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ShroomyDmy
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Registered: 02/04/24
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HELP. No sign of pinheads, even after 15 days :(
#28646049 - 02/04/24 07:12 AM (3 months, 11 days ago) |
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After 15 days my cakes aren't showing any sign of pinning 
First step: - Spore syringe (Mckennaii) Second step: - inoculating it in Jar with clean/sterile Rye seeds and Wheat seeds - closed it with aluminium foil so the fresh air can circulate. Third: - Colonization started after 10 days - Fully colonized only after 4 weeks 5th: - Sterilized Coco Coir and the plastic boxes (also added 50g of gips) - Mixed the colonized jars into the mixture - I never sealed the top of the box, fresh air is circulating ever since - Kept the temperature between 70 and 75 grad for 15 days - Now after 15days i dropped the temperature to 55 and there is still no pinheads showing up..
Can anyone explain what am I doing wrong so far? The process is doing well but really really slow. I'm guessing that I got some bad genetics, since the spore prints were really small.



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AspectOfTheCreator
Mastering the Art of Success



Registered: 12/07/22
Posts: 1,634
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Re: HELP. No sign of pinheads, even after 15 days :( [Re: ShroomyDmy]
#28646067 - 02/04/24 07:41 AM (3 months, 11 days ago) |
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Number 1 doesnt look too hot. Thats a bad sign for the other 2 if they followed the same process. You need agar because if you shoot spores into grain jars theres no guarantees or troubleshooting.
It may be controversial but I personally dont even trust syringes for anything but pf tek. I spent a long time trying to fruit several varities that came from seemingly clean mycelium on agar. None ever pinned or fruited. They were all enmeshed with mold. Start from spore prints and streak some plates if looking to go to bulk.
Edited by AspectOfTheCreator (02/04/24 07:48 AM)
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primordialkraken
merciless


Registered: 08/15/18
Posts: 112
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spores = crapshoot to grains. Try the pf tek with spores -or- go agar route to germ spores/cleanup/then noc grain.
EDIT: what Aspect expressed above me ^^^
Edited by primordialkraken (02/04/24 07:50 AM)
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ShroomyDmy
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Registered: 02/04/24
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Last seen: 3 months, 11 days
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Friend of mine did the same process as I did, with the same spore print family and same substrates (shroom-food) and was successful with one flush so far(10g). He got pins within 5days...
My goal is to at least get one flush and collect some spores to do it again with agar, since I won't be able to get some spore prints in the near future. (my friend didn't collect any prints, since the shrooms were really little)
Sooo, if anyone has any advice for this 3 boxes.. HELP 
Btw, the first box, the orange juice is from the grains/seeds..
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Mwj12977
OLD DAD


Registered: 12/12/23
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Re: HELP. No sign of pinheads, even after 15 days :( [Re: ShroomyDmy]
#28646154 - 02/04/24 08:44 AM (3 months, 11 days ago) |
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Toss tub #1 I see trich in there at the 11 o’clock position.
#2 looks wet, those big water droplets shouldn’t be pooling like that. Your lids look to be the culprit there. Take the lid off and dry it out. Leave it off for a bit and let it breathe. Fanning isn’t really recommended anymore but in this case doing it once to help air out the sub wouldn’t be a terrible idea imo.
#3 has dry patches, I would do a mist on that one.
Dropping the temp to induce fruiting should only be an overnight thing. Any longer will stall the process.
Moving forward follow beefys tub tek and you will/should have success.
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ShroomyDmy
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Registered: 02/04/24
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Re: HELP. No sign of pinheads, even after 15 days :( [Re: Mwj12977]
#28646184 - 02/04/24 09:31 AM (3 months, 11 days ago) |
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Mwj12977 said:
Quote:
Toss tub #1 I see trich in there at the 11 o’clock position.
I already throw one with trich, this one looks clean as of now.. It's less myscelium because I cut out part of the surface, it formed the same liquid that's on the picture (seed juice), was scared it will form mold, but it's just sitting on top and not forming any type of mold (as of now)
Quote:
#2 looks wet, those big water droplets shouldn’t be pooling like that. Your lids look to be the culprit there. Take the lid off and dry it out. Leave it off for a bit and let it breathe. Fanning isn’t really recommended anymore but in this case doing it once to help air out the sub wouldn’t be a terrible idea imo.
I turned the heating mats on and fans. (will keep the temp again around 70-75)
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#3 has dry patches, I would do a mist on that one.
Done.
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Dropping the temp to induce fruiting should only be an overnight thing. Any longer will stall the process. Moving forward follow beefys tub tek and you will/should have success.
Any links to share?
Thanks a loot for helping out!
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Mwj12977
OLD DAD


Registered: 12/12/23
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Re: HELP. No sign of pinheads, even after 15 days :( [Re: ShroomyDmy]
#28646221 - 02/04/24 10:09 AM (3 months, 11 days ago) |
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Ah heating mats…..that’s a problem. Do not use heating mats. Heat the room that they are in. General rule of thumb is if you’re comfortable in the room your myc is happy too. Also, that’s probably why the tubs are inconsistent from one another and causing the excess condensation. What’s happening is you’re evaporating all of the water out of the sub. It’s forming on the sides and top, then dripping back down causing fluctuations in your microsphere. That would explain the matting in tub 1.
Sorry, I’m still figuring out how to work the links on this site. I venture to say that someone with more experience in that dept will follow up for me. Hopefully they will explain it as well and I can post the threads in the future. Use the search engine for now and type in “beefys tub tek” and you will get what you’re looking for. READ EVERYTHING BEEFY HAS TO SAY! I cannot stress this more.
Just out of curiosity, are you letting them get light?
On a positive note you are very close to where you want to be. Lots of people don’t even get myc to grow on their first attempt. You will also get some fruit soon, just need to dial it in a bit more.
The liquid should go away after you make some adjustments. I used to see that a lot in my Martha from excess water. I think it’s kinda like mycelium poop and happens sometimes. Not 100% on that because I don’t deal with contaminates very often but I’ve had it before and got good fruits. Just a FYI
Edited by Mwj12977 (02/04/24 10:14 AM)
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WyoMX

