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Offlinemore4u2c
Mad Hatter

Registered: 07/08/14
Posts: 526
Last seen: 4 months, 5 days
first time doing LC do i have contams or just need to stir more often?
    #28666975 - 02/19/24 02:08 PM (10 months, 22 days ago)

so far all my LC has been doing really good maybe i have beginners luck? but suddenly today i have 2 jars where the myc is no longer sinking to the bottom of the jar and is now floating on the top. is this a sign of contam or is it some how growing so big its becoming buoyant? lol let me know what yall think. the B+ is just floating up and the JMF is floating up and has some white myc poking out ontop of the water. i use a flow hood and i use a new sterile syringe and needle on every jar so there is no cross contamination so its kinda weird how 2 different jars are suddenly doing the same floating thing. (pics have B+ lable shown one with floating myc and the other that doesnt have floating myc and then 2 other jars with no lable shown is the JMF thats floating with white myc popping above the liquid surface)  Also its been around 24 to 30 hours since their last stir plate stirring.





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Offlinemore4u2c
Mad Hatter

Registered: 07/08/14
Posts: 526
Last seen: 4 months, 5 days
Re: first time doing LC do i have contams or just need to stir more often? [Re: more4u2c]
    #28668210 - 02/20/24 01:41 PM (10 months, 21 days ago)

just spun up the one that has the floating white island of mass on top of the liquid and it broke up into chunks like it looks like little chunks of white cheese spinning around in the LC is this just really thick myc or some contamination im leaning its some sort of contamination?

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OfflineSmellit
Stranger
Registered: 01/29/24
Posts: 66
Last seen: 10 days, 10 hours
Re: first time doing LC do i have contams or just need to stir more often? [Re: more4u2c]
    #28668297 - 02/20/24 02:41 PM (10 months, 21 days ago)

Test it on agar to find out. This needs to be done to confirm if the culture is contamination free.

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