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fnulnu
Student



Registered: 12/20/23
Posts: 358
Loc: terrapin station, in the shado...
Last seen: 6 hours, 48 minutes
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Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD]
#28668047 - 02/20/24 11:25 AM (4 months, 4 days ago) |
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Quote:
DERRAYLD said: Bottom water by pouring some water down the sides and waiting for it to absorb, add more if needed but don't saturate the substrate.
It is a natural part of the process, as the mushrooms use the water in the substrate it shrinks.
Thank you! I did some spraying down the sides and I will go back and maybe add more.
-------------------- https://www.shroomery.org/forums/showflat.php/Number/28645465 A noob that would sure love some constructive criticism ..I have a learning difficulty, but I think I got this!
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,438
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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy] 1
#28668052 - 02/20/24 11:26 AM (4 months, 4 days ago) |
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Quote:
metalface said:
 
What's this sandy thing in my popcorn jar? Definitely a toss, just curious what it could be. It showed up after first break and shake at 20%.
Quote:
Mr Piggy said: Sorry homie, that's a contam. looks like yeast.
Correct, that is yeast.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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fnulnu
Student



Registered: 12/20/23
Posts: 358
Loc: terrapin station, in the shado...
Last seen: 6 hours, 48 minutes
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Re: Ask Quick Questions, Get Quick Answers [Re: elasticaltiger] 1
#28668063 - 02/20/24 11:34 AM (4 months, 4 days ago) |
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Some anonymous person on the street corner stopped me and asked:
"Is it okay to cut out visual contam from the jars and spawn to coir? It looks like 2/6 jars have a small contam...can throw it away if that's what is needed, don't want to contaminate other totes etc"
Just curious
-------------------- https://www.shroomery.org/forums/showflat.php/Number/28645465 A noob that would sure love some constructive criticism ..I have a learning difficulty, but I think I got this!
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GenesisCorrupted
Taoist, Writer, Student, Artist




Registered: 08/01/23
Posts: 12,928
Loc: PNW
Last seen: 27 minutes, 18 seconds
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Re: Ask Quick Questions, Get Quick Answers [Re: fnulnu] 1
#28668064 - 02/20/24 11:36 AM (4 months, 4 days ago) |
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It’s basically a lost cause. It could have contamination anywhere you’re just able to see some of it. The jar is dead. The most you could do and I was able to do this. Take a chunk out of the jar that looks like the most viable bit of mycelium. And then you can transfer that to agar and clean it up.
At least then, the spore can be salvaged.
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fnulnu
Student



Registered: 12/20/23
Posts: 358
Loc: terrapin station, in the shado...
Last seen: 6 hours, 48 minutes
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Thank you!
I still have some mycellium on agar, and other jars going.....I was just excited to start a mono with 6 jars full!
-------------------- https://www.shroomery.org/forums/showflat.php/Number/28645465 A noob that would sure love some constructive criticism ..I have a learning difficulty, but I think I got this!
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SonomaFungi_707
Stranger
Registered: 12/24/23
Posts: 229
Last seen: 22 days, 2 hours
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Re: Ask Quick Questions, Get Quick Answers [Re: fnulnu]
#28668133 - 02/20/24 12:47 PM (4 months, 4 days ago) |
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Quote:
fnulnu said: Thank you!
I still have some mycellium on agar, and other jars going.....I was just excited to start a mono with 6 jars full!
You
Quote:
fnulnu said: Thank you!
I still have some mycellium on agar, and other jars going.....I was just excited to start a mono with 6 jars full!
You could still do four shoeboxes?
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tossed_salad
Rad Dude



Registered: 05/02/23
Posts: 88
Loc: USA
Last seen: 1 day, 22 hours
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So clearly I have knotting and a few tiny pins it looks like. Is there anything I should do I this point?
I'm very excited and don't want to screw up my first successful grow
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AspectOfTheCreator
Mastering the Art of Success



Registered: 12/07/22
Posts: 1,786
Last seen: 25 minutes, 37 seconds
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Re: Ask Quick Questions, Get Quick Answers [Re: tossed_salad] 1
#28668470 - 02/20/24 04:19 PM (4 months, 4 days ago) |
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Leave it alone
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DarkStarPsychonaut
Dr.



