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Offlinerumfor69
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Re: Thread Killers - Shroomerys Most Lethal [Re: rumfor69]
    #28642515 - 02/01/24 12:24 AM (3 months, 14 days ago)

Ur fuckin star trek flow hood i love it


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OnlineGenesisCorruptedS
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Re: Thread Killers - Shroomerys Most Lethal [Re: Nichrome]
    #28642517 - 02/01/24 12:25 AM (3 months, 14 days ago)

That’s what I want. A stable product that I can use at a moments notice that lasts a while. Also speeds up everything.
I’m really looking forward to it.
The goal being to have different strains just suspended in animation in these jars. Maybe even being able to give them out to people or sell them.

Edited by GenesisCorrupted (02/01/24 12:32 AM)

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Offlinerumfor69
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Re: Thread Killers - Shroomerys Most Lethal [Re: GenesisCorrupted] * 1
    #28642519 - 02/01/24 12:30 AM (3 months, 14 days ago)

I keep dishes for 6 months at a time. Everything is always isolate making F4+ generational work so you just keep swabbing and plate streaking and working with fresh clean cultures. Maybe cloning occasionally. I might save something in water cryo tubes but still no reason for LC I find. Ur probably using LC and agar right?


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Re: Thread Killers - Shroomerys Most Lethal [Re: rumfor69]
    #28642524 - 02/01/24 12:41 AM (3 months, 14 days ago)

What I’ve been doing is just taking pieces of my favorite agar. And then putting those onto new plates.

I’m probably on the fourth generation of agar.
I have a couple spore prints and a couple swabs.
But all of my currently workable agar is penis envy atm.

I don’t know what’s wrong with my brf but I haven’t seen anything happen with them.
They got pieces of agar too.
I’m gonna probably do more grain tomorrow.

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Offlinerumfor69
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Re: Thread Killers - Shroomerys Most Lethal [Re: GenesisCorrupted] * 1
    #28642538 - 02/01/24 01:09 AM (3 months, 14 days ago)

I in no way mean offense..but LC isn't for beginning stage learning. LC is for dank experts like Nichrome. He could grow mushies from a contaminated LC in my dead grandmas underwear.

Wrapped dishes stored in a container in the fridge can last a year easily. If you need more time then I dunno what ur doing just personally. If you have clean cultures on agar then why tf risk an extra step of performing actions with LC that can contam things. Toss a clean agar wedge or 20 wedges in jars after you transfer a tiny piece from that dish to a new dish or two to keep it going for another year? Then you take clones to agar, then grow those clones and spore swab and streak those clone grows to isolate further the pheno characteristics you wanna propagate?


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OnlineGenesisCorruptedS
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Re: Thread Killers - Shroomerys Most Lethal [Re: rumfor69]
    #28642559 - 02/01/24 01:44 AM (3 months, 14 days ago)

I have material enough to mess with.
Practice makes perfect after all. So I’m gonna keep trying until I get some going.
I have all of these little plugs that I have for these jars I drilled. all of my jars have a injection port. I have a whole bag of them.
In my mind the best thing for me to do is to figure out how to get the liquid cultures going. I’m not knocking you by any means either.
I just want things to go faster. I wouldn’t mind going up to the bulk in the future.
Being able to make a 5 gallon bucket.
I mean at some point I was going to get those little wood plugs that you use for building furniture. And inoculate those so I could tap them into logs around here.
I have plans on a custom built watergun backpack.
Customize a filter so the mushroom bits don’t get sucked into it.
Inoculate everywhere I go on walks.
Big plans for my future.

Edited by GenesisCorrupted (02/01/24 01:32 PM)

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Re: Thread Killers - Shroomerys Most Lethal [Re: rumfor69]
    #28642560 - 02/01/24 01:46 AM (3 months, 14 days ago)

Think of me like a psilocybin super spreader.
Sometimes I see people that just put out a bunch of fresh bark in their yard.
I want to sneak back there at night and inoculate the whole thing with my backpack.
Especially parks. When they put out that fresh layer. You can walk there at night.
I would just walk there at night with a special goal in mind.

Ya know what I’m saying?
:borfase:

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Offlinerumfor69
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Re: Thread Killers - Shroomerys Most Lethal [Re: GenesisCorrupted] * 1
    #28642564 - 02/01/24 01:53 AM (3 months, 14 days ago)

Ahhh so edible food mushy yeah that's a while different game. I thought we were talking actives. No knocking takin btw. I grow bacteria and mold on a professional level lol
I know ppl claim LC goes faster but my a2g master jars are colonized in under 3 weeks. Then each jar is used to inoculate 24 jars worth of grain in grain bags that only take like 14 days. But ultimately...time means nothing. Because if you constantly have agar dishes going and are just making master jars every week then you just never run out and every is happening constantly like time doesn't exist. There's no waiting, there's only constantly using what's ready so LC for me is just an unnecessary extra risk variable.


