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OfflineChaypotle
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Last seen: 10 months, 27 days
Contam?
    #28657773 - 02/12/24 11:02 PM (10 months, 28 days ago)

So I’m a newbie, I know I have been overthinking for a lot of things.
I just want to know if these tubs look healthy, and if the second tub pictures might be contamination such as cobweb mold.

Edit: I should also probably mention that the first tub has been going for a while compared to the second. The second tub has only been cultivating for about a week, inoculated with GT LC.



[url=https://files.shroomery.org/files/24-007/780405551-01ABC590-64C3-46A8-9134-C6312B36683F.jpg[/url]




Edited by Chaypotle (02/13/24 12:13 AM)

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OfflineDERRAYLD
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Re: Contam? [Re: Chaypotle]
    #28657787 - 02/12/24 11:24 PM (10 months, 28 days ago)

2nd tub is contaminated,  first tub doesn't show anything yet.

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OfflineChaypotle
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Re: Contam? [Re: DERRAYLD]
    #28657803 - 02/13/24 12:06 AM (10 months, 28 days ago)

What is it contaminated with? How can you tell,
Is there anything I can do to help it?

I decided to transfer it to a like a ziploc tub, covered it, and put it into its own area.
I also completely cleaned and sterilized the tub it was in.
As far as my other tub goes, it got a lot of bruising the other day because I had too heavy of a spray bottle for misting, so I mixed up the substrate a bit.. does it look like it’ll be okay? Still produce fruits? Sorry for the many questions I’m just worried about my lil guys.

Edited by Chaypotle (02/13/24 12:40 AM)

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Invisibleelasticaltiger
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Re: Contam? [Re: Chaypotle] * 1
    #28657830 - 02/13/24 01:12 AM (10 months, 28 days ago)

The uncolonized grains are the dead giveaway in the second tub.

I looked over the first one and didn't see anything obvious but it doesn't look... ideal for one week.  Fingers crossed.

Do you have pictures of the jars.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

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OfflineDERRAYLD
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Re: Contam? [Re: elasticaltiger]
    #28657832 - 02/13/24 01:19 AM (10 months, 28 days ago)

You can see the fuzz in the second tub, it isn't the mycelium we want.

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OfflineChaypotle
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Re: Contam? [Re: elasticaltiger]
    #28657834 - 02/13/24 01:38 AM (10 months, 28 days ago)

The first one is more like a month, second one is about a week. I didn’t use grain jars, they were all in one bags, the reason I transferred to a tub is because the substrate wasn’t at field capacity.

The first tub was actually spread a bit because of excessive bruising. Mycelium seems to be connecting pretty well, but as I said I’m a beginner so I’m not too sure.

As for the second tub, think maybe cobweb? Or is it just the uncolonized grain that’s a giveaway?

I’m trying UB tek with a Cambodian MSS I have coming this week, not actual uncle bens but G&G Target brand because less moisture. I’ve also heard of people having way more success with G&G brown rice bags, still quick rice.

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OfflineChaypotle
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Re: Contam? [Re: DERRAYLD]
    #28657835 - 02/13/24 01:39 AM (10 months, 28 days ago)

That’s what I was thinking, plus it shouldn’t be growing over the substrate right? Like that’s a dead giveaway of cobweb, no?

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OfflineHappinessStan
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Re: Contam? [Re: Chaypotle]
    #28657836 - 02/13/24 01:50 AM (10 months, 28 days ago)

Quote:

Chaypotle said:
The first one is more like a month, second one is about a week. I didn’t use grain jars, they were all in one bags, the reason I transferred to a tub is because the substrate wasn’t at field capacity.

The first tub was actually spread a bit because of excessive bruising. Mycelium seems to be connecting pretty well, but as I said I’m a beginner so I’m not too sure.

As for the second tub, think maybe cobweb? Or is it just the uncolonized grain that’s a giveaway?

I’m trying UB tek with a Cambodian MSS I have coming this week, not actual uncle bens but G&G Target brand because less moisture. I’ve also heard of people having way more success with G&G brown rice bags, still quick rice.



UBtek or similar sucks ass. Don't waste your time and spores. Do PFtek instead. No bags of pre cooked rice should ever be shot up with a spore syringe.


--------------------





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OfflineChaypotle
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Re: Contam? [Re: HappinessStan]
    #28657838 - 02/13/24 01:58 AM (10 months, 28 days ago)

Yeah.. I know, only reason I’m doing UBtek is because I’m broke and that’s all I can really afford rn. I’m also going to be attempting to do the Capri Sun LC thing.

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OfflineChaypotle
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Re: Contam? [Re: HappinessStan]
    #28657839 - 02/13/24 01:59 AM (10 months, 28 days ago)

The tub that’s actually doing better was inoculated with a MSS, contaminated tub was with LC. Neither of those tubs were UBtek, I’m just making an attempt as someone who doesn’t have much workroom or money😂

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OfflineHappinessStan
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Re: Contam? [Re: Chaypotle]
    #28657849 - 02/13/24 02:17 AM (10 months, 28 days ago)

Quote:

Chaypotle said:
Yeah.. I know, only reason I’m doing UBtek is because I’m broke and that’s all I can really afford rn. I’m also going to be attempting to do the Capri Sun LC thing.



But UBtek is a waste of money. Do PFtek instead.


