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Offlineniall_b73
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liquid culture
    #28655450 - 02/11/24 12:23 PM (10 months, 30 days ago)

Hi all, I have just done a few jars of liquid culture using the following technique:

https://www.shroomery.org/forums/showflat.php/Number/5238137

Hopefully all has went well anyway I'm left with one question the whole in the lid of my jar would I be able to pot a synthetic sterile mesh filter paper sticker over the whole I would that be trouble in case it got wet.
If not what is the best option to do with the whole apart from leaving foil over it



Many thanks,
niall_b73


--------------------
next time i order a pizza i'm gonna ask them to cut it into 6 slices instead of 8, because i was'nt fit for 8 the last time !!


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Offlinenormalperson
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Re: liquid culture [Re: niall_b73]
    #28655632 - 02/11/24 03:11 PM (10 months, 30 days ago)

Spores shot into honey water in open air....i predict 100% failure. LC does not need ge, plug the hole with rtv and start over with a tek that's only 5 or 6 years old (or newer).

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Offlineniall_b73
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Re: liquid culture [Re: normalperson]
    #28655678 - 02/11/24 03:38 PM (10 months, 30 days ago)

Quote:

normalperson said:
Spores shot into honey water in open air....i predict 100% failure. LC does not need ge, plug the hole with rtv and start over with a tek that's only 5 or 6 years old (or newer).




I used a sab


I also done this Tek many years ago without a sab and it worked 100%. No contams and I grew from the culture made.
I actually think I done a post on it, I will see if I did in my history.

Anyway thanks for your input

niall_b73


--------------------
next time i order a pizza i'm gonna ask them to cut it into 6 slices instead of 8, because i was'nt fit for 8 the last time !!


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Offlinenormalperson
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Re: liquid culture [Re: niall_b73]
    #28655720 - 02/11/24 04:10 PM (10 months, 30 days ago)

Quote:

niall_b73 said:
Quote:

normalperson said:
Spores shot into honey water in open air....i predict 100% failure. LC does not need ge, plug the hole with rtv and start over with a tek that's only 5 or 6 years old (or newer).




I used a sab


I also done this Tek many years ago without a sab and it worked 100%. No contams and I grew from the culture made.
I actually think I done a post on it, I will see if I did in my history.

Anyway thanks for your input

niall_b73



Deviating from that 18-year-old tek was a smart move, keep us updated with pics, please.

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OfflineYahra
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Re: liquid culture [Re: normalperson] * 1
    #28655745 - 02/11/24 04:27 PM (10 months, 30 days ago)

Still spores to lc is a bad idea, regardless if you use a SAB or not.

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OfflineHappinessStan
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Re: liquid culture [Re: niall_b73]
    #28655753 - 02/11/24 04:32 PM (10 months, 30 days ago)

Quote:

niall_b73 said:
Hi all, I have just done a few jars of liquid culture using the following technique:

https://www.shroomery.org/forums/showflat.php/Number/5238137

Hopefully all has went well anyway I'm left with one question the whole in the lid of my jar would I be able to pot a synthetic sterile mesh filter paper sticker over the whole I would that be trouble in case it got wet.
If not what is the best option to do with the whole apart from leaving foil over it



Many thanks,
niall_b73



You should probably stop following 18 year old teks.


--------------------





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Offlineniall_b73
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Re: liquid culture [Re: HappinessStan]
    #28655757 - 02/11/24 04:35 PM (10 months, 30 days ago)

[
Quote:

HappinessStan said:
Quote:

niall_b73 said:
Hi all, I have just done a few jars of liquid culture using the following technique:

https://www.shroomery.org/forums/showflat.php/Number/5238137

Hopefully all has went well anyway I'm left with one question the whole in the lid of my jar would I be able to pot a synthetic sterile mesh filter paper sticker over the whole I would that be trouble in case it got wet.
If not what is the best option to do with the whole apart from leaving foil over it



Many thanks,
niall_b73



You should probably stop following 18 year old teks.




Probably but we will see.
If it worked then why would it not work now ?

niall_b73


--------------------
next time i order a pizza i'm gonna ask them to cut it into 6 slices instead of 8, because i was'nt fit for 8 the last time !!


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OfflineYahra
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Re: liquid culture [Re: niall_b73]
    #28655765 - 02/11/24 04:39 PM (10 months, 30 days ago)

Why?

I quote a sentence from this tek:

Quote:

This method is not technically completely sterile, but if you have a clean Spore print/spore syringe, and follow the steps exactly, you should have no problem.




Emphasis mine.

Problem is that there is no such a thing as clean spores. They're inherently dirty.

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Offlineniall_b73
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Re: liquid culture [Re: Yahra]
    #28655772 - 02/11/24 04:43 PM (10 months, 30 days ago)

Quote:

Yahra said:
Why?

I quote a sentence from this tek:

Quote:

This method is not technically completely sterile, but if you have a clean Spore print/spore syringe, and follow the steps exactly, you should have no problem.




Emphasis mine.

Problem is that there is no such a thing as clean spores. They're inherently dirty.




Granted, that said and like I said before I have done this before many years ago and had no problem it worked fine.

