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Offlineballysocket
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Re: pe on agar [Re: HelloImBob] * 1
    #28630693 - 01/22/24 06:23 AM (5 months, 2 days ago)

Quote:

HelloImBob said:
Plus what does it matter to make a few new plates, a little time and effort but it will be worth it. A few drops of spores?

Your not trying to fruit on your agar and nutrition is way overboard either way... You can get amazing results with just grain soak water LC/agar.

Nobody is trying to be mean they are just speaking from experience, it's harder to tell on that kind of background what your looking at compared to like looking at jello...



Thank you.
It's 8% grain flour, as prescribed in the article I read. This mix worked really well for cloning. Would food coloring work for visual identification or does it need to be translucent?

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Offlineballysocket
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Re: pe on agar [Re: HappinessStan]
    #28630700 - 01/22/24 06:28 AM (5 months, 2 days ago)

Quote:

HappinessStan said:
Quote:

ballysocket said:
I have three plates that I inoculated with a pe mss. My only agar experience is cloning pins from a pf tek. Does any of this mess look viable?
Thank you in advance!







I think you need to start again with better advice.
That is almost certainly cube mycelium but, it's completely shrouded by obvious contams.
How did you sterilise those agar plates?



I pc'd for 45 minutes. One of the reasons I chose grain flour was to pc a bit longer without worrying about overcooking sugar. Also, I thought it would be beneficial to germinate on the food source I will be colonizing for spawn.
Maybe I just stick to cloning pins.
Thank you.

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Offlineballysocket
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Re: pe on agar [Re: ballysocket]
    #28668369 - 02/20/24 03:32 PM (4 months, 4 days ago)

So I put my vomit flavored agar in the fridge and forgot about it for a while. I finally broke down and ordered some lme. I made a transfer to this boring ass shit. What am I looking at?

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InvisibleOctopusDisco
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Re: pe on agar [Re: ballysocket] * 1
    #28668478 - 02/20/24 04:24 PM (4 months, 4 days ago)

Quote:

ballysocket said:
So I put my vomit flavored agar in the fridge and forgot about it for a while.
...




So it both looked and tasted like vomit?!

I kid, I kid :smile:

The boring agar makes it MUCH easier to interpret a well-focused picture. Based on what I can see there--and leveraging my own limited experience--I'd say things are looking good and what I'd expect from MSS!

Out of curiosity, what kind of camera are you using?

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Offlineballysocket
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Re: pe on agar [Re: OctopusDisco]
    #28668541 - 02/20/24 05:10 PM (4 months, 4 days ago)

It's a very cheap cell phone. Moto g power. Are the pictures that bad?
Anyway I'm guessing it needs to grow out a bit more before picking my spot for the next transfer?

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InvisibleOctopusDisco
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Re: pe on agar [Re: ballysocket]
    #28669241 - 02/21/24 07:22 AM (4 months, 3 days ago)

The reason I ask about the camera is that some digital cameras (and I think some cell phones too) allow the user to change the focus depth. In the picture in the last post, the camera is auto-focusing on the top of the lid instead of on the culture.

The new agar recipe makes it much easier to tell what's going on, even if it's a bit out of focus!

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Invisiblemeta_mmxxii
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Re: pe on agar [Re: OctopusDisco]
    #28669801 - 02/21/24 01:50 PM (4 months, 3 days ago)

OP I think you are mixing up a couple of teks there, when one makes agar from grain water runoff, the grain water acts as the nutrients for the mycelium, you should not have to add anything other than water and agar-agar powder, and food coloring if you want it colored.
Adding crushed up grain to it does nothing but make your agar look like "puke" as you describe it, and I actually agree with that assessment. Malt Extract Agar (MEA) and Potato Dextrose Agar (PDA) is the most common used recipes because they are proven to work, and reliable for culture work. You want as clear an agar as you can get so you can clearly see any contaminations in your samples.
You have received some pretty solid advice in making new agar correctly and have a much better chance at success.
I have learned when one steps out of convention, it is rarely a successful endeavor as it more than likely has been tried and is found to be unreliable, and it makes it harder for others to advise some one that does.


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Offlineballysocket
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Re: pe on agar [Re: meta_mmxxii]
    #28670038 - 02/21/24 04:47 PM (4 months, 3 days ago)

Thank you. With that being said, does the new dish look viable? Should I make another transfer? Also, is it worthwhile trying to isolate from spore or better to clone proven growers?
Thanks again.

