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Offline254DEUS254

Registered: 12/09/21
Posts: 75
Loc: Central Texas
Last seen: 22 days, 16 hours
Plate contamination towards the outside
    #28668733 - 02/20/24 07:21 PM (10 months, 21 days ago)

I keep having this issue of contamination showing up on the very outer parts of my plates.. what's going on?! I'm using a vent hood.

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Offlinenormalperson
Stranger
 User Gallery

Registered: 10/31/19
Posts: 785
Last seen: 5 days, 8 hours
Re: Plate contamination towards the outside [Re: 254DEUS254]
    #28668770 - 02/20/24 07:52 PM (10 months, 21 days ago)

Quote:

254DEUS254 said:
I keep having this issue of contamination showing up on the very outer parts of my plates.. what's going on?! I'm using a vent hood.


What do you mean by "vent hood"?

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InvisibleItsLinkNotZelda
Meat Popsicle
Registered: 01/20/24
Posts: 69
Re: Plate contamination towards the outside [Re: normalperson]
    #28668787 - 02/20/24 08:05 PM (10 months, 21 days ago)

Might be where your hand or wrist is hanging over the edge when you are transferring or adding spores/lc.

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Offline254DEUS254

Registered: 12/09/21
Posts: 75
Loc: Central Texas
Last seen: 22 days, 16 hours
Re: Plate contamination towards the outside [Re: normalperson]
    #28668799 - 02/20/24 08:08 PM (10 months, 21 days ago)

Sorry, I get stuck in shitty chef lingo. It's a FloCube laminar flow hood

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Offline254DEUS254

Registered: 12/09/21
Posts: 75
Loc: Central Texas
Last seen: 22 days, 16 hours
Re: Plate contamination towards the outside [Re: ItsLinkNotZelda]
    #28668814 - 02/20/24 08:21 PM (10 months, 21 days ago)

Quote:

ItsLinkNotZelda said:
Might be where your hand or wrist is hanging over the edge when you are transferring or adding spores/lc.




Omg I would never! My hands and wrists are far away from the plate when I'm dropping the LC on the plates :confused: or so I'd like to think... I'm actively trying and concentrating on doing so.

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InvisibleWyoMX
I'm a teapot User Gallery
Registered: 07/06/15
Posts: 2,351
Loc: PNW
Re: Plate contamination towards the outside [Re: 254DEUS254]
    #28668965 - 02/20/24 11:00 PM (10 months, 20 days ago)

Those hoods look pretty legit so unless your filter is ripped or has holes I'd wager its your sterile technique somewhere isn't quite up to par. Is it every single plate? Only ones you inoculated? Only half of them? What are we working with here

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Offline254DEUS254

Registered: 12/09/21
Posts: 75
Loc: Central Texas
Last seen: 22 days, 16 hours
Re: Plate contamination towards the outside [Re: WyoMX]
    #28670258 - 02/21/24 07:19 PM (10 months, 20 days ago)

Quote:

WyoMX said:
Those hoods look pretty legit so unless your filter is ripped or has holes I'd wager its your sterile technique somewhere isn't quite up to par. Is it every single plate? Only ones you inoculated? Only half of them? What are we working with here



Only plates I've inoculated. Plates sat after being poured for about 5 days before that with no signs of contamination.

Since I did pour the plates successfully with no contamination I guess that narrows it down a little. Contaminates falling from my hands or syringes.

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