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mycosispeon
Goofy's bastard child


Registered: 05/17/23
Posts: 185
Loc: PNW
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OP updated
It was time to make T2s of the Ghost. The TAT is lagging a bit and I ended up deciding to make some new T1s.
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ReverendMyc
succinct is not my forte

Registered: 03/29/19
Posts: 1,849
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2024-01-23 I actually executed the plan. Don't tell my wife that I can do that.
  subtropicalis bell cap went to t2s and 2x1 pint millet grain jars.
-------------------- Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023 Don't Panic   
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fiddle_head
I´m not the dude, guy



Registered: 08/05/08
Posts: 2,448
Loc: luminiferous ether
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Re: LAGM v. 2.024 [Re: Noncense] 2
#28635130 - 01/26/24 12:25 AM (3 months, 20 days ago) |
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Edited by fiddle_head (02/23/24 02:17 PM)
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edixo
Fun Guy


Registered: 12/21/23
Posts: 100
Loc: Noosphere
Last seen: 15 days, 6 hours
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Re: LAGM v. 2.024 [Re: edixo]
#28635156 - 01/26/24 01:28 AM (3 months, 20 days ago) |
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Quote:
edixo said: 26/01
Grain transfers are slowly coming in. However, something else has happened - I accidentally dropped one of the transfer plates while looking at it, which caused the transfer wedge to split into three. Two stayed in the middle, and another went to the edge of the plate. Some bacterial contamination sprung up right next to it, and lo and behold; the myc beat it. It's slowly surrounding the bacteria, and the myc growing from it is markedly thicker and more even than the other wedge pieces.
I think I'm gonna isolate it.

-------------------- ~ LAGM 2024 ~
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edixo
Fun Guy


Registered: 12/21/23
Posts: 100
Loc: Noosphere
Last seen: 15 days, 6 hours
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Re: LAGM v. 2.024 [Re: edixo]
#28636391 - 01/27/24 01:48 AM (3 months, 19 days ago) |
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Quote:
edixo said: 27/01
Not even 24 hours after transferring that contam resistant strain, and it looks like this. Exciting!

-------------------- ~ LAGM 2024 ~
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mycosispeon
Goofy's bastard child


Registered: 05/17/23
Posts: 185
Loc: PNW
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Re: LAGM v. 2.024 [Re: edixo]
#28636839 - 01/27/24 11:53 AM (3 months, 19 days ago) |
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Updated OP
The original T1 of TAT has one "cord" that is breaking away from the rest. Is that just a rogue strain? Or two that joined together?
These pix are 4 days apart
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mnj
Rad Visuospatial Sketchpad



Registered: 11/23/08
Posts: 679
Loc: Inner Space
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This is such a great question. I'm leaning toward the possibility that they joined together from my visual interpretation, but I don't have much knowledge or experience of how mycelium acts, so take my opinion for what it's worth, which is probably not much.
-------------------- LAGM 2024
Thank yoU please come again FrienD
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rhizoRider
Mycorrhizally expanding



Registered: 12/24/13
Posts: 2,483
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Re: LAGM v. 2.024 [Re: mnj]
#28636968 - 01/27/24 02:01 PM (3 months, 18 days ago) |
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--------------------
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 7,071
Loc: Rent free in your head
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I believe I got germination on one of the wild Alabama cube plates

The other plate has a spot of mold on the edge.

Nothing on the Florida plates
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖
    "Mist your balls and fan your asshole" - Pandaskis, 2023
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YellowBelly
Stranger
Registered: 08/29/23
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-------------------- LAGM 2024
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mycosispeon
Goofy's bastard child


Registered: 05/17/23
Posts: 185
Loc: PNW
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OP updated.
A few questions though. Do you agree with my sector sections (good in red, bad in blue) and is it too soon to do another round of isolation? The TAT plates are T1, and the Ghost plates are T2.
TAT
 Ghost
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Gastronomicus
3-0-G



Registered: 03/31/05
Posts: 9,746
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Damn those are gorgeous plates. Yeah red sections look good and you can transfer now
-------------------- Make my Funk the P Funk, I wants to get Funked up
LAGM2024
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ReverendMyc
succinct is not my forte

Registered: 03/29/19
Posts: 1,849
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OP updated 2024-01-30 First shake of bell cap subtropicalis jars. Looking good so far. Forgot to snap a picture.
So far nothing from the GT plates or pucks. And still nothing from from the subtropicalis xica or 0t0erance.
 
