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Invisiblemycosispeon
Goofy's bastard child
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Registered: 05/17/23
Posts: 185
Loc: PNW
Advice on spawn cleanliness/readiness * 1
    #28640543 - 01/30/24 12:22 PM (3 months, 16 days ago)

Looking for some feedback on the cleanliness and readiness of these spawn jars.

All eight are rye berries, prepped using SpitballJedi's How I do Rye Berry Prep write up. I inoculated them with a single 1"x1" agar wedge back on 1/8, they colonized slowly (I wondered if I had to much FAE due to lids having x2 3/8" holes covered with SFP each.) On 1/22 they were at maybe 20% colonized, so I did a break and shake. They have taken off since. Four are JMF, four are Mazatapec.

All four of the JMF jars are around 90% colonized today.


Two of the Maz jars are almost fully colonized. One is 90%, and the other is 75%.


Do they look clean? How close to being able to spawn them to bulk?

Edited by mycosispeon (01/30/24 12:26 PM)

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OfflineLewDoja
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Re: Advice on spawn cleanliness/readiness [Re: mycosispeon]
    #28641243 - 01/30/24 11:48 PM (3 months, 15 days ago)

I personally like to shake at 95-100% to see how fast she recuperates. If it's slow or only the top does then more like to be some contamination.

Do you have other Jars ?  You may want to try g2g with some of these-  if it's clean of course... g2g will colonize more rapidly.


Hard to tell from pics but I'd say you're good - wait for a day or two after 100% to make sure unless you shake again and wait for complete mycelium takeover.


If you notice any grains that have wetness pressed against  glass keep eye out for any tinge/milky coloration to the wetness (bacterial) or any funky myc (aerial, spikey, or supper rhizo looking patches ) means it's battling or running from contam.


--------------------
a wise man said:
"Bad drugs tell you, that you want more;
  Good drugs tell you, that you've had enough"



      Trades pending:
if we have any pending trades or you never received anything that you were expecting send a PM with details.  I've had a lot going on, and may have overlooked something as well as USPS snafu's.

Edited by LewDoja (01/30/24 11:52 PM)

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Invisiblemycosispeon
Goofy's bastard child
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Registered: 05/17/23
Posts: 185
Loc: PNW
Re: Advice on spawn cleanliness/readiness [Re: LewDoja]
    #28641259 - 01/31/24 12:34 AM (3 months, 15 days ago)

I do not have any other jars prepped, no. I've read through Stipe-n Cap's Clean spawn checklist and from what I can tell all four look clean. There's no grains smashed wet to the glass, almost zero moisture, and only 2 of the jars have any metabolites and it's like two spots that are so small, I'm not even sure if it is metabolite or just an exploded grain. I think I'm going to reassess tomorrow and if it's 100% colonized, I'll wait another 24 hours and then I think it'll be "go time".

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OfflineHelloImBob
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Registered: 03/30/08
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Re: Advice on spawn cleanliness/readiness [Re: LewDoja]
    #28641452 - 01/31/24 07:04 AM (3 months, 15 days ago)

Quote:

LewDoja said:
I personally like to shake at 95-100% to see how fast she recuperates. If it's slow or only the top does then more like to be some contamination.

Do you have other Jars ?  You may want to try g2g with some of these-  if it's clean of course... g2g will colonize more rapidly.


Hard to tell from pics but I'd say you're good - wait for a day or two after 100% to make sure unless you shake again and wait for complete mycelium takeover.


If you notice any grains that have wetness pressed against  glass keep eye out for any tinge/milky coloration to the wetness (bacterial) or any funky myc (aerial, spikey, or supper rhizo looking patches ) means it's battling or running from contam.





I agree it never hurts to wait until they are fully colonized to give them an extra shake and wait a day or two to ensure they recover quickly.

Looking good though!


--------------------
Quote from Stipe-n-Cap

"You appear to be talking about boosting tryptamine content in mycelium by amending LC with....whatever your amendments are. I have to say I'm a tad disappointed that you're addressing us with the shorthand of a 13 yo girl who's texting her besty for make-up tips, instead of proper English, which causes me to have doubts."

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Invisiblemycosispeon
Goofy's bastard child
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Registered: 05/17/23
Posts: 185
Loc: PNW
Re: Advice on spawn cleanliness/readiness [Re: HelloImBob]
    #28643906 - 02/02/24 12:08 PM (3 months, 13 days ago)

Good news


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Invisiblemycosispeon
Goofy's bastard child
 Unread Journal

Registered: 05/17/23
Posts: 185
Loc: PNW
Re: Advice on spawn cleanliness/readiness [Re: mycosispeon]
    #28647549 - 02/05/24 11:52 AM (3 months, 10 days ago)

It's taking longer than I expected to quite get back to where it was, thickness-wise at least. It appears 100% colonized, area-wise though.


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Invisiblejoze
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Registered: 11/10/20
Posts: 1,056
Loc: PNW
Re: Advice on spawn cleanliness/readiness [Re: mycosispeon]
    #28647648 - 02/05/24 01:31 PM (3 months, 9 days ago)

Looks good! Your slow growth could be from low temperatures, but it's also possible that your grains are a tiny bit under-hydrated. Obviously too much moisture isn't good, but if they're dry at all it will slow down the growth significantly.


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OfflineAlexandrDughin
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Registered: 10/30/23
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Re: Advice on spawn cleanliness/readiness [Re: mycosispeon]
    #28647704 - 02/05/24 02:36 PM (3 months, 9 days ago)

i just shook a jar that was 99% colonized ( very close to full...), at the same time with 2 other jars that were 60-70% colonized.

What i noticed on the ar that was almost full is that it is not growing aggresively new micelium, just filling a few spots on the same grain that have some small area free.

I suppose it behaves like that because mycellium will not grow " on top ", over, a grain that is already colonized ?

left jar : shaken at 99%, right jar at 60% plus minus...


feedback on this behaviour from more experienced growers is welcome :smile:

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