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OfflineShredstar
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Registered: 02/15/24
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Last seen: 1 month, 17 days
Lions Mane - Partial Sterilization and Other Questions
    #28660441 - 02/15/24 07:11 AM (4 months, 8 days ago)

I am planning to try growing Lions Mane in 2 x 5 gallon buckets.  I currently have around 10lb rye spawn, and am planning on using fuel pellets and wheat bran as the bulk substrate.  A few questions:
- Would it be possible to sterilize ONLY the wheat bran, potentially with some fuel pellets, and pasteurize the remaining fuel pellets?  This would be to avoid running the PC multiple times with all the bulk sub.
- Noticed that my grain bags are growing invitro. I won't be able to get to this until this weekend, so I mixed the spawn bags up again. Will this be an issue?

Thanks in advance.  I've looked for info on the first question but didn't see anything about doing it this way.

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OfflineRukusS
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Registered: 06/07/11
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Re: Lions Mane - Partial Sterilization and Other Questions [Re: Shredstar]
    #28660570 - 02/15/24 09:38 AM (4 months, 8 days ago)

Are you going to set the buckets outside? If so no real need to sterilize the wood pellets, pasteurization is good.

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OfflineShredstar
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Re: Lions Mane - Partial Sterilization and Other Questions [Re: Rukus]
    #28660697 - 02/15/24 11:44 AM (4 months, 8 days ago)

No, still to cold for outside.  From my understanding its the bran that creates the need for sterilization...so I was thinking I could sterilize the bran and then mix it in with the pasteurized HWFP. If not I would need to run my PC 3-4 times to get enough to fill the buckets.

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OfflineRukusS
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Re: Lions Mane - Partial Sterilization and Other Questions [Re: Shredstar]
    #28660721 - 02/15/24 12:02 PM (4 months, 8 days ago)

Ya I still think pasteurization alone for the wood pellets is fine. Just make sure the grain is fully colonized before transferring.

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Offlinetaku
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Re: Lions Mane - Partial Sterilization and Other Questions [Re: Rukus]
    #28661413 - 02/15/24 09:20 PM (4 months, 8 days ago)

the bran is superfood for microorganisms. so if you pc the bran, it removes microorganisms from it, but when you pitch it into the pasteurized hwfp, then any lingering micros in the hwfp can potentially chow down on the bran and explode in growth. 

its probable that things will go well, but there's a chance you would get contamination.

you can: use more spawn to boost yield, OR, start with small amounts of bran and slowly add more each time you make a bucket until you find a good balance. 


the spawn starting to grow and then being shaken is not an issue. LM is famous for that.


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Offlinewtfcrazymofo
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Registered: 07/26/15
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Re: Lions Mane - Partial Sterilization and Other Questions [Re: Shredstar]
    #28661711 - 02/16/24 07:11 AM (4 months, 7 days ago)

That's easy, get distilled water agar going put 1 grain on some and aborts on others, then make clean growth on more mea agar then make more grain jars, and spawn 1 quart to just plain un-supplemented boiling water pasteurized synthetic logs.  And just spawn that slightly over mature bag of spawn to plain un-supplemented blocks.


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If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922



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