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Floret
Unmodify everything


Registered: 01/02/23
Posts: 275
Loc: underneath the water
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Update
2024-02-01 No activity on the mex plates after 11 days, how long does it take to germ? I made two more plates with the last of the print. If there's no activity still then my Mexicana A journey ends here  The cube half pints are coming along ok. I shook them sometime between 2024-01-21 and now. Someone say something if it looks off.
-------------------- LAGM 2024
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Womble
Wombling Free

Registered: 09/12/23
Posts: 149
Last seen: 7 days, 21 hours
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Re: LAGM v. 2.024 [Re: Womble]
#28643579 - 02/02/24 04:34 AM (3 months, 13 days ago) |
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Updated.
Quote:
Womble said:
02/02/2024 Bit of a general update post as to what progress has been made over the last few weeks, in addition to the LAGM project I have another 6 strains I am working with, so I'm just having fun with agar at the moment in general.
MVP: On 7/01 I took transfers from my germ plate and a few weeks later these are the plates (pics taken today):

On the 26/01 the one on the left was selected for T2 transfer, looking at the pics today I feel I should have selected the plate on the right (but I don't know why I feel that way exactly, the culture just looks nicer).

Redboy: Again, on 7/01 I took transfers from my germ plate:

Plate on the left hasn't been touched since but the one on the right showed signs of bacteria but not touching any myc. I selected this plate for transfer and chose a site furthest away from the bacteria for my T2 transfer that I did on 12/01:

The T2 plate didn't show any signs of bacteria and so on 26/01 I did a T3 transfer:

GT: On 10/01 I swabbed another 3 plates and as of today I am still not seeing any germination on them, or the original germ plates from 01/01.
If I get no luck with these I guess I will try hydrating the spores and making a SS and then taking that to agar.
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ReverendMyc
succinct is not my forte

Registered: 03/29/19
Posts: 1,849
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Re: LAGM v. 2.024 [Re: Floret] 2
#28643696 - 02/02/24 08:19 AM (3 months, 13 days ago) |
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Quote:
Floret said: Update
2024-02-01 No activity on the mex plates after 11 days, how long does it take to germ? I made two more plates with the last of the print. If there's no activity still then my Mexicana A journey ends here 
With mex and tamps, the age of the spores seems to have a big impact on how fast they germ if at all. They do not seem to last like cube prints do. My fresh prints have germed as fast or faster than cubes. After a year they took longer. After 18 months, some would never germ again no matter what I tried. But not always. I have a 2021 July print that I just started in late December that germed after 2 weeks or so. Don't give up on those plates and they might surprise you.
-------------------- Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023 Don't Panic   
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EniQma

Registered: 11/28/23
Posts: 679
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I’ve been doing things and not updating my op. Somebody kill me 🤣
-------------------- The wild nature of the ovoids being from the same part of the world as me makes me feel like the relationship isn’t founded upon deception (indoor cultivation of non native species through simulation) and has a healthier symbiotic benefit.
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AndImTheHighOne
Stranger


Registered: 12/06/23
Posts: 64
Last seen: 1 month, 6 days
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Re: LAGM v. 2.024 [Re: EniQma]
#28644540 - 02/02/24 08:25 PM (3 months, 12 days ago) |
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Lol same here, man. Not doing so much, but definitely been meaning to give it an update.
Your ovoid myc looked nice, though, hope that's coming along well.
-------------------- "[Y]ou've already bludgeoned and abused the mycelium so at least do it justice and thoroughly mix it into the substrate " ~ Bigfoot My LAGM 2.024
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mycosispeon
Goofy's bastard child


Registered: 05/17/23
Posts: 185
Loc: PNW
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OP updated
Finally got around to another round of isolations. I'm wondering if it's likely these next ones will be suitable to put to grain.
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mnj
Rad Visuospatial Sketchpad



Registered: 11/23/08
Posts: 679
Loc: Inner Space
Last seen: 3 hours, 1 minute
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Quote:
EniQma said: I’ve been doing things and not updating my op. Somebody kill me 🤣
Quote:
AndImTheHighOne said: Lol same here, man. Not doing so much, but definitely been meaning to give it an update.
Your ovoid myc looked nice, though, hope that's coming along well.

