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InvisibleSWBZA
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Registered: 05/09/23
Posts: 207
Re: AGAR ENVY! (Anything and All things agar!) [Re: koma23]
    #28642619 - 02/01/24 04:56 AM (3 months, 14 days ago)

Hi.

When do you use normal LME agar, and when LME plus yeast? 2% agar seems common. 1.6% LME also seems not far out. What about yeast? What %?

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OfflineSmellyhobbitM
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Re: AGAR ENVY! (Anything and All things agar!) [Re: SWBZA]
    #28642643 - 02/01/24 06:21 AM (3 months, 14 days ago)

.2%


--------------------
A Love Letter to New Growers
A Guide for New Growers
Need Spores? - Sablabs.org

Just because your tub contamed, doesn’t mean your attitude has to contam as well. :mushroom:


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Offlinetree frog
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Re: AGAR ENVY! (Anything and All things agar!) [Re: GenesisCorrupted] * 1
    #28642646 - 02/01/24 06:24 AM (3 months, 14 days ago)

Quote:

GenesisCorrupted said:
Quote:

tree frog said:
Is it worth the effort to clone a slightly soggy abort if it displayed interesting genetics/possible mutation?

This Tamp abort for example?





Out of curiosity, I would.
But only if it isn’t a burden to you. It would just be something to play with.




Thanks, yeah, I put it on four plates yesterday.  I don't have high hopes.  Even the base was soggy once I pulled it open.

I cloned another fat cluster from earlier in that flush.  These may just be aborts and not mutants from those same genetics.

But the aborts were about four times as thick as regular fruits, which was something!

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InvisibleSWBZA
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit]
    #28642668 - 02/01/24 07:01 AM (3 months, 14 days ago)

Quote:

Smellyhobbit said:
.2%



Thanks. When will I want to add the yeast rather than just use LME on its own? Cheers

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Offlinetree frog
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Re: AGAR ENVY! (Anything and All things agar!) [Re: SWBZA]
    #28642678 - 02/01/24 07:33 AM (3 months, 14 days ago)

It promotes pin and stone formation on agar.  I use it when I'm running early transfers looking for genetics that might produce sclerotia.

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InvisibleSWBZA
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Re: AGAR ENVY! (Anything and All things agar!) [Re: tree frog]
    #28642695 - 02/01/24 07:53 AM (3 months, 14 days ago)

Quote:

tree frog said:
It promotes pin and stone formation on agar.  I use it when I'm running early transfers looking for genetics that might produce sclerotia.



Thank you

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Offlinetholos
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Re: AGAR ENVY! (Anything and All things agar!) [Re: SWBZA]
    #28642764 - 02/01/24 09:36 AM (3 months, 14 days ago)

Quote:

SWBZA said:
Thanks. When will I want to add the yeast rather than just use LME on its own? Cheers




The last time I tried my standard recipe w/o yeast, I'd never experienced such weak and odd looking growth on plates. It is the first batch I haven't used yeast in since I started, and I don't plan on ever running it again w/o the yeast added. I use a pretty standard recipe and add 1.0g of Yeast per 1000mL

16g LME - 20g Agar - 1g Yeast - 1000mL


I've seen plenty of people use 1g of Yeast in 500mL of water but I have no personal experience running that

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InvisibleSWBZA
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Re: AGAR ENVY! (Anything and All things agar!) [Re: tholos]
    #28642772 - 02/01/24 09:49 AM (3 months, 14 days ago)

Quote:

tholos said:
Quote:

SWBZA said:
Thanks. When will I want to add the yeast rather than just use LME on its own? Cheers




The last time I tried my standard recipe w/o yeast, I'd never experienced such weak and odd looking growth on plates. It is the first batch I haven't used yeast in since I started, and I don't plan on ever running it again w/o the yeast added. I use a pretty standard recipe and add 1.0g of Yeast per 1000mL

16g LME - 20g Agar - 1g Yeast - 1000mL


I've seen plenty of people use 1g of Yeast in 500mL of water but I have no personal experience running that




Thank you. I hope your next set of plates are spectacular!

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OfflineSan Pedro GirlS
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Re: AGAR ENVY! (Anything and All things agar!) [Re: SWBZA]
    #28642789 - 02/01/24 10:21 AM (3 months, 14 days ago)

Quote:

SWBZA said:
Hi.

When do you use normal LME agar, and when LME plus yeast? 2% agar seems common. 1.6% LME also seems not far out. What about yeast? What %?



