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AdderLadder
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bigworm] 1
#28641359 - 01/31/24 04:46 AM (3 months, 15 days ago) |
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Quote:
Bigworm said:
Quote:
AdderLadder said:




It's hard to photograph with the condensation. I've been keeping them in the still air box, wasn't sure where else to put them. They're nutcracker spores if it's any help?
To answer where you went wrong, the plates all look like mold, so I would say the steril work is where you started to mess up. Second would be your grain container of choice. I'm not sure what you got going on there but you really should invest in jars with proper filters or unmodified lids. The container, more then likey, allowed contaminated air into it through gaps around the edge or where your ships are. I also think you just noc'ed it up with mold unknowingly. Good 1 thing that most here sould commend you on, is that you went to agar first. You just need a little help with how to clean your culture up first.
I think you're right on the mold and that it just looked like mycelium because it was quite white at first and I don't/didn't really know what I'm looking for 


I'll attach some photos of their progression, was it always mould or did it develop later in your opinion?
The boxes are just what I found online - it said mushroom grow box, in my head I wanted to replicate what I'd already done - all in one grow bag.
-------------------- Mushroom growing newbie 
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AspectOfTheCreator
Mastering the Art of Success



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Re: AGAR ENVY! (Anything and All things agar!) [Re: AdderLadder]
#28641430 - 01/31/24 06:46 AM (3 months, 15 days ago) |
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Spend more time reading the threads and teks on here. Mycelium should be symetrical amd consistant looking. Look at others pics. The stuff you have grains in is what youre meant to spawn to once you have a fully colonized jar/bag. Get yourself 2 dozen quart mason jars for grain.
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Bigworm



Registered: 03/19/22
Posts: 2,142
Loc: Murica
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Re: AGAR ENVY! (Anything and All things agar!) [Re: AdderLadder]
#28641483 - 01/31/24 07:29 AM (3 months, 15 days ago) |
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Where did you get your spores from? No vendor names just a general thing like online or a friend, a trusted vendor, ect..
Edited by Bigworm (01/31/24 07:30 AM)
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mycosispeon
Goofy's bastard child


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bigworm]
#28641730 - 01/31/24 10:32 AM (3 months, 15 days ago) |
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Also... store those agar plates upside down and that condensation should go away
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nobutreally
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Re: AGAR ENVY! (Anything and All things agar!) [Re: mycosispeon]
#28642038 - 01/31/24 03:25 PM (3 months, 15 days ago) |
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Cleared up the condensate on the garbage plate through the careful but probably ill-advised use of a pop torch.
Is it worth trying to pull some transfers from this or is this all ick and nothing but ick?
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GenesisCorrupted
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Re: AGAR ENVY! (Anything and All things agar!) [Re: nobutreally]
#28642040 - 01/31/24 03:27 PM (3 months, 15 days ago) |
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I would not try to pull anything off of that. It’s all Rhiz
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tree frog
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Is it worth the effort to clone a slightly soggy abort if it displayed interesting genetics/possible mutation?
This Tamp abort for example?
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chris77
Archaic Revivalist



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Re: AGAR ENVY! (Anything and All things agar!) [Re: tree frog]
#28642062 - 01/31/24 03:47 PM (3 months, 15 days ago) |
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crosspost from aqqgqa thread maybe someone on here with an idea and/or experienced with snape myc
Quote:
chris77 said: did i transfer mold? or was there just bacteria present, explaining the ‚sludge’ and mycelium is outgrowing it? Starry night

no takers? whatever the gross part is, could the myc be ok? will transfer it anyway, but i do have a doubt if this is cube myc after all…
-------------------- the observer is the observed j. krishnamurti
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nobutreally
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Quote:
GenesisCorrupted said: I would not try to pull anything off of that. It’s all Rhiz
"Rhiz" being what?
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GenesisCorrupted
Taoist, Writer, Student, Artist




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Re: AGAR ENVY! (Anything and All things agar!) [Re: nobutreally]
#28642085 - 01/31/24 04:22 PM (3 months, 15 days ago) |
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Rhizopus Take a look at this. I referred to it quite a bit while working with my agar at the beginning.
It could be mucor. But I don’t see it as much, because that typically comes from the soil. Most of the time just a particle of dust can introduce Rhiz.
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nobutreally
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Interesting.
I'd have to disagree about it being Rhizopus, all the examples I can find of any varieties of Rhizopus look more "blobby" and don't seem to present with the black dots quite like this. With a little digging it almost certainly appears to be Aspergillus niger, though.
C'est la vie, so she goes. I was hopeful that the black dots were "on top" of decent mushroom culture but it doesn't seem that way.
Edited by nobutreally (01/31/24 04:34 PM)
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GenesisCorrupted
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Re: AGAR ENVY! (Anything and All things agar!) [Re: nobutreally]
#28642144 - 01/31/24 05:07 PM (3 months, 15 days ago) |
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It forms long hairs with black dots at the end. There is nothing underneath it. Except more mold.
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AdderLadder
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bigworm] 1
#28642298 - 01/31/24 07:01 PM (3 months, 15 days ago) |
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Quote:
Bigworm said:

Where did you get your spores from? No vendor names just a general thing like online or a friend, a trusted vendor, ect..
I got the spore swabs from a reputable website, ditto the agar trays, there definitely is a lot I need to do differently next time 🙈
-------------------- Mushroom growing newbie 
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Bigworm



Registered: 03/19/22
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Loc: Murica
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Re: AGAR ENVY! (Anything and All things agar!) [Re: AdderLadder]
#28642432 - 01/31/24 09:23 PM (3 months, 15 days ago) |
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That's is how we all learn.
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GenesisCorrupted
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Re: AGAR ENVY! (Anything and All things agar!) [Re: tree frog]
#28642436 - 01/31/24 09:26 PM (3 months, 15 days ago) |
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Quote:
tree frog said: Is it worth the effort to clone a slightly soggy abort if it displayed interesting genetics/possible mutation?
This Tamp abort for example?

