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OfflinePhreshmaker
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Registered: 12/26/23
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Last seen: 24 days, 7 hours
I tried to get back with 7 year old spores. i think i messed up
    #28640469 - 01/30/24 11:18 AM (11 months, 11 days ago)

Hi there.
its about 7 years ago that ive made a spore print of b+ and stopped cultivation.
Now i wanted to start again and tried to get the spores germinated.
Ive made some normal spore syringes and put them onto grain and on agar.
After weeks of waiting there was still nothing to see. No contamination and no germination.
Than ive put some spores with lc solution in a syringe and put a rubberband on it for some preasure. A week later ive saw some mycelium on different spots inside the syringe and was so happy.

But please look at the inoculated grain. I think it looks like i messed up. What do you think?

Edited by Phreshmaker (04/15/24 01:52 AM)

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OfflineLand TroutM
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Re: I tried to get back with 7 year old spores. i think i messed up [Re: Phreshmaker] * 3
    #28640473 - 01/30/24 11:24 AM (11 months, 11 days ago)

Both look like mold. How fast is it growing?

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OfflinePhreshmaker
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Re: I tried to get back with 7 year old spores. i think i messed up [Re: Land Trout]
    #28640484 - 01/30/24 11:33 AM (11 months, 11 days ago)

its seven days old

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Invisibleelasticaltiger
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Re: I tried to get back with 7 year old spores. i think i messed up [Re: Phreshmaker] * 1
    #28640501 - 01/30/24 11:43 AM (11 months, 11 days ago)

I mean, could just be weak ass mycelium and bad lighting. Also if your agar recipe is weak in nutrients it can turn out really light.

The reason i'm leaning this way is because I made agar from the leftover water of boiled black rice once and it looked like that.





OP whats your recipe? You should transfer that top growth asap to confirm mycelium.

Edited by elasticaltiger (01/30/24 11:45 AM)

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OfflinePhreshmaker
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Re: I tried to get back with 7 year old spores. i think i messed up [Re: elasticaltiger]
    #28640509 - 01/30/24 11:51 AM (11 months, 11 days ago)

I will let it grow for a while and see whats happen. I dont have more spores or tries, so thats my only chance
I have a second jar that looked the same and i shaked it today. I will post some pics if there is more to see

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Invisibleelasticaltiger
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Re: I tried to get back with 7 year old spores. i think i messed up [Re: Phreshmaker]
    #28640516 - 01/30/24 11:56 AM (11 months, 11 days ago)

Quote:

Phreshmaker said:
I will let it grow for a while and see whats happen. I dont have more spores or tries, so thats my only chance




With that attitude it will be. Stay open. The universe provides.


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OfflineYahra
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Re: I tried to get back with 7 year old spores. i think i messed up [Re: Phreshmaker]
    #28640569 - 01/30/24 12:52 PM (11 months, 11 days ago)

Quote:

Phreshmaker said:
I dont have more spores or tries, so thats my only chance



After 90 days and 50 posts you'll gain access to the marketplace.

I am sure someone can get you started with spores again.

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OfflinePhreshmaker
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Re: I tried to get back with 7 year old spores. i think i messed up [Re: Yahra]
    #28640575 - 01/30/24 12:56 PM (11 months, 11 days ago)

That would be nice. but i hoped so much that i can get it myself started :sad:

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OfflineYahra
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Re: I tried to get back with 7 year old spores. i think i messed up [Re: Phreshmaker]
    #28640578 - 01/30/24 01:00 PM (11 months, 11 days ago)

I know.

Next time don't shoot spores directly into grain. This will fail almost every time.

Spores to agar to grain is the way to go.

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OfflinePhreshmaker
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Re: I tried to get back with 7 year old spores. i think i messed up [Re: Yahra]
    #28640602 - 01/30/24 01:17 PM (11 months, 11 days ago)

Yes thats what i did.
spores to agar and nothing happened
spores to grain and nothing too
spores to lc with some preasure to agar and grain results in the pictures

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OfflineYahra
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Re: I tried to get back with 7 year old spores. i think i messed up [Re: Phreshmaker]
    #28640606 - 01/30/24 01:24 PM (11 months, 11 days ago)

Maybe you should have rehydrated the spores for a longer time.

