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Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 12,840
Loc: Hole
Last seen: 6 hours, 23 minutes
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Re: Recognizing and dealing with contamination [Re: rumfor69] 1
#28642640 - 02/01/24 06:12 AM (3 months, 14 days ago) |
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I wouldn’t hang onto that tub. There are some molds that can make you sick if they sporulate in your house.
Best to toss that.
-------------------- A Love Letter to New Growers A Guide for New Growers Need Spores? - Sablabs.org Just because your tub contamed, doesn’t mean your attitude has to contam as well.

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rokafogtacsuka
Stranger
Registered: 11/01/23
Posts: 21
Last seen: 2 months, 6 days
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Re: Recognizing and dealing with contamination [Re: rumfor69]
#28642748 - 02/01/24 09:09 AM (3 months, 14 days ago) |
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Quote:
rumfor69 said:
Quote:
rokafogtacsuka said:
Quote:
rumfor69 said:
Quote:
rokafogtacsuka said: Hey there,
Could you please help me if this is trichoderma and if I should throw the tub away or not? Is there any way I can save the rest? Its before the first flush. I have a few other tubs in the room.
Thank you!
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So first you gotta just accept that when contamination happens in any step of this hobby. There is no saving, you cant end contamination, or beat it in anyway. It lives there now. If the sub produces some fruits away from it that aren't rotten then they are safe to dry correctly and consume. Keeping this contaminated tub around will not endanger your future grows, but you should be thinking about disposal soon. Don't get too attached, instead get more attached to trying again. Set a schedule where you create new tries every couple weeks or something(timing always startswith grain prep 😉). Overtime you will be able to assess your abilities, and strengthen your practice with the actions in the processes. I dunno if you're using agar but if you're not then you should definitely check out my links and get into agar. Hey I know...fruits are great. You want real power? Learn agar, master making beautiful cultures as a new hobby that make you just wanna put them to grain.
Thank you mate! I successfully made a few tubs without any issues, so strange to have this with me now, with keeping all the neccessary steps to keep everything as steril as possible. I havent open the tub since putting the grain into the bulk and the holes are also covered with micropore. Anyway, I keep my eyes on it and see if I can have a few healthy fruits. Thank you for your checking!
No part of the spawning to bulk process is clean in anyway. There's millions of mold and bacterial spores attached to micro particles of dust in ur space that get in there during spawning, mixing, through the micropore, and just around the lid gaps. Even very successful growers get tubs that fail here n there. If you can make 5 tubs and one fails and ur not an "expert" then you're doing good. That's a high success percentage. So just toss losses and keep moving forward cause ultimately you'll only get better with practice.
Thank you for your thoughts and for pushing me towards a more positive thinking. Sure you know, but its hard when you take care of sg for months and you fail just before the finish line. I took the box out of the room and put it into a different room. Cleaned the grow room as much as I could. Thanks again!
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rokafogtacsuka
Stranger
Registered: 11/01/23
Posts: 21
Last seen: 2 months, 6 days
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Re: Recognizing and dealing with contamination [Re: Smellyhobbit]
#28642750 - 02/01/24 09:10 AM (3 months, 14 days ago) |
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Quote:
Smellyhobbit said: I wouldn’t hang onto that tub. There are some molds that can make you sick if they sporulate in your house.
Best to toss that.
Got you, but for sure that mold was already in the house thats how it could get into the tub. Thanks for your warning!
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MoosemanArnie
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Registered: 02/28/22
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Re: Recognizing and dealing with contamination [Re: PapayaKing]
#28643469 - 02/01/24 11:49 PM (3 months, 14 days ago) |
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Just looks like bruising to me! Def keep an eye on it
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MoosemanArnie
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Registered: 02/28/22
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Re: Recognizing and dealing with contamination [Re: JurelEnLata]
#28643470 - 02/01/24 11:51 PM (3 months, 14 days ago) |
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no dont think so. nice looking spawn
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MushBiskit
Stranger


Registered: 01/07/24
Posts: 6
Loc: Argentina
Last seen: 3 months, 2 days
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Re: Recognizing and dealing with contamination [Re: Kizzle]
#28646300 - 02/04/24 11:24 AM (3 months, 11 days ago) |
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hi, I'm still trying to recognize healthy mycelium, this are the 2 that i have right now

this is PDA with a drop of lions mane LC, it has 23 days since innoc


and this is cracked corn also 23 days old. it was inoculated with B+ spores
what do you think?
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Munchauzen


Registered: 06/22/11
Posts: 14,346
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Re: Recognizing and dealing with contamination [Re: MushBiskit] 2
#28646393 - 02/04/24 12:54 PM (3 months, 11 days ago) |
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Everything looks fine, but I highly recommend never using corn again, let alone cracked corn. The contents in there are now full of starches released from the cracked corn, which is going to inhibit colonization. Its also not super nutritious compared to other cereal grains.
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MushBiskit
Stranger


Registered: 01/07/24
Posts: 6
Loc: Argentina
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Re: Recognizing and dealing with contamination [Re: Munchauzen]
#28646435 - 02/04/24 01:35 PM (3 months, 11 days ago) |
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thanks!
yeah, a friend told me to use corn, but later i realized that he didnt read too much and just grow mushrooms playing by ear, so i started to read about it and inform my self. also it's expensive, now I'm trying red milo
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DRAKENCL
Stranger

Registered: 02/02/23
Posts: 24
Loc: Chile
Last seen: 2 days, 18 hours
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Re: Recognizing and dealing with contamination [Re: MushBiskit]
#28647925 - 02/05/24 05:58 PM (3 months, 10 days ago) |
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I have doubts about these tubs
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Nichrome
I and I are the roots.


