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SWBZA


Registered: 05/09/23
Posts: 304
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Agar plate read request: water agar education 1
#28661522 - 02/16/24 01:30 AM (10 months, 25 days ago) |
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Hi Shroomery.
I please need some education. This is a water agar plate with a wedge from a normal LME clone plate. You can see the original clone material in the transfer cut - I transferred a little baby. You can see where the transfer was placed originally: look at the growth on the plate (left of the photo, in the middle), and you can see the wedge slipped across the plate and came to rest at the bottom edge of this petri dish. You only see part of the dish as I had to play with edge lighting to get an at least somewhat clear photo. That black sector bottom left is the screen of the desk lamp - ignore.

On this second photo I went as close-up as I could on the top-right part of the plate - just below what might be a contam island. That island is not relevant here, I refer to it just to orient us and to link the two photos. What I tried to capture here in this second photo is a "lightning strike" of something growing - starting bottom-right of the photo, growing to the top left, in the direction of the island.

My question in this thread: is there anything other than the myc we love, that grows like this on a plate? The "lightning" looks to me like thin "branches" of something reaching out. Is there some form of mould that does this? I assume bacteria don't make wispy lighting like this, right? The bacteria on plates I've seen grow more like "wet arms" reaching out, not with thin things in the middle of the "arms". Ultimately I want to know whether this is clean growth (excepting that island, of course, I think it is dodge), but I'm more interested in learning how to read it myself than getting a clean / not clean here. This is also a water agar plate - not something I'm familiar with.
Hoping for some detailed learning, hoping it helps others, and thanks in advance. All the best with your individual grows.
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fiddle_head
fettucine embargo



Registered: 08/05/08
Posts: 3,694
Loc: moon
Last seen: 17 hours, 17 minutes
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Re: Agar plate read request: water agar education [Re: SWBZA] 1
#28664744 - 02/17/24 10:37 PM (10 months, 23 days ago) |
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I’m not entirely sure about water agar but it looks sus. I wish I could give you some more detailed learning but what I will say is this: those white flecks are enough to scare me away from it. IMO this plate is too sus to be viable for anything other than donor via an aerial transfer or clean biospy.
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SWBZA


Registered: 05/09/23
Posts: 304
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Re: Agar plate read request: water agar education [Re: fiddle_head]
#28664770 - 02/17/24 11:17 PM (10 months, 23 days ago) |
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Thanks, bud, guess that's why I asked. I'm getting some idea of what things look like on normal agar, so either this a new learning for me of what "normal" looks like on water agar, or it's doge. I'm going with dodge. Have a great day!
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3.A.M
Boop!



Registered: 10/17/22
Posts: 2,090
Loc: Oz
Last seen: 8 hours, 17 minutes
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Re: Agar plate read request: water agar education [Re: SWBZA]
#28664850 - 02/18/24 02:18 AM (10 months, 23 days ago) |
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Those outer edges, towards the middle of the first pic look like classic myc on water agar to me I’d at least transfer from it to nutrient agar and see.
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SWBZA


Registered: 05/09/23
Posts: 304
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Re: Agar plate read request: water agar education [Re: 3.A.M]
#28664870 - 02/18/24 03:56 AM (10 months, 23 days ago) |
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Quote:
3.A.M said: Those outer edges, towards the middle of the first pic look like classic myc on water agar to me I’d at least transfer from it to nutrient agar and see.
Solid plan, thanks. Easy enough to do.
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pacmanbreed


Registered: 10/12/16
Posts: 3,829
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Re: Agar plate read request: water agar education [Re: SWBZA]
#28664914 - 02/18/24 05:30 AM (10 months, 23 days ago) |
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Just to add from my limited exp trying nuteless agar.
Water agar having less to no simple sugar is mostly good against bacteria, won't prevent molds - slow it abit I believe, the same goes with mycelium. I let em grow, then effortly try my best to get an ideal wedge that is translucent in growth in the most outer edge, this tend to be vigorous(expanding) - finding nutes to munch.
Interms piggy backing bacteria, I had Much success With katbusa and Ferather tea agar (antibiotic alternative) along with Josex pucks.
hot pour agar is another alternative, but haven't tried it yet.
Edited by pacmanbreed (02/18/24 05:54 AM)
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SWBZA


Registered: 05/09/23
Posts: 304
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Re: Agar plate read request: water agar education [Re: pacmanbreed] 1
#28664951 - 02/18/24 06:24 AM (10 months, 23 days ago) |
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Quote:
pacmanbreed said: Just to add from my limited exp trying nuteless agar.
Water agar having less to no simple sugar is mostly good against bacteria, won't prevent molds but slow down its growth I believe, the same goes with mycelium. I let em grow, then effortly try my best to get an ideal wedge that is translucent in growth in the most outer edge, this tend to be vigorous(expanding) - finding nutes to munch.
Interms piggy backing bacteria, I had Much success With katbusa and Ferather tea agar (antibiotic alternative), hot pour agar is another, but haven't tried it yet.
Nice one, thanks, and thanks for the links. I'll do some reading. Cheers!
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