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OfflineYahra
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Re: Ask Quick Questions, Get Quick Answers [Re: LadysKnight] * 1
    #28663549 - 02/17/24 08:57 AM (4 months, 5 days ago)

I was about to post the same.

Recommending cans to melt holes is really a bad thing since they're always too small.

I too used a ravioli can and the holes were so small it was a pita to work with it.

Ended up using a much larger pot lid made of metal to widen the holes.

Here's a pic that shows both diameters:


Edited by Yahra (02/17/24 09:06 AM)

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OfflineSmellyhobbitM
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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra] * 2
    #28663576 - 02/17/24 09:12 AM (4 months, 5 days ago)

So far things that are suspect include SAB armholes, over reliance on perceived sterility (not an inherently bad thing, but in my experience people who rely on “clean rooms” are usually overlooking sterile procedure that actually works), 90 minute grain PC time.

If we keep looking the list will probably grow. You got a lot to o tighten up, which is good news! Much worse to have a total mystery.

Edit: and as frogman Jones pointed out the LC is another vector.


--------------------
A Love Letter to New Growers
A Guide for New Growers
Need Spores? - Sablabs.org

Just because your tub contamed, doesn’t mean your attitude has to contam as well. :mushroom:


Edited by Smellyhobbit (02/17/24 09:34 AM)

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Offlinetree frog
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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 1
    #28663618 - 02/17/24 09:33 AM (4 months, 5 days ago)

LCs!

If a new grower is using LCs...  it's usually the LCs.

Keep in mind it's every culture.  Every jar.  Every tub.

I doubt it's the arm holes getting bumped because of the consistency of the problem.  Though I agree they should be bigger.


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Edited by tree frog (02/17/24 09:34 AM)

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OfflineSmellyhobbitM
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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog]
    #28663625 - 02/17/24 09:35 AM (4 months, 5 days ago)

Yeah true I’m inclined to believe it’s the LC as well.


--------------------
A Love Letter to New Growers
A Guide for New Growers
Need Spores? - Sablabs.org

Just because your tub contamed, doesn’t mean your attitude has to contam as well. :mushroom:


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InvisibleLadysKnight
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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog] * 1
    #28663630 - 02/17/24 09:38 AM (4 months, 5 days ago)

I agree, LCs always suspect, but they did say same result with a2g, so the scope must be wider. It's usually what's growing on the plates.

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OfflineSmellyhobbitM
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Re: Ask Quick Questions, Get Quick Answers [Re: LadysKnight] * 3
    #28663636 - 02/17/24 09:41 AM (4 months, 5 days ago)

Quote:

LadysKnight said:
I agree, LCs always suspect, but they did say same result with a2g, so the scope must be wider. It's usually what's growing on the plates.



Yeah working with LC is as easy as a2a transfers, usually, so if you’re having trouble making good LC then it’s still an inoculant problem. So sterile procedure is still the likely culprit at the end of the chain


--------------------
A Love Letter to New Growers
A Guide for New Growers
Need Spores? - Sablabs.org

Just because your tub contamed, doesn’t mean your attitude has to contam as well. :mushroom:


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Offlinetree frog
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Re: Ask Quick Questions, Get Quick Answers [Re: LadysKnight] * 1
    #28663638 - 02/17/24 09:42 AM (4 months, 5 days ago)

Quote:

LadysKnight said:
I agree, LCs always suspect, but they did say same result with a2g, so the scope must be wider. It's usually what's growing on the plates.




I missed this.  Nice catch.


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OfflineYahra
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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog]
    #28663739 - 02/17/24 10:55 AM (4 months, 5 days ago)

Waiting for the petris to show sign of growth. It's been only 4 days since inoculated so theres plenty of time left.

What should i do in the meantime? Feels like i am wasting time.

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Offlinewifflebb
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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra]
    #28663746 - 02/17/24 10:58 AM (4 months, 5 days ago)

Is this myc piss or mold?


