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OfflineTakeatrip
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Registered: 09/10/23
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Techniques for long term storage of mycelium
    #28669015 - 02/21/24 12:11 AM (10 months, 20 days ago)

I made a post the other day about storing mycelium for the long term and I was asking about whether or not you would use wood in your slopes/slants.
A interesting link was given in that you sterilise paper strips, dry them out, place them onto mycelium in a petri dish for around a week or two then dunk them into sterile test tubes with distilled water. Seems simple enough but how the hell do you sterilise thin strips of paper or any paper for that matter without it falling apart and turning into a pile of paper mush?
If anyone has a method for sterilising paper successfully please let me know or if you have experience doing this because I don’t know where to start with getting sterile paper.
Original link is here to the paper strip method ->  https://www.shroomery.org/forums/showflat.php/Number/28161262/page/3

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OfflineWomble
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Registered: 09/12/23
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Re: Techniques for long term storage of mycelium [Re: Takeatrip] * 1
    #28669082 - 02/21/24 02:38 AM (10 months, 20 days ago)

The thread you quoted tells you how to do it.

"First I hydrated paper strips by dunking paper strips in distilled water for a few seconds and sterilizing them for 20 minutes in my trusty presto"

I would personally put the strips in foil and place them in a jar before PC'ing

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OfflineDERRAYLD
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Re: Techniques for long term storage of mycelium [Re: Womble]
    #28669093 - 02/21/24 03:10 AM (10 months, 20 days ago)

The strips stay in the distilled water, you need the distilled water to keep the culture in stasis.

I've got my story back to front, colonize paper and then into sterile water.

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OfflineTakeatrip
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Registered: 09/10/23
Posts: 195
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Re: Techniques for long term storage of mycelium [Re: Womble]
    #28670502 - 02/21/24 09:40 PM (10 months, 20 days ago)

Quote:

Womble said:
The thread you quoted tells you how to do it.

"First I hydrated paper strips by dunking paper strips in distilled water for a few seconds and sterilizing them for 20 minutes in my trusty presto"

I would personally put the strips in foil and place them in a jar before PC'ing



I did read that bit but as you said it just says dunk them in distilled water then into the pressure canner hence why I made this post, it did not have enough detail.
I will attempt what you have suggested to see if it works in a jar in foil that is a good idea, thanks.

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OfflineTakeatrip
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Registered: 09/10/23
Posts: 195
Last seen: 3 months, 14 days
Re: Techniques for long term storage of mycelium [Re: DERRAYLD]
    #28670507 - 02/21/24 09:42 PM (10 months, 20 days ago)

Quote:

DERRAYLD said:
The strips stay in the distilled water, you need the distilled water to keep the culture in stasis.

I've got my story back to front, colonize paper and then into sterile water.



I get what you meant. I will do this once I have my paper strips intact from the above post.

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