--------------------





Edited by HappinessStan (02/13/24 02:17 AM)

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OfflineHappinessStan
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Re: Contam? [Re: Chaypotle]
    #28657854 - 02/13/24 02:21 AM (10 months, 28 days ago)

Quote:

Chaypotle said:
The tub that’s actually doing better was inoculated with a MSS, contaminated tub was with LC. Neither of those tubs were UBtek, I’m just making an attempt as someone who doesn’t have much workroom or money😂



Both tubs are dodgy as hell. The fact you're considering UBtek tells us why you're getting contams.
Msss or lc can both spread contams. This is why we work with agar.


--------------------





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OfflineChaypotle
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Re: Contam? [Re: HappinessStan]
    #28657859 - 02/13/24 02:36 AM (10 months, 28 days ago)

I’m not using uncle bens. I found a subreddit thread with someone using Good&Gather in comparison to UB, only 3/8 for UB colonized nicely without contam, as for G&G he did 10 and 8 were perfect. That’s why I’m going with UBtek, just using a substitute, it’s only about a $1.50 a bag.
I’m okay with trial and error, I’m seeing what works out for me. I only plan on doing .1-.2cc in each rice bag. Do you know if Capri Sun is an okay tek for making LC? I’m going to be getting agar as per caution when I’m able to, as of right now this is all I can do. 
If I had more work space I would for sure do PFtek & alike.

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OfflineDERRAYLD
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Re: Contam? [Re: Chaypotle]
    #28657861 - 02/13/24 02:39 AM (10 months, 28 days ago)

Ub and capri son belong on reddit, we don't waste our time with it here because it's a waste of time.

Make the rice and drink your Capri sun as you eat it, then make some brf pucks.

All of the excuses you're making to justify using the bs is not new to us,  many people here have limited space and money just like you.

Do it right the first time.

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OfflineChaypotle
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Re: Contam? [Re: DERRAYLD]
    #28657869 - 02/13/24 02:58 AM (10 months, 28 days ago)

Hence why I’m asking questions? I have a couple different teks going, I’m just figuring out for myself through trial and error what works for me. This post was about the tubs and contamination, neither of which are UBtek. I’m not saying I’m not going to do PFtek in the future, just as of right now all I have is the minute rice and bulk substrate. I know not to POST about the UBtek here, I know it’s far less than ideal, I know there’s a high contamination rate, it’s just a thing I’m attempting until I can get what I need for PFtek. I appreciate that you guys are so passionate about what works for you guys, but just because it’s what I’m able to do right now doesn’t mean I’m making excuses? It’s just what I can do at the moment. I can’t pull jars and grain out of my ass ya know? I am working on getting what I need for better teks though.

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OfflineChaypotle
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Re: Contam? [Re: DERRAYLD]
    #28657875 - 02/13/24 03:04 AM (10 months, 28 days ago)

If you have any resources where I can get everything I need for PFtek for cheap, it’d be greatly appreciated. I don’t want to do UBtek.. I’m minimizing it, it’s more of just an experiment since everyone has different results. I personally don’t see an issue with experimenting, especially when resources are very limited.
I’ve watched a lot of YouTube videos too with successful UBtek, not a lot of success with actual UB brand, I’m going based off what I’ve seen.

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Invisibleelasticaltiger
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Re: Contam? [Re: Chaypotle]
    #28658150 - 02/13/24 09:21 AM (10 months, 28 days ago)

Capri sun LC?

Juices are pasteurized, not sterilized. Usually through an HTST (high temp short time).

And they aren't exactly packaged in "clean rooms". High care for sure but not 'sterile'.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

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Invisibleelasticaltiger
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Re: Contam? [Re: elasticaltiger]
    #28658152 - 02/13/24 09:24 AM (10 months, 28 days ago)



--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

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InvisibleOctopusDisco
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Re: Contam? [Re: elasticaltiger]
    #28658179 - 02/13/24 09:47 AM (10 months, 28 days ago)

The issue with any tek that involves a bag of rice--regardless of brand--is that the probability of contamination is HIGH. Assuming you have clean culture, I'd reckon it exhibits a contamination rate well above 50%. This means that the grow is more likely to fail than to succeed.

If you do PF tek correctly (i.e. following a tek and not straying from the instructions), and assuming you have clean culture, I'd say the probability of contamination is in the low single digits, implying success is more likely than failure.

To do PF Tek, all you need is one glass jar with a lid (buy something for <$3 from the grocery store that has an appropriate container), some vermiculite (~$7 from Jeff), and BRF (<$10 from Jeff, or buy some whole brown rice and throw it in a blender or food processor to make flour). Assuming you have a pot with a lid, water, and a stove, you're pretty much set with just those items. That's around $20 and will provide enough ingredients for multiple jars. I'd say a still air box, or 'SAB', is very important as well. However, doing PF Tek without a SAB is still SO much more preferable to any tek that involves a bag of rice.

I've also seen Jeff selling pre-made PF tek jars for ~$10. I don't have any experience with these, but the reviews on pre-made things for mycology are not great. You will get more value doing them yourself.

I'm all for the thrill of the ride and experimenting, but there are certain teks (i.e. "bag-of-rice" tek) that have been tried many times before with limited success, so they are not recommended.

Good luck!

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Invisibleelasticaltiger
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Re: Contam? [Re: OctopusDisco] * 1
    #28658183 - 02/13/24 09:50 AM (10 months, 28 days ago)

Chay, some of the criticism may seem harsh. But this crew wants you to grow.

What is your budget?


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

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