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Offlinesmalltalk_canceled
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Re: pe on agar [Re: ballysocket] * 2
    #28670053 - 02/21/24 05:04 PM (4 months, 3 days ago)

Quote:

ballysocket said:
Quote:

HappinessStan said:
Quote:

ballysocket said:
I have three plates that I inoculated with a pe mss. My only agar experience is cloning pins from a pf tek. Does any of this mess look viable?
Thank you in advance!







I think you need to start again with better advice.
That is almost certainly cube mycelium but, it's completely shrouded by obvious contams.
How did you sterilise those agar plates?



I pc'd for 45 minutes. One of the reasons I chose grain flour was to pc a bit longer without worrying about overcooking sugar. Also, I thought it would be beneficial to germinate on the food source I will be colonizing for spawn.
Maybe I just stick to cloning pins.
Thank you.








Reach for heaven



Abandon no pour

Join Petri dish alliance



Also honestly, they are easier to work with than no pours with depth.

Im finally out of the closet - petris are just better


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Offlineballysocket
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Re: pe on agar [Re: meta_mmxxii] * 1
    #28670055 - 02/21/24 05:06 PM (4 months, 3 days ago)

Not to keep beating a dead horse, but this is an excerpt from Psilicon's agar write up, which is referenced/linked in the Hitchhiker's Guide. Am I misinterpreting?

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InvisibleOctopusDisco
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Re: pe on agar [Re: ballysocket]
    #28670075 - 02/21/24 05:26 PM (4 months, 3 days ago)

Does the new dish look viable?: Again, I'm relatively new at this hobby, but I don't see any obvious signs of contamination (the picture is a bit blurry, so there might be some weird stuff that I can't clearly see, however).

Should you make another transfer: If it were me, I'd let that plate grow out. I think you're in the clear vis a vis bacterial infection based on the absence of goo puddles. Next thing to check for is mold contamination. If the culture stays white, I'd say you're in the clear there too. I've heard of some mold cultures that become enmeshed and are pretty nasty because they won't sporulate on plates. If that's the case, then you need a microscope to properly identify and some advanced techniques to clean the culture. Unless you're prepared to go down that rabbit hole, I'd check to make sure the culture stays white and assume it's mold-free. Worst-case scenario, you lose a tub instead of another month trying to clean a super-dirty culture.

Is it worthwhile trying to isolate from spore or better to clone proven growers?: From what I've seen, this is a question that does not have a definitive answer. I've heard of cultivators "isolating" strains on agar by taking transfers from different sectors, ostensibly creating something close to a monoculture. I've also heard cultivators arguing that cloning a fruit will produce a culture with many genetic strains, while others argue it produces a monoculture. My advice would be to send a clean plate to grain and clone the winners!

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InvisibleOctopusDisco
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Re: pe on agar [Re: ballysocket]
    #28670089 - 02/21/24 05:35 PM (4 months, 3 days ago)

Quote:

ballysocket said:
Not to keep beating a dead horse, but this is an excerpt from Psilicon's agar write up, which is referenced/linked in the Hitchhiker's Guide. Am I misinterpreting?
[/url]




I think you followed the instructions correctly, and nowhere do the instructions say to filter or strain out the solids (I CTRL+F-ed "filter", "strain", "solid", and "sediment" and there is nothing there).

The instructions should say to filter or strain out the solids because clarity is important.

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Offlineballysocket
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Re: pe on agar [Re: OctopusDisco]
    #28670349 - 02/21/24 08:00 PM (4 months, 3 days ago)

Thanks. I appreciate all the input. :thumbup:

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Offlineballysocket
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Re: pe on agar [Re: meta_mmxxii] * 1
    #28670385 - 02/21/24 08:15 PM (4 months, 3 days ago)

Quote:

meta_mmxxii said:
OP I think you are mixing up a couple of teks there, when one makes agar from grain water runoff, the grain water acts as the nutrients for the mycelium, you should not have to add anything other than water and agar-agar powder, and food coloring if you want it colored.
Adding crushed up grain to it does nothing but make your agar look like "puke" as you describe it, and I actually agree with that assessment. Malt Extract Agar (MEA) and Potato Dextrose Agar (PDA) is the most common used recipes because they are proven to work, and reliable for culture work. You want as clear an agar as you can get so you can clearly see any contaminations in your samples.
You have received some pretty solid advice in making new agar correctly and have a much better chance at success.
I have learned when one steps out of convention, it is rarely a successful endeavor as it more than likely has been tried and is found to be unreliable, and it makes it harder for others to advise some one that does.