Out of curiosity, I would. But only if it isn’t a burden to you. It would just be something to play with.
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koma23



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Re: AGAR ENVY! (Anything and All things agar!) [Re: GenesisCorrupted] 2
#28642576 - 02/01/24 02:18 AM (3 months, 14 days ago) |
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I'm attempting, for the first time, to clone some fruit on Agar. Actually, this is my first time working with agar as is.
I'm not quite sure if I want to isolate, I'd be happy just with clones. However, a question arises as the samples show really pronounced rhizo lines; if I was diligent and cut out just these single lines, would that be the way to go towards an isolate, or should I wait for them to spread out?
And as for creating a clone and not a single culture, how should I proceed? I used to work with LC only when cloning, but this is different, as when I'll take a certain slice out of the agar, this wont be a clone anymore right? (The first sample has a contam but I want to salvage it as it's the only viable sample from the best fruit).



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chris77
Archaic Revivalist



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Re: AGAR ENVY! (Anything and All things agar!) [Re: koma23] 2
#28642584 - 02/01/24 02:41 AM (3 months, 14 days ago) |
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Quote:
koma23 said: I'm attempting, for the first time, to clone some fruit on Agar. Actually, this is my first time working with agar as is.
I'm not quite sure if I want to isolate, I'd be happy just with clones. However, a question arises as the samples show really pronounced rhizo lines; if I was diligent and cut out just these single lines, would that be the way to go towards an isolate, or should I wait for them to spread out?
And as for creating a clone and not a single culture, how should I proceed? I used to work with LC only when cloning, but this is different, as when I'll take a certain slice out of the agar, this wont be a clone anymore right? (The first sample has a contam but I want to salvage it as it's the only viable sample from the best fruit).




first off, these are beautiful, well done.
and the answer is yes, cut out a part of a line and you are greatly narrowing down the gene pool. you could then transfer once more (selecting for speed or vigorous growth) and then grow out that culture. select the best fruit (for what you prefer) and take a spore print. grow tgis one out, then select from the resulting fruit and continue cloning etc repeat….
the key word and what you are showing here in your examples is ‘sectoring’. read a bit on that.
even within a tiny sector of one of the ‘lines’ you will have a huge amount of genetic variability. so.. working with that will still give a wide variety from different fruit, but it is definitely already narrowed down by a big margin.
-------------------- the observer is the observed j. krishnamurti
Edited by chris77 (02/01/24 02:42 AM)
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koma23



Registered: 09/15/20
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Re: AGAR ENVY! (Anything and All things agar!) [Re: chris77]
#28642602 - 02/01/24 03:31 AM (3 months, 14 days ago) |
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Quote:
chris77 said:
Quote:
koma23 said: I'm attempting, for the first time, to clone some fruit on Agar. Actually, this is my first time working with agar as is.
I'm not quite sure if I want to isolate, I'd be happy just with clones. However, a question arises as the samples show really pronounced rhizo lines; if I was diligent and cut out just these single lines, would that be the way to go towards an isolate, or should I wait for them to spread out?
And as for creating a clone and not a single culture, how should I proceed? I used to work with LC only when cloning, but this is different, as when I'll take a certain slice out of the agar, this wont be a clone anymore right? (The first sample has a contam but I want to salvage it as it's the only viable sample from the best fruit).




first off, these are beautiful, well done.
and the answer is yes, cut out a part of a line and you are greatly narrowing down the gene pool. you could then transfer once more (selecting for speed or vigorous growth) and then grow out that culture. select the best fruit (for what you prefer) and take a spore print. grow tgis one out, then select from the resulting fruit and continue cloning etc repeat….
the key word and what you are showing here in your examples is ‘sectoring’. read a bit on that.
even within a tiny sector of one of the ‘lines’ you will have a huge amount of genetic variability. so.. working with that will still give a wide variety from different fruit, but it is definitely already narrowed down by a big margin.
Hi, tnx mate!
Yeah, sectoring, don't know why I didn't use that term. Also, strands would have been better then lines : ) Sure, I've looked at what sectoring is before, but I've always seen it refereed to certain elliptical/circular parts of the growth and wasn't sure about these strands ...
I'm not sure if I should wait or start transferring now; will these strands branch out in to sectors and form circular/elliptical mycelium? And is that a better time to make transfers? Or should I just isolate these tiny strands now and transfer?
Edited by koma23 (02/01/24 03:33 AM)
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chris77
Archaic Revivalist



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Re: AGAR ENVY! (Anything and All things agar!) [Re: koma23]
#28642604 - 02/01/24 03:40 AM (3 months, 14 days ago) |
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imo they won’t branch out . take some of the tips that appeal to you. follow your instincts transfer them and grow them. out  you are on a good path here!
btw, what are we looking at here? cubensis i imagine?
-------------------- the observer is the observed j. krishnamurti
Edited by chris77 (02/01/24 03:41 AM)
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koma23



Registered: 09/15/20
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Re: AGAR ENVY! (Anything and All things agar!) [Re: chris77] 1
#28642609 - 02/01/24 04:00 AM (3 months, 14 days ago) |
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Quote:
chris77 said: imo they won’t branch out . take some of the tips that appeal to you. follow your instincts transfer them and grow them. out  you are on a good path here!
btw, what are we looking at here? cubensis i imagine?
I think I'll let them grow for a bit as I'm curious what'll happen. Trying and learn ...
Yeah, these are B+. For starters said I'll pick a forgiving strain.
Tnx again : )
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