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InvisibleLadysKnight
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Registered: 10/09/15
Posts: 1,777
Re: Recognizing and dealing with contamination [Re: Xerbia]
    #28638248 - 01/28/24 02:35 PM (1 month, 1 day ago)

There's a lot of mycelial growth variation out there. Might be cool.
:mitebecool:

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OfflineNichrome
I'm a torso!
I'm a teapot User Gallery

Registered: 12/17/18
Posts: 6,728
Loc: Zone 5
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Re: Recognizing and dealing with contamination [Re: Xerbia] * 1
    #28638837 - 01/28/24 10:48 PM (1 month, 1 day ago)

Quote:

Xerbia said:
Quote:

LadysKnight said:
It's got that moldy look to it. When unsure, take transfers to new plates, but hold the old plates to see what pins




Thanks. I took some small scraps to see if I could get this cleaned up any further. This was a clone of Lactarius Rubidus, but I'm starting to wonder if it's just some kind of mold. It looks and acts like mycelium, but no idea.





That is very likely your target mycelium.

It's not mold.


--------------------
Once you've got something figured out completely, you're stupid.

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OfflinePShroomaker
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Registered: 01/28/24
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Last seen: 12 days, 22 hours
Re: Recognizing and dealing with contamination [Re: LadysKnight]
    #28639122 - 01/29/24 09:28 AM (1 month, 19 hours ago)



So I have some black floating contam in some liquid culture, can I scoop it out or is it toast? It appeared that there are spores germinating at the bottom of the jar.

Thanks

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OfflineYahra
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Registered: 03/06/23
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Re: Recognizing and dealing with contamination [Re: PShroomaker]
    #28639187 - 01/29/24 10:22 AM (1 month, 18 hours ago)

The black stuff is mold sporulating.

There is more mold invisible to you.

I'd toss the entire jar. It's toast.

Sorry for your loss.

:cryaboutit:

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OfflineSmellyhobbit
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Re: Recognizing and dealing with contamination [Re: PShroomaker] * 2
    #28639267 - 01/29/24 11:46 AM (1 month, 17 hours ago)

Quote:

PShroomaker said:


So I have some black floating contam in some liquid culture, can I scoop it out or is it toast? It appeared that there are spores germinating at the bottom of the jar.

Thanks



Your question has already been answered, but something you need to understand as early as possible is contamination isn’t going to be a removable problem. Something is contaminated or it isn’t. Something is sterile or it isn’t. If you see a spec of black or green, your jar is done. Don’t waste time trying to save a ship that isn’t just sinking, it’s already sunk. Get a new jar going ASAP and toss whatever isn’t working. The sooner you do that the faster you’ll learn.

Not trying to rag on you, but it’s something new growers, myself included, don’t always understand right away. Forget the idea of something being “a little moldy.” Once you see mold it’s game over for the project in question.


--------------------
A Love Letter to New Growers
A Guide for New Growers
Growth 2023 - A Year In Review

Grow more shrooms. Eat more ass. :mushroom:


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OfflinePShroomaker
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Registered: 01/28/24
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Re: Recognizing and dealing with contamination [Re: Smellyhobbit]
    #28639359 - 01/29/24 12:54 PM (1 month, 16 hours ago)

Great, thank you all. I kind of figured it was toast but after seeing people carefully remove good mycelium from agar, I thought it might just be possible. I’m still in the beginners, contamination and developing my clean technique. Great to have folks out there like yourself for guidance, priceless! P

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OfflineSmellyhobbit
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Re: Recognizing and dealing with contamination [Re: PShroomaker] * 1
    #28639383 - 01/29/24 01:05 PM (1 month, 16 hours ago)

Quote:

PShroomaker said:
Great, thank you all. I kind of figured it was toast but after seeing people carefully remove good mycelium from agar, I thought it might just be possible. I’m still in the beginners, contamination and developing my clean technique. Great to have folks out there like yourself for guidance, priceless! P



Agar is for the sole purpose of separating good mycelium from molds and bacteria. That’s the tool you use to get clean mycelium to begin with. So that observation is a good one 👍

Once you get to grains or liquid cultures that process needs to have already been done. Those, once contaminated, are lost.


--------------------
A Love Letter to New Growers
A Guide for New Growers
Growth 2023 - A Year In Review

Grow more shrooms. Eat more ass. :mushroom:


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Offlineboomerbob
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Registered: 06/25/21
Posts: 25
Loc: Los Angeles, CA Flag
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Re: Recognizing and dealing with contamination [Re: Kinoko314]
    #28640052 - 01/30/24 12:42 AM (1 month, 4 hours ago)

Quote:

Kinoko314 said: Is your name boomerbob because you like boomers, or because you are a boomer?  Just curious.




Sorry for the delayed response, not logged in here often.  I'm close to a Boomer, but I'm earlier end of Gen X era....and I love boomers.