Registered: 10/03/20
Posts: 278
Loc: Terrapin station, space
Last seen: 3 days, 6 hours
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I’ve got a shoebox and a sweater box of starry night APEs. They are looking pretty blobby on this first flush , while I had some other first flushes from the same culture that were great. I am thinking about harvesting tomorrow and hoping for a better shaped flush 2. I was out of town for the past 5-6 days so I haven’t been able to watch the blobs to see if they are still growing in the past few days. Does it make sense to harvest as is or should I observe for a day or two and see if any growth is still happening? Anything I can do between flushes to reduce chance of blobs?
-------------------- Noob level III here, thanks to those currently active here and those that went before for all the help!
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Skropi
Space Ritual


Registered: 11/01/23
Posts: 414
Loc: Greece
Last seen: 1 month, 19 days
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Never grew APEs, but from what I've read, it is usually the second flush that blobs out. I would expect the second flush to be different. Then again, I am a known optimist.
edit. Still not sure if I am right, but I meant to say that it is the first flush. Damn, I should double check my posts.
-------------------- Ἄνδρα μοι ἔννεπε, Μοῦσα, πολύτροπον
Edited by Skropi (02/21/24 01:14 PM)
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DarkStarPsychonaut
Dr.



Registered: 10/03/20
Posts: 278
Loc: Terrapin station, space
Last seen: 3 days, 6 hours
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I am a bit confused about genetic diversity and isolation and gene stability in cultures vs spores.
I have grown some starry night APEs, and their fruits thus far do not have a starry cap. Do I keep looking for a great specimen and then clone it? I started from a culture I bought so I don’t know why it wouldn’t have the desired characteristics.
Second question… SNAPES and other varieties that don’t have heavy sporulation, swabs are a great way to get the spores and share them. But if I am trying to share swabs of SNAPE in the marketplace, should i wait until I get a perfect specimen? I understand how the genetics can be isolated through agar transfers and cloning, but I don’t understand how the diversity gets back in the spores after getting a culture to a monoculture or some selected gene characteristics
-------------------- Noob level III here, thanks to those currently active here and those that went before for all the help!
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chris77
Archaic Revivalist



Registered: 01/17/22
Posts: 1,996
Last seen: 13 hours, 1 minute
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SNAPE works much better as live culture because the stability of the specific genetic trait that brings out the starry caps is unreliable. can work from spores but not necessarily.
what was posted above that is wrong info.
(edit: finally i think it could’ve been a typo)
APE often produces blobs on the first flush (es) because of the overly nutritious mix. later flushes produce more normal looking fruit. high bulk ratios at spawn can help. try 1:4 or higher.
casing can also help, but imo it is most related to overly nutritious mixes
-------------------- the observer is the observed j. krishnamurti
Edited by chris77 (02/21/24 06:11 AM)
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stockw

Registered: 08/31/21
Posts: 533
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Re: Ask Quick Questions, Get Quick Answers [Re: chris77]
#28669181 - 02/21/24 05:41 AM (4 months, 3 days ago) |
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Quote:
chris77 said: SNAPE works much better as live culture because the stability of the specific genetic trait that brings out the starry caps is unreliable. can work from spores but not necessarily.
what was posted above that is wrong info. APE often produces blobs on the first flush (es) because of the overly nutritious mix. later flushes produce more normal looking fruit. high bulk ratios at spawn can help. try 1:4 or higher.
casing can also help, but imo it is most related to overly nutritious mixes
Does this imply you could use half a qt of spawn for a shoebox with 2 qts of coir for better results?
As opposed to 1 qt spawn 4 qts coir which would seem risky if your spawn wasn't 100% perfect but still good enough to expect fruits?
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tree frog
eats bugs



