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Offlinekoma23
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Registered: 09/15/20
Posts: 117
Last seen: 1 day, 12 hours
Re: AGAR ENVY! (Anything and All things agar!) [Re: Mr Piggy]
    #28670770 - 02/22/24 02:48 AM (4 months, 3 days ago)

Quote:

Mr Piggy said:
Quote:

DERRAYLD said:
You shouldn't if your culture is clean and ready for grain,then you can cut up the entire plate.




Given that it was a t1 with a slightly questionable middle, I would absolutely transfer from the edge.




Yeah, I followed exactly that logic yes. Tnx, appreciate the help!

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OfflineSupaThaRipper
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Registered: 09/02/13
Posts: 2,256
Loc: USA
Last seen: 3 hours, 34 minutes
Re: AGAR ENVY! (Anything and All things agar!) [Re: LemonHead] * 2
    #28670816 - 02/22/24 04:40 AM (4 months, 3 days ago)

You definitely don’t want to go 10 days without looking at your plates. Especially a clone plate (if you’re new annd your technique has some work to do) and germination plates. Check every day! You want to take transfer(s) as soon as you see clean growth. Other contaminations (if they do pop up) can quickly override your clean mycelium. Mycelium being stunted by bringing a petri into the light is the most ridiculous thing I’ve heard. I’ve always left all my petris out in the light.

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Offlinefiddle_head
sporophyte
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Registered: 08/05/08
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #28670819 - 02/22/24 04:43 AM (4 months, 3 days ago)

sorry to double post, anyone know what were looking at here?



--------------------
lagm 2024 || lagm 2023 || 2023 || Things That Are Good
=============
If you cannot afford petris you can still do this hobby.
🌿 Looking for spores? Look no further. πŸ’

XI πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ XI

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OfflineLemonHead
It's a Lemon
I'm a teapot Unread Journal

Registered: 02/08/24
Posts: 54
Last seen: 5 days, 2 hours
Re: AGAR ENVY! (Anything and All things agar!) [Re: SupaThaRipper]
    #28670824 - 02/22/24 04:49 AM (4 months, 3 days ago)

That makes sense yeah
It's my first time so I have no idea what's right and what's wrong. I read that you can just leave them out to incubate, but again I don't know for sure what is right or not

Since I don't know if I'm handling them properly yet I would imagine being able to check up on them frequently would help

I guess I'll figure out what works and what doesnt one way or another lol

Thank you very much for your help

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Invisiblearnold582

Registered: 10/30/23
Posts: 89
Loc: Mars
Re: AGAR ENVY! (Anything and All things agar!) [Re: LemonHead]
    #28670858 - 02/22/24 05:28 AM (4 months, 3 days ago)

Is this mold next to the mycelium? I've been waiting to see if it's going to change colors, but it's been a week and it's still white.




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Offlinefiddle_head
sporophyte
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #28670894 - 02/22/24 06:07 AM (4 months, 3 days ago)

cant tell from the pics but something is up. can you get a clearer shot? your lens is focusing on the top of the plate. shine alight thru the side and tap to focus beneath the lid. if you can.


--------------------
lagm 2024 || lagm 2023 || 2023 || Things That Are Good
=============
If you cannot afford petris you can still do this hobby.
🌿 Looking for spores? Look no further. πŸ’

XI πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ XI

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Invisiblearnold582

Registered: 10/30/23
Posts: 89
Loc: Mars
Re: AGAR ENVY! (Anything and All things agar!) [Re: fiddle_head]
    #28670908 - 02/22/24 06:20 AM (4 months, 3 days ago)

Quote:

fiddle_head said:
cant tell from the pics but something is up. can you get a clearer shot? your lens is focusing on the top of the plate. shine alight thru the side and tap to focus beneath the lid. if you can.




Sorry, it's a challenge with all the condensation. This should be better.




Edited by arnold582 (02/22/24 06:24 AM)

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Offlinefiddle_head
sporophyte
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 1
    #28670919 - 02/22/24 06:32 AM (4 months, 3 days ago)

im still not sure, but i wouldnt use it. i in fact actively advise you not to send that plate to grain. perform a transfer to obtain clean culture, then send.


--------------------
lagm 2024 || lagm 2023 || 2023 || Things That Are Good
=============
If you cannot afford petris you can still do this hobby.
🌿 Looking for spores? Look no further. πŸ’

XI πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ XI

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OfflineDERRAYLD
Constructus
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Registered: 05/13/02
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Re: AGAR ENVY! (Anything and All things agar!) [Re: fiddle_head]
    #28670925 - 02/22/24 06:39 AM (4 months, 3 days ago)

If it's not clean and uniform keep transferring

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InvisibleSWBZA
Male

Registered: 05/09/23
Posts: 219
Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #28670949 - 02/22/24 07:15 AM (4 months, 3 days ago)

Does this look normal for a germ plate? Scraped a spore print with inoculation loop, scraped over the agar plate. You can see the two transfers I made as well. I would expect much contamination. Maybe it is there and I just can't see it. My problems are usually around bacteria, not mold plates.


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OfflineSmellyhobbitM
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Registered: 04/01/22
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Re: AGAR ENVY! (Anything and All things agar!) [Re: SWBZA]
    #28670952 - 02/22/24 07:17 AM (4 months, 3 days ago)

Looks pretty normal to me. Most of my germ plates are actually pretty dang clean.


--------------------
A Love Letter to New Growers
A Guide for New Growers
Need Spores? - Sablabs.org

Just because your tub contamed, doesn’t mean your attitude has to contam as well. :mushroom:


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InvisibleSWBZA
Male

Registered: 05/09/23
Posts: 219
Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit]
    #28671005 - 02/22/24 08:13 AM (4 months, 3 days ago)

That is good news! Thanks.

