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ReverendMyc
succinct is not my forte

Registered: 03/29/19
Posts: 1,849
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Bout time to start cookin some more poop.
-------------------- Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023 Don't Panic   
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tregar
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Re: Official Copelandia/Panaeolus Thread [Re: ReverendMyc] 2
#28636425 - 01/27/24 05:32 AM (3 months, 19 days ago) |
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This is my thread on 50 pan cyan trip reports and combo with 70mg THH or pure tetrahydroharmine: https://www.shroomery.org/forums/showflat.php/Number/28108398/fpart/1/vc/1
Sterilite 54 or 32 Quart Clear Gasket Box with Blue Latches & Gasket
--> Bod's Unmodified Monotub TEK: https://www.shroomery.org/forums/showflat.php/Number/22337800/fpart/1/vc/1
Bod said "This tub fruiting method is extremely low maintenance. No fanning is required, mist if needed, which will be infrequently. The lid is NOT latched down, EVER."
--> Baba Yaga Panaeolus in monotubs: https://www.shroomery.org/forums/showflat.php/Number/27637997/vc/1
Baba Yaga said "Member kirkeng convinced me with his well performing unmodified tubs to give unmodded a try and I have to say that these are my new favorites. With those I fruit right at spawn and run subs without a casing. The lid goes on upside down." ------------------------------------------------------------ Great news!
I ditched the martha tent (only use it to grow out grains, jars, or mycobags, love both). As I had to throw out the cake pan from the attempted fruit in martha tent due to it just not fruiting, lack of pins. Too many variables to adjust and trial and error for a martha at least for this beginner.
And put the estero pan cyan substrate cakes which I birthed and 2 new estero pan cyan completely colonized substrate from the mycobags into 2 cake pans, this is what they look like only 3 days later, almost reconsolidated back together as pure white snow all over the top.
And bought 2 of the 54 quart sterilite clear gasket tubs, put the birthed cakes into it, along with the 2 cake pans.
I put programmable seedling heat mats under the tub but separated by can lids, so not touching, set thermostat to keep it at 80 degrees below, and the tubs stay at 76 degrees even when it's 55 degrees outside and around 70 degrees F inside the house at night. The air outside is humid due to lots of rain outside recently.
Punched holes in a piece of box cardboard to run the LED string light that comes on a reel, and plugged it into the timer that turns it on from 7am till 7pm.
The lid is of course put on upside down as per Bod's tek in first link below, ceiling fan off but I do keep the window cracked 1/4" behind the blinds which lets in a bit of fresh air from outside.
I misted only once the 1st day, and already see baby mushrooms all over the 7 jar cakes, the 2 cake pans should finish substrate reconsolidation after being moved from 100% colonized mycobag to the cake pans in about 3 more days, the mycelium is moving super fast to make it like snow all over the top.
Here is an estero pan cyan mushroom I picked from one of the cakes already.
I did not have any pins or mushrooms what so ever using expensive humidifiers and dual reptile heater at bottom with adjustable fan, while using the martha tent for almost over a month now...and all the sudden after moving into the Bod's tubs I have mushrooms in just 2 days!
The humidity stays between 94 to 99% all the time in the tub and the temp at 76 degrees F as I have electronic temp/humidity gauges in each tub.
I am so excited as the martha tent did not work for me, but the tubs rule at least in my case, so happy! I see baby mushrooms popping up all over the cakes just since yesterday!
Thank you Bod and Baba Yaga !!!!!!!!!!!!!
Pic 1: I'm growing in two of the 54 quart sterlite tubs, and it's working !!! Pic 2: Inside of one of the two tubs, the 7 birthed cakes the very first day they were put into tub, no mushrooms 1st day, but by next day, I have baby mushrooms all over the cakes, will take another pic when full grown. Pic 3: I have a Vivo Home seedling heat mat 10" x 20.75" under each tub with can lids as spacers, and they fit perfectly and keep it perfect temp inside even with it being 55 degrees outside. Pic 4: This estero pan cyan mushroom was picked already. Pic 5: Piece of cardboard with punched holes on each end from large box with LED strip light reel is the lighting fixture. Pic 6: Timer to keep lights on for 12 hours Pic 7: LED strip light reel for price of a movie ticket. Pic 8: Programmable seedling heat mat set to 80 degrees F underneath jar lids keeps it 76 degrees inside tubs during this January winter, even when it dips down to 50 degrees outside and 68 degrees in the house at night. Temp sensor probe for mats is stuffed underneath the heat mat itself as seen in 1st and 5th photos. Pic 9: My pan cyan substrate broken up and added from mycobag seen fully reconsolidated in the cake pans or pyrex dish after 6 days. Pic 10: Even adding a bit of grown out mycelium grain to jars full of sterilized horse poo/straw/vermiculite substrate become colonized in record time. I simply bought pre-packaged and pre-pasteurized 2.5 lbs bags of horse poo mixture substrate on line, added 2 cups or so to each pint jar, put lids on, sterilized at 15 psi for 1 hour, then in front of flow hood, poured some mycelium grain in, shook up, and it colonizes in a week. Pic 11: The wide mouth jar cultivation lids with injection port and 0.2 micron filter can be bought on line for super cheap Pic 12 and 13: The grain I started with, added wedges from Florida estero pan cyan agar plates to grain in front of flow hood.

