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OfflineFunfarm
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Over ripe tissue * 1
    #28660737 - 02/15/24 12:21 PM (10 months, 26 days ago)

Got a fruit that I'm wanting to tissue sample both to liquid culture and a handful of plates. They will be on the cake just a couple days longer than I would like due to my poor timing. The veil broke yesterday and I'll be taking samples tomorrow, while the type is PE. I don't see any spore droppings as I'm not to concerned with that anyways, just trying to get more procedural practice in. Im more concerned with tissue that's overripe to culture.  As dumb as that sounds I think I answered my own query. I'm thinking if it's still attached and receiving moisture the tissue for attempted cloning will be of no fact in terms of ripeness, a couple days won't matter?

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InvisibleWyoMX
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Re: Over ripe tissue [Re: Funfarm] * 2
    #28660742 - 02/15/24 12:24 PM (10 months, 26 days ago)

Yep you'll be fine. Also just to add that in theory it sounds good to take a sample directly to LC but alot of times you end up with a little bacteria or ride along. So feel free to experiment as lc is pretty cheap to make but make sure you keep an eye on it.

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OfflineFunfarm
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Re: Over ripe tissue [Re: WyoMX]
    #28660762 - 02/15/24 12:46 PM (10 months, 26 days ago)

Yes, yes, excellent, 👌 thank you.
Now that I have a SAB and dish plate wrap technique both adapted for my physical limitations. I find myself more motivated to work with plates, where before I was blowing it off. The culture jar is just to see if I can do it, in case I find myself in a pinch (no plates on hand, etc.) for tissue sampling. I watched a young lady on the utuber channel take fruit samples by needle inject to broth suspension and then later make small drops of grown out myc on Agar. I realize it would be easier to go directly to plates with tissue and transfer from there as needed. I really just want to be able to see that I can accomplish a couple different methods in my hobby level of mycology. I'm kind of considering it learning modules in a combined undergrad level biology/chemistry course. I like to know I can successfully accomplish a course module 🤠. Anyways thank you again for the anxiety soothing response, I do trust you to a most great day.

Final note:The next modules consist of correct pressure cooking procedures with making broth and Agar plate manufacturing.

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Invisiblejoze
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Re: Over ripe tissue [Re: WyoMX] * 2
    #28660768 - 02/15/24 12:51 PM (10 months, 26 days ago)

Quote:

WyoMX said:
Yep you'll be fine. Also just to add that in theory it sounds good to take a sample directly to LC but alot of times you end up with a little bacteria or ride along. So feel free to experiment as lc is pretty cheap to make but make sure you keep an eye on it.



:whathesaid:


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OfflineFunfarm
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Re: Over ripe tissue [Re: joze]
    #28661053 - 02/15/24 04:47 PM (10 months, 26 days ago)

Yes, excellent thank you

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OfflineFunfarm
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Re: Over ripe tissue [Re: Funfarm] * 2
    #28663653 - 02/17/24 09:53 AM (10 months, 24 days ago)

I don't want to over post the board in multiple spots, as this is the last comment concerning tissue biopsy by needle to jar broth suspension... yeah, not going to pursue it. Quoting another member "Agar is your friend". I have been putting off sab and plate work for over a year. I suck at it, but getting better. I learn a mistake everytime,🔥 and I think by process of error elimination I will get something viable eventually.

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Offlinefractadactyl
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Re: Over ripe tissue [Re: Funfarm] * 1
    #28663672 - 02/17/24 10:12 AM (10 months, 24 days ago)

You can even clone dry tissue, i actually just brought an old clone back from the dead last month.
I sent a plate of that clone to a friend and had forgotten it was my last one.
It's back in the library and thriving now.


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OfflineFunfarm
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Re: Over ripe tissue [Re: fractadactyl] * 1
    #28663740 - 02/17/24 10:55 AM (10 months, 24 days ago)

Nice! Either dry or fresh, I would like to see a viable plate in the future. Good information to know concerning replication of old tissue.

To bad we can't do that with other biological cellular make ups. Like as if there were super sensitive secret labs underground. Time to get out the aluminum foil hat.