I have been away from this for some time and only just getting back at it obviously I have some homework to do to catch up.

Still will be curious to see how this pans out tho

niall_b73


--------------------
next time i order a pizza i'm gonna ask them to cut it into 6 slices instead of 8, because i was'nt fit for 8 the last time !!


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InvisibleItsLinkNotZelda
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Re: liquid culture [Re: niall_b73]
    #28655876 - 02/11/24 06:03 PM (10 months, 30 days ago)

Next time I would use high temp silicon on the hole before sterilizing your jar, to make a self healing injection port.

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OfflineCocaineBuffet
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Re: liquid culture [Re: ItsLinkNotZelda]
    #28656055 - 02/11/24 07:53 PM (10 months, 30 days ago)

As others have said when inoculating LC you (must) use a clean culture and not spores.

Can't blame you if spores have worked before because that eliminates the need for knowing how to use agar. We are just urging you to use methods that are more effective.

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OfflineMushroomMommy
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Re: liquid culture [Re: normalperson] * 1
    #28656220 - 02/11/24 10:14 PM (10 months, 29 days ago)

Quote:

normalperson said:
LC does not need ge




Since when does it not need any GE? Not saying youโ€™re incorrect but can you please link a Tek or evidence to back this up? Iโ€™ve recently moved from modified jars to unmodified media bottles but have been only filling them halfway for GE per BabaYaga


--------------------
ABCD stands for Always Be Collecting Data

โ€œI don't know anything about anything, but I know enough about everything to know that you don't know what the fuck you're talking aboutโ€

Edited by MushroomMommy (02/11/24 10:16 PM)

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OfflineMrJong
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Re: liquid culture [Re: niall_b73] * 1
    #28656260 - 02/11/24 11:15 PM (10 months, 29 days ago)

Quote:

niall_b73 said:
If it worked then why would it not work now ?




If something has a 10% chance of working and you land into that 10% first and second tries, you're going to hate your life when everything fails all of a sudden on your third/fourth/fifth

When aside from that we have time-proven techniques that allow for 99% success rates for not much more effort

You can shoot spores into LC if that's all you care for, we're just trying to make you infinitely more efficient, ultimately it's your call

We moved from those techniques for a reason

Edited by MrJong (02/11/24 11:25 PM)

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Offlineniall_b73
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Re: liquid culture [Re: MrJong]
    #28656764 - 02/12/24 12:48 PM (10 months, 29 days ago)

Ok, so all that said what is the best method for making liquid culture from spores then.

niall_b73


--------------------
next time i order a pizza i'm gonna ask them to cut it into 6 slices instead of 8, because i was'nt fit for 8 the last time !!


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OfflineYahra
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Re: liquid culture [Re: niall_b73] * 1
    #28656766 - 02/12/24 12:49 PM (10 months, 29 days ago)

Look into agar. Agar is used to cleanup spores.

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Offlineniall_b73
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Re: liquid culture [Re: Yahra]
    #28656793 - 02/12/24 01:04 PM (10 months, 29 days ago)

Quote:

Yahra said:
Look into agar. Agar is used to cleanup spores.




Any chance you could point me in the right direction, I have been browsing but can't find a proper instruction for it

Thanks
niall_b73


--------------------
next time i order a pizza i'm gonna ask them to cut it into 6 slices instead of 8, because i was'nt fit for 8 the last time !!


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OfflineYahra
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Re: liquid culture [Re: niall_b73]
    #28656810 - 02/12/24 01:09 PM (10 months, 29 days ago)

I don't do LC since i am a noob.

The first thread on LC which uses agar i could find is this one:

Blenderless Liquid Inoculate

Not sure if there are better teks.

You might want to look into SSLC as well, i heard that's the hottest shit nowadays.

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OfflineRockinRobot
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Re: liquid culture [Re: niall_b73]
    #28656813 - 02/12/24 01:10 PM (10 months, 29 days ago)

Quote:

niall_b73 said:
Ok, so all that said what is the best method for making liquid culture from spores then.

niall_b73




You should never make LC from spores. You make LC from clean agar cultures.

All the links you really need are in the Hitchhikers guide thread but here is a good Agar thread

https://www.shroomery.org/forums/showflat.php/Number/21922023

Once you have CLEAN cultures on agar you can use This link to make your LC

https://www.shroomery.org/forums/showflat.php/Number/24740168

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Offlinenormalperson
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Re: liquid culture [Re: MushroomMommy] * 1
    #28665299 - 02/18/24 10:23 AM (10 months, 23 days ago)

Quote:

MushroomMommy said:
Quote:

normalperson said:
LC does not need ge




Since when does it not need any GE? Not saying youโ€™re incorrect but can you please link a Tek or evidence to back this up? Iโ€™ve recently moved from modified jars to unmodified media bottles but have been only filling them halfway for GE per BabaYaga


i should have been clearer. LC lids do not need a hole for GE nor do they need to be loosened. With only a little headroom,  an unmodified lid tightened down, will keep the LC viable for months or years. a loosened lid or a GE hole will allow the LC to lose moisture over time.

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