Re: SWBZA] 2
#28672187 - 02/23/24 12:49 AM (4 months, 3 days ago) |
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That plate you just posted? For lc? Hell no
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SWBZA


Registered: 05/09/23
Posts: 219
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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
#28672208 - 02/23/24 01:43 AM (4 months, 2 days ago) |
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Quote:
DERRAYLD said: That plate you just posted? For lc? Hell no
Okay, more cleaning coming up
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inski
Cortinariologist



Registered: 02/28/06
Posts: 5,778
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Re: AGAR ENVY! (Anything and All things agar!) [Re: SWBZA]
#28672936 - 02/23/24 03:07 PM (4 months, 2 days ago) |
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When you make your next transfer cut a really small wedge from the most uniform looking growth and only from the leading edge of growth.
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Nichrome
dog fart


Registered: 12/17/18
Posts: 7,258
Loc: Zone 5
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Re: AGAR ENVY! (Anything and All things agar!) [Re: SWBZA] 1
#28672993 - 02/23/24 04:16 PM (4 months, 2 days ago) |
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Quote:
SWBZA said:
Quote:
Nichrome said: Those cultures do not look ready to make lc in my eye.
As a matter of interest and for learning: would you take this plate to LC? I'm guessing your comment relates to how well and clean the plate has grown. Correct?
BTW, the little transfer cube has moved off of where it was placed because I flicked the daylights out of the plate. In a different post it was suggested to someone to flick the plate to see whether what they had turns green. The theory was that trich would drop spores causing the color change. I'm full-on here to learn, much more so than just to grow shrooms, so I try weird stuff like that when I read such comments. Needless to say, nothing went green 

No.
If it were me I'd pluck a piece of that sector at 7 o'clock with a pair of fine tweezers and transfer the smallest shred of that growth as you can muster. Also your plate has animal hair or fiber and other dust/debris on it. This is indicative of mistreatment in general and you need to refine your cleanliness to be able to preform sterile procedures properly. You are preforming neurosurgery on babies. They are strong babies that have the potential to live forever but babies none the less.
It all needs work but you are getting there and will be a wizard in no time. The sectoring is a good sign you will be able to get cohesive colonies out of that plate eventually and the strength of 7 o'clock there is promising.
-------------------- question everything, it's very responsive “Drink your tea slowly and reverently, as if it is the axis on which the world earth revolves - slowly, evenly, without rushing toward the future.” ― Thich Nhat Hanh Gypsum
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SWBZA


Registered: 05/09/23
Posts: 219
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nichrome]
#28673552 - 02/24/24 01:14 AM (4 months, 1 day ago) |
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Inski, Nichrome, thanks for the feedback. Tweezing done
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Nichrome
dog fart


Registered: 12/17/18
Posts: 7,258
Loc: Zone 5
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Re: AGAR ENVY! (Anything and All things agar!) [Re: SWBZA]
#28673556 - 02/24/24 01:19 AM (4 months, 1 day ago) |
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-------------------- question everything, it's very responsive “Drink your tea slowly and reverently, as if it is the axis on which the world earth revolves - slowly, evenly, without rushing toward the future.” ― Thich Nhat Hanh Gypsum
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Pnin
Riz Gukgak


Registered: 07/18/23
Posts: 494
Last seen: 13 hours, 7 minutes
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nichrome]
#28674323 - 02/24/24 03:07 PM (4 months, 1 day ago) |
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Anyone wanna give some input on these? I have kept transferring to try to alter the growth presentation on agar and it's been consistent since t2. Looks like mold to me but grows the pace of myc, or even a bit slower. Country cock and phobos.


For comparison, a plate I'd consider healthy.
-------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿 >my end of 2023 grow journal<
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Nichrome
dog fart


Registered: 12/17/18
Posts: 7,258
Loc: Zone 5
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Pnin] 2
#28674339 - 02/24/24 03:22 PM (4 months, 1 day ago) |
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They all look "healthy" and axenic, but young and they don't know who they are yet. They don't have much life experience to draw from. Be patient, and show them you are being patient. They learn an immense amount and rapidly compared to us. Their genius and capability dwarves our own ability and understanding.
You can watch the areal mycelium in a dish vibrate from sonic energy from your voice under a microscope. They are very sensitive, have the ability to essentially live forever and grow to be massive diverse colonies. They are in no rush and those plates are baby godlike beings. It may take them time to figure it out just like the human race in the current world. Babies fighting amongst themselves, and also people trying to wait out that process. People with some magical memory of what happens next and why all this is worth it. People with a built in design for a healthy productive future and harmoniousness.
If you get bored of waiting for maturity to develop in potentially immortal children, you can make an lc, store it in a mature state in a vial in the fridge for a few years, then bring it out and plate it up. You'll see a break in the pattern and all sorts of new patterns may emerge and form cohesive sectors and patterns. They have time to get to know each other as lc. It is a good environment for them to come to terms and develop purpose.
-------------------- question everything, it's very responsive “Drink your tea slowly and reverently, as if it is the axis on which the world earth revolves - slowly, evenly, without rushing toward the future.” ― Thich Nhat Hanh Gypsum
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LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 2,034
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nichrome]
#28674388 - 02/24/24 03:57 PM (4 months, 1 day ago) |
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I'd also consider those as mold
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Nichrome
dog fart