Use MEA unless you’re having trouble determining clean growth from contam. The yeast can help the myc grow more vigorously to make visibility easier, but it won’t change your end result since it’s all the same once it hits grain.


--------------------

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InvisibleSWBZA
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Registered: 05/09/23
Posts: 207
Re: AGAR ENVY! (Anything and All things agar!) [Re: San Pedro Girl]
    #28642883 - 02/01/24 12:30 PM (3 months, 14 days ago)

Quote:

San Pedro Girl said:
Quote:

SWBZA said:
When do you use normal LME agar, and when LME plus yeast?



Use MEA unless you’re having trouble determining clean growth from contam. The yeast can help the myc grow more vigorously to make visibility easier, but it won’t change your end result since it’s all the same once it hits grain.



Thanks. I think I might try it once as I am actually really having trouble with testing LCs - maybe it will help me see the contams better. I'm also experimenting with water agar. Gosh, there is much to learn in this hobby.

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Invisiblearnold582


Registered: 10/30/23
Posts: 89
Loc: Mars
Re: AGAR ENVY! (Anything and All things agar!) [Re: SWBZA]
    #28642998 - 02/01/24 01:59 PM (3 months, 13 days ago)

I tried making agar from rye soak water for the first time and it's wonderfully clear, but it seems like it's relatively nutrient poor. Growth on it has seems much slower than I've had on PDA before.

Is there any agar recipe (or a different process) that would produce clear agar without sediment, using things I already have at home?

  • Various wheat flours
  • Spelt flour
  • White rice flour
  • Corn starch, corn meal
  • Potato flakes
  • Diastatic malt (the baking kind)
  • Yeast (the baking kind)
  • Sugar, honey


I have lot of leftover grain water which I froze, could I enrich that with one of the ingredients above to get a nutritious, but still relatively clear agar?

A while ago I tried filtering out the sediment from PDA using my aeropress but it clogged the filter immediately. :grin:

Edited by arnold582 (02/01/24 02:07 PM)

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InvisibleWyoMX
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Re: AGAR ENVY! (Anything and All things agar!) [Re: arnold582] * 3
    #28643071 - 02/01/24 02:47 PM (3 months, 13 days ago)

Slow growth could mean its actually got too many nutrients. We use low nute agar so the mycelium stretches out looking for food. If there's a ton of food in that spot it won't need to spread out so fast to find food and will have a lot slower growth. When I've seen people make grain water agar they normally cut it with regular water. 50/50 seems normal but ive seen people go 90% regular water 10% grain water, but I've never actually made it myself so just throwing that out there.

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Invisiblearnold582


Registered: 10/30/23
Posts: 89
Loc: Mars
Re: AGAR ENVY! (Anything and All things agar!) [Re: WyoMX] * 1
    #28643153 - 02/01/24 04:11 PM (3 months, 13 days ago)

Quote:

WyoMX said:
Slow growth could mean its actually got too many nutrients. We use low nute agar so the mycelium stretches out looking for food. If there's a ton of food in that spot it won't need to spread out so fast to find food and will have a lot slower growth. When I've seen people make grain water agar they normally cut it with regular water. 50/50 seems normal but ive seen people go 90% regular water 10% grain water, but I've never actually made it myself so just throwing that out there.




Oh, I didn't really find any specific recipes when I decided to try it out, so I just used 100% grain water with 2% agar. I'll experiment, thank you!

edit: I should've looked harder the first time around: https://www.shroomery.org/forums/showflat.php/Number/22090249

Edited by arnold582 (02/01/24 04:15 PM)

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OfflineGenesisCorruptedS
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Re: AGAR ENVY! (Anything and All things agar!) [Re: tree frog] * 1
    #28643194 - 02/01/24 04:53 PM (3 months, 13 days ago)

You never know. I remember hearing a story about somebody who got some mutant blobs of penis envy. It turned into a solid slab of viable mushroom that just filled the whole thing.
Turns out he had a special mutant.
Can’t produce any spores, and it was only clonable.
That guy owns a house now.
:lol:

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OfflineBlopblop
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Re: AGAR ENVY! (Anything and All things agar!) [Re: GenesisCorrupted]
    #28643244 - 02/01/24 05:51 PM (3 months, 13 days ago)

Something funky growing on a T-1 plate. So far the T-2 made from this looks good. Any guesses as to what sort of contamination  I’m looking at? Thanks!

