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Offlinespacetimeham
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Registered: 02/19/24
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Contamination, slime mold in spawn jars, help please
    #28667395 - 02/19/24 08:38 PM (4 months, 4 days ago)

Hi. So I've been cultivating for a few years now with varying degrees of success. I've had great results, mediocre results, and many failures to launch. My current issue is with spawn jars becoming contaminated with slime mold. Or something. I don't know the exact contaminant, but it's been consistent, as in the same symptoms appearing over the last few attempts. I am frustrated.

I begin with a single quart mason jar with prepped/sterilized oats which are inoculated with a spore syringe. I never have any issues here. Then I break up that colonized grain and transfer it into six other quart mason jars of the same sterilized oats. This seems to be where the problems arise. The same problem. The grain gets slimy and the mycelium fails to grow and colonize the jar.

The oats I use are hydrated by initially boiling them for 10ish minutes and are then left to soak in water for 24 hours, and then sterilized in a pressure cooker for about 2 hours. Then the jars are sealed until it's time for the transfer from that initial clean and colonized master jar.

Admittedly I've become complacent in my past few attempts. I.E. making the transfer wearing clothes that haven't been washed in a week or more and not wearing a mask. But this time... I tried to use extra precautions. I transferred the master to the six other jars right after I showered. I was naked and wearing an N95 mask and latex gloves cleaned with rubbing alcohol. On a kitchen countertop that I had wiped down with disinfectant, not to mention wiping down the jars themselves with rubbing alcohol. The air was still.

Yet, half of the six jars I transferred healthy spawn into are showing signs of this same slime mold (I'm not sure if that's the proper name or not, but that's how it's appearing). All the jars started off colonizing well. Then I gave them a shake to speed up colonization. And now half the jars are showing no more signs of growth because they're apparently being suffocated/stifled by something slimy. It's not discolored at all. The grain just appears oversaturated (despite the grain not being too wet, or so I thought?) and it's getting slimy and starting to form bubbles in the most obvious areas of contamination.

So... my question is, what is the best way to avoid this from happening again? I've had numerous successful grows in the past using this same process, but my last few attempts have all been foiled by the problem I just described above. And it is f'ing frustrating.

Does anyone have any thoughts or recommendations on how to remedy this problem? Or are there any existing threads one could point me to?

Thanks for reading.

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OfflineCocaineBuffet
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Re: Contamination, slime mold in spawn jars, help please [Re: spacetimeham] * 1
    #28667447 - 02/19/24 09:45 PM (4 months, 4 days ago)

Quote:

spacetimeham said:
Hi. So I've been cultivating for a few years now with varying degrees of success. I've had great results, mediocre results, and many failures to launch. My current issue is with spawn jars becoming contaminated with slime mold. Or something. I don't know the exact contaminant, but it's been consistent, as in the same symptoms appearing over the last few attempts. I am frustrated.

I begin with a single quart mason jar with prepped/sterilized oats which are inoculated with a spore syringe. I never have any issues here. Then I break up that colonized grain and transfer it into six other quart mason jars of the same sterilized oats. This seems to be where the problems arise. The same problem. The grain gets slimy and the mycelium fails to grow and colonize the jar.

The oats I use are hydrated by initially boiling them for 10ish minutes and are then left to soak in water for 24 hours, and then sterilized in a pressure cooker for about 2 hours. Then the jars are sealed until it's time for the transfer from that initial clean and colonized master jar.

Admittedly I've become complacent in my past few attempts. I.E. making the transfer wearing clothes that haven't been washed in a week or more and not wearing a mask. But this time... I tried to use extra precautions. I transferred the master to the six other jars right after I showered. I was naked and wearing an N95 mask and latex gloves cleaned with rubbing alcohol. On a kitchen countertop that I had wiped down with disinfectant, not to mention wiping down the jars themselves with rubbing alcohol. The air was still.