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Offlinefractadactyl
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Re: Over ripe tissue [Re: Funfarm] * 1
    #28663753 - 02/17/24 11:00 AM (10 months, 24 days ago)

I should mention that it only works with dried fruit if you didn't use heat in the drying process, for your reference.
I'm sure you'll have a viable plate.
Might take a couple transfers, sometimes clones have bacteria but it's not too hard to get away from.


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OfflineFunfarm
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Re: Over ripe tissue [Re: fractadactyl]
    #28663806 - 02/17/24 11:31 AM (10 months, 24 days ago)

No we air dry here with paper plates and napkins in a humid basement, would of been perfect for cannabis drying (the slow wick) no chlorophyll in the buds, just delicious yummy. It's been taking so long that I have to flip the fruits about once every 3 days and put down fresh napkins. Believe me I don't like finger fumbling the fruit so much but grandma doesn't want the plates upstairs around the pets and visiting guests where it's dryer and warmer. Im not trying to start a pissing war with other members but I prefer the air dry vs. manual dehydration. I feel the chemical process of water leaving the body in a slower manner, leaves more active ingredients. In all fairness I make this statement with zero dehydrator experience and with my experience with marijuana. If I can add, we cultivated cannabis in a very arid dry climate, the desert of the Southwest. The humidity is so low that when you would hang a top shelf harvested bud it would be cracker dry in a couple days, taste and smoke like shit. I found that bringing the drying room up to 60% relative humidity with a digital humidifier given the actual rh is 30% at best. This allowed the moisture to wick out slowly as chlorophyll attaches to water molecules and the slower they dry the sweeter the product. With shrooms I'm taking the same mindset, although a different genius species and I maybe wrong I just can't see baking fruits. I realize culinary shrooms are used differently but the local gourmet shroom store they air dry too, different process involving food grade plastic grates and a fan. Didn't intend to blow the ego horn of experience just sharing and opening up dialogue concerning the topic of drying.

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Invisibleelasticaltiger
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Re: Over ripe tissue [Re: Funfarm] * 1
    #28663819 - 02/17/24 11:39 AM (10 months, 24 days ago)

Quote:

Funfarm said:
No we air dry here with paper plates and napkins in a humid basement, would of been perfect for cannabis drying (the slow wick) no chlorophyll in the buds, just delicious yummy. It's been taking so long that I have to flip the fruits about once every 3 days and put down fresh napkins. Believe me I don't like finger fumbling the fruit so much but grandma doesn't want the plates upstairs around the pets and visiting guests where it's dryer and warmer. Im not trying to start a pissing war with other members but I prefer the air dry vs. manual dehydration. I feel the chemical process of water leaving the body in a slower manner, leaves more active ingredients. In all fairness I make this statement with zero dehydrator experience and with my experience with marijuana. If I can add, we cultivated cannabis in a very arid dry climate, the desert of the Southwest. The humidity is so low that when you would hang a top shelf harvested bud it would be cracker dry in a couple days, taste and smoke like shit. I found that bringing the drying room up to 60% relative humidity with a digital humidifier given the actual rh is 30% at best. This allowed the moisture to wick out slowly as chlorophyll attaches to water molecules and the slower they dry the sweeter the product. With shrooms I'm taking the same mindset, although a different genius species and I maybe wrong I just can't see baking fruits. I realize culinary shrooms are used differently but the local gourmet shroom store they air dry too, different process involving food grade plastic grates and a fan. Didn't intend to blow the ego horn of experience just sharing and opening up dialogue concerning the topic of drying.




Your idea about a slower drying process being better hasn't been demonstrated here and air, light and moisture are going to be the number one factors in degrading potency.

This is why we dehydrate "manually" so we can move the fruits from exposure to a sealed container as quickly as possible to remove as many of those factors as we can.

That being said, you can speed up your process by just putting a fan to blow air over them next to the fruits without any added heat. I used to do a box fan with a big sheet pan and paper towels and that would do the job in about 2 days.