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Offlinerumfor69
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Re: Thread Killers - Shroomerys Most Lethal [Re: rumfor69] * 1
    #28642565 - 02/01/24 01:54 AM (3 months, 14 days ago)

Johnny psycho seed?


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Re: Thread Killers - Shroomerys Most Lethal [Re: rumfor69]
    #28642572 - 02/01/24 02:06 AM (3 months, 14 days ago)

That makes sense. If you have it dialed in. You don’t need to do anything else. I do want to do more than I currently can just working with agar.
Also, my plans would not just be for edibles.
But I definitely do want some shiitake logs.

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Offlinerumfor69
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Re: Thread Killers - Shroomerys Most Lethal [Re: GenesisCorrupted] * 1
    #28642578 - 02/01/24 02:20 AM (3 months, 14 days ago)

Propagation is always better than some one banging source. It breeds stability. Think of it like a portfolio, being wide and diverse is what's gonna make shit stable. Lots of dishes always doing transfers, always doing a2g every week year after year is gonna make you an expert through practice. I bet even my homie Nichrome has supposedly transferred Thousands of dishes and a2g 😉


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Offlinehuey.bluey
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Re: Thread Killers - Shroomerys Most Lethal [Re: rumfor69]
    #28642650 - 02/01/24 06:32 AM (3 months, 14 days ago)

The first time I tried lc it turned black. I think it's bc I used corn syrup instead of brf or something

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Re: Thread Killers - Shroomerys Most Lethal [Re: huey.bluey] * 1
    #28642856 - 02/01/24 11:49 AM (3 months, 14 days ago)

🎶 I see an LC and I want it painted black.
No rhizomes anymore I want them to turn black. 🎶


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Offlinetwighead
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Re: Thread Killers - Shroomerys Most Lethal [Re: spirit_shadow]
    #28642900 - 02/01/24 12:48 PM (3 months, 14 days ago)

A gun in each hand and a smile on my face!


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Re: Thread Killers - Shroomerys Most Lethal [Re: twighead]
    #28642905 - 02/01/24 12:53 PM (3 months, 14 days ago)

Madlibs --

A gun in each hand and a smile on my face!

:wonka:

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Re: Thread Killers - Shroomerys Most Lethal [Re: durian_2008] * 1
    #28643280 - 02/01/24 06:29 PM (3 months, 14 days ago)


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Re: Thread Killers - Shroomerys Most Lethal [Re: rumfor69] * 1
    #28643407 - 02/01/24 09:38 PM (3 months, 14 days ago)

Quote:

rumfor69 said:
But I only see LC as an extra step. I'd rather have piles of clean dishes, then a2g, then g2g, then s2b




Maybe one day you will see that lc is cutting out many of the steps in the process you mentioned. It is less steps.  :shrug:


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Re: Thread Killers - Shroomerys Most Lethal [Re: Nichrome]
    #28643409 - 02/01/24 09:42 PM (3 months, 14 days ago)

In my mind.
Acquire LC.
I bring that jar into my sab.
I fill syringes.
Then I inject into grain.
Wait till those are fuzzy.
Mix it with some choir.
Throw that into the tub.
Bing bang boom.
Wait for it to fruit.

What I currently do. Is take a piece of agar put it into a jar of grain. Shake it around a little bit. Then wait.
I had BRF that I wanted to try. But they are not working at all with this method. I bet they would work better with liquid culture. I also feel like it would have to be faster.

What do you do?

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Offlinerumfor69
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Re: Thread Killers - Shroomerys Most Lethal [Re: Nichrome]
    #28643425 - 02/01/24 10:07 PM (3 months, 13 days ago)

Quote:

Nichrome said:
Quote:

rumfor69 said:
But I only see LC as an extra step. I'd rather have piles of clean dishes, then a2g, then g2g, then s2b




Maybe one day you will see that lc is cutting out many of the steps in the process you mentioned. It is less steps.  :shrug:




Ur right it cuts out the g2g step but then adds a step of testing the LC back to agar again so I dunno.


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Re: Thread Killers - Shroomerys Most Lethal [Re: rumfor69]
    #28643435 - 02/01/24 10:34 PM (3 months, 13 days ago)

I went agar to LC. I’m not gonna go LC back to agar.
:lol:

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