But it looks like time to put subtropicalis Hindsight, Mexicana Chicon Nindo, and pan bunnell to grain soon.
-------------------- Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023 Don't Panic   
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Pnin
Riz Gukgak


Registered: 07/18/23
Posts: 486
Last seen: 1 day, 16 hours
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Re: LAGM v. 2.024 [Re: Pnin] 2
#28641142 - 01/30/24 09:06 PM (3 months, 15 days ago) |
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OP updated!
Quote:
Pnin said: 1/30 Country cock and phobos get another transfer. Lucid gates plate sent to a pint of Crack's no prep millet.



-------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿 >my end of 2023 grow journal<
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SWBZA



Registered: 05/09/23
Posts: 207
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SWBZA: LAGM v. 2.024 - Golden Teacher [Re: Noncense] 1
#28641204 - 01/30/24 10:40 PM (3 months, 15 days ago) |
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Golden Teacher spore print to fruit
This will be a nostalgic journey for me. I'm using the very first spore print I made in July 2023, and I made it from the very first shroom I ever got to fruit.
31 January
This is my spore print after taking two swabs.

I've taken the spores to two plates - one a normal LME, and with the other one I'm playing. I've read somewhere how you can make serial dilutions. I put my second spore scrape in 20ml of sterile water, shook the living daylights out of it hoping to mix it well, and then put two drops of that on a plate. I then put 1ml of that liquid on another 20ml sterile water, and put 2 drops of that on plate 2. Same thing, 1:20 dilution going to plate 3. Let's see what happens. I expect "nothing", but who knows. It could be fun seeing colonies growing together, or something like that. I'll post pictures of the plates once something starts happening (or not!). For now it will just be a photo of an empty petri dish 
2 February
The spore streak start:

It looks like the majority of the spores were deposited right at the start of the streak. Note to self: the streak started way to close to the edge of the plate. Next time, start streak much nearer to the centre of the agar.
There is something growing further along the streak:
Very fast, so probably bad news Hard to photograph - still quite small.
There is a little spot growing on dilution plate 1. I can see it with the naked eye, and with light behind it and a magnifying glass, I can see little filaments growing - similar to the photo above for the spore plate. Not for lack of trying, I simply can't take a photo yet.
4 February
Solid growth (but of what? ), and it looks like a spot of contam. Thinking of taking a transfer at 4 o'clock. Making agar tonight - slept on the job, so no new plates available. Would have loved to have the new plates sit for a week to eliminate bad ones. Oh, well, bad planning.

5 February
T1 done. Nothing on serial dilution plates. Oh, well, had to play. Now we wait.
6 February
Did two T1s. This one was done with a needle biopsy tool I made - smaller than a grain of rice. First LME + yeast plates (they look butt-ugly, if you ask me - murky plates, not sure I like adding yeast). Growth looks way too much for one day. Mold? I've posted a question in the Agar thread and asked for a plate read under "contam".

7 February
Views from the top and through for the two T1 plates. Took T2 from the "small biopsy" plate as I don't trust that dark stuff growing from the middle. First thought was that the myc is taking on the blue from the original plate, but no longer sure.
Original as per this morning:

Scalpel T1:


Tiny biopsy T1:


Not happy today. Want to do end-to-end agar, not go to BRF puck to get to fruit. Not the point of this exercise.
8 February

End of the road for this journey Maybe I'll get brave some other day and try this print and / or some others again. Enough for now.

==================================================================
We don't give up, so we try again - else no learning
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10 February

Spore streaks to agar using sterile loop.
- Pink Oyster (who can not get pink oyster growing?
)
- Brown Oyster
- Golden Teacher - recent print that I made myself
- Golden Teacher - the problematic print used at the start of this post. Let's see whether it is the print or my technique.
14 February
The four plates were bacterial - the whole batch I made was rubbish. All went to the bin, including these spore plates.
Used one of the new plates I made after the disastrous LME / Yeast plates debacle, and tried yet again. Something's growing where I'd expect something to be growing:

Wait and see. Again.
Took 3 Josex poke size transfers to water agar, one normal to LME agar. Too soon, I don't care. Felt like it.
15 February
Playing around. Took a Jack Frost, scraped the gills of a new fruit, streaked a plate. Just for fun.
22 February
The IS plate today:

The three pokes on water agar - looks like one nasty riding along:

Two T1s:


24 February
Tweezer biopsy

My Jack Frost gill scrape. Time to clean it up.