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OfflineSmellyhobbitM
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Re: Ask Quick Questions, Get Quick Answers [Re: wifflebb] * 1
    #28663759 - 02/17/24 11:05 AM (4 months, 5 days ago)

Neither. Your tub is painfully dry


--------------------
A Love Letter to New Growers
A Guide for New Growers
Need Spores? - Sablabs.org

Just because your tub contamed, doesn’t mean your attitude has to contam as well. :mushroom:


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Invisibleelasticaltiger
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Re: Ask Quick Questions, Get Quick Answers [Re: SWBZA]
    #28663762 - 02/17/24 11:06 AM (4 months, 5 days ago)

Quote:

SWBZA said:
No,
Quote:

elasticaltiger said:
Sorry I meant Jar Lids. Was wondering if you had use any cut up SFDs with RTV silicone



Commercial adhesive filters for grain, syringe filters for my LCs. Think I should experiment with syringe filters for grain?




Personally I think RTV creates inconsistent results. If you like having an injection port for grain then yeah I would try that OR just try cracking the lid and squirting.

I don't actually think this is the source of your problem. To confirm, you PCd for 90 minutes?  I think your idea to increase to two hours or more is solid.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

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Offlinewifflebb
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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #28663764 - 02/17/24 11:06 AM (4 months, 5 days ago)

OK good to know - can I dunk the whole thing or should I just keep spraying? Or is it a lost cause?

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OfflineSmellyhobbitM
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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra] * 3
    #28663765 - 02/17/24 11:06 AM (4 months, 5 days ago)

Quote:

Yahra said:
Waiting for the petris to show sign of growth. It's been only 4 days since inoculated so theres plenty of time left.

What should i do in the meantime? Feels like i am wasting time.



In the beginning, starting a new project every week is a good idea. So in a week make more plates and streak more spores if you want. Do that for a couple of months and eventually you will always be a week out from harvest it things go well


--------------------
A Love Letter to New Growers
A Guide for New Growers
Need Spores? - Sablabs.org

Just because your tub contamed, doesn’t mean your attitude has to contam as well. :mushroom:


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OfflineSmellyhobbitM
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Re: Ask Quick Questions, Get Quick Answers [Re: wifflebb]
    #28663778 - 02/17/24 11:15 AM (4 months, 5 days ago)

Quote:

wifflebb said:
OK good to know - can I dunk the whole thing or should I just keep spraying? Or is it a lost cause?



I’d bottom water that and mist the surface for a couple of days. Might even consider sprinkling some fresh coir over the top and letting it grow some new, healthier myc.

In the meantime you need to fix your FAE setup. Right now that is getting way too much air. Are you fanning?


--------------------
A Love Letter to New Growers
A Guide for New Growers
Need Spores? - Sablabs.org

Just because your tub contamed, doesn’t mean your attitude has to contam as well. :mushroom:


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Offlinewifflebb
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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #28663800 - 02/17/24 11:28 AM (4 months, 5 days ago)

Thanks - yeah the problem is that my wife is very nervous about my kids getting into this, so the only allowable space is in the room with our boiler. So it's pretty hot and dry. My other alternative would be bringing it into the garage, but it's pretty cold in there right now (live in NY). So I might just have to talk her into letting me put them in a closet or something.

To bottom water, I'm just lifting up the sides and pour some water underneath? Thanks again.

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Invisibleelasticaltiger
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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 4
    #28663808 - 02/17/24 11:31 AM (4 months, 5 days ago)

Quote:

Smellyhobbit said:
Neither. Your tub is painfully dry




This part doesn't look good to me, a spot of bacteria or mold.



Dry to be sure but also contaminated.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

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InvisibleGleyck
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Re: Ask Quick Questions, Get Quick Answers [Re: elasticaltiger]
    #28663865 - 02/17/24 12:05 PM (4 months, 5 days ago)

what I was about to say... If I had to bet, I would bet on green in a very near future... or on the sides already.
Also, I'm intrigued with the wood sticks.


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teks|pics|notes
--------------------

Edited by Gleyck (02/17/24 12:14 PM)

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InvisibleLemgrub
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Re: Ask Quick Questions, Get Quick Answers [Re: SWBZA]
    #28663887 - 02/17/24 12:24 PM (4 months, 5 days ago)

Quote:

SWBZA said:
:thumbup:




Plz post spawn pics if you got em. Side by side of agar inoculated and LC inoculated would be best. And timeframe for colonization? LC shouldn't be much longer than 10 days.

Do you rinse grains after cooking? Where does your water come from? Try to find a water quality analysis.

Id recommend adding hydrated lime to your grain prep. Grains are full of microbial toxins and are often sprayed with antifungal compounds.