Registered: 12/17/18
Posts: 7,258
Loc: Zone 5
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Re: AGAR ENVY! (Anything and All things agar!) [Re: LadysKnight] 2
#28674396 - 02/24/24 04:04 PM (4 months, 1 day ago) |
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But you can see the clamps practically from outer space. 
Mold doesn't do that.
-------------------- question everything, it's very responsive “Drink your tea slowly and reverently, as if it is the axis on which the world earth revolves - slowly, evenly, without rushing toward the future.” ― Thich Nhat Hanh Gypsum
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Lemgrub



Registered: 04/02/18
Posts: 644
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nichrome] 1
#28674567 - 02/24/24 06:27 PM (4 months, 1 day ago) |
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Not mold, just haven't found the right subculture.
-------------------- We getting that pirate bay, alien shishkebab cordycep money

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chris77
Archaic Revivalist



Registered: 01/17/22
Posts: 1,996
Last seen: 12 hours, 6 minutes
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Lemgrub] 1
#28674999 - 02/25/24 01:13 AM (4 months, 23 hours ago) |
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team no mould
@nichrome:
-------------------- the observer is the observed j. krishnamurti
Edited by chris77 (02/25/24 01:13 AM)
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 2,256
Loc: USA
Last seen: 3 hours, 34 minutes
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Re: AGAR ENVY! (Anything and All things agar!) [Re: chris77]
#28677283 - 02/26/24 05:55 PM (3 months, 30 days ago) |
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Ok I have a dandy. I have done probably a hundred T1s and this is a first for me!



And the parent dish

Food for thought 🧐 smells like terrific mycelium.
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ReverendMyc
succinct is not my forte

Registered: 03/29/19
Posts: 2,022
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Re: AGAR ENVY! (Anything and All things agar!) [Re: SupaThaRipper]
#28677349 - 02/26/24 06:50 PM (3 months, 30 days ago) |
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The search team term that you are looking for is Scalloped or scalloping. Stipe-n-cap has done some work but I don't think he has come up with any answer other than that they produce poorly if at all.
I just started a test to see if I can clean up a scalloped tampanensis culture that I want to preserve using a brf puck. But just started that a couple of days ago, so nothing to report yet.
Quote:
ReverendMyc said: ... Trying something else now. I have a tamanensis culture that I really want to preserve. They have proven tricky because the spore prints seem to be unrecoverable after about a year to 18 months for me with tamps. I did manage to get one of my old prints to germ, but several transfers in and I am still getting scalloping plates. I have seen a few theories on scalloped edges and enmeshed contam is a common one.
So lets see if using a puck will help.
  1469 was the successful germ, 1507 is t1, 1538 & 1537 are t2, 1593 & 1592 are t3.
  I took two transfers from 1592 to the top two pucks and 2 more to plates to see how things progress. I followed a suggestion from Baba Yaga to place the puck samples on one side of the pucks and will transfer from the other side when the mycelium reaches that far. And hey, if the plates come out clean too, I won't even be mad. ...
https://www.shroomery.org/forums/showflat.php/Number/28676781#28676781
-------------------- Stoned Gummys | BRF Pucks | Primo Prepour Plates | Easy LI 4 Preserv & Propo"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023 Don't Panic   
Edited by ReverendMyc (02/26/24 06:56 PM)
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 2,256
Loc: USA
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Re: AGAR ENVY! (Anything and All things agar!) [Re: ReverendMyc] 1
#28677358 - 02/26/24 07:00 PM (3 months, 30 days ago) |
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Thanks rev! I’ve seen it but never to such a degree before. I do think they look beautiful, like flowers 😂 hopefully the brf works! Already love brf pucks so much so that would be another great perk!
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LemonHead
It's a Lemon


Registered: 02/08/24
Posts: 54
Last seen: 5 days, 2 hours
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
#28677695 - 02/27/24 12:50 AM (3 months, 30 days ago) |
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Hi
I'm back and I'm curious about a few things These are all Pink Oyster
 In the top middle there's these dots that are forming. Is that the mycelium coming together? It looks cool
 Here's another example but directly in the middle there's a new dot forming
 With this too I had squirted 3 drops of LC near the center of the plate but these dots had been popping up allover. Is it contam or just mycelium?
 This one I had left in here a while after sectioning off some pieces There's this discoloration popping up in some areas. I vaguely remember that Pink Oyster Mycelium can turn pinkish is that what this is? To the eye it does kind of look pinkish but that could just be my brain playing tricks on me
Either way nothing urgent (i hope) just curiosity
Thank you for your time
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pablotx
pipette monkey


Registered: 05/06/23
Posts: 78
Loc: Hot & humid
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Re: AGAR ENVY! (Anything and All things agar!) [Re: LemonHead]
#28677839 - 02/27/24 07:48 AM (3 months, 29 days ago) |
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Golden Teacher T2s, transferred on 2/11. Do they look ok?
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 2,256
Loc: USA
Last seen: 3 hours, 34 minutes
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Re: AGAR ENVY! (Anything and All things agar!) [Re: pablotx]
#28677848 - 02/27/24 07:52 AM (3 months, 29 days ago) |
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They look good but the right one looks gooder. I do one more from the right personally, but I’m never in a rush to get anything on spawn
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pablotx
pipette monkey


Registered: 05/06/23
Posts: 78
Loc: Hot & humid
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Re: AGAR ENVY! (Anything and All things agar!) [Re: SupaThaRipper]
#28677900 - 02/27/24 08:41 AM (3 months, 29 days ago) |
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I'm good with gooder. I'll make a couple more transfers from the right one... thanks!
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