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OfflinePapayaKing
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I'm a teapot

Registered: 12/18/23
Posts: 40
Last seen: 2 hours, 47 minutes
Re: Recognizing and dealing with contamination [Re: boomerbob]
    #28641765 - 01/31/24 11:08 AM (29 days, 18 hours ago)

Hi guys,

Could somebody experienced help my friend to spot if this is bruising or mold?

The bag is almost fully colonized, and I cant see any of this elsewhere.




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OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,244
Last seen: 15 hours, 23 minutes
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Re: Recognizing and dealing with contamination [Re: PapayaKing]
    #28641781 - 01/31/24 11:26 AM (29 days, 17 hours ago)

Quote:

PapayaKing said:
Hi guys,

Could somebody experienced help my friend to spot if this is bruising or mold?

The bag is almost fully colonized, and I cant see any of this elsewhere.








What grain is that?


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can


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OfflinePapayaKing
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Registered: 12/18/23
Posts: 40
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Re: Recognizing and dealing with contamination [Re: Camera93]
    #28641793 - 01/31/24 11:35 AM (29 days, 17 hours ago)

Hi man,

It's rye, vermiculite, perlite and gypsum in this bag.

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OfflineJurelEnLata
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Registered: 01/23/24
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Re: Recognizing and dealing with contamination [Re: PapayaKing]
    #28642030 - 01/31/24 03:17 PM (29 days, 14 hours ago)

Is this cobweb? It looks grayish but has no particular smell. Photos taken from the top of a couple coco coir casing layers.



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OfflineAZmedic95
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Registered: 01/29/24
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Last seen: 28 days, 22 hours
Re: Recognizing and dealing with contamination *DELETED* [Re: JurelEnLata]
    #28642483 - 01/31/24 11:12 PM (29 days, 6 hours ago)

Post deleted by AZmedic95

Reason for deletion: Because

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OfflineAZmedic95
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Registered: 01/29/24
Posts: 5
Last seen: 28 days, 22 hours
Re: Recognizing and dealing with contamination [Re: AZmedic95]
    #28642489 - 01/31/24 11:23 PM (29 days, 5 hours ago)

Alright, what do y’all think?









Here’s the background:
-Midwest Grow Kits 66Q Monotub.
-Strain is Purple Mystic.
-Grain bag inoculated 12/16/2023 via spore syringe.
-Mixed the colonized grain bag with the substrate on 12/31/2023.
-Started allowing fresh air exchange via filter disks, introduced light and reduced temp 1/12/2024.
-First pins noted around 1/24/2024.
-Small dark patch with a little white noted 1/28/2024.(see picture 1)
-Dark patch and white grew very quickly over 18 hours(see pictures 2 and 3). Determined that it was most likely trich contamination and quarantined the tub 1/29/2024. I used a large cup to extract the visualized contamination, plus some. Then sprayed/saturated the inside and about 1in around the hole with 90% isopropyl alcohol(see picture 4)

It has now been 60 hours from time of extraction and I just harvested some of my flush(see picture 5). When harvesting, I looked pretty hard and I do not see any more trich growing.(see pictures 6 and 7)

Did I do the nearly impossible? Or am I just kidding myself? Let me know what you think!

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Offlinerumfor69
Bodhicitta Cultivator
Male


Registered: 08/05/11
Posts: 6,852
Loc: In the Gills
Last seen: 21 hours, 44 minutes
Re: Recognizing and dealing with contamination [Re: AZmedic95]
    #28642493 - 01/31/24 11:37 PM (29 days, 5 hours ago)

Ur surface conditions look good. That bright white shit is mold. Seems how you got to harvest and probably didn't see mold in ur spawn it was probably a bacterial infection in the spawn that created a weakness in the mycelium which allowed the mold to get a foothold later on.


--------------------

Links For Beginners And Beyond:mspoil:

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Offlinerokafogtacsuka
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Registered: 11/01/23
Posts: 19
Last seen: 28 days, 17 hours
Re: Recognizing and dealing with contamination [Re: rumfor69]
    #28642521 - 02/01/24 12:35 AM (29 days, 4 hours ago)

Hey there,

Could you please help me if this is trichoderma and if I should throw the tub away or not? Is there any way I can save the rest? Its before the first flush. I have a few other tubs in the room.

Thank you!


Edited by rokafogtacsuka (02/01/24 12:36 AM)

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Offlinerumfor69
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Re: Recognizing and dealing with contamination [Re: rokafogtacsuka] * 2
    #28642528 - 02/01/24 12:44 AM (29 days, 4 hours ago)

Quote:

rokafogtacsuka said:
Hey there,

Could you please help me if this is trichoderma and if I should throw the tub away or not? Is there any way I can save the rest? Its before the first flush. I have a few other tubs in the room.

