Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Kratom Powder for Sale   Kraken Kratom Red Vein Kratom   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Capsules   Original Sensible Seeds Bulk Cannabis Seeds   OlympusMyco.com Olympus Myco Grow Bags   North Spore Injection Grain Bag

Jump to first unread post Pages: 1
OfflineTakeatrip
Stranger
Registered: 09/10/23
Posts: 183
Last seen: 2 hours, 6 minutes
Contamination with drops on agar or loop method
    #28648271 - 02/05/24 10:44 PM (3 months, 9 days ago)

How come when I try putting spores onto agar it often goes an off white colour and doesn’t germinate a thing with some syringes? I even get this with streaking with a loop? You can see where I went with the loop but no growth. I have had this with different vendors too. I do flame sterilise the loop beforehand and shake the syringe and the spores are visible.
I have had some luck using BRF cakes and transferring from them onto agar but even that recently has not worked out.
I am keeping everything as sterile as possible but just not getting much luck and wasting dishes.

Extras: Filter Print Post Top
InvisibleWyoMX
I'm a teapot User Gallery
Registered: 07/06/15
Posts: 2,232
Loc: PNW
Re: Contamination with drops on agar or loop method [Re: Takeatrip] * 1
    #28648276 - 02/05/24 11:00 PM (3 months, 9 days ago)

Whats your agar recipe? If your able to germinate on brf then not getting growth on agar something is up it seems like.

Extras: Filter Print Post Top
OfflineTakeatrip
Stranger
Registered: 09/10/23
Posts: 183
Last seen: 2 hours, 6 minutes
Re: Contamination with drops on agar or loop method [Re: WyoMX]
    #28648953 - 02/06/24 12:32 PM (3 months, 8 days ago)

Quote:

WyoMX said:
Whats your agar recipe? If your able to germinate on brf then not getting growth on agar something is up it seems like.



I buy them in from a company that makes them, they are very good and I am pleased with transfers I have done on them.
They are Malt Extract Agar plates.
I just find it odd that only one strain has been successful when using spores straight to agar, the rest have been unsuccessful.
I have just prepared some more so I will see how those go from a new vendor. I am also doing some BRF cakes as a back up for germination.

Extras: Filter Print Post Top
OfflineHappinessStan
Fungivore
Male User Gallery


Registered: 10/10/12
Posts: 1,762
Loc: Worcester, UK
Last seen: 4 days, 9 hours
Re: Contamination with drops on agar or loop method [Re: Takeatrip] * 1
    #28648970 - 02/06/24 12:42 PM (3 months, 8 days ago)

You can simply make a brf/verm puck in a jar with a gas exchange filter and shoot spore syringes onto that, then pluck clean myc to agar. Really helps with spore syringes. I've always found it hard to get a decent germination with spore syringe to agar due to the excess water. Brf pucks work brilliantly and you can even let them grow out and clone from them.


--------------------





Extras: Filter Print Post Top
Invisiblejoze
Male


Registered: 11/10/20
Posts: 1,056
Loc: PNW
Re: Contamination with drops on agar or loop method [Re: HappinessStan]
    #28649191 - 02/06/24 04:12 PM (3 months, 8 days ago)

When you don't see any fuzzy mycelium, and instead the agar turns a milky white color, that's usually a sign of bacterial contamination.

How are you introducing the spores to the agar? You mentioned a flame-sterilized loop. Are you letting your loop cool down for a few seconds before you wipe up some spores? If it's still red hot you'll just kill the spores you touch.


--------------------


Important Links.

Be kind to others.

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: PhytoExtractum Kratom Powder for Sale   Kraken Kratom Red Vein Kratom   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Capsules   Original Sensible Seeds Bulk Cannabis Seeds   OlympusMyco.com Olympus Myco Grow Bags   North Spore Injection Grain Bag


Similar ThreadsPosterViewsRepliesLast post
* Re: MUSHROOM TRIVIA contaminations with agar/rye darkangel932 646 2 04/21/01 03:47 PM
by darkangel932
* Contamination on agar?? mesq 952 4 04/14/03 12:17 AM
by mesq
* Re: Green mold again! Agar experts help me! Anonymous 7,449 9 12/01/99 10:20 AM
by Anonymous
* agar with malted barley syrup Hongosmeester 7,144 17 10/27/19 10:42 PM
by ABCMORPHS
* Contaminated or germinated syringe?? mesq 1,195 6 04/07/03 01:27 PM
by mesq
* several questions about agar majik_monkey 1,803 4 02/10/03 09:26 AM
by fugu
* Can spores get contaminated? Krayzie 1,172 5 01/13/03 05:18 PM
by DSD
* Contaminated Syringes Loop_Theorist 868 4 10/09/02 03:23 PM
by LSAuser

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
94 topic views. 22 members, 99 guests and 35 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.019 seconds spending 0.007 seconds on 12 queries.