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OfflineRukusS
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New to Agar, lions mane fail?
    #28664970 - 02/18/24 06:39 AM (4 months, 5 days ago)



My first time using agar. Used a still air box, sterilized everything with alcohol, used a sterile scalpal to cut a small chunk of mycelium from a lions mane fruiting block. 2 days later agar plates looks like this, I have heard lions mane grows out weird on agar but I'm assuming this is mold in my case correct?

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Offlinetaku
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Re: New to Agar, lions mane fail? [Re: Rukus]
    #28665330 - 02/18/24 10:42 AM (4 months, 5 days ago)

i would assume that's mold yes.  success would look like myc forming from your sample in the center.  you may get some contam on the edges, but you would slice out a healthy portion and grow it out on new agar.

try again. i haven't personally heard of agar work being done in a sab, but that doesn't mean it doesn't work or can't be successful.  you may just end up with more contaminated dishes per successful dish.


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OfflineRukusS
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Re: New to Agar, lions mane fail? [Re: taku]
    #28665337 - 02/18/24 10:47 AM (4 months, 5 days ago)

Ok, agar work generally needs a flow hood then?

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OfflineChtouxhu
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Re: New to Agar, lions mane fail? [Re: Rukus]
    #28665384 - 02/18/24 11:42 AM (4 months, 5 days ago)

Quote:

Rukus said:
Ok, agar work generally needs a flow hood then?




Not at all; I personally do all my agar work in a small SAB, I only had like two contaminations in the last three or four months. Keep in mind I work with borosilicate glass petri though, I don't pour sterilized agar on sterile plates. I just put the whole unsterilized plates inside the pressure cooker with agar already poured.

A SAB also allows you to transfer healthy mycelium from a mold-infected plate. A flow hood would blow mold spores everywhere.

Here's a picture of a pioppino plate in my messy SAB:



All of these plates were done in the same SAB:

Shaggy mane


Button mushroom:



King oyster pinning in-vitro:


Various plates and tubes in the SAB:


Edited by Chtouxhu (02/18/24 11:49 AM)

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InvisibleTPython
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Re: New to Agar, lions mane fail? [Re: Chtouxhu] * 1
    #28665535 - 02/18/24 01:21 PM (4 months, 5 days ago)

I also do all my agar work in a SAB with high success rate when using proper sterile procedure.

But I would also opt for taking a tissue sample from the inside of the fruiting body rather than a piece of the block. Rip the fruit in half and then cut out a small chunk from the middle most section. Open your plate for just enough time to get your sample in there while using slow and deliberate movements.

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OfflineRukusS
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Re: New to Agar, lions mane fail? [Re: TPython]
    #28665550 - 02/18/24 01:34 PM (4 months, 5 days ago)

Thank you for the advice everyone !

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Offlinetaku
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Re: New to Agar, lions mane fail? [Re: Rukus]
    #28666405 - 02/19/24 07:16 AM (4 months, 4 days ago)

listen to these people, i don't do much sab stuff. :smile:


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InvisibleTPython
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Re: New to Agar, lions mane fail? [Re: taku] * 1
    #28666772 - 02/19/24 11:49 AM (4 months, 4 days ago)

Stop rubbing it in to us SAB plebeians Taku..
:trustme:

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Offlinetaku
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Re: New to Agar, lions mane fail? [Re: TPython]
    #28667452 - 02/19/24 09:48 PM (4 months, 4 days ago)

:eyesonyou:


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