Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract   OlympusMyco.com Olympus Myco Grow Bags   Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Mono Tub Substrate   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies

Jump to first unread post Pages: 1 | 2 | Next >  [ show all ]
Offlinescapo
Scientist
I'm a teapot User Gallery


Registered: 11/05/22
Posts: 368
Loc: PNW
Last seen: 1 day, 9 hours
Various Psilocybe Species on Agar * 21
    #28645694 - 02/03/24 08:31 PM (3 months, 11 days ago)

A place to post your progress with different species on agar. It is interesting to see the various types of growth isolated from different cultivators!

Here is a list of some of my species on LME/Peptone agar (with a few on Difco R2A agar as labelled). My recipe is 10g LME, 5g peptone, and 50mg gentamycin per liter distilled water.

Psilocybe alutacea:


Psilocybe baeocystis:



Psilocybe banderillensis:




Psilocybe cyanescens:


Psilocybe caeruleoannulata:


Psilocybe fagicola:




Psilocybe gandalfiana (aff. Hopii):




Psilocybe xgriseocaerulea:


Psilocybe heimii:


Psilocybe hispanica (= fimetaria):


Psilocybe hoogshagenii:



Psilocybe indica:


Psilocybe keralensis (needs confirmation, sect. Zap from India):


Psilocybe liniformans:


Psilocybe muliercula:


Psilocybe neoxalapensis:



Psilocybe ovoideocystidiata:


Psilocybe cf. papuana:



Psilocybe pelliculosa:



Psilocybe semilanceata:


Psilocybe serbica:


Psilocybe cf. subaeruginascens:


Psilocybe yungensis:



Psilocybe zapotecorum:

Extras: Filter Print Post Top
InvisibleBaba Yaga
Psychedelic Minion

Registered: 09/13/20
Posts: 4,241
Loc: On Break Vacation
Trusted Cultivator
Re: Various Psilocybe Species on Agar [Re: scapo] * 1
    #28645717 - 02/03/24 08:50 PM (3 months, 11 days ago)

Great work! This is a nice collection of exotics and a good resource :thumbup:

Extras: Filter Print Post Top
OfflineBazl-Begonias
DooDaMan
Male

Registered: 01/24/24
Posts: 27
Last seen: 1 day, 8 hours
Re: Various Psilocybe Species on Agar [Re: Baba Yaga] * 1
    #28645724 - 02/03/24 08:54 PM (3 months, 11 days ago)

How far off is this guy from being fully colonized would y'all say?


--------------------
If I told ya all that went down it'd burn out both of yo ears

Extras: Filter Print Post Top
OfflineBazl-Begonias
DooDaMan
Male

Registered: 01/24/24
Posts: 27
Last seen: 1 day, 8 hours
Re: Various Psilocybe Species on Agar [Re: Bazl-Begonias] * 1
    #28645727 - 02/03/24 08:55 PM (3 months, 11 days ago)







--------------------
If I told ya all that went down it'd burn out both of yo ears

Extras: Filter Print Post Top
Invisiblekirkeng
Stranger
 Unread Journal

Registered: 02/14/21
Posts: 1,029
Loc: Lost
Trusted Cultivator
Re: Various Psilocybe Species on Agar [Re: Bazl-Begonias] * 12
    #28645752 - 02/03/24 09:25 PM (3 months, 11 days ago)

Going to jump in with some pictures I have now, add more later as things continue to come in and grow out. Thanks for the thread scapo this will be fun and informative, sometimes I’m sitting here thinking everything looks fucked just to realize it’s fine after seeing others. Not to mention it will be fun to see the diversity!

Panaeolus cinctulus, MYA 1.2%


Psilocybe subtropicalis, MEA 1.4%


Psilocybe samuiensis, MEA 1.4%


Sec. Zapotecorum NSW, MYA 1.2%


Psilocybe neoxalapensis MEA, 1.4% then MYA 1.2%



Psilocybe banderillensis, MYA 1.2%



Psilocybe angulospora, MYA 1.2%


Psilocybe papuana, MYA 1.2%


Psilocybe caerulescens, MEA 1.4%


Psilocybe baeocystis, MYA 1.2%


Psilocybe caerulipes, MYA 1.2%

Extras: Filter Print Post Top
Offlinescapo
Scientist
I'm a teapot User Gallery


Registered: 11/05/22
Posts: 368
Loc: PNW
Last seen: 1 day, 9 hours
Re: Various Psilocybe Species on Agar [Re: Bazl-Begonias]
    #28645781 - 02/03/24 09:52 PM (3 months, 11 days ago)

Quote:

Bazl-Begonias said:
How far off is this guy from being fully colonized would y'all say?



Roughly 1/3

Extras: Filter Print Post Top
Offlinescapo
Scientist
I'm a teapot User Gallery


Registered: 11/05/22
Posts: 368
Loc: PNW
Last seen: 1 day, 9 hours
Re: Various Psilocybe Species on Agar [Re: kirkeng] * 2
    #28645783 - 02/03/24 09:53 PM (3 months, 11 days ago)

Excellent growth! I’d love to see a handful of these as they colonize more!

