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OfflineRoscoeReturnsS
Crotchety chode man
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Registered: 02/12/18
Posts: 1,763
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Re: Ask Quick Questions, Get Quick Answers [Re: jimineycricket]
    #28647167 - 02/05/24 04:07 AM (3 months, 10 days ago)

Quote:

SWBZA said:
Quote:

RoscoeReturns said:
Quote:

SWBZA said:
QQ: Is the "Dry Malt Extract (“DME”) that can be used as an alternative for liquid malt extract" that you can buy at a brewery supplies outlet good enough for using for our agar and LCs?  Fraction of the price of LME that I can buy at my local mushroom shop.




DME is what you want. People here often say LME meaning Light Malt Extract. Your use of LME to denote liquid malt extract is more correctly how the abbreviation is used in brewing circles. Just get the lightest, or lowest Lovibond dry malt extract you can find.



Okay, I tried searching before asking, but no luck so far. How low must lovibond be to be useful for us? Thanks in advance




Any Lovibond will work to grow mycelium. We prefer low Lovibond, I like 2 or 3, because it will make agar that is close to transparent. The higher the Lovibond, or darker the extract, the darker the agar. It will still grow mycelium just fine, but it may be harder to see what else is growing on it.


Quote:

jimineycricket said:
Is it possible for BRF cakes to dry out because of environmental factors like humidity , heat ,tub in tub incubators etc

Or is a BRF cake a protected ecosystem,  once its been given its moisture content in its creation its set ?

I ask as I am in cold conditions 60F and below so am using a tub in tub aqua heater set up but worried about drying out the cakes




You absolutely can dry out BRF cakes using a heat mat or incubator. Best is to heat the room the cakes are in. Localized heat will cause evaporation at best, and speed up any contamination, if present, at worst.

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OfflineRockinRobot
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Registered: 12/08/22
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Re: Ask Quick Questions, Get Quick Answers [Re: SWBZA]
    #28647198 - 02/05/24 05:16 AM (3 months, 10 days ago)

Quote:

SWBZA said:
QQ: Is the "Dry Malt Extract (“DME”) that can be used as an alternative for liquid malt extract" that you can buy at a brewery supplies outlet good enough for using for our agar and LCs?  Fraction of the price of LME that I can buy at my local mushroom shop.




I think everyone actually uses DME but we all just call it LME. You definitely want lovibond 2 not lovibond 4 though. Lighter is better

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InvisibleSWBZA
Male


Registered: 05/09/23
Posts: 207
Re: Ask Quick Questions, Get Quick Answers [Re: RockinRobot]
    #28647209 - 02/05/24 05:34 AM (3 months, 10 days ago)

Quote:

RockinRobot said:
Quote:

SWBZA said:
QQ: Is the "Dry Malt Extract (“DME”) that can be used as an alternative for liquid malt extract" that you can buy at a brewery supplies outlet good enough for using for our agar and LCs?  Fraction of the price of LME that I can buy at my local mushroom shop.




I think everyone actually uses DME but we all just call it LME. You definitely want lovibond 2 not lovibond 4 though. Lighter is better



The brewer just informed me their DME is lovibond 13. There goes my "cheap" option
:dislike:

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OfflineDERRAYLD
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Re: Ask Quick Questions, Get Quick Answers [Re: SWBZA] * 1
    #28647212 - 02/05/24 05:36 AM (3 months, 10 days ago)

Find another brewer with lighter malt extract.

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OnlineSmellyhobbitM
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Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD]
    #28647290 - 02/05/24 07:40 AM (3 months, 9 days ago)

So I’ve got like triple the amount of tubs in my grow space as usual and the substrate wont stop sweating.

Is this just going to be as issue with that many tubs? Any way to mitigate all the water pooling up around the edges? Having warm pools of water has been causing a lot of bacterial jelly to form, and in some cases it’s been bad enough to stunt growth.

Should I just not stack tubs? Grow less?


--------------------
A Love Letter to New Growers
A Guide for New Growers
Need Spores? - Sablabs.org

Just because your tub contamed, doesn’t mean your attitude has to contam as well. :mushroom:


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OfflineSkropi
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Re: Ask Quick Questions, Get Quick Answers [Re: jimineycricket]
    #28647295 - 02/05/24 07:47 AM (3 months, 9 days ago)

Quote:

jimineycricket said:
Is it possible for BRF cakes to dry out because of environmental factors like humidity , heat ,tub in tub incubators etc

Or is a BRF cake a protected ecosystem,  once its been given its moisture content in its creation its set ?

I ask as I am in cold conditions 60F and below so am using a tub in tub aqua heater set up but worried about drying out the cakes



Yes, they can dry. I discovered this now, on my first grow. My theory is that it would be best to let the cakes in the jars till primordia/pins are formed, so as to preserve the water content, and not having a cake sitting in a FC doing nothing, and just loosing moisture. I may be totally wrong of course, but it seems logical tomme, and I will definitely try it. Some of my cakes never fruited, and I suspect two culprits, one is what I already mentioned, and the second is the... opposite, too much water content when making the cakes, which leads to contamination/stalling. Keep in mind that this is just a theory of a noob, based on observations alone, in my first grow, so I could be totally off.


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Ἄνδρα μοι ἔννεπε, Μοῦσα, πολύτροπον

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OfflineDERRAYLD
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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 4
    #28647309 - 02/05/24 08:06 AM (3 months, 9 days ago)

Consider stacking with an air gap, possibly criss crossing the tubs rather.