Thank you!





So first you gotta just accept that when contamination happens in any step of this hobby. There is no saving, you cant end contamination, or beat it in anyway. It lives there now. If the sub produces some fruits away from it that aren't rotten then they are safe to dry correctly and consume. Keeping this contaminated tub around will not endanger your future grows, but you should be thinking about disposal soon. Don't get too attached, instead get more attached to trying again. Set a schedule where you create new tries every couple weeks or something(timing always startswith grain prep 😉). Overtime you will be able to assess your abilities, and strengthen your practice with the actions in the processes. I dunno if you're using agar but if you're not then you should definitely check out my links and get into agar. Hey I know...fruits are great. You want real power? Learn agar, master making beautiful cultures as a new hobby that make you just wanna put them to grain.


--------------------

Links For Beginners And Beyond:mspoil:

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Offlinerumfor69
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Registered: 08/05/11
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Re: Recognizing and dealing with contamination [Re: rumfor69] * 1
    #28642529 - 02/01/24 12:45 AM (29 days, 4 hours ago)

Btw yes that's mold, it's fucked


--------------------

Links For Beginners And Beyond:mspoil:

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Offlinerokafogtacsuka
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Registered: 11/01/23
Posts: 19
Last seen: 28 days, 17 hours
Re: Recognizing and dealing with contamination [Re: rumfor69]
    #28642545 - 02/01/24 01:27 AM (29 days, 3 hours ago)

Quote:

rumfor69 said:
Quote:

rokafogtacsuka said:
Hey there,

Could you please help me if this is trichoderma and if I should throw the tub away or not? Is there any way I can save the rest? Its before the first flush. I have a few other tubs in the room.

Thank you!





So first you gotta just accept that when contamination happens in any step of this hobby. There is no saving, you cant end contamination, or beat it in anyway. It lives there now. If the sub produces some fruits away from it that aren't rotten then they are safe to dry correctly and consume. Keeping this contaminated tub around will not endanger your future grows, but you should be thinking about disposal soon. Don't get too attached, instead get more attached to trying again. Set a schedule where you create new tries every couple weeks or something(timing always startswith grain prep 😉). Overtime you will be able to assess your abilities, and strengthen your practice with the actions in the processes. I dunno if you're using agar but if you're not then you should definitely check out my links and get into agar. Hey I know...fruits are great. You want real power? Learn agar, master making beautiful cultures as a new hobby that make you just wanna put them to grain.





Thank you mate! I successfully made a few tubs without any issues, so strange to have this with me now, with keeping all the neccessary steps to keep everything as steril as possible. I havent open the tub since putting the grain into the bulk and the holes are also covered with micropore. Anyway, I keep my eyes on it and see if I can have a few healthy fruits. Thank you for your checking!

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Offlinerumfor69
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Registered: 08/05/11
Posts: 6,852
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Re: Recognizing and dealing with contamination [Re: rokafogtacsuka]
    #28642557 - 02/01/24 01:41 AM (29 days, 3 hours ago)

Quote:

rokafogtacsuka said:
Quote:

rumfor69 said:
Quote:

rokafogtacsuka said:
Hey there,

Could you please help me if this is trichoderma and if I should throw the tub away or not? Is there any way I can save the rest? Its before the first flush. I have a few other tubs in the room.

Thank you!





So first you gotta just accept that when contamination happens in any step of this hobby. There is no saving, you cant end contamination, or beat it in anyway. It lives there now. If the sub produces some fruits away from it that aren't rotten then they are safe to dry correctly and consume. Keeping this contaminated tub around will not endanger your future grows, but you should be thinking about disposal soon. Don't get too attached, instead get more attached to trying again. Set a schedule where you create new tries every couple weeks or something(timing always startswith grain prep 😉). Overtime you will be able to assess your abilities, and strengthen your practice with the actions in the processes. I dunno if you're using agar but if you're not then you should definitely check out my links and get into agar. Hey I know...fruits are great. You want real power? Learn agar, master making beautiful cultures as a new hobby that make you just wanna put them to grain.





Thank you mate! I successfully made a few tubs without any issues, so strange to have this with me now, with keeping all the neccessary steps to keep everything as steril as possible. I havent open the tub since putting the grain into the bulk and the holes are also covered with micropore. Anyway, I keep my eyes on it and see if I can have a few healthy fruits. Thank you for your checking!




No part of the spawning to bulk process is clean in anyway. There's millions of mold and bacterial spores attached to micro particles of dust in ur space that get in there during spawning, mixing, through the micropore, and just around the lid gaps. Even very successful growers get tubs that fail here n there. If you can make 5 tubs and one fails and ur not an "expert" then you're doing good. That's a high success percentage. So just toss losses and keep moving forward cause ultimately you'll only get better with practice.


--------------------

Links For Beginners And Beyond:mspoil:

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