I can already see patterns in certain species as well

Edited by scapo (02/03/24 09:59 PM)

Extras: Filter Print Post Top
Offlinesleepydave
Print Proliferator


Registered: 01/20/23
Posts: 182
Last seen: 1 hour, 12 minutes
Re: Various Psilocybe Species on Agar [Re: kirkeng]
    #28645808 - 02/03/24 10:15 PM (3 months, 11 days ago)

:takingnotes:
Will add to this soon!


--------------------

Message me for free Panaeolus prints if you promise to grow them, print, and disperse free of bias in kind!
"I select to create more color, not less."
"All my spores are belong to you."
"PRESERVE LANDRACES!"

Extras: Filter Print Post Top
Invisiblestubb
Dahg Rastubfari
 User Gallery

Registered: 03/23/19
Posts: 1,382
Loc: Memory Flag
Re: Various Psilocybe Species on Agar [Re: sleepydave] * 5
    #28645843 - 02/03/24 11:09 PM (3 months, 11 days ago)

Psilocybe Tampanensis ATL#7:

Germination


T3 dsotm

Edited by stubb (02/04/24 01:47 PM)

Extras: Filter Print Post Top
OnlineAlexandrDughin
Explorer
Male User Gallery
Registered: 10/30/23
Posts: 103
Loc: Europe
Last seen: 9 minutes, 19 seconds
Re: Various Psilocybe Species on Agar [Re: scapo] * 6
    #28645919 - 02/04/24 03:20 AM (3 months, 11 days ago)

That is an incredible ammount of work you are doing, Scapo !

Semilanceata :

slow growth


medium growth but surrounded with bacteria...


fast good growth but i just took transfers from it :frown:

Extras: Filter Print Post Top
Invisiblethe_chosen_one
On the Darkslide
Male User Gallery

Registered: 09/11/06
Posts: 3,244
Loc: 1984
Trusted Cultivator
OG Cultivator
Re: Various Psilocybe Species on Agar [Re: AlexandrDughin] * 9
    #28646029 - 02/04/24 06:43 AM (3 months, 11 days ago)

Great idea! Here's a few of mine. All full strength MEA. Other recipes to follow.


Psilocybe aff. Hopii (Gandalfiana) T2 day 8


Psilocybe aff. Hopii (Gandalfiana) T2 day 26


Psilocybe Angulospora day 12


Psilocybe Angulospora day 19


Psilocybe Banderillensis day 11.


Psilocybe Banderillensis day 19


Psilocybe Caeruleoannulata Chile day 12


Psilocybe Caeruleoannulata Chile day 19


Psilocybe Heimii day 5


Psilocybe Heimii day 16


Psilocybe Samuiensis SW T1 MS day 5


Psilocybe Samuiensis T1 day 24


Psilocybe Zapotecorum Texolo day 11


Psilocybe Zapotecorum Texolo day 19


UD sect. Zapotecorum India T2 day 5


Undescribed section Zapotecorum India (possible Keralensis) T2 day 18


--------------------
"Luck favors the observant." - Workman


Extras: Filter Print Post Top
Offlinescapo
Scientist
I'm a teapot User Gallery


Registered: 11/05/22
Posts: 368
Loc: PNW
Last seen: 1 day, 9 hours
Re: Various Psilocybe Species on Agar [Re: AlexandrDughin]
    #28646385 - 02/04/24 12:48 PM (3 months, 10 days ago)

Quote:

AlexandrDughin said:
That is an incredible ammount of work you are doing, Scapo !

Semilanceata :

slow growth


medium growth but surrounded with bacteria...


fast good growth but i just took transfers from it :frown:




Thank you!

I like the way that middle plate looks

Extras: Filter Print Post Top
Offlinescapo
Scientist
I'm a teapot User Gallery


Registered: 11/05/22
Posts: 368
Loc: PNW
Last seen: 1 day, 9 hours
Re: Various Psilocybe Species on Agar [Re: the_chosen_one] * 1
    #28646386 - 02/04/24 12:49 PM (3 months, 10 days ago)

Excellent work TCO!

Extras: Filter Print Post Top
Offlinepsilocybist
All is One
Male User Gallery


Registered: 05/22/14
Posts: 536
Loc: Far
Last seen: 8 days, 6 hours
Re: Various Psilocybe Species on Agar [Re: scapo]
    #28646899 - 02/04/24 10:20 PM (3 months, 10 days ago)

Just watching.
BTW, to the OP, isn't 50mg/Liter of Gentamicin way too much while 15mg/L is easily an overkill dose?