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InvisibleWyoMX
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Re: Over ripe tissue [Re: Funfarm] * 1
    #28663825 - 02/17/24 11:42 AM (10 months, 24 days ago)

Quote:

Funfarm said:
No we air dry here with paper plates and napkins in a humid basement, would of been perfect for cannabis drying (the slow wick) no chlorophyll in the buds, just delicious yummy. It's been taking so long that I have to flip the fruits about once every 3 days and put down fresh napkins. Believe me I don't like finger fumbling the fruit so much but grandma doesn't want the plates upstairs around the pets and visiting guests where it's dryer and warmer. Im not trying to start a pissing war with other members but I prefer the air dry vs. manual dehydration. I feel the chemical process of water leaving the body in a slower manner, leaves more active ingredients. In all fairness I make this statement with zero dehydrator experience and with my experience with marijuana. If I can add, we cultivated cannabis in a very arid dry climate, the desert of the Southwest. The humidity is so low that when you would hang a top shelf harvested bud it would be cracker dry in a couple days, taste and smoke like shit. I found that bringing the drying room up to 60% relative humidity with a digital humidifier given the actual rh is 30% at best. This allowed the moisture to wick out slowly as chlorophyll attaches to water molecules and the slower they dry the sweeter the product. With shrooms I'm taking the same mindset, although a different genius species and I maybe wrong I just can't see baking fruits. I realize culinary shrooms are used differently but the local gourmet shroom store they air dry too, different process involving food grade plastic grates and a fan. Didn't intend to blow the ego horn of experience just sharing and opening up dialogue concerning the topic of drying.




The thing is that weed and shrooms are completely different. The biggest thing that actively kills potency on your mushrooms is moisture. There's been actual studies done on this at actual labs and I've seen at least 2 of them. They 100% retain more actives when being dried faster. Your right with weed but that's to preserve terpenes and other cannaboniods. With mushrooms you wanna dry as fast as possible because moisture absolutely degrades the actives faster. Up to 200°F is where you see degradation from heat and even then it's negligible.

Your free to do things your way but it goes against every study I've seen on the topic.

One thing I really recommend is to realize mushrooms ≠ weed. Like at all. We're talking fungi vs plants. Just completely different.

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OfflineFunfarm
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Re: Over ripe tissue [Re: WyoMX]
    #28663903 - 02/17/24 12:38 PM (10 months, 24 days ago)

Quote:

WyoMX said:

One thing I really recommend is to realize mushrooms ≠ weed. Like at all. We're talking fungi vs plants. Just completely different.




Please see where I mentioned it's a different genius species, but I will take into account what you replied with the unequal sign. I have seen cultivators on this very site going back to year 2000 who are not advocates of manual dehydration. I'm not arguing it because I've never done it. I'm just supporting my personal logic. If the active compounds stay intact while drying either slow or fast dry it doesn't really matter. Here's my further position I don't have access nor care to learn spectrophotometric methods. To analyze phenolic compounds, carbohydrates, and sterols, and high-performance liquid chromatography (HPLC) used to identify individual compounds in a slow dry vs. fast dry gram 2 gram. I can say that no one, not one receipeant of free magic from our tubs have complained about taste or effect from plate drys. I don't want to be a asshole as I appreciate your present and past input and hope they don't stop but can you provide a link for the studies you referred to as I would entertain a dehydrator. Thank you both as I do respect the trusted cultivators badge as I realize they aren't just handed out, yet I remain skeptical please do not take as dismissal as I'm open to the purchase of a dehydrator.

Edited by Funfarm (02/17/24 12:55 PM)

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OfflineFunfarm
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Re: Over ripe tissue [Re: Funfarm]
    #28663953 - 02/17/24 01:21 PM (10 months, 24 days ago)

Not to be a complete asshole but I'm just sharing literature study as you but forth, this one from study in Prague, Czech Republic. Study revised in 2020, by the Forensic Laboratory of Biologically Active Substances, Dept. of Chemistry of natural substances along with contributions of other entities to include Neurobiology.  Please find on the second screen shot, the second highlighted paragraph, and the first sentence with in. While I would be very interested in looking at another study and appreciate the experience of others. Please don't think I'm trying to piss farther than you, for the record I am torn between the slow dry and manual dehydration methods given my lack of education and experience.


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InvisibleWyoMX
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Re: Over ripe tissue [Re: Funfarm] * 1
    #28663967 - 02/17/24 01:30 PM (10 months, 24 days ago)

I've seen that study as well. It shows heat doesn't do much when being stored but doesn't show that room drying at room temperature is the best that's just how they did it. If I come across the other studies I've seen ill post them but I'll be honest I'm not gonna go outta my way to find them. I already said to do what makes you happy and I'm fine with that. I'll also say that just because someone 20yrs ago said it doesn't add any validity. People 20 years ago were also advocating going spores to grain and suffocating your tubs til fully colonized in full darkness. If I come across the other ones I've seen I'll link them. If not I don't have much else to say besides not 1 TC that currently posts will recommend air dry over dehydrator. It's up to you what you do. And I never said it ruins the mushrooms I'm sure the people you give them too are satisfied so if that's the goal then good job.