How long did they sit in the syringe before you used them?

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Offlinephenyl
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Re: I tried to get back with 7 year old spores. i think i messed up [Re: Yahra] * 1
    #28640691 - 01/30/24 02:29 PM (11 months, 11 days ago)

Honestly? It's a generic cube and a new syringe is like $10. Your time and materials working with that print probably costs more.


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InvisibleLadysKnight
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Re: I tried to get back with 7 year old spores. i think i messed up [Re: phenyl]
    #28640730 - 01/30/24 03:02 PM (11 months, 11 days ago)

Quote:

phenyl said:
The fool who persists in his folly will become wise.




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OfflinePhreshmaker
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Re: I tried to get back with 7 year old spores. i think i messed up [Re: phenyl] * 2
    #28640768 - 01/30/24 03:41 PM (11 months, 11 days ago)

Quote:

phenyl said:
Honestly? It's a generic cube and a new syringe is like $10. Your time and materials working with that print probably costs more.




I dont care about the time and material. to order new ones are risky for me, so why shouldnt i try first to get it startet with the things i have?

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OfflinePhreshmaker
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Re: I tried to get back with 7 year old spores. i think i messed up [Re: Yahra]
    #28640769 - 01/30/24 03:41 PM (11 months, 11 days ago)

Quote:

Yahra said:
Maybe you should have rehydrated the spores for a longer time.

How long did they sit in the syringe before you used them?




dont know exactly, maybe a week

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OfflineYahra
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Re: I tried to get back with 7 year old spores. i think i messed up [Re: Phreshmaker] * 1
    #28640775 - 01/30/24 03:50 PM (11 months, 11 days ago)

Here's a post from someone who tried to germinate 7 years old spores as well.

Took 3 to 4 weeks to see signs of growth:

https://www.shroomery.org/forums/showflat.php/Number/28638354#28638354

How long did you wait, exactly? You said weeks.

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OfflinePhreshmaker
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Re: I tried to get back with 7 year old spores. i think i messed up [Re: Yahra] * 1
    #28641224 - 01/30/24 11:10 PM (11 months, 10 days ago)

dont know. i didnt wrote it down. but it wasnt that long

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OfflineLogicaL ChaosM
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Re: I tried to get back with 7 year old spores. i think i messed up [Re: Phreshmaker]
    #28641307 - 01/31/24 02:21 AM (11 months, 10 days ago)

I think i see little black dots on the mycelium on the agar plate, which would indicate pin mold, so thats my guess.

But the mycelium in the grain jar does kinda look like weak cubenis mycelium :strokebeard:


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OfflinePhreshmaker
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Re: I tried to get back with 7 year old spores. i think i messed up [Re: LogicaL Chaos] * 1
    #28641356 - 01/31/24 04:42 AM (11 months, 10 days ago)

I think i can see what you mean with black spots. But i believe that they are are not from mycelium. Maybe its just a bad picture, some shaddows or dirt on the table?

I will let it grow and hope that i can show you something good at the end

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OfflineHelloImBob
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Re: I tried to get back with 7 year old spores. i think i messed up [Re: Phreshmaker]
    #28641364 - 01/31/24 04:56 AM (11 months, 10 days ago)

Quote:

Phreshmaker said:
I think i can see what you mean with black spots. But i believe that they are are not from mycelium. Maybe its just a bad picture, some shaddows or dirt on the table?

I will let it grow and hope that i can show you something good at the end





Yeah that's what he is staying, black pin mold has a black dot on the top of what looks like a pin/needle.

I'm sorry to say both of those look like mold to me.

I appreciate the desire to make stuff happen any way possible but your better off to become a part of the community and the members are more than happy to help and are overly generous around here.

The marketplace is a mushroom wonderland :smile:


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