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OfflinexVadisx
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Re: I’ve got a hunch.. EASY clone crossing [Re: Pnin] * 3
    #28600366 - 12/27/23 11:16 PM (4 months, 18 days ago)

All Maketi:


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Thanks to everyone for sharing what they know :smile:. 165F for 24hrs.

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OfflineNichrome
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Re: I’ve got a hunch.. EASY clone crossing [Re: xVadisx]
    #28600396 - 12/28/23 12:26 AM (4 months, 18 days ago)

Thick!


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OfflinexVadisx
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Re: I’ve got a hunch.. EASY clone crossing [Re: Nichrome]
    #28600550 - 12/28/23 07:00 AM (4 months, 18 days ago)

Maketi would scare me in the bedroom. They're...not small! :lol:


--------------------
Thanks to everyone for sharing what they know :smile:. 165F for 24hrs.

Better Holes Tek (Use a Step Drill Bit)
Don't forget your towel: THE HITCHHIKER'S GUIDE TO THE SHROOMERY

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InvisiblefahtsterM
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Re: I’ve got a hunch.. EASY clone crossing [Re: xVadisx] * 5
    #28601405 - 12/28/23 07:02 PM (4 months, 17 days ago)

Quote:

xVadisx said:
All Maketi:





Very nice!

2nd clone looking a lot like the first.  Could be the same culture cloned twice or very similar phenos.. well have to see what they look like finished up


Ms tub looking albino heavy with the phenos.  Definitely going to swab the larger fruits


1st clone 2nd flush nothing to write home about but should put me near or above the 100g dry mark for the 2qt grow.  Not bad for an f2 ime

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OfflinePnin
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Re: I’ve got a hunch.. EASY clone crossing [Re: fahtster]
    #28601439 - 12/28/23 07:21 PM (4 months, 17 days ago)

Lovely stipes on that last pic.


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Re: I’ve got a hunch.. EASY clone crossing [Re: Pnin] * 8
    #28606542 - 01/01/24 11:26 PM (4 months, 13 days ago)

First flush finished up on a couple more f2 maketi tubs..

Heavy ms (dried down to 89g)
   

2nd clone tub.  Definitely looking different than the other clone when mature.. I really like this one.. colors are great.  All fruits were dense AF in both tubs.
 

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Re: I’ve got a hunch.. EASY clone crossing [Re: fahtster] * 9
    #28617976 - 01/11/24 06:49 PM (4 months, 3 days ago)

Some second flushes of the above maketi f2 tubs 

Ms (didn’t put a ton of effort into this one—heavily neglected)


Did get a cool half and half pigmented and albino spore producing cap


The 2nd clone tub did pretty well on the second flush..
 

Claketi f2 clone tub is starting to knot

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OfflinePnin
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Re: I’ve got a hunch.. EASY clone crossing [Re: fahtster]
    #28618050 - 01/11/24 08:06 PM (4 months, 3 days ago)

Hey Faht are those stipes solid?


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OfflineLogicaL ChaosM
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Re: I’ve got a hunch.. EASY clone crossing [Re: fahtster]
    #28618133 - 01/11/24 09:24 PM (4 months, 3 days ago)

That half/half gill phenotype is so cool!

:asianofapproval:

Its crazy how simple the whole process is. Unreal!

:mindblown:


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OfflineLewDoja
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Re: I’ve got a hunch.. EASY clone crossing [Re: Pnin]
    #28618173 - 01/11/24 10:29 PM (4 months, 3 days ago)

Quote:

Pnin said:
Hey Faht are those stipes solid?




Quote:

fahtster said:

2nd clone tub.  Definitely looking different than the other clone when mature.. I really like this one.. colors are great.  All fruits were dense AF in both tubs.






I'm gonna take wild guess....that they are more SOLID than not.


--------------------
a wise man said:
"Bad drugs tell you, that you want more;
  Good drugs tell you, that you've had enough"



      Trades pending:
if we have any pending trades or you never received anything that you were expecting send a PM with details.  I've had a lot going on, and may have overlooked something as well as USPS snafu's.

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Re: I’ve got a hunch.. EASY clone crossing [Re: LewDoja] * 3
    #28618204 - 01/11/24 11:08 PM (4 months, 3 days ago)

:whathesaid:

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InvisibleOctopusDisco
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Re: I’ve got a hunch.. EASY clone crossing [Re: fahtster] * 3
    #28619041 - 01/12/24 06:23 PM (4 months, 2 days ago)

Thank you, Faht, for documenting your findings here! It's cool to know that it is possible to cross clones with standard equipment.