Quote:
mycosispeon said: OP updated
Finally got around to another round of isolations. I'm wondering if it's likely these next ones will be suitable to put to grain.
Those are some great looking transfer selections!
I'm cooking some plates today for T2's tomorrow
-------------------- LAGM 2024
Thank yoU please come again FrienD
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LyleChipperson


Registered: 09/29/23
Posts: 91
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Re: LAGM v. 2.024 [Re: mnj]
#28646414 - 02/04/24 01:22 PM (3 months, 10 days ago) |
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OP updated. Natalensis is giving me the best results so far so I will leave the other strains for later and focus on securing good Nat genetics. Tried out SSLC for the first time, not expecting a lot, but it's something to keep me busy while the transfers grow.
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ReverendMyc
succinct is not my forte

Registered: 03/29/19
Posts: 1,849
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2024-02-05
 Going to grain and bunnells to t3 and mex to t2 tonight.
 10 day old subtrop bell caps ready to spawn and 5 day old subtrop Hindsight about to get first shake.
And pf puck takes the early lead on the GT germination race!
  No germination on the two agar plates, but one of the two pucks is taking off. I know this is an agar focused thread, but... EDIT: Correction, both pucks have germinated. Plates haven't yet.
-------------------- Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023 Don't Panic   
Edited by ReverendMyc (02/05/24 04:24 PM)
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fiddle_head
I´m not the dude, guy



Registered: 08/05/08
Posts: 2,448
Loc: luminiferous ether
Last seen: 27 seconds
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Re: LAGM v. 2.024 [Re: Noncense] 1
#28648357 - 02/06/24 02:04 AM (3 months, 9 days ago) |
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I already busted in late. This post will be my whole journal. I will make other posts but mostly for shit talk. come here for the grow log.
proud to be growing this one out, finally. It has been a long time in waiting. Kodama

some little pins forming on the swab there, you might be aple to see the apergillis under the swab handle.
Some more coming up, including makilla gorilla

I also germ´d some gandalf and FP+. Hereś the Gandalf close-up like the rest.
 
happy growing! remember, take it easy. don´t go crazy.
I plan to swab semperviva and natalensis today. A friend ofm ine claims this culture produces a ¨green nip¨ pheno. We are definitely going to find out for sure, unless I can´t grow shit or function sober well enough to pull off a perfect grow (I just quit smoking and am weaning off my ssris. But hey whatever happens, preserved for posterity!
-------------------- lagm 2024 || lagm 2023 || Performance - 2023 || Things That Are Good ============= If you want to use cups instead of petris, read this sauce 🌿Looking for free spores? My inbox is always open.🐒
The Koru 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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Womble
Wombling Free

Registered: 09/12/23
Posts: 149
Last seen: 7 days, 21 hours
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Quote:
ReverendMyc said:
And pf puck takes the early lead on the GT germination race!
  No germination on the two agar plates, but one of the two pucks is taking off. I know this is an agar focused thread, but... EDIT: Correction, both pucks have germinated. Plates haven't yet.
ReverendMyc are the GT from my print? I cannot get mine to germinate on plates for some reason, tried a softer agar and was planning to create a SS in an attempt to hydrate them. but seems you had luck with BRF pucks, I might have to try that.
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ReverendMyc
succinct is not my forte

Registered: 03/29/19
Posts: 1,849
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Re: LAGM v. 2.024 [Re: Womble]
#28648755 - 02/06/24 09:46 AM (3 months, 9 days ago) |
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Hi Womble. It sure is. They wouldn't cooperate on agar for me either so far. The pucks have become my Swiss army knife of mycology. Handy tool that is useful for lots of things. I have a tek about prepared to publish, but all of the core concepts are in Josex' Poke: No Mercy for Bacteria. Mine just expands on other things that pucks are good for, like germinating reluctant spores.
-------------------- Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023 Don't Panic   
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Womble
Wombling Free

Registered: 09/12/23
Posts: 149
Last seen: 7 days, 21 hours
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Quote:
ReverendMyc said: Hi Womble. It sure is. They wouldn't cooperate on agar for me either so far. The pucks have become my Swiss army knife of mycology. Handy tool that is useful for lots of things. I have a tek about prepared to publish, but all of the core concepts are in Josex' Poke: No Mercy for Bacteria. Mine just expands on other things that pucks are good for, like germinating reluctant spores.
Nice, it's good to hear you got some action from them either way. Strange they are not working on agar for both of us, they'e not that old in the grand scheme of things really. I've been successful with way older spores. Sorry they have caused you hassle.
Will look out for the tek and will give the pucks a whirl when I get round to it.
Good luck with the grow.
Edited by Womble (02/06/24 02:07 PM)
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tholos
Stranger