Yet, half of the six jars I transferred healthy spawn into are showing signs of this same slime mold (I'm not sure if that's the proper name or not, but that's how it's appearing). All the jars started off colonizing well. Then I gave them a shake to speed up colonization. And now half the jars are showing no more signs of growth because they're apparently being suffocated/stifled by something slimy. It's not discolored at all. The grain just appears oversaturated (despite the grain not being too wet, or so I thought?) and it's getting slimy and starting to form bubbles in the most obvious areas of contamination.

So... my question is, what is the best way to avoid this from happening again? I've had numerous successful grows in the past using this same process, but my last few attempts have all been foiled by the problem I just described above. And it is f'ing frustrating.

Does anyone have any thoughts or recommendations on how to remedy this problem? Or are there any existing threads one could point me to?

Thanks for reading.




Here is your first issue.

I didn't read the rest of the OP after that.

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Offlinespacetimeham
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Registered: 02/19/24
Posts: 9
Last seen: 3 months, 22 days
Re: Contamination, slime mold in spawn jars, help please [Re: CocaineBuffet]
    #28667491 - 02/19/24 10:41 PM (4 months, 4 days ago)

Well, aren't you friendly... Mister I have 3,501 posts. Please forgive me for creating a new account tonight to ask some questions. If you quit reading after that then why did you even bother to comment? You don't know my process or why I try to keep the materials I use to a minimum. And since you didn't bother to read beyond that, what do you know? More than I do I'm sure, hence the initial question. Why not offer some advice since you're supposedly a 'trusted cultivator'? Thanks for nothing. Offer advice or just don't say anything at all. NEXT

Edited by spacetimeham (02/20/24 03:55 PM)

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Invisiblemeta_mmxxii
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Re: Contamination, slime mold in spawn jars, help please [Re: spacetimeham] * 2
    #28667498 - 02/19/24 10:50 PM (4 months, 4 days ago)

Quote:

spacetimeham said:
Well, aren't you friendly... Mister I have 3,501 posts. Please forgive me for creating a new account tonight to ask some questions. With all due respect, fuck you. If you quit reading after that then why did you even bother to comment? You don't know my process or why I try to keep the materials I use to a minimum. And since you didn't bother to read beyond that, what do you know? More than I do I'm sure, hence the initial question. Why not offer some advice since you're supposedly a 'trusted cultivator'? Fuck your pretentiousness. Thanks for nothing. Offer advice or just don't say anything at all. NEXT



If you have been culting for 2 years, then you should know by now that shooting spore syringe into anything is asking for trouble, someone points this out to you and you get upset?
Did you not learn anything in 2 years?
I am pretty sure he stopped reading at where he highlighted because there was no point in reading what you said after wards.
I too stopped reading at that point. You're issue is you injected dirty spore syringe into sterilized grain.
I only have 690 posts and I know better than to shoot mss into grain.
You may want to rethink your workflow. :shrug:


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Offlinespacetimeham
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Registered: 02/19/24
Posts: 9
Last seen: 3 months, 22 days
Re: Contamination, slime mold in spawn jars, help please [Re: meta_mmxxii]
    #28667506 - 02/19/24 11:03 PM (4 months, 4 days ago)

To be fair, the point was not clear. Tell me more... dirty spore syringes? No, I did not know that and the last reply did not even mention dirty spores. HENCE THE ASK FOR HELP. That's why I got upset. No answers to my question. I've learned lots in the past 2 years, thank you very much. But now I'm stuck and frustrated and, against my nature, I'm asking for help. And so... how should I adjust my workflow?

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OfflineSkropi
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Registered: 11/01/23
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Re: Contamination, slime mold in spawn jars, help please [Re: spacetimeham]
    #28667508 - 02/19/24 11:05 PM (4 months, 4 days ago)

I am sorry for being harsh, but I also stopped reading at the point everyone else did, and I am the least experienced person here. Cocainebuffet just pointed out your issue, nothing more, nothing less. Shooting spores to grains is risky. If you like shooting spores, I do personally, just go with the PF Tek mate, you will have decent results there.


