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Invisiblejoze
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Registered: 11/10/20
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Re: Need some agar sectoring suggestions [Re: koma23]
    #28650173 - 02/07/24 10:32 AM (11 months, 3 days ago)

Quote:

koma23 said:
Quote:

joze said:
A single mushroom contains multiple genetically distinct organisms coming together to reproduce. It's not just a male and a female. Your clone still contains all the genetic information of all the organisms that came together to form the mushroom. The reason you clone, is because you are eliminating the other genetics in the tub -- you are only cloning the organisms in that one mushroom.

Let's say you have some spores that you germinate, and you fruit them, and you get one really scrawny phenotype, and one stockier phenotype you really like. The scrawny phenotype and the stocky phenotype are going to be different combinations of the genetics from the spores. The stockier phenotype is a genetic mix that you like, so you clone it, to retain those genetics.





Yeah, I totally get that, tnx. I'm unsure about cloning visa vie sectoring. If I select only specific sectors, the resulting fruit wont be a clone anymore ... or is the resulting fruit still in all likelihood going to be 90% the same?



From my understanding, when you sector, you will lose a small % of the genetics, but if you're only doing three or four transfers before fruiting, it's negligible.

Fungal genetics and reproduction is unfortunately not my strength. Hopefully someone more educated than I can chime in.


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Offlinekoma23
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Registered: 09/15/20
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Last seen: 6 hours, 40 minutes
Re: Need some agar sectoring suggestions [Re: joze]
    #28650422 - 02/07/24 01:36 PM (11 months, 3 days ago)

Quote:

joze said:
Quote:

koma23 said:
Quote:

joze said:
A single mushroom contains multiple genetically distinct organisms coming together to reproduce. It's not just a male and a female. Your clone still contains all the genetic information of all the organisms that came together to form the mushroom. The reason you clone, is because you are eliminating the other genetics in the tub -- you are only cloning the organisms in that one mushroom.

Let's say you have some spores that you germinate, and you fruit them, and you get one really scrawny phenotype, and one stockier phenotype you really like. The scrawny phenotype and the stocky phenotype are going to be different combinations of the genetics from the spores. The stockier phenotype is a genetic mix that you like, so you clone it, to retain those genetics.





Yeah, I totally get that, tnx. I'm unsure about cloning visa vie sectoring. If I select only specific sectors, the resulting fruit wont be a clone anymore ... or is the resulting fruit still in all likelihood going to be 90% the same?



From my understanding, when you sector, you will lose a small % of the genetics, but if you're only doing three or four transfers before fruiting, it's negligible.

Fungal genetics and reproduction is unfortunately not my strength. Hopefully someone more educated than I can chime in.




Alright mate, really appreciate you chiming in!

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Offlinekoma23
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Re: Need some agar sectoring suggestions [Re: koma23] * 1
    #28657850 - 02/13/24 02:18 AM (10 months, 28 days ago)

Fellas, this is how the transfers came out. They all look very fluffy and almost mold like, but surely this can't be mold, right?






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Invisibleelasticaltiger
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Re: Need some agar sectoring suggestions [Re: koma23]
    #28658171 - 02/13/24 09:40 AM (10 months, 28 days ago)

I don't see any obvious mold. You're looking good hollywood!


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Offlinekoma23
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Re: Need some agar sectoring suggestions [Re: elasticaltiger]
    #28658191 - 02/13/24 09:55 AM (10 months, 28 days ago)

Quote:

elasticaltiger said:
I don't see any obvious mold. You're looking good hollywood!




Tnx mate!

So the mycelium had a change of heart and switched completely to tomentose or something similar?

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Invisiblejoze
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Registered: 11/10/20
Posts: 1,056
Loc: PNW
Re: Need some agar sectoring suggestions [Re: koma23]
    #28658530 - 02/13/24 02:38 PM (10 months, 28 days ago)

Changes in environmental conditions can trigger a change in growth. Are you using a different batch of agar, or has the temperature changed recently?


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Offlinekoma23
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Registered: 09/15/20
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Re: Need some agar sectoring suggestions [Re: joze]
    #28659172 - 02/14/24 01:42 AM (10 months, 27 days ago)

Quote:

joze said:
Changes in environmental conditions can trigger a change in growth. Are you using a different batch of agar, or has the temperature changed recently?




Different batch yep, but more or less the same recipe ... Temp is the same.

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