Edited by tregar (01/27/24 07:07 AM)
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tree frog
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Re: Official Copelandia/Panaeolus Thread [Re: tregar] 2
#28636581 - 01/27/24 08:27 AM (3 months, 19 days ago) |
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I can see how THH would be a nice combo with Pans. Bisporus have been relatively gentle so far but my second trip the fear did get into me a bit.
THH would have helped ground that without causing a substantial change in potency or duration, unlike its two siblings (harmine and harmaline).
Also tregar, I tried the mat under the tubs and the substrates dried out. Half my trays died, the other half fruited okay. I had a whole layer of perlite between the mat and the tote, which isn't an air gap but still a gap anyway.
Ended up building a jcm tote for the winter but another solution is to put the mats on your lid. Then the heat comes from the top, mimicking the sun.
You're lighting might suffer though.
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Pnin
Riz Gukgak


Registered: 07/18/23
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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] 2
#28636721 - 01/27/24 10:18 AM (3 months, 19 days ago) |
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-------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿 >my end of 2023 grow journal<
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rhizoRider
Mycorrhizally expanding



Registered: 12/24/13
Posts: 2,483
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Re: Official Copelandia/Panaeolus Thread [Re: Pnin]
#28636736 - 01/27/24 10:28 AM (3 months, 19 days ago) |
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plz post bruising frui pics when ur done ✔️
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
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Re: Official Copelandia/Panaeolus Thread [Re: rhizoRider] 5
#28637135 - 01/27/24 04:42 PM (3 months, 19 days ago) |
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Treefrog encouraged me to try “some” poo in the grain spawn.. fastest I ever seen Bisporus take off 😱

Inoculated 1-15 with 10cc LC
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AspectOfTheCreator
Mastering the Art of Success



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Re: Official Copelandia/Panaeolus Thread [Re: rhizoRider] 1
#28637144 - 01/27/24 04:47 PM (3 months, 19 days ago) |
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First time trying to make some prints of actual quality in a long time. Just put some foil in a shoebox and put some caps on it. Misted the shoebox to keep humidity up then wiped it afterwards when aiming to let the prints dry. After 12 hours I shifted the caps down to try getting another print. Results were somewhat mixed.. Will these work? Will they be good enough for giveaways?
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
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I wouldn’t have wiped the tub down until after they printed. I do the same thing and then let me dry in front of the ffu. If no ffu. I thinking wiping it down would be a good idea. Or removing the top and placing in a closed SAB..
Or are you asking if double printing works? Because it definitely does
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AspectOfTheCreator
Mastering the Art of Success



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Re: Official Copelandia/Panaeolus Thread [Re: SupaThaRipper]
#28637248 - 01/27/24 06:04 PM (3 months, 19 days ago) |
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I only wiped them down after printing. Just asking if this process is correct really and how clean theyre likely to be. Id like to get some prints that are up to par with ones Ive received from people here.
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SupaThaRipper
Genetics Hoarder



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I do it the same way man and mine turn out fine. I used to use water. I’ve been using h202 lately and experimenting a lot with it. (Although is claimed to not have a use in this hobby and many days it’s worthless) so take that into consideration lol
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tregar
Stranger