Registered: 07/06/15
Posts: 2,232
Loc: PNW
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Re: HELP. No sign of pinheads, even after 15 days :( [Re: Mwj12977] 1
#28646264 - 02/04/24 10:48 AM (3 months, 11 days ago) |
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That Orange "juice" you guys are seeing isn't from the seeds or mycelium poop. It's metabolites and it's a way the mycelium will try to fight infections from mold/bacteria. Folks use to call it mycelium piss but it's simply not that and is a stupid thing to call it imo. That last part isn't directed at anyone in this thread but it gets perpetuated all the time and grinds my gears.
To me tub 1 is toast already, you can keep it around to see but if you have metabolites that bad before pinning it's not a good sign. The other 2 look OK at this point they still have potential to get you some mushrooms.
And if your using spores and so is your buddy multi spore genetics can vary greatly so don't worry about his timeline. Just focus on your tubs and you'll be alright.
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Mwj12977
OLD DAD


Registered: 12/12/23
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Re: HELP. No sign of pinheads, even after 15 days :( [Re: WyoMX] 1
#28646269 - 02/04/24 10:50 AM (3 months, 11 days ago) |
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Thanks Wyo! I appreciate you!
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ShroomyDmy
Stranger

Registered: 02/04/24
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Last seen: 3 months, 11 days
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Re: HELP. No sign of pinheads, even after 15 days :( [Re: Mwj12977]
#28646274 - 02/04/24 10:57 AM (3 months, 11 days ago) |
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Mwj12977 said:
Quote:
Ah heating mats…..that’s a problem. Do not use heating mats. Heat the room that they are in. General rule of thumb is if you’re comfortable in the room your myc is happy too. Also, that’s probably why the tubs are inconsistent from one another and causing the excess condensation. What’s happening is you’re evaporating all of the water out of the sub. It’s forming on the sides and top, then dripping back down causing fluctuations in your microsphere. That would explain the matting in tub 1.
I'm doing this for the first time and in the basement.. It's really hard to keep the temperature around 70-75 without heating mats (especially overnight). I might improvise and put the heating mats on top of the boxes.
Quote:
Sorry, I’m still figuring out how to work the links on this site. I venture to say that someone with more experience in that dept will follow up for me. Hopefully they will explain it as well and I can post the threads in the future. Use the search engine for now and type in “beefys tub tek” and you will get what you’re looking for. READ EVERYTHING BEEFY HAS TO SAY! I cannot stress this more.
Found it, thanks! (you have a link icon above the text field)
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Just out of curiosity, are you letting them get light?
I started using a white lamp 65k 2 days ago. Switching with 12h - without 12h.
Quote:
On a positive note you are very close to where you want to be. Lots of people don’t even get myc to grow on their first attempt. You will also get some fruit soon, just need to dial it in a bit more.
I used heating mats with the jars, I believe that did the trick!
Quote:
The liquid should go away after you make some adjustments. I used to see that a lot in my Martha from excess water. I think it’s kinda like mycelium poop and happens sometimes. Not 100% on that because I don’t deal with contaminates very often but I’ve had it before and got good fruits. Just a FYI
Will keep the box and have a close look at the here and there.
Thank you again for the informative advices!
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ShroomyDmy
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Registered: 02/04/24
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Re: HELP. No sign of pinheads, even after 15 days :( [Re: WyoMX]
#28646278 - 02/04/24 10:59 AM (3 months, 11 days ago) |
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WyoMX said:
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That Orange "juice" you guys are seeing isn't from the seeds or mycelium poop. It's metabolites and it's a way the mycelium will try to fight infections from mold/bacteria. Folks use to call it mycelium piss but it's simply not that and is a stupid thing to call it imo. That last part isn't directed at anyone in this thread but it gets perpetuated all the time and grinds my gears.
Thank you for the information! I'm on the research rn.
Quote:
To me tub 1 is toast already, you can keep it around to see but if you have metabolites that bad before pinning it's not a good sign. The other 2 look OK at this point they still have potential to get you some mushrooms.
I'll keep and see what happens in the upcoming days. It won't harm me if it sits in the corner.
Quote:
And if your using spores and so is your buddy multi spore genetics can vary greatly so don't worry about his timeline. Just focus on your tubs and you'll be alright.
Any idea for far away I'm from getting any fruits?
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WyoMX

Registered: 07/06/15
Posts: 2,232
Loc: PNW
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Re: HELP. No sign of pinheads, even after 15 days :( [Re: ShroomyDmy]
#28646291 - 02/04/24 11:07 AM (3 months, 11 days ago) |
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It's anyone's guess really how far from fruits, could be you see a pin tomorrow or could be you won't see a pin for 2 more weeks. It's just a crapshoot unfortunately. Just keep those Surface Conditions in the right zone and that's about all you can do at this point.
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Bajazly
Stranger... Than You Think



Registered: 09/02/22
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Re: HELP. No sign of pinheads, even after 15 days :( [Re: WyoMX] 1
#28646402 - 02/04/24 01:14 PM (3 months, 10 days ago) |
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I can't believe all you folks missed the problem, I caught it right off the bat.
OP skipped straight from the 3rd step to the 5th, I thought everyone knew the 4th step was the most important of all
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