25 February
Took one small biopsy from cleanest spot I could find, to LME. Took two tweezer samples to same plate. Both showed good growth, but looking at the bottom of the plate, they looked like they were growing on top of bacteria. No other clean places on gill scrape plate that looked properly clean. Want to see whether the bacteria travelled with when I lifted a strand (as best as I could) from the top. If the transfers are dirty, I will try taking a transfer from that plate to tea agar to see whether the bacteria then slow down or not grow at all.
28 February
My little Inca Stargazer project today

The most promising plate:

Tweezer biopsy from it:

Will consider taking a sample of this one to grain if it keeps growing well.
Also messing around on water agar. I keep wondering about the "stuff" running ahead of what looks like myc. Took a biopsy of "this looks like myc" close to the transferred agar, but also the smallest biopsy I could from the very leading edge of the "what is this running ahead?" part of the plate.
This is the transfer of "I think this could be myc":

This is from the very leading edge - the "what is this?":

It is beautiful, whatever it is...

29 February
Took the tweezer biopsy to grain. Very small wedges. Probably to early. Don't care.
Quite disappointed with the popcorn jars. I dried them with paper towels after I was convinced they are tissue dry. There were still small white kernels when I drained them and left them to dry. There was foil over the lids. So much for steam won't wet the grains. Despite hot shake yesterday, today those grains are wet. One has modified lid and I made sure the lid is tight. The other two are unmodified, so who knows what can get in there and enjoy the wet grains. So very disappointed with the popcorn tek.
Edited by SWBZA (02/29/24 06:33 AM)
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mnj
Rad Visuospatial Sketchpad



Registered: 11/23/08
Posts: 679
Loc: Inner Space
Last seen: 3 hours, 1 minute
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Re: LAGM v. 2.024 [Re: SWBZA]
#28641352 - 01/31/24 04:37 AM (3 months, 15 days ago) |
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Not too late at all.
I think the only rule is you must start by putting spores to agar, then you can grow however you like.
Welcome!

-------------------- LAGM 2024
Thank yoU please come again FrienD
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SWBZA



Registered: 05/09/23
Posts: 207
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Re: LAGM v. 2.024 [Re: mnj]
#28641362 - 01/31/24 04:55 AM (3 months, 15 days ago) |
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Quote:
mnj said: Not too late at all.
I think the only rule is you must start by putting spores to agar, then you can grow however you like.
Welcome!
 
Great stuff, thanks. In anticipating a sweet adventure, I took the very first spore print I ever made (Golden Teacher, July last year) to agar, and just because why not, I also did my very first serial dilution - spore scrape to sterile water, two dilutions 1:20. Made 3 agar plates for those. I actually have no idea what I'm doing, think I saw a YouTube video months ago about this But why not! This is a fun hobby for me and I'm hoping to learn by doing.
Now to get going with my first ever spore to fruit journey. This is going to be fun.
Cheers!
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,736
Loc: Gamehenge
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Quote:
mycosispeon said: OP updated.
A few questions though. Do you agree with my sector sections (good in red, bad in blue) and is it too soon to do another round of isolation? The TAT plates are T1, and the Ghost plates are T2.
TAT
 Ghost

Those are looking good! I don’t think you can go wrong with the TAT plates, even the blue transfer piece. With that said though I like the 2nd TAT plate more than the first. All the red looks great on Ghost. I see good things in your future
--------------------
I 5318008 NOT a virgin!
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mycosispeon
Goofy's bastard child


Registered: 05/17/23
Posts: 185
Loc: PNW
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Quote:
bigfootscreepyuncl said:
Quote:
mycosispeon said: OP updated.
A few questions though. Do you agree with my sector sections (good in red, bad in blue) and is it too soon to do another round of isolation? The TAT plates are T1, and the Ghost plates are T2.
TAT
 Ghost

Those are looking good! I don’t think you can go wrong with the TAT plates, even the blue transfer piece. With that said though I like the 2nd TAT plate more than the first. All the red looks great on Ghost. I see good things in your future 

I'm going to perform those isolations tonight
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ReverendMyc
succinct is not my forte

Registered: 03/29/19
Posts: 1,849
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Tiny update
2024-02-01
 Subtropicalis Hindsight. Sending these t1 transfers to grain after getting some t2s from them. Going to wait on the pans and mex that are moving slower.
-------------------- Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023 Don't Panic   
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