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Invisiblebetterthnbutter
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Condensation in pre-made petri dishes
    #28660923 - 02/15/24 03:17 PM (10 months, 26 days ago)

So I'm new to agar and cultivation  in general.  Anyways I ordered some pre-made petri dishes to get me started until I get more knowledge on making my own.  Last night I used spore syringe in a SAB to inoculate the dishes.  When I finished up the dishes were clear.  I parafilmed the lids place them in an incubation box with temps at 72ยฐ and woke up to all 5 having condensation build up on the lids. Can anyone tell me what I did wrong? Any info would be greatly appreciated. 

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InvisibleOctopusDisco
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Re: Condensation in pre-made petri dishes [Re: betterthnbutter] * 1
    #28660945 - 02/15/24 03:36 PM (10 months, 26 days ago)

Not sure if this is accurate, but it's the story I tell myself:

We're dealing with condensation, so I try to think of other things with condensation to make an analogy. Take, for example, a soda can in a refrigerator. While it's in the refrigerator, there is no condensation, but when you take the can out, condensation forms on the outside of the can.  This is because the thermal difference between the can itself and the environment that it's in.

Now, let's think about petri dishes. When you purchase or make petri dishes that do not have condensation and they are kept in a stable environment with respect to temperature, no condensation forms. However, when there is biological activity in the petri dishes, it creates heat, which warms the interior of the petri relative to the outside environment, creating a thermal difference that results in condensation.

Basically, the inside of the petri dish is akin to the outside of a cold soda can, and you did nothing wrong; a certain level of condensation in plates is something that must be accepted.

Now, it is not entirely that simple, since there are other factors that have an impact such as humidity levels, etc.

Again, I don't have a strong conviction that this is correct, but it's the story I've been rolling with! Someone, please correct me if I'm wrong :smile:

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OfflineThe Tao
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Registered: 09/12/19
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Re: Condensation in pre-made petri dishes [Re: betterthnbutter]
    #28660951 - 02/15/24 03:42 PM (10 months, 26 days ago)

There is a temperature and humidity variation between the inside and outside of the Petri dishes.
What are you calling an incubation box?

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Invisiblebetterthnbutter
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Re: Condensation in pre-made petri dishes [Re: OctopusDisco]
    #28660972 - 02/15/24 03:56 PM (10 months, 26 days ago)

I appreciate the positivity. Many thanks.


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Invisiblebetterthnbutter
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Re: Condensation in pre-made petri dishes [Re: The Tao]
    #28660974 - 02/15/24 03:58 PM (10 months, 26 days ago)

A black tote. With reptile pad under tote
(outside tote). Temperature gauge sensor on floor of tote.


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โ€œLife moves pretty fast. If you don't stop and look around once in a while, you could miss it.โ€

Edited by betterthnbutter (02/15/24 04:06 PM)

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Invisiblebetterthnbutter
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Re: Condensation in pre-made petri dishes [Re: betterthnbutter]
    #28661000 - 02/15/24 04:09 PM (10 months, 26 days ago)

My SAB is in a grow tent I use As a clean room. By the time I was done it was humid in there from body heat and alcohol lamp. Should I leave the door open you think to cut down on heat and humidity.  I was just trying to be as clean as possible.


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โ€œLife moves pretty fast. If you don't stop and look around once in a while, you could miss it.โ€

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OfflineThe Tao
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Re: Condensation in pre-made petri dishes [Re: betterthnbutter]
    #28661044 - 02/15/24 04:41 PM (10 months, 26 days ago)

Youโ€™re
Quote:

betterthnbutter said:
A black tote. With reptile pad under tote
(outside tote). Temperature gauge sensor on floor of tote.



Why use a black tub? Why use a reptile pad?
What tek are you following?

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InvisibleMycolorado
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Re: Condensation in pre-made petri dishes [Re: The Tao] * 1
    #28661048 - 02/15/24 04:45 PM (10 months, 26 days ago)

Condensation is a non-issue.  Ditch the tote and heat.


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Invisiblebetterthnbutter
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Re: Condensation in pre-made petri dishes [Re: Mycolorado]
    #28661130 - 02/15/24 05:43 PM (10 months, 26 days ago)

Appreciate it. Thanks for the tip. :thumbup:


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Invisiblebetterthnbutter
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Re: Condensation in pre-made petri dishes [Re: The Tao]
    #28661671 - 02/16/24 06:44 AM (10 months, 25 days ago)

Mostly because of YouTube.  I'm going grain spawn to monotub. There seems to be no consensus in the mushroom community so I'm just reading,  watching, and hoping for good outcomes.  When I got into growing pot years ago it was much easier to get good information. There was a small learning curve but I never had a crop fail.  I grew in organic soil so they're was also a buffer for mistakes.  This seems less forgiving. So for now I'm trying to listen to more experienced growers and hoing they know what they are talking about.  While also researching different TEK to find out what will work for me.  Any suggestions for a tried and true method is welcome. (Preferably for beginners) This is probably lack of personal experience but I feel like for every one thing I learn there are ten contradictions


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InvisibleLadysKnight
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Re: Condensation in pre-made petri dishes [Re: betterthnbutter] * 2
    #28661851 - 02/16/24 09:10 AM (10 months, 25 days ago)

As said, the heat mat is heating the bottom of the Petri more than the lid. Condensation forms on cooler surfaces.
:cookiemonster:

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Invisibleolmyco
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Registered: 01/08/24
Posts: 179
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Re: Condensation in pre-made petri dishes [Re: betterthnbutter]
    #28661915 - 02/16/24 09:38 AM (10 months, 25 days ago)

No personal experience with petris by myself (yet). But I heard the recommendation in context of condensation to store them upside down.

Does someone with more experience confirm this or think it is a good/bad recommendation?


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Invisiblehellofresh
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Registered: 09/22/23
Posts: 1,243
Re: Condensation in pre-made petri dishes [Re: olmyco]
    #28662379 - 02/16/24 02:49 PM (10 months, 25 days ago)

Quote:

olmyco said:
No personal experience with petris by myself (yet). But I heard the recommendation in context of condensation to store them upside down.





This doesn't cause any issues. The condensation will usually go away on its own. It does help to store them upside down if there is pooling water in the agar surface.

I would avoid storing them on a heating pad. Leave the dishes in the sab and they will clear up.


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Invisiblebetterthnbutter
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Registered: 02/05/24
Posts: 41
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Re: Condensation in pre-made petri dishes [Re: hellofresh]
    #28663109 - 02/16/24 09:15 PM (10 months, 25 days ago)

Quote:

hellofresh said:
Quote:

[
This doesn't cause any issues. The condensation will usually go away on its own. It does help to store them upside down if there is pooling water in the agar surface.

I would avoid storing them on a heating pad. Leave the dishes in the sab and they will clear up.




Much appreciated!


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โ€œLife moves pretty fast. If you don't stop and look around once in a while, you could miss it.โ€

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