Registered: 03/06/23
Posts: 560
Last seen: 20 hours, 13 minutes
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Re: LAGM v. 2.024 [Re: Womble]
#28649228 - 02/06/24 04:33 PM (3 months, 8 days ago) |
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I finally got some new spores after starting 9 new varieties in December just a few weeks before LAGM started I will more than likely take swabs from tubs I have fruiting currently and add more variety in the next month or two. Better late than never I suppose
Will update this post w/ pictures and formatting once I streak some plates tonight 
Tholos' LAGM 2024
Psilocybe Cubensis




Update 2/19/24
- We have germ on 2/3 Lucid Gates plates
- I will be inoculating 4 BRF pucks with an APE syringe that has been giving me trouble on agar
Update 2/24/24
- APE BRF cakes have been inoculated
- Took two xfers from the rhizo at 10 o clock to new plates for expansion to master SSLC
Update 3/3/24
- Took a transfer directly from my germ plate to SSLC, my previous transfers from this plate are clean so I feel safe using it to inoculate, I will test it on plates in 5-7 days
- APE germination and growth is happening, now we play the waiting game until we get some fruits, or maybe I will get impatient and transfer some BRF to agar so that I can grow it out and inoculate SSLC
Edited by tholos (03/03/24 06:08 PM)
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Gastronomicus
3-0-G



Registered: 03/31/05
Posts: 9,746
Last seen: 8 days, 17 hours
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Re: LAGM v. 2.024 [Re: tholos]
#28649702 - 02/06/24 09:53 PM (3 months, 8 days ago) |
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Small update, finally got around to making grain jars. WBS. Simmered for 20 minutes then let stand covered for another 20. They're in the PC now and I'll send my good plate tomorrow.
-------------------- Make my Funk the P Funk, I wants to get Funked up
LAGM2024
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r0kovski
Stranger

Registered: 09/24/23
Posts: 55
Last seen: 9 days, 7 hours
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Update: 2/2/24 YUxGM T1 agar inoculated to WBS 1qt jar. Also made backup T2 transfer.
Shakti agar contaminated with bacteria, will see if try to isolate, or start from new swab.
7/2/24 YUxGM jar shaken.
Removing P. Natalensis from competition, looks like post with swab got lost.
Edited by r0kovski (02/07/24 05:34 AM)
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ReverendMyc
succinct is not my forte

Registered: 03/29/19
Posts: 1,849
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Re: LAGM v. 2.024 [Re: r0kovski]
#28650851 - 02/07/24 07:06 PM (3 months, 7 days ago) |
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2024-02-07 Spawned the subtrop bell cap in coir trays in tubs

Sent pan cyan bunnells to t3 and mexicana chicon nindo to t2 and both to grain.
  First time using new 70mm glass petri dishes. I poured them too hot so there is a lot of condensation, but they were not too difficult to handle. Testing these out to see if I can make the switch from plastic plates.
-------------------- Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023 Don't Panic   
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,736
Loc: Gamehenge
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I haven't done a goddamn thing....again...
I'll keep it 100% with you guys..I probably ain't gonna see this LAGM through..I've got toooooo much shit goin on at the moment to contribute to this fuckery..but there's a chance..possibly..
--------------------
I 5318008 NOT a virgin!
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mycosispeon
Goofy's bastard child


Registered: 05/17/23
Posts: 185
Loc: PNW
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Quote:
bigfootscreepyuncl said: I haven't done a goddamn thing....again...
I'll keep it 100% with you guys..I probably ain't gonna see this LAGM through..I've got toooooo much shit goin on at the moment to contribute to this fuckery..but there's a chance..possibly..
Damn that sucks dood, but I get it. This is one hobby that requires a bit of commitment and we can only commit to so many things at a time!
I'll try to succeed with those dynamite spores so you can participate by proxy at least

OP updated
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mnj
Rad Visuospatial Sketchpad



Registered: 11/23/08
Posts: 679
Loc: Inner Space
Last seen: 3 hours, 1 minute
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Take care of business Creepyuncle and we'll be waitin for ya when you can do all the things :mmhmm:
-------------------- LAGM 2024
Thank yoU please come again FrienD
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