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InvisibleReverendMyc
succinct is not my forte

Registered: 03/29/19
Posts: 2,022
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Re: AGAR ENVY! (Anything and All things agar!) [Re: LemonHead] * 2
    #28671211 - 02/22/24 10:45 AM (4 months, 3 days ago)

Quote:

LemonHead said:
...
It's my first time so I have no idea what's right and what's wrong. I read that you can just leave them out to incubate, but again I don't know for sure what is right or not
Since I don't know if I'm handling them properly yet I would imagine being able to check up on them frequently would help
I guess I'll figure out what works and what doesnt one way or another lol
...



Supa is leading you right.

I am not trying to run anyone down who is trying to help, however, I like to look at post count and time since registering or for a TC (trusted cultivator) tag when seeking advice. There are some experienced folks who are new on this board and give great advice, but for the most part when confronted with conflicting information, look to the documented experience on this board for solid answers.

Good luck!


--------------------
Stoned Gummys | BRF Pucks | Primo Prepour Plates | Easy LI 4 Preserv & Propo
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic



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OfflineKnut
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Registered: 12/28/23
Posts: 1
Last seen: 4 months, 2 days
Re: AGAR ENVY! (Anything and All things agar!) [Re: ReverendMyc] * 1
    #28671367 - 02/22/24 12:56 PM (4 months, 3 days ago)

Hello everyone,
Our first post here on the shroomery.
2 years ago we started growing mushroom doing the pf-tek during the covid-lockdown using the shroomery. Recently we have started using agar-plates with the goal of doing a grainspawn in a monotub. Thank you for all for all the knowledge we alread received from you, here is a picture of one of our first agar plates!
It is brown because we couldn't find light malt extract.

Greetings,
Knut

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Offlinetholos
Stranger
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Registered: 03/06/23
Posts: 564
Last seen: 4 days, 6 hours
Re: AGAR ENVY! (Anything and All things agar!) [Re: Knut] * 3
    #28671549 - 02/22/24 03:04 PM (4 months, 3 days ago)



Yeti

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Offlinemycoheadonaut
Stranger
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Registered: 12/26/23
Posts: 86
Last seen: 24 days, 2 hours
Re: AGAR ENVY! (Anything and All things agar!) [Re: ReverendMyc]
    #28671580 - 02/22/24 03:30 PM (4 months, 3 days ago)

Quote:

ReverendMyc said:
Quote:

LemonHead said:
...
It's my first time so I have no idea what's right and what's wrong. I read that you can just leave them out to incubate, but again I don't know for sure what is right or not
Since I don't know if I'm handling them properly yet I would imagine being able to check up on them frequently would help
I guess I'll figure out what works and what doesnt one way or another lol
...



Supa is leading you right.

I am not trying to run anyone down who is trying to help, however, I like to look at post count and time since registering or for a TC (trusted cultivator) tag when seeking advice. There are some experienced folks who are new on this board and give great advice, but for the most part when confronted with conflicting information, look to the documented experience on this board for solid answers.

Good luck!





Good job brother when I first started growing 10 years ago misinformation was a huge problem I can see it's getting better πŸ‘

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OfflineLemonHead
It's a Lemon
I'm a teapot Unread Journal

Registered: 02/08/24
Posts: 54
Last seen: 5 days, 2 hours
Re: AGAR ENVY! (Anything and All things agar!) [Re: ReverendMyc] * 1
    #28671792 - 02/22/24 06:33 PM (4 months, 3 days ago)

Yeah I'm in no position to really disregard anything yet until I learn what works and what doesn't so thank you for clearing things up

I'll look for that TC tag

Thank you all for your help it means a lot to me
Hopefully we can get these boys to grow

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InvisibleSWBZA
Male

Registered: 05/09/23
Posts: 219
Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #28672141 - 02/22/24 11:20 PM (4 months, 3 days ago)

Hi Team Shroomery.

I want to try Josex's Poke to make an LC.  Do I use the normal LME agar plate or the water agar plate as donor?



Why? 

Any suggestions on where off the plate(s) to take the poke?

Kind regards and thanks in advance

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InvisibleNichrome
dog fart
I'm a teapot User Gallery

Registered: 12/17/18
Posts: 7,258
Loc: Zone 5
Trusted Cultivator
Re: AGAR ENVY! (Anything and All things agar!) [Re: SWBZA] * 1
    #28672144 - 02/22/24 11:26 PM (4 months, 3 days ago)

A clean piece is a clean piece. It doesn't matter what the starting media is if it is going to nutrient broth. Whatever is convenient for you to do is the right move.

Those cultures do not look ready to make lc in my eye.


--------------------
question everything, it's very responsive



β€œDrink your tea slowly and reverently, as if it is the axis on which the world earth revolves - slowly, evenly, without rushing toward the future.”
― Thich Nhat Hanh


Gypsum

Edited by Nichrome (02/22/24 11:27 PM)

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InvisibleSWBZA
Male

Registered: 05/09/23
Posts: 219
Re: AGAR ENVY! (Anything and All things agar!) [Re: Nichrome]
    #28672162 - 02/23/24 12:02 AM (4 months, 3 days ago)

Quote:

Nichrome said:
A clean piece is a clean piece. It doesn't matter what the starting media is if it is going to nutrient broth. Whatever is convenient for you to do is the right move.

Those cultures do not look ready to make lc in my eye.



Thanks for that. I'll let them run longer and see what happens.

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Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Bulk Substrate   OlympusMyco.com Olympus Myco Bulk Substrate   Mushroom-Hut Substrate Bags   MagicBag.co All-In-One Bags That Don't Suck   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract


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