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Offlinespacetimeham
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Registered: 02/19/24
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Re: Contamination, slime mold in spawn jars, help please [Re: Skropi]
    #28667520 - 02/19/24 11:18 PM (4 months, 4 days ago)

Ugh. Whatever. y'all have short attention spans apparently. So do I. I gave details. But you couldn't read them. Apparently. I was asking for help and all I got was a bunch of assholes that couldn't read an entire post. And so... at this point, I have multiple people telling me spore syringes are risky... would anyone mind pointing me to a better alternative? Honest request here

Edited by spacetimeham (02/19/24 11:26 PM)

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Offlinespacetimeham
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Registered: 02/19/24
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Re: Contamination, slime mold in spawn jars, help please [Re: Skropi]
    #28667525 - 02/19/24 11:25 PM (4 months, 4 days ago)

I've moved past the pf tek a long time ago, mate. Thanks tho. What else you got?

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OfflineDERRAYLD
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Re: Contamination, slime mold in spawn jars, help please [Re: spacetimeham] * 1
    #28667530 - 02/19/24 11:29 PM (4 months, 4 days ago)

So you moved past pf tek and still use spore syringes?
Have you moved to agar and focused on axenic spawn for bulk?

You're super confrontational, take a breath and use the input you received here rather than immediately going on the offensive

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Offlinespacetimeham
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Registered: 02/19/24
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Re: Contamination, slime mold in spawn jars, help please [Re: DERRAYLD]
    #28667534 - 02/19/24 11:39 PM (4 months, 4 days ago)

I'm just frustrated. With my spore syringes... why are they so dirty? And what is the alternative? I'm frustrated because people aren't even considering my entire post, but still telling me, you're doing this wrong. Syringes are dirty? Tell me why! Please.

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OfflineDERRAYLD
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Re: Contamination, slime mold in spawn jars, help please [Re: spacetimeham]
    #28667536 - 02/19/24 11:42 PM (4 months, 4 days ago)

Spores are inherently dirty by their very nature.
Anything exposed to the air will be contaminated to some extent because we're surrounded by mold spores.

The standard approach is to germinate spores on agar and then to transfer sectors to new plates to clean up the mycelium so that you get a uniform contamination free plate.

Once you have a clean plate you cut wedges and transfer them to grain jars.

This approach will get you clean grain jars that will be easy to bulk in a monotub with coir.

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Offlinespacetimeham
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Re: Contamination, slime mold in spawn jars, help please [Re: DERRAYLD]
    #28667553 - 02/20/24 12:02 AM (4 months, 4 days ago)

Hmm... thanks homies. I appreciate all your time. Sorry I had to get confrontational to get it! Agar is the next step I spose... have read about it but have not implemented it yet. Hence the original question. Thank you. I guess I didn't realize spores were so inherently dirty. So in a round about way... thanks y'all for answering my question (kinda). Hence the original question! Glad I got something out of it. But really, y'all are lazy, can't even read an entire post can you? :smile:

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Offlinespacetimeham
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Re: Contamination, slime mold in spawn jars, help please [Re: spacetimeham] * 1
    #28667556 - 02/20/24 12:05 AM (4 months, 4 days ago)

Thanks for the responses everybody and sorry for being a, I dunno, curmudgeon. Have a good night!

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OfflineBajazly
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Re: Contamination, slime mold in spawn jars, help please [Re: spacetimeham] * 4
    #28667998 - 02/20/24 10:44 AM (4 months, 3 days ago)

I got to the very same point CB got to and thought, reading the rest of this is a huge waste of time, but I did and then there was this, "I transferred the master to the six other jars... On a kitchen countertop that I had wiped down with disinfectant, not to mention wiping down the jars themselves with rubbing alcohol. The air was still."

You did this open air??

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InvisibleLadysKnight
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Re: Contamination, slime mold in spawn jars, help please [Re: spacetimeham] * 3
    #28668110 - 02/20/24 12:22 PM (4 months, 3 days ago)

Quote:

spacetimeham said:
Fuck your pretentiousness. Thanks for nothing. Offer advice or just don't say anything at all.