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OfflineCocaineBuffet
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Blopblop] * 3
    #28643391 - 02/01/24 09:12 PM (3 months, 13 days ago)

I am personally someone who doesn't care what kind of contam it is but I would transfer from 5 - 8 o clock on that plate

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OfflineNichrome
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Re: AGAR ENVY! (Anything and All things agar!) [Re: nobutreally] * 2
    #28643403 - 02/01/24 09:25 PM (3 months, 13 days ago)

Quote:

nobutreally said:
Interesting.

I'd have to disagree about it being Rhizopus, all the examples I can find of any varieties of Rhizopus look more "blobby" and don't seem to present with the black dots quite like this. With a little digging it almost certainly appears to be Aspergillus niger, though.

C'est la vie, so she goes. I was hopeful that the black dots were "on top" of decent mushroom culture but it doesn't seem that way.




2'nd that ID. Looks like A. niger to me too.


--------------------


To understand someone you must look up to them.

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OfflineBlopblop
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Re: AGAR ENVY! (Anything and All things agar!) [Re: CocaineBuffet]
    #28643448 - 02/01/24 11:05 PM (3 months, 13 days ago)

Right on Man. Thanks!

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InvisibleLyleChipperson


Registered: 09/29/23
Posts: 91
Re: AGAR ENVY! (Anything and All things agar!) [Re: Blopblop]
    #28643635 - 02/02/24 06:40 AM (3 months, 13 days ago)

Can you guys help me figure out what is going on? I started getting these small spots of contamination, and they are becoming increasingly present in my plates.

I use PDA, I used to add food coloring to my batches but I've removed it the last 2 times I did agar. It made no difference in regards to this contamination.

I only have glass petris and PCing them left them with way too much condensation, so I moved to oven sterilization. Except I don't have an oven so I use my air fryer. I wonder if all the air movement is introducing the contamination, but the last 2 batches were sterilized at 200 c for 90 minutes so nothing should survive that.

My agar is usually sterilized for 45 minutes in the PC after venting, last 2 batches went for 1 hour, contamination is still present. I work with small batches, mostly 10 plates at a time.

I do use an SAB and I wonder if it's my technique. I spray the inside with soapy water, wait 3-5 minutes for the drops to settle, work with gloves and iso on my hands and arms, wear a mask so I don't breathe inside the box. I pay attention not to move too quickly and not to hover my hands over open containers. I have a torch on the outside and flame every implement before using it.

Here are photos of a recent transfer plate. I'm trying out new genetics and they tend to take 3+ days to show visible mycelium, I saw the contaminant growing in some plates before any noticeable mycelium. It doesn't seem to be present in every plate of my last batches, but it is there on most of them. The black spots are flakes from cooling down the scalpel.



Would you consider sending mycelium from such a plate to grain if I cut around the mycelium and leave the areas with the small spots in the plate? In a case where there are only a couple spots so it's easy to see that it's clean around the mycelium.

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InvisibleLadysKnight
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Re: AGAR ENVY! (Anything and All things agar!) [Re: LyleChipperson] * 1
    #28643644 - 02/02/24 06:55 AM (3 months, 13 days ago)

Quote:

LyleChipperson said:
Can you guys help me figure out what is going on? I started getting these small spots of contamination, and they are becoming increasingly present in my plates.

I use PDA, I used to add food coloring to my batches but I've removed it the last 2 times I did agar. It made no difference in regards to this contamination.

I only have glass petris and PCing them left them with way too much condensation, so I moved to oven sterilization. Except I don't have an oven so I use my air fryer. I wonder if all the air movement is introducing the contamination, but the last 2 batches were sterilized at 200 c for 90 minutes so nothing should survive that.

My agar is usually sterilized for 45 minutes in the PC after venting, last 2 batches went for 1 hour, contamination is still present. I work with small batches, mostly 10 plates at a time.

I do use an SAB and I wonder if it's my technique. I spray the inside with soapy water, wait 3-5 minutes for the drops to settle, work with gloves and iso on my hands and arms, wear a mask so I don't breathe inside the box. I pay attention not to move too quickly and not to hover my hands over open containers. I have a torch on the outside and flame every implement before using it.

Here are photos of a recent transfer plate. I'm trying out new genetics and they tend to take 3+ days to show visible mycelium, I saw the contaminant growing in some plates before any noticeable mycelium. It doesn't seem to be present in every plate of my last batches, but it is there on most of them. The black spots are flakes from cooling down the scalpel.



Would you consider sending mycelium from such a plate to grain if I cut around the mycelium and leave the areas with the small spots in the plate? In a case where there are only a couple spots so it's easy to see that it's clean around the mycelium.



Are you left handed?

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