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We getting that pirate bay, alien shishkebab cordycep money

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OfflineMycobeachgirl
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Re: Ask Quick Questions, Get Quick Answers [Re: elasticaltiger]
    #28663895 - 02/17/24 12:30 PM (4 months, 5 days ago)

A little concerned about this tub - P. Natalensis
S2B on 2/5 and it seems to have stalled/slowed down a little
The bottom right looks off to me
I’m away until Monday so I guess I’ll find out then
Any input is appreciated!


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InvisibleSWBZA
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Re: Ask Quick Questions, Get Quick Answers [Re: elasticaltiger]
    #28663901 - 02/17/24 12:34 PM (4 months, 5 days ago)

Wow, thanks for all the though put into looking at my problems.  I really appreciate it.  I feel like I'm hogging the thread a little bit.  But let me hog it some more.

Firstly, yes, the arm holes are much too small.  I've already got on my to-do list to make them bigger.  Maybe this will motivate my lazy ass to do it tomorrow.  I've heard 8" is not a bad size to go for.  Not discounting advice, but I've grown very happy shrooms with that SAB, so not sure that is the sole cause in this particular instance.  I accept it as a possible contributor.

Secondly, yes, I hear all the comments about LCs.  I've posted elsewhere on the Shroomery my sad story in that regard.  Very sad indeed.  Getting LCs just right is hard.  I've had much success, and much more failure with LC.  Had to throw away two thirds of these:



I was so proud of my babies:



But I got bacterial bag after bacterial bag with those SV-10s.  When I eventually emptied them, I could smell that sour stink.  Broke my heart.  The GT syringes worked, though, got solid grows on them.  Eventually tossed the Natalensis as I could not get them to pin - another sad thread on the Shroomery.  But in this case, most of the tubs we're talking about were agar-to-grain.  Same results with the agar grain and the LC grain - mean green.  Not discounting that the LCs could have been the cause of some of those contaminations, but also not convinced it is the major cause.  Then the agar-to-grain tubs should have worked.  They were are dead as their brothers.

Also, not genetics.  I had GTs, Jack Frosts, and Inca Stargazers in that batch.  All equally dead.

While you guys and gals were writing me beautiful love letters here, as a great mechanism to avoid typing a lot (I'm a lazy bastard!), I've been running my process through my head over and over, trying to identify anything that might have changed, and I've found one thing I want to test with you good folk. Besides being lazy, I'm also very jealous, and I've been really envious of those full canopies I see in people's signatures.  A lot of searching convinced me one trick to try is to mix the grain seeds as uniformly as possible throughout the coir. This is what I did: 

I started off shaking the jar like you do when you shake and break.  I aimed at having loose berries, no clumps.

I then poured the loose berries in the bottom of the tote, and covered with coir.  I mixed the lot like I was mixing concrete - meaning with vigour and probably minor violence :rofl:  I'm joking, but maybe not by a lot.  The berries were well distributed amongst the coir, and I probably bullied them more that is reasonable.

But:

1 - When you shake a jar, it takes time to recover.  Maybe I over-did the shaking.  Could this have weakened the myc, making it unable to compete with trich? 

2 - In mixing the coir and grain, knowing myself, I probably over-did it.  I'm thinking that mixing and mixing and mixing would probably rub off any remaining myc still stuck on the surface of the grain.  I'm assuming I mixed in trich floating around in the air and that taking time to "decently" mix the grain and the coir probably took a lot longer than just layering the grain quickly on top of a layer of coir, and covering as quickly with the rest of the coir.  A fast little pancake vs. mixing cake batter, so to speak...  Weak myc mixed in well with trich.  Sounds rather stupid when I write it out like this.  Rather embarrassing, actually.

So, I think I had a lot of "bare / exposed" grain berries with lots of lost, sad little myc filaments distributed in the general coir bulk having it out with mean green.

I don't like exposed berries, so I put a thin layer of coir on top.  I flattened the surface with a tool I made by bending a stainless steel spatula 90 degrees.  I sterilised it with ISO and wiped it with a clean paper towel before stamping down lightly.  I mention this as it was also new in my process.

Before I go write an essay (!!!), could this mistreatment of my spawn be the problem?  In writing all of this out in detail, I think I solved my own problem. Still, feedback would be welcome.

And, how do I get nice, dense canopies???

:windowtantrum:

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