I see you have a link to the Hitchhiker's Guide in your signature. Have you yourself read it and the attached write-ups? You and others are criticizing me for "stepping out of convention" while in reality I was following instructions to the letter.
There's a post that keeps popping up in the recent pages; something titled Can The Shroomery Be Saved or something like that. I'm leaning towards nope on that one. Not with the intolerant contradictory douchebaggery from self proclaimed experts trying to profess yet hadn't read the text. It would be cooler if you know-it-alls would take the time to actually answer the questions or simply don't.

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Onlinefiddle_head
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Re: pe on agar [Re: ballysocket] * 1
    #28670404 - 02/21/24 08:27 PM (4 months, 3 days ago)

no reason to get upset bud everyones just trying to help you. the internet can be a confusing place for communication, emotion.

you are fine to take transfers from that plate, the advantage to letting it grow out would be to get a better idea of which leading edges to take from. either way, its an acceptable donor plate and does not display contamination visually.

pda works miracles for me. just put some in a french press or steep it in cold water somehow. strain it and put it in your media vessel with agar. no need to strain lme. you dont even have to strain anything if you dont want, but it helps me. like it was said earlier, some media is just inherently cloudy and low-visibility.

what i try to do is find the best performing cultures by growing them out. its the only way to see how they perform. good appearance or performance on agar does not guarantee anything down the line and mycelium changes appearance on different media esp when it is transferred to grain.

if you cant get good canopies, go back to spore and try to isolate a different culture. if it does good, select a fruit with the desired traits. clone, grow out, collect spore, grow out, clone, etc ad infinitum.


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Offlineballysocket
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Re: pe on agar [Re: fiddle_head] * 1
    #28670874 - 02/22/24 05:47 AM (4 months, 2 days ago)

Thank you for your input. It clears up my questions.
But I don't believe everyone is trying to help. There are some highly visible contributes here that clearly have no intention of addressing the questions asked. To me it seems they are just bored and lonely and are desperate to be a part of a conversation for better or worse. What percentage of posts from new learners are predicated with some sort of self deprecation such as "sorry I'm a noob" or anything like that. This is clearly because they have read a LOT of threads with these highly visible posters being overly critical for their own purpose, and dread what they are about to subject themselves to by asking a question.

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Onlinefiddle_head
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Re: pe on agar [Re: ballysocket]
    #28670879 - 02/22/24 05:54 AM (4 months, 2 days ago)

Quote:

ballysocket said:
Thank you for your input. It clears up my questions.
But I don't believe everyone is trying to help. There are some highly visible contributes here that clearly have no intention of addressing the questions asked. To me it seems they are just bored and lonely and are desperate to be a part of a conversation for better or worse. What percentage of posts from new learners are predicated with some sort of self deprecation such as "sorry I'm a noob" or anything like that. This is clearly because they have read a LOT of threads with these highly visible posters being overly critical for their own purpose, and dread what they are about to subject themselves to by asking a question.




welcome


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If you cannot afford petris you can still do this hobby.
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OfflineDERRAYLD
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Re: pe on agar [Re: ballysocket] * 1
    #28670881 - 02/22/24 05:55 AM (4 months, 2 days ago)

Stop acting like a victim, everyone that contributes here does so freely and makes every effort to assist whenever possible.

There are times when many will stop responding because the information is freely available on this site if you just search for it with the effective search function restricted to the last 5 years.

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Onlinefiddle_head
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Re: pe on agar [Re: ballysocket] * 2
    #28670890 - 02/22/24 06:03 AM (4 months, 2 days ago)

We will teach you how to grow the fuck out of some shrooms but we dont cater to emotion or delicate sensibilities here as it is the internet, either way you are right, itΒ΄s not for everyone. just wanted to say that. i hope you stick around and come around ya knoe.


--------------------
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=============
If you cannot afford petris you can still do this hobby.
🌿 Looking for spores? Look no further. πŸ’

XI πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ XI

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Offlineballysocket
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Re: pe on agar [Re: fiddle_head] * 1
    #28670977 - 02/22/24 07:50 AM (4 months, 2 days ago)

This thread has obviously become an annoyance. If anyone knows how to close it out please do. I'm not feeling like a victim, but it is frustrating to be told black is white, up is down. Id invite you to read through. How much of the input was irrelevant to the question and/or factually inaccurate with regards to the recommended reading for amateur growers? I have always been appreciative of informative input and hopefully will continue to; for giggles I threw a few chunks of vomagar into some practice bags of oats (first time working with oats). I'm intrigued by their appearance. I'll post  pictures in a different thread at some point.

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