Edited by DERRAYLD (02/05/24 10:29 AM)

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Offlinesyri
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Registered: 09/27/23
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Re: Ask Quick Questions, Get Quick Answers [Re: DERRAYLD]
    #28647414 - 02/05/24 09:41 AM (3 months, 9 days ago)

:werd:

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InvisibleMr Piggy
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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 1
    #28647545 - 02/05/24 11:50 AM (3 months, 9 days ago)

Quote:

Smellyhobbit said:
So I’ve got like triple the amount of tubs in my grow space as usual and the substrate wont stop sweating.

Is this just going to be as issue with that many tubs? Any way to mitigate all the water pooling up around the edges? Having warm pools of water has been causing a lot of bacterial jelly to form, and in some cases it’s been bad enough to stunt growth.

Should I just not stack tubs? Grow less?




Perfect excuse to get a good dehumidifier.


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🅃🄴🄰🄼 🄵🄾🄸🄻

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Invisiblemycosispeon
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Registered: 05/17/23
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Loc: PNW
Re: Ask Quick Questions, Get Quick Answers [Re: syri]
    #28647552 - 02/05/24 11:54 AM (3 months, 9 days ago)

QQ in relation to this post...

I did a second "break and shake" on these Mazatapec inoculated rye berries 5 days ago when they were between 75-90% colonized.

This was just before I did it on 1/31


This is today


They look clean to me, no signs of adversity (moisture, metabolites, etc), but are they fully colonized and ready to spawn?

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OfflineYahra
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Re: Ask Quick Questions, Get Quick Answers [Re: mycosispeon]
    #28647803 - 02/05/24 03:48 PM (3 months, 9 days ago)

I am doing agar work soon for the first time and i wonder if it is worth getting an inoculation loop?

I got a scalpel and i think i can scrape spores with it as well?

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Offlineskrontz
rando fello
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Registered: 10/14/23
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #28647830 - 02/05/24 04:02 PM (3 months, 9 days ago)

how can i more successfully harvest this second wave after pulling a lot of mushrooms?

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Invisibleelasticaltiger
Like Tigers in Coitus
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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra] * 1
    #28647832 - 02/05/24 04:03 PM (3 months, 9 days ago)

Quote:

Yahra said:
I am doing agar work soon for the first time and i wonder if it is worth getting an inoculation loop?

I got a scalpel and i think i can scrape spores with it as well?




I got one and I hardly ever used it. After I started growing from prints I just started using pre sterilized swabs. They come in two packs and its like 10$ for 200.

The loop is useful if you're using syringes and streaking. But even then swabs work OK for that.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

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OfflinemorningWood
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Re: Ask Quick Questions, Get Quick Answers [Re: elasticaltiger] * 1
    #28647834 - 02/05/24 04:08 PM (3 months, 9 days ago)

A loop can be made from a bread twister tie! I've made a few but don't really use them


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        You can lead a man to knowledge,
          but you can't make him think.

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OfflineSan Pedro GirlS
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I'm a teapot User Gallery


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Re: Ask Quick Questions, Get Quick Answers [Re: Yahra] * 1
    #28647839 - 02/05/24 04:13 PM (3 months, 9 days ago)

Quote:

Yahra said:
I am doing agar work soon for the first time and i wonder if it is worth getting an inoculation loop?

I got a scalpel and i think i can scrape spores with it as well?



I like nichrome loops more than swabs or a scalpel. But suggest you try them all to learn your own preference.


--------------------

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OfflineDRAKENCL
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Registered: 02/02/23
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Re: Ask Quick Questions, Get Quick Answers [Re: San Pedro Girl]
    #28647911 - 02/05/24 05:44 PM (3 months, 9 days ago)



Do they grow stagnant or do they simply rot? Any opinion?


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OnlineGenesisCorruptedS
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Re: Ask Quick Questions, Get Quick Answers [Re: San Pedro Girl]
    #28647912 - 02/05/24 05:46 PM (3 months, 9 days ago)

Quote:

San Pedro Girl said:
Quote:

Yahra said:
I am doing agar work soon for the first time and i wonder if it is worth getting an inoculation loop?

I got a scalpel and i think i can scrape spores with it as well?



I like nichrome loops more than swabs or a scalpel. But suggest you try them all to learn your own preference.




Personally. I got a sterilizer and the loop fits all the way down to the back of it. So I can get it red hot and it comes out and cools almost instantly. That’s what I really like about it.

I haven’t really had very much opportunity to use it though. I’ve been doing a2a since I got it going.

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OnlineHILLBILLY OUTLAWS
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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #28647917 - 02/05/24 05:47 PM (3 months, 9 days ago)

Quote:

DRAKENCL said:


Do they grow stagnant or do they simply rot? Any opinion?




If you mean the aborts they will most likely be overcome by mycelium if you don’t pick them whith the flush. I’d pick them if you plan on dunking for rehydration.


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TEAM SPREAD THE LOVE!
Smellyhobbit said:
Embarrassment and bashfulness are leeches on your ability to learn.

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InvisibleMr Piggy
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Re: Ask Quick Questions, Get Quick Answers [Re: HILLBILLY OUTLAW] * 1
    #28647939 - 02/05/24 06:09 PM (3 months, 9 days ago)

I literally bend a lil piece of bailing wire into a loop and use that :shrug:


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OfflineSirPsycho
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Re: Ask Quick Questions, Get Quick Answers [Re: Mr Piggy] * 1
    #28647969 - 02/05/24 06:45 PM (3 months, 9 days ago)

Quote:

Mr Piggy said:
I literally bend a lil piece of bailing wire into a loop and use that :shrug:



Proper nichrome loops are so good though. It's like a scalpel vs an x-acto. Yeah, they are pretty much the same thing and yeah they both get the job done but the scalpel and the nichrome loop are just so much nicer to use.


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:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023

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