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Offlinewert0987
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What do we think of these grain jars?
    #28639030 - 01/29/24 07:24 AM (11 months, 12 days ago)

Would love some more eyeballs on these guys.

PE on agar. My grain was prepped with RR rye tek no issues. This is about 2 weeks in? It took off and within like 3 days the jars were fully covered in faint non-rhizomorphic growth but seemed too soon to me. The PE was so slow to grow on agar. Shook, let it go and what came back was puffy white but not uniform (here and there clusters). Wish I could tell you why but I shook a second time like 3 days ago (I know! But also read shaking more than once can show how weakened the myc are or not by possible contams?)

It’s slow to come back and today started to see a pool of what looks like metabolites on the bottoms and beads throughout. No clear contams I can spy though.

My real worry was even though my SAB technique is solid, it’s apparently not. The agar I cut from popped up with a spot of contam a few days after cutting so it got me nervous whatever that was weaseled its way in my jars. First PE grow on grain (did cakes previously) and I know it was slower and reacted differently than other varieties I’ve tried so it was harder to know what was going on. What do y’all think?






Edited by wert0987 (02/05/24 07:07 AM)

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OfflineScrewup
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Re: What do we think of these grain jars? [Re: wert0987] * 1
    #28639041 - 01/29/24 07:46 AM (11 months, 12 days ago)

They don’t look great IMO.


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OfflineVibetyme
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Re: What do we think of these grain jars? [Re: Screwup]
    #28639057 - 01/29/24 08:09 AM (11 months, 12 days ago)

Contams are sneaky af. I'd probably just toss those and start again if those were my jars. 

I usually go to at least T3 just to make sure what I'm working with is clean.

Sorry for your loss.  It happens to us all from time to time though.


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Offlinewert0987
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Re: What do we think of these grain jars? [Re: wert0987]
    #28639066 - 01/29/24 08:17 AM (11 months, 12 days ago)

Thanks guys. Yeah, I suspected as much. I have another plate I’m keeping an eye on then I’ll cook up some fresh rye and try again.

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Offlinenormalperson
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Re: What do we think of these grain jars? [Re: wert0987]
    #28639088 - 01/29/24 08:57 AM (11 months, 12 days ago)

Quote:

wert0987 said:
I have another plate I’m keeping an eye on  .



Show us the plate, please.

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OfflinemorningWood
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Re: What do we think of these grain jars? [Re: normalperson]
    #28639192 - 01/29/24 10:34 AM (11 months, 12 days ago)

If you had faint growth that colonized the whole jar in three days I'd say it's not cube mycelium.


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Offlinewert0987
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Re: What do we think of these grain jars? [Re: morningWood]
    #28639265 - 01/29/24 11:46 AM (11 months, 12 days ago)

I thought that too tbh. I’ll snap a pic of the agar plate when I’m home but it was a pain trying to get the PE plates to even start growing (it was spores I collected with a swab from the last PE grow I did) so I wondered if I happened to catch some similar looking white mold or something and not get the myc spores to grow.

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Offlinewert0987
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Re: What do we think of these grain jars? [Re: normalperson]
    #28640233 - 01/30/24 07:35 AM (11 months, 11 days ago)



I transferred to this plate about 4 days ago from the plate I cut my wedges from (need to mix up new agar. This was my last plate and had condensation building up before I used it.)

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Offlinewert0987
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Re: What do we think of these grain jars? [Re: wert0987]
    #28652562 - 02/09/24 06:50 AM (11 months, 1 day ago)

Update on these guys. I agreed the jars were seeming suspect but kept em going more as a science experiment to see what came from it. Lots of metabolites (bout an inch+ high when not tipped like in pic) but consistent fuzzy white throughout jars. I cracked a jar and smelled and it’s earthy. Not quite the mushroom smell I’ve come to look for but not bad either? Took a piece stuck to the lid off and hit it with some peroxide (not sure how true it is mold makes peroxide fizz is but gave it a go) and no fizzing at all. What do yall think I have here? Learning after things don’t go as planned is half the fun of mycology for me.




