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OfflineVanboozin
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Registered: 12/17/23
Posts: 15
Last seen: 5 days, 23 hours
Check out this Crazy looking LC * 4
    #28638774 - 01/28/24 09:02 PM (3 months, 17 days ago)

I have made 7 isolates from my 13 year old Psilocybe cyanescens spore sprints, have master plates & slants backed up ( from a an outdoor grow in 2010 ).

I then made 7 corn syrup,malt peptone LC jars which were pressure sterilized.
Once cooled I dropped a singe wedge into each jar in my SAB.

In 2 of the 7 jars the wedge never sank & I know have myclelium growing on the surface and down from the wedge..I have been swirling the jars once a day but these floating wedges wont break up.. although it looks cool & all I dont know how I'm going to suck that up into a syringe ?



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OfflineVanboozin
Stranger
Registered: 12/17/23
Posts: 15
Last seen: 5 days, 23 hours
Re: Check out this Crazy looking LC [Re: Vanboozin]
    #28638784 - 01/28/24 09:10 PM (3 months, 17 days ago)

The wedges came from these plates
I also knocked up some grain jars




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OfflineLand TroutM
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Registered: 01/08/18
Posts: 3,159
Last seen: 13 days, 12 hours
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Re: Check out this Crazy looking LC [Re: Vanboozin]
    #28638830 - 01/28/24 10:32 PM (3 months, 17 days ago)

Cool. How did you store the print? Seems like whenever I try an older woodlover print they don’t germinate.

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OfflineVanboozin
Stranger
Registered: 12/17/23
Posts: 15
Last seen: 5 days, 23 hours
Re: Check out this Crazy looking LC [Re: Land Trout] * 2
    #28638873 - 01/29/24 12:20 AM (3 months, 17 days ago)

I took prints from fresh picked fruits from an outdoor grow, cap face down on tin foil with a glass over top for a day, then folded the foil and into a ziplock sandwich bag, I took many prints from 2 flushes, about 20 prints from each flush went into their own larger ziplock, then the 2 larger ziplocks into yet another ziplock, so I guess 3 ziplocks deep and have been in my fridge for 13 yrs and have moved 5 times.

Im no expert but had good luck it seems, a friend has been bugging me for yrs to do something with my prints, I finally decided to go ahead so searched on here and another forum to see what my chances of germinating these were. What I found sounded discouraging ( with little to no hope ). Most people said little chance after 5 yrs and forget it after 10, so I wasnt holing my breath.
I pulled out 1 print from each flush and made a syringe from each, inside my SAB I sucked up steril water and put a half dozen drops onto the print, Then used a loop made from a twist tie to loosen the spores and let them drip into a shot glass, then squirted the rest of the 10ml syringe in and sucked it all up ( same thing for the second print) . While the syringes sat I made agar perti dishes, 5 days after I made the syringes I shot about a ml from each syringe into its own shot glass then used the home made loop to streak about 5 plates each..
Like I said after reading about the low chance of germination I wasnt holding my breath, I did read about a guy with same species who germinated a 12-14 yr old print , he said he hydrated for a week or so, he also said it took 3 weeks to see any sings of germination.

I was also worried how clean my prints were since I honestly didnt pay much attention or wasnt sure how much care I took back then ( 1st time taking spore prints ) and I never dried the prints after spores dropped I just wrapped them up ( thinking about this I was worried they may have mold )

But every plate I streaked showed growth in 4-5 days from the 1st & only 2 prints I pulled out, I was blown away !!

In the fall of 2009 I found a healthy outdoor patch fruiting, I gathered mycelium and propagated that over the winter which led to the grow shown below.







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OfflineLand TroutM
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Registered: 01/08/18
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Re: Check out this Crazy looking LC [Re: Vanboozin]
    #28639018 - 01/29/24 06:55 AM (3 months, 17 days ago)

Awesome, thanks for doing this and sharing!

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InvisibleLithop
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Registered: 04/09/22
Posts: 978
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Re: Check out this Crazy looking LC [Re: Vanboozin] * 1
    #28639182 - 01/29/24 10:13 AM (3 months, 16 days ago)

Like a cyanescens jelly fish.
:jellyfish:
Great results & sick patches there too!


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OfflineLogicaL ChaosM
Ascension Energy & Alien UFOs
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Re: Check out this Crazy looking LC [Re: Vanboozin]
    #28639401 - 01/29/24 01:18 PM (3 months, 16 days ago)

Its beautiful :awepreciation:

Looks like a jellyfish. :cool2:


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OfflineVanboozin
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Registered: 12/17/23
Posts: 15
Last seen: 5 days, 23 hours
Re: Check out this Crazy looking LC [Re: LogicaL Chaos] * 1
    #28641948 - 01/31/24 02:04 PM (3 months, 14 days ago)

Finally sinking but still cant break it apart, not Ideal.2 out my 6 jars have it.


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