I'm naive to a lot of the technical details, but I've been thinking about the mechanism behind what's going on here. Could it be possible that the "pulverization" treatment is interfering with the standard clamp connection process during cell division?



The mechanism would be that the shear force lyses the leading dikaryotic cell and clamp branch, leaving a monokaryotic cell behind. This monokaryotic cell then begins replicating and looking for a mate. With only one strain, this doesn't really create anything new, since the new combinations of the 2 monokaryotic cells are the old combinations (putting aside the possibility that the pulverization impacts the genetic components of one nucleus to the extent that it mates with itself, like being super inbred). Two strains, however, have the opportunity to cross during the split seconds that they become 4 monokaryotic cells, resulting in new genetic combinations compared to the parents.

Again, I'm so far away from experienced in mycology that some of the experts here might get a good chuckle reading this. If that's the case, would you please point me in the direction of some good information so that I can learn? I'm real good with reading peer-reviewed studies, even if I'm not technically trained in mycology.

Thanks again, faht! This is awesome and I can't wait to try this out one day :smile:

Edited by OctopusDisco (01/12/24 06:24 PM)

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InvisiblefahtsterM
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Re: I’ve got a hunch.. EASY clone crossing [Re: OctopusDisco] * 5
    #28623601 - 01/16/24 02:56 PM (3 months, 29 days ago)

Seems like the most likely explanation.  The pulverizing is leaving micro pieces of myc with one nuclei of each var and when they recombine with compatible myc, a cross is created.  Maybe :shrug:

Claketi clone looking like.. something lol

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OfflineLogicaL ChaosM
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Re: I’ve got a hunch.. EASY clone crossing [Re: fahtster] * 1
    #28623930 - 01/16/24 07:41 PM (3 months, 29 days ago)

Cool!

Kinda looks like a KSSS mutant "Mario Shroom" grow I saw awhile back.

:popcorn:

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InvisiblefahtsterM
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Re: I’ve got a hunch.. EASY clone crossing [Re: LogicaL Chaos] * 4
    #28625863 - 01/18/24 12:09 PM (3 months, 28 days ago)

Claketi decided to show up for the flush haha

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InvisiblefahtsterM
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Re: I’ve got a hunch.. EASY clone crossing [Re: fahtster] * 5
    #28630014 - 01/21/24 03:27 PM (3 months, 24 days ago)

The claketi turned out better than I was hoping for.  Color is awesome.  They’re not the most solid fruit.. a little hollow through the center but what is there is stout.  Found a couple caps that were dropping but still had the veil attached so I got some nice dark and hopefully as clean as possible swabs.


Getting the chop tonight

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InvisibleMojaveMyc
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Re: I’ve got a hunch.. EASY clone crossing [Re: fahtster] * 1
    #28630024 - 01/21/24 03:34 PM (3 months, 24 days ago)

Oh man, now those are gorgeous

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Re: I’ve got a hunch.. EASY clone crossing (swinger tek adjacent) [Re: fahtster] * 3
    #28630131 - 01/21/24 04:33 PM (3 months, 24 days ago)

i didn't use a blender but i did mix two different LC's together and shook them for about a week. APE X Huautla 


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InvisiblefahtsterM
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Re: I’ve got a hunch.. EASY clone crossing (swinger tek adjacent) [Re: normalperson] * 6
    #28646964 - 02/04/24 11:41 PM (3 months, 10 days ago)

I did a miraculix Qtest on the claketi f2 clone..


And this maketi f2 clone..


Here’s some process pics.  In the acids, filtered and mixed with reagent, incubated


It’s really hard to get a pic that captures the correct colors based on how the instructions tell you to read the results but the maketi is about 18mg/g and the claketi is 16mg/g

For tint comparison, I put both directly against a light source

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OfflineLewDoja
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Re: I’ve got a hunch.. EASY clone crossing (swinger tek adjacent) [Re: fahtster] * 1
    #28646994 - 02/05/24 12:38 AM (3 months, 10 days ago)

Quote:







awesome picture Faht!!


--------------------
a wise man said:
"Bad drugs tell you, that you want more;
  Good drugs tell you, that you've had enough"



      Trades pending:
if we have any pending trades or you never received anything that you were expecting send a PM with details.  I've had a lot going on, and may have overlooked something as well as USPS snafu's.

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