Registered: 12/17/18
Posts: 6,932
Loc: Zone 5
Last seen: 3 hours, 33 minutes
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Re: Recognizing and dealing with contamination [Re: DRAKENCL]
#28648096 - 02/05/24 08:12 PM (3 months, 10 days ago) |
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Quote:
DRAKENCL said: I have doubts about these tubs

Pseudomonas.
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“Drink your tea slowly and reverently, as if it is the axis on which the world earth revolves - slowly, evenly, without rushing toward the future.” ― Thich Nhat Hanh
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DRAKENCL
Stranger

Registered: 02/02/23
Posts: 24
Loc: Chile
Last seen: 2 days, 18 hours
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Re: Recognizing and dealing with contamination [Re: Nichrome]
#28648477 - 02/06/24 05:20 AM (3 months, 9 days ago) |
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thank you very much it has been a headache not to recognize the contamination
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turtle_pizza
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Registered: 02/07/24
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Re: Recognizing and dealing with contamination [Re: DRAKENCL]
#28650015 - 02/07/24 08:25 AM (3 months, 8 days ago) |
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Is there anything savable at this point? It's my first time trying this

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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 2,163
Loc: USA
Last seen: 6 hours, 45 minutes
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Re: Recognizing and dealing with contamination [Re: turtle_pizza]
#28650017 - 02/07/24 08:28 AM (3 months, 8 days ago) |
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You can take it away from your grow area and let the fruits finish maturing. Sometimes they will. Sometimes they won’t. Depends how close the trich is to them. Trich can be kinda like an iceberg. (It’s usually much larger underneath the surface.) Do not open that up in your grow area. Trich can be such a cunt to deal with. Afterwards, clean that tub thoroughly with bleach
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Imatryin
Stranger



Registered: 07/06/21
Posts: 488
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Re: Recognizing and dealing with contamination [Re: SupaThaRipper]
#28651058 - 02/07/24 09:27 PM (3 months, 8 days ago) |
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(X-post from semp thread) What do you think of these plates? I’m trying to not make the same mistake I made with my pan prints. I picked beautiful mold colonies to cultivate. These semps are T1’s from spore print->germ plates.



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Nichrome
I and I are the roots.


Registered: 12/17/18
Posts: 6,932
Loc: Zone 5
Last seen: 3 hours, 33 minutes
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Re: Recognizing and dealing with contamination [Re: Imatryin]
#28651186 - 02/08/24 12:36 AM (3 months, 8 days ago) |
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Looks like tamp but on the whispy side. Could be your media recipe. Could be genetics. I don't see it fighting anything.
Could be something else entirely too. All my tamp germ and early transfer plates have a lot more zones and rings and patterns in them. Colors often too.
How fast did those grow?
Probably clean tamp myc...
--------------------
“Drink your tea slowly and reverently, as if it is the axis on which the world earth revolves - slowly, evenly, without rushing toward the future.” ― Thich Nhat Hanh
Edited by Nichrome (02/08/24 12:37 AM)
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Imatryin
Stranger



Registered: 07/06/21
Posts: 488
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Re: Recognizing and dealing with contamination [Re: Nichrome]
#28651439 - 02/08/24 08:34 AM (3 months, 7 days ago) |
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These Semperviva plates are 3-4 w old. They are in unvented deli cups, 1.4% lme and 2% agar. The cups are straight out of the manufacturer packaging into the FFU flow. Agar recipe is sterilized in a media bottle, cooled to pouring temp and done. Contam rate on plates >14 days after pour varies from 0-5%. Contam rate is gradually decreasing as I improve my work flow.
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Cmp5150
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Re: Recognizing and dealing with contamination [Re: Imatryin]
#28651503 - 02/08/24 09:43 AM (3 months, 7 days ago) |
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Is this contamination or caused by something else?
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Nichrome
I and I are the roots.


Registered: 12/17/18
Posts: 6,932
Loc: Zone 5
Last seen: 3 hours, 33 minutes
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Re: Recognizing and dealing with contamination [Re: Imatryin]
#28652366 - 02/08/24 11:59 PM (3 months, 7 days ago) |
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Oh you said semp not tamp. That looks a lot more like semp myc than tamp myc. My mistake.
Probably just fine.
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Shroobe
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Registered: 02/09/24
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Re: Recognizing and dealing with contamination [Re: Nichrome]
#28652566 - 02/09/24 07:00 AM (3 months, 6 days ago) |
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What do you think? Mold or bruising? (In the middle)
Edited by Shroobe (02/09/24 07:01 AM)
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Yam
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Registered: 02/12/24
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Re: Recognizing and dealing with contamination [Re: Shroobe]
#28656342 - 02/12/24 03:41 AM (3 months, 4 days ago) |
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My first frow. Lion's Mane, not sure if it's fine or maybe i have a contamintaion?
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