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OfflineFunfarm
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Re: Over ripe tissue [Re: WyoMX]
    #28663983 - 02/17/24 01:50 PM (10 months, 24 days ago)

Go Cougs! Maybe your more of a huskies, ducks beavers, or vandals fan? I really wanted to see that youngster at QB beat Michigan. Maybe your none of the above?

Not to berate you, but it was a TC that advocated air drying, and it was a posting along time ago, same thing, not looking it up, anyways I'm sitting here looking at a simple  dehydrator. What do you think of this one? 350 watt, 95 to 165° f



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Offlinefractadactyl
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Re: Over ripe tissue [Re: Funfarm] * 1
    #28664084 - 02/17/24 03:21 PM (10 months, 24 days ago)

I use a box fan laid pointing up on a dolly flat, and stack drying racks made from 2x4s and chicken wire stapled on.
You can put 2 or 3 racks on, and add another fan, more racks.
Stack it up as high as you want, and roll it around where it needs to go.
Big pulls will be dry in a couple days tops.


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OfflineFunfarm
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Re: Over ripe tissue [Re: fractadactyl]
    #28665296 - 02/18/24 10:22 AM (10 months, 23 days ago)

Made 3 rounds of space tea last night, just using a weed grinder, weighed out a .6, 1.5, and 1.7 of grindings.
Allowed the grindings to soak in squeezed lemon juice for about 15 minutes, added milos sweet tea, microwaved until steaming. Excellent movie night.
Air dryed, however the current plates, I have set out, I removed the top plate to allow more air. A friend who I served with came over, he does Asbestos remediation and said he could build a containment area out of construction grade poly and what ever industrial tape they use. His further idea was to cover the basement windows with black poly to not allow light in and it will heat the air as the sunlight passes thru, with the suggestion of a simple space dehumidifier.  Since I really only want to do a tub or 2 at the time, I'm not quite sure but it sounds like a excellent idea.

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OfflineFunfarm
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Re: Over ripe tissue [Re: fractadactyl] * 2
    #28665632 - 02/18/24 02:12 PM (10 months, 23 days ago)

S
Quote:

fractadactyl said:
I use a box fan laid pointing up on a dolly flat, and stack drying racks made from 2x4s and chicken wire stapled on.
You can put 2 or 3 racks on, and add another fan, more racks.
Stack it up as high as you want, and roll it around where it needs to go.
Big pulls will be dry in a couple days tops.




Sipping top shelf whiskey and pounding Budweiser bottles while in orbit rentry from space travels has left me couch island locked for the day. I'm going to Wally's tomorrow for a cheap fan. I thought about it before when I saw the cuisine shop fan blowing their food grade plastic pallets. Ironically a box fan of your description. I'm assuming the air flow should be just over the top of the fruits?

On a side note, if you have Netflix, check out a movie "Don't look up" excellent space coaster movie. Nice and long with funny dialogue, plus Jennifer Lawrence is in it. I find her nice to look at, all though she was made up like a astrophysics Ph.D student in this one and not as pretty. I decided to watch "No hard feelings" after that, again with her, and wow she is pretty in that one. With the last movie "Dune", the actress who plays the chosen ones witch mother, very nice looking also.

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Offlinefractadactyl
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Re: Over ripe tissue [Re: Funfarm]
    #28666311 - 02/19/24 04:15 AM (10 months, 22 days ago)

No, the box fan lays on the dolly flat, airflow going to the ceiling.
Get some 2x4s and cut 4 of them to the same dimension as the box fan. screw them together, now staple chicken wire or window screen onto the 2x4.
Lay that drying rack on top of the fan, so the airflow is blowing the moisture up and off the fruits.
You can make as many racks as you need. I usually stack another boxfan every 3rd drying rack.
Sometimes I'll put it into an old growtent with a space heater if I need stuff dry in a hurry.


--------------------
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