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thecrazyshroom
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Registered: 07/02/21
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Re: BOD's Easy AF polyfil lid TEK [Re: bodhisatta]
#27952640 - 09/16/22 10:36 AM (1 year, 7 months ago) |
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thanks
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MLPAlucard
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Re: BOD's Easy AF polyfil lid TEK [Re: bodhisatta]
#28249185 - 03/27/23 11:56 AM (1 year, 1 month ago) |
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oh my god thank you for this I've been going nuts trying to figure everything out
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groton
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Re: BOD's Easy AF polyfil lid TEK [Re: MLPAlucard]
#28646032 - 02/04/24 06:46 AM (3 months, 11 days ago) |
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Hi All, total Noob here and I've got an idea of the poly lidding and sterilising but I'm confused by a few details I've not seen mentioned.
Apart from the hole with poly in, are the jar lids sealed tight during the pressure cooking?
What are the foil covers over the lids for? Is this to stop drips getting in the jars through the poly/holes?
The jars will have the substrate, eg grains inside before they are sterilised in the pressure cooker. Does extra water from steam get in and turn them to mush rather than the semi-firm texture that is best?
Once jars are sterile, how do you get the spores/agar into the jar to inoculate the grains?
Thanks all really interesting and some amazing advice and skills.
Edited by groton (02/04/24 06:50 AM)
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Nerex
Strange as angels

Registered: 02/06/22
Posts: 193
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Re: BOD's Easy AF polyfil lid TEK [Re: groton]
#28646147 - 02/04/24 08:34 AM (3 months, 11 days ago) |
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I'm not an expert on the hobby, but I think I can answer to these questions:
You should not put air tight sealed jars in the PC for the sterilization; in this specific case, they should not be air tight sealed anyway due to the poly in the hole: the idea is to give a little air exchange at the colonizing myc after the inoculation of the grains, while preventing contaminants in; BUT it's always better to close the lid "finger tight" (search for it), in order to be sure that it's not sealed. Just remember that the jars should be shook right after you open the PC (when the jars are still hot), so you must close the lid tight before doing that.
Yes, the foil is used to prevent to get the poly wet and, even more important, to not let any water going inside the jars during the sterilization process.
The grain should be hydrated before putting them in the PC; not water from the PC should get in the jars, as said in the fil lid part.
You don't get spores in grain jars (that a crapshoot); you inoculate agar with spores and when you have a clean culture you just drop the agar wedge (or wedges, if you cut it to create more inoculation points) inside the jar in the SAB. If you do everything well, you'll get a fully colonized grain jar.
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WyoMX

Registered: 07/06/15
Posts: 2,232
Loc: PNW
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Re: BOD's Easy AF polyfil lid TEK [Re: Nerex]
#28646249 - 02/04/24 10:37 AM (3 months, 11 days ago) |
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Ya just to add on to above as I've ran these lids almost exclusively, and then when I got plastic lids I did the same to them. I absolutely love them, never had to change the poly and never had a failed jar from a gilter issue. Since they have the polyfill filter you can absolutely tighten them all the way before cooking.
And to inoculate them you would open them in your SAB to put in agar or LC. Some people back in the day would take a needle through the polyfill but I don't really recommend that.
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groton
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Re: BOD's Easy AF polyfil lid TEK [Re: WyoMX]
#28646277 - 02/04/24 10:58 AM (3 months, 11 days ago) |
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Thanks all, really helpful.
I've seen some other optins to polyfill such as using silicone to glue a synthetic filter over the hole. Another one is a self-sealing port which I assume is pierced by a needle then seals up. Anyone used these?
I'm a noob and am still at the finding out stage. I'm loooking at basic equipment and stuff I can buy to get a small batch going in a few weeks.
I have a pressure cooker already and making grain jars seems pretty easy. Next stage is inocculation, so its either spore syringe (which are supposedly sterile) or buying a pre-colonised substrate. Is this just a guaranteed contam failure?
I appreciate the benefits of agar, and like 'no-pour' techniques but I think its slightly more work and equipment.
I'm still reading the tek and its incredible how good some of the science is.
Thanks again. Groton
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groton
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Re: BOD's Easy AF polyfil lid TEK [Re: groton]
#28646287 - 02/04/24 11:05 AM (3 months, 11 days ago) |
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So how do you inocculate agar with spores?
Do you just squirt some spore/water on the surface, or sprinkle dry spores from a spore print? Why is this less likely to contam than a grain jar (which seems an identical process apart from the sterile substrate being different).
Thanks, Gro
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WyoMX

Registered: 07/06/15
Posts: 2,232
Loc: PNW
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Re: BOD's Easy AF polyfil lid TEK [Re: groton] 1
#28646296 - 02/04/24 11:20 AM (3 months, 11 days ago) |
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Quote:
groton said: So how do you inocculate agar with spores?
Do you just squirt some spore/water on the surface, or sprinkle dry spores from a spore print? Why is this less likely to contam than a grain jar (which seems an identical process apart from the sterile substrate being different).
Thanks, Gro
So with agar the whole point is to clean up the mycelium. Mushrooms are grown in open air that has millions of mold spores and crap in the air. So there's always a chance that a mold spore or 20 of them winds up on your spores while still in the mushroom then when you take the spore print it also has the mold spores. So with agar you would take one drop of spore syringe or some spores off a spore print and put it on an agar dish. Then let it grow out and transfer to another agar dish. Generally transfer 3 times or more to ensure that the only thing on your dish is mushroom mycelium. Then you put that on the sterile grain.
If you take a spore print that happens to have a few mold spores and inoculate a dozen jars your more then likely gonna lose most those jars as the mold with bloom very quickly.
Agar is a couple more things you gotta have but mainly only a media bottle, petris, agar, and lme. Should be able to get what you need for not to much extra money. Or if you do prefer pours you don't even need the media bottle really. To me it all depends what your after, do you want to grow a decent amount of shrooms or only enough for you and some buddies every now and then? If you want to grow a lot then your gonna have to jump into agar at some point. If you only want some for you and some buddies you can do pftek very easily and still end up with over an ounce for like $60
As far as the other types of lids they all can work well. These type are actually kinda seen as old school but they always worked for me and I'm a big fan but whatever style you make will more then likely work for ya.
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groton
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Re: BOD's Easy AF polyfil lid TEK [Re: WyoMX]
#28646982 - 02/05/24 12:15 AM (3 months, 10 days ago) |
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Thanks that's really informative and helpful.
So agar let's you reduce risk and cost of contam by giving you a checkable stage before innocculation. Makes sense as good myc and contam is presumably easy to see on agar but by the time it's seen on grain the batch is ruined.
Thanks again. Gro
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groton
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Re: BOD's Easy AF polyfil lid TEK [Re: groton]
#28646984 - 02/05/24 12:16 AM (3 months, 10 days ago) |
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What's LME and pftek?
Thx, Gro
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Baba Yaga
Psychedelic Minion

Registered: 09/13/20
Posts: 4,241
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Re: BOD's Easy AF polyfil lid TEK [Re: groton]
#28647101 - 02/05/24 01:59 AM (3 months, 10 days ago) |
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LME = Light Malt Extract, it's one of the nutrients to use with agar.
Updated PF Tek Guide
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