My advice is to do your own reading since you don't know how to talk to people

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OfflineSonomaFungi_707
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Re: Contamination, slime mold in spawn jars, help please [Re: spacetimeham] * 3
    #28668128 - 02/20/24 12:44 PM (4 months, 3 days ago)

Re: “dirty” syringes…. I just happened to shoot some B+ spore syringe juice onto agar the other day. This syringe definitely has cubensis spores in it (based on other projects underway from the same source), but as you can see in this plate it also has bacteria. Within the next few days or a week, I expect to see some mycelium starting to grow outward from this bacterial splotch (where the drop of spore solution landed on the plate). From there, I will transfer some clean mycelium to a new plate (in a Still Air Box!), and voila - no more bacteria in my culture.

It’s not so much that you’ve been doing anything wrong for the last couple years. It’s just that you’ve been lucky. Now the odds are catching up with you and it’s time to revisit your methods.


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InvisibleOctopusDisco
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Re: Contamination, slime mold in spawn jars, help please [Re: SonomaFungi_707]
    #28668160 - 02/20/24 01:06 PM (4 months, 3 days ago)

Quote:

SonomaFungi_707 said:
It’s not so much that you’ve been doing anything wrong for the last couple years. It’s just that you’ve been lucky. Now the odds are catching up with you and it’s time to revisit your methods.





:whathesaid:

From a stickied thread:

Quote:

Stipe-n Cap said:
...

Can one produce clean cultures while using any of the contraptions covered by this post? absolutely!

Cultivators with little to no experience can produce clean cultures in the open air without a box-fan flowhood, positive pressure box, shmuvbox, clean bench, SAB, or any other known cultivation tool. Humans have been growing mushrooms for thousands of years.

One can produce clean germination plates by shooting, or streaking multispore solution onto agar; clean spawn can be produced by spraying MS-solution liberally over cereal grains. It's incredibly easy to produce cultures or spawn, and by extension to grow mushrooms; anything can be achieved with a little luck.

We're not operating in a CDC clean room environment with virulent/pathogenic viruses or bacteria, cultivators can get away with an awful lot of shenanigans on a small enough scale.

Can you produce mushrooms while using a: shmuvbox, positive pressure box, boxfan flowhood, glovebox, etc? Yes!

Everything "works" so long as you possess the appropriate genetic starting materials like spores, or live mycelium, and a substrate upon which they will grow.

Cubensis will grow on manure, cigarette butts, bibles, fancy underwear, rags, etc. Just because you can, doesn't mean you should.

...

Use the right tools for the right job, employ the proper procedures and techniques.





When people stopped reading your post at the mention of MSS to grain, it wasn't because of laziness or rudeness; it was because they've been around for long enough to know that until  that is addressed, there isn't an alternative explanation that is worth investigating.

Yes, shooting MSS to grain CAN work--as evidenced by your successful grows--but The Shroomery Way says that you should never do this.

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InvisibleMycolorado
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Re: Contamination, slime mold in spawn jars, help please [Re: OctopusDisco] * 1
    #28668185 - 02/20/24 01:19 PM (4 months, 3 days ago)

I read the whole thing but figured I’d let others point out the syringe issue.  That said, it doesn’t sound like op is using a SAB and working on the counter.  If so, would be another potential vector.

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Offlinedubbtronix12345
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Re: Contamination, slime mold in spawn jars, help please [Re: Mycolorado]
    #28669672 - 02/21/24 12:23 PM (4 months, 2 days ago)

I used to do ms to grain and I lost two tubs and one grain bag before switching to agar. The last grow I did was agar to grain and it worked very well

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Invisiblemeta_mmxxii
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Re: Contamination, slime mold in spawn jars, help please [Re: dubbtronix12345] * 1
    #28669701 - 02/21/24 12:45 PM (4 months, 2 days ago)

And just a little more advice to you OP, calling people assholes and being abrasive will not help your cause whatsoever. :shrug:


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Trusted Cultivators Teks

Ultimate Tek Compendium

The Hitchhikers Guide

         

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

        FRENCHPRESS GANG

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