Registered: 09/14/23
Posts: 1,704
Loc: lives in trees
Last seen: 16 hours, 18 minutes
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Re: Ask Quick Questions, Get Quick Answers [Re: stockw] 1
#28669202 - 02/21/24 06:08 AM (4 months, 3 days ago) |
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Those are both 1:4 ratios.
You could split the jar if you wanted. But, if the jar was contaminated you'd end up with two contaminated shoeboxes.
That said, I use 3/4 quart jars often when looking for genetics and basically do what you're asking. Just do it in separate jars.
--------------------

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AndImTheHighOne
Stranger


Registered: 12/06/23
Posts: 64
Last seen: 2 months, 15 days
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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog]
#28669206 - 02/21/24 06:21 AM (4 months, 3 days ago) |
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So, I poured some plates last week that I didn't plan on using for a little while. I hadn't checked for any nasties until today, but clearly I messed up some part of my procedure because this monstrosity was waiting at the very top of my stack in an unwrapped sleeve.

Some very beautiful molds, but mold nonetheless.
My question is how fucked are the rest of my plates if they were chilling below that in the same sleeve?
-------------------- "[Y]ou've already bludgeoned and abused the mycelium so at least do it justice and thoroughly mix it into the substrate " ~ Bigfoot My LAGM 2.024
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chris77
Archaic Revivalist



Registered: 01/17/22
Posts: 1,996
Last seen: 13 hours, 1 minute
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Quote:
AndImTheHighOne said: So, I poured some plates last week that I didn't plan on using for a little while. I hadn't checked for any nasties until today, but clearly I messed up some part of my procedure because this monstrosity was waiting at the very top of my stack in an unwrapped sleeve.

Some very beautiful molds, but mold nonetheless.
My question is how fucked are the rest of my plates if they were chilling below that in the same sleeve?
probably not at all. its impossible to say
-------------------- the observer is the observed j. krishnamurti
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Yahra
Meow


Registered: 03/06/23
Posts: 2,627
Loc: Earth
Last seen: 6 hours, 10 minutes
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Re: Ask Quick Questions, Get Quick Answers [Re: chris77] 1
#28669326 - 02/21/24 08:33 AM (4 months, 3 days ago) |
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The one at 9 o'clock looks like a butthole.
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chris77
Archaic Revivalist



Registered: 01/17/22
Posts: 1,996
Last seen: 13 hours, 1 minute
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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra] 2
#28669333 - 02/21/24 08:35 AM (4 months, 3 days ago) |
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Quote:
Yahra said: The one at 9 o'clock looks like a butthole.

buttmold
-------------------- the observer is the observed j. krishnamurti
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tholos
Stranger



Registered: 03/06/23
Posts: 564
Last seen: 3 days, 2 hours
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Quote:
AndImTheHighOne said: So, I poured some plates last week that I didn't plan on using for a little while. I hadn't checked for any nasties until today, but clearly I messed up some part of my procedure because this monstrosity was waiting at the very top of my stack in an unwrapped sleeve.

Some very beautiful molds, but mold nonetheless.
My question is how fucked are the rest of my plates if they were chilling below that in the same sleeve?
I keep plates in plastic sandwich baggies instead of wrapping them and I’ve seen one plate contaminate and the others sit in the same baggie for weeks and not contaminate. I’d say quarantine that sleeve for an extra time period to ensure that they are clean, but my gut feeling is that you’re probably okay. Depends if the nasties are in your sleeve itself or if that plate was the only thing that got contam in it
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Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 13,178
Loc: Hole
Last seen: 25 minutes, 12 seconds
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Re: Ask Quick Questions, Get Quick Answers [Re: tholos] 1
#28669344 - 02/21/24 08:39 AM (4 months, 3 days ago) |
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They contaminated before or during being wrapped. If the other plates haven’t contaminated, then they’re fine.
-------------------- A Love Letter to New Growers A Guide for New Growers Need Spores? - Sablabs.org Just because your tub contamed, doesn’t mean your attitude has to contam as well.

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