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OfflineVibetyme
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Re: What do we think of these grain jars? [Re: wert0987]
    #28652588 - 02/09/24 07:24 AM (11 months, 1 day ago)

It still looks weird but you might get lucky. 

Spawn em for science! Top fruiting it is probably a better way to go if you just wanted to get some genetics back that you could clone to agar.

:havesomescience:

Good luck and keep us updated on the progression!


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OfflineA.k.aM
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Re: What do we think of these grain jars? [Re: Vibetyme] * 1
    #28652617 - 02/09/24 07:55 AM (11 months, 1 day ago)

I’d guess some sort of mild bacteria or yeast type thing was in there and now the mycelium has grown over it.

You might get a flush off it, I’ve spawned some sketchy jars before that put out a solid flush then went moldy immediately.

I’ve never seen something that was faint white and fast enough to cover the jar in 3 days before, but I have had a few yeast contaminations and they looked terrible but didn’t seem to affect anything at all. Jars colonized right over it and tubs went like normal.


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Invisibleelasticaltiger
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Re: What do we think of these grain jars? [Re: A.k.a] * 2
    #28652706 - 02/09/24 09:39 AM (11 months, 1 day ago)

Most recent pics: There is mold in the second jar. You can see the blue-green spot in the center where it's stressed from it.

First jar is bacterial at least, you've got spotty and incomplete colonization along with a hell of a lot of sweat / excess moisture.

If you don't see any of the green patches in the first jar you could try to top fruit it by putting coir on top and misting when it looks dry.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

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Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

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OfflineMrJong
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Re: What do we think of these grain jars? [Re: wert0987]
    #28652755 - 02/09/24 10:52 AM (11 months, 1 day ago)

I second the tiger on both.

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Offlinewert0987
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Re: What do we think of these grain jars? [Re: elasticaltiger]
    #28652804 - 02/09/24 11:58 AM (11 months, 1 day ago)

Just peeked at that jar and it was the reflection from my window on the jar when I took the pic. Not seeing any mold thankfully.

Ok, I’ll give em a go and see if anything fruits. I’ll report back. Thanks guys!

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Invisibleelasticaltiger
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Re: What do we think of these grain jars? [Re: wert0987]
    #28652807 - 02/09/24 12:01 PM (11 months, 1 day ago)

Its not a reflection. You have mold in the second jar.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

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Invisibleelasticaltiger
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Re: What do we think of these grain jars? [Re: elasticaltiger]
    #28652808 - 02/09/24 12:01 PM (11 months, 1 day ago)

10 o clock from your thumb.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

Edited by elasticaltiger (02/09/24 12:02 PM)

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Offlinewert0987
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Re: What do we think of these grain jars? [Re: elasticaltiger]
    #28652935 - 02/09/24 02:15 PM (11 months, 1 day ago)

Yep that was reflection :smile: here’s the same spot in better direct light


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Invisibleelasticaltiger
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Re: What do we think of these grain jars? [Re: wert0987]
    #28652947 - 02/09/24 02:24 PM (11 months, 1 day ago)

Uhm. Yeah I can still see the mold.



There is still a blue hue in the bottom right and now i see distinct speckle white dots of mold on the less colonized grain.

Turn the jar slight counter clockwise and take another pic.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

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Offlinewert0987
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Re: What do we think of these grain jars? [Re: elasticaltiger]
    #28652964 - 02/09/24 02:36 PM (11 months, 1 day ago)

Tried to get a couple better pics. See what you think of these:




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Invisibleelasticaltiger
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Re: What do we think of these grain jars? [Re: wert0987]
    #28652970 - 02/09/24 02:41 PM (11 months, 1 day ago)

Im so sorry. I meant rotated slightly on its vertical axis as if we're kind looking at the grain from a different angle. Not the same angle rotated around depth axis.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

Extras: Filter Print Post Top
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