Extras: Filter Print Post Top
Offlinescapo
Scientist
I'm a teapot User Gallery


Registered: 11/05/22
Posts: 368
Loc: PNW
Last seen: 1 day, 9 hours
Re: Various Psilocybe Species on Agar [Re: stubb]
    #28648179 - 02/05/24 08:56 PM (3 months, 9 days ago)

Quote:

stubb said:
Psilocybe Tampanensis ATL#7:

Germination


T3 dsotm



Interesting how you're getting segmented pigmentation!

Extras: Filter Print Post Top
Offlinescapo
Scientist
I'm a teapot User Gallery


Registered: 11/05/22
Posts: 368
Loc: PNW
Last seen: 1 day, 9 hours
Re: Various Psilocybe Species on Agar [Re: psilocybist]
    #28648182 - 02/05/24 08:57 PM (3 months, 9 days ago)

Where did you find that 15mg/L is too much?

I have been using 50 no problem so far

Edited by scapo (02/05/24 08:58 PM)

Extras: Filter Print Post Top
OfflinePnin
Riz Gukgak


Registered: 07/18/23
Posts: 486
Last seen: 1 day, 14 hours
Re: Various Psilocybe Species on Agar [Re: scapo] * 2
    #28648204 - 02/05/24 09:13 PM (3 months, 9 days ago)

Is this thread only for exotics?

Psilocybe Natalensis 5g LME / 8g agar / 500ml water





--------------------
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ
>my end of 2023 grow journal<

Edited by Pnin (02/05/24 09:14 PM)

Extras: Filter Print Post Top
Offlinescapo
Scientist
I'm a teapot User Gallery


Registered: 11/05/22
Posts: 368
Loc: PNW
Last seen: 1 day, 9 hours
Re: Various Psilocybe Species on Agar [Re: Pnin]
    #28648216 - 02/05/24 09:22 PM (3 months, 9 days ago)

Anything in the genus Psilocybe, with a strong preference for the non-cubensis species. Looks great!

Extras: Filter Print Post Top
Offlinepsilocybist
All is One
Male User Gallery


Registered: 05/22/14
Posts: 536
Loc: Far
Last seen: 8 days, 6 hours
Re: Various Psilocybe Species on Agar [Re: scapo]
    #28648418 - 02/06/24 03:48 AM (3 months, 9 days ago)

Quote:

scapo said:
Where did you find that 15mg/L is too much?
I have been using 50 no problem so far




Recommended concentration for many common antibiotics are given in many articles.
I'm sure whatever protection the media gains from such high doses, is also gained from even 6mg/L.
More slows down myc growth and recovery (beside weakening it against bacterial competition, later on).

And, to respect the thread title:

some old cube or woodlover pic

Extras: Filter Print Post Top
Offlinescapo
Scientist
I'm a teapot User Gallery


Registered: 11/05/22
Posts: 368
Loc: PNW
Last seen: 1 day, 9 hours
Re: Various Psilocybe Species on Agar [Re: psilocybist]
    #28649625 - 02/06/24 08:43 PM (3 months, 8 days ago)

Interesting! In my experience, I do not think it slows down myc growth much (if at all). Very recently, I had ran out of gentamycin and did not use it in my agar. I thought hey, maybe I don't need it, so I made agar without it for almost 2 months. There are some plates in my comment with agar plates that do not have gentamycin in them and you would not be able to tell if I did not mention it.

I generally saw the same rate of growth from the myc across the board with all the species I'm working with on my recipes with and without gentamycin. I did notice a difference in speed when using different nutrients for sure (LME/Peptone vs R2A agar for example). I ended up getting more gentamycin and started using it again a couple weeks ago because I got spores of some new species and it helps a lot with reducing risk on germination plates from wild spores.

Perhaps it is toxic at a point though, not sure where that point is yet

Edited by scapo (02/07/24 08:31 AM)

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | Next >  [ show all ]

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract   OlympusMyco.com Olympus Myco Grow Bags   Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Mono Tub Substrate   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies


Similar ThreadsPosterViewsRepliesLast post
* NEW WORLD PSILOCYBE SPECIES nachoo 1,445 9 08/27/01 08:19 PM
by Zen Peddler
* CASO and Sabouraud-Glucose Agar: Any good ? JeRiKo1 2,111 5 04/19/04 04:24 AM
by JeRiKo1
* Psilocybe weilii indoors Chief_ 3,898 7 01/14/12 12:09 AM
by dtowntoker
* agar
( 1 2 all )
arabmobster 4,501 21 04/11/03 04:10 PM
by arabmobster
* Agar ingredient question Dave_the_stoner 2,113 7 12/06/05 02:10 AM
by Dave_the_stoner
* Agar Work Myc0Maniac 2,759 14 05/13/05 08:17 AM
by Roadkill
* Which species can be cultivated indoors? trunksan 2,508 11 12/14/04 09:59 AM
by trunksan
* Ebay agar agravating me! i8an8th 1,135 5 01/31/06 10:39 AM
by djsage420

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
820 topic views. 18 members, 98 guests and 44 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.023 seconds spending 0.004 seconds on 14 queries.