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Re: Official Copelandia/Panaeolus Thread [Re: SupaThaRipper]
#28637637 - 01/28/24 06:33 AM (3 months, 18 days ago) |
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tree frog said:
Quote:
I can see how THH would be a nice combo with Pans. Bisporus have been relatively gentle so far but my second trip the fear did get into me a bit.
THH would have helped ground that without causing a substantial change in potency or duration, unlike its two siblings (harmine and harmaline).
Also tregar, I tried the mat under the tubs and the substrates dried out. Half my trays died, the other half fruited okay. I had a whole layer of perlite between the mat and the tote, which isn't an air gap but still a gap anyway.
Ended up building a jcm tote for the winter but another solution is to put the mats on your lid. Then the heat comes from the top, mimicking the sun.
You're lighting might suffer though.
Thank you tree frog and your comments over the last several months, it's been a struggle with the martha tent for over a month now, had too many variables to dial in for this beginner, just thankful this is going smoothly now with the tubs, I was about to quit for good. Live in a state where these pans grow naturally, and the temps are starting to return to normal low 70 in the afternoons, so should be able to turn off the heat mat soon. In the morning and at night it's been around 45 degrees F outside and 65 in the house. Turned heat mat that sits below jar lids down to 78 degrees F so it only keeps it at 70 degrees at night in the tub and 75 degrees in the afternoon. I have also read from another person who is on his 3rd try with pan cyan's and he was also using a martha tent with no success. The martha is a problem cause I did not know how much of the tent bottom to roll ups, how slow or fast to run the internal fan at bottom of tent, the humidifier cycling, etc, and tending to it daily with water changes, etc. I must have changed out the humidifier water every day for a month now with nothing to show for it while using the tent.
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tree frog
eats bugs



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Re: Official Copelandia/Panaeolus Thread [Re: tregar] 5
#28638897 - 01/29/24 01:37 AM (3 months, 18 days ago) |
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TTBVI pins. Cased two days ago.
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AspectOfTheCreator
Mastering the Art of Success



Registered: 12/07/22
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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
#28638917 - 01/29/24 02:20 AM (3 months, 18 days ago) |
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They grow up so fast
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tree frog
eats bugs



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Quote:
AspectOfTheCreator said: They grow up so fast
Yeah, they're so fast.
This is the poo/verm/grain jar I started about three weeks ago. For comparison, it's been so cold in my colonization area that I have a jar of nats started the same day that still isn't on bulk.
At this rate, I'll be harvesting TTBVI before I spawn those Nats.
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tree frog
eats bugs



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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] 1
#28639010 - 01/29/24 06:37 AM (3 months, 17 days ago) |
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Same spot, five hours later.
Definitely preferring the sides so far. Only a couple pins in the center.
This is a gifted culture, so not MS.
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Reishiman
Hobby grower



Registered: 08/03/23
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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
#28639035 - 01/29/24 07:37 AM (3 months, 17 days ago) |
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Quote:
tree frog said:

Same spot, five hours later.
Definitely preferring the sides so far. Only a couple pins in the center.
This is a gifted culture, so not MS.
Nice work Tree, Couple quick questions. -So I would assume that you aren't able to shake the jars to quicken colonization? -When the jars are colonized do you just use a spoon or something to get it out of the jar? -How much do you break it up before laying it in tray? Completely I would think so you can lay it flat. -Do you let it consolidate at all before casing? -Is anything added to what was in the jars besides the casing on top? -And lastly I was curious about the overall depth including casing? If you get time to answer some or all of these questions I'd appreciate it. Or if there's a tek out there you used please let me know. Trying to get all the info I can about this method before I try it out thanks. -oh and forgot to ask. Did you inoculate via l.c., agar wedge, or grain spawn?
-------------------- Seasons don't fear the reaper. Nor do the wind, the sun, or the rain.
Edited by Reishiman (01/29/24 07:39 AM)
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tree frog
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Re: Official Copelandia/Panaeolus Thread [Re: Reishiman]
#28639072 - 01/29/24 08:29 AM (3 months, 17 days ago) |
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Thanks reishiman!
Jars were inoculated with a wedge from a plate someone gifted me. Jars were about 200ml grains and 400ml poo/verm (about 50/50, there's no verm in the substrate, just coir, so the verm is here). Grains on bottom, poo/verm on top.
I shook the jars, twice actually. Once early, like 5% colonization. Because the poo and verm is on top it gives you a ton of inoculation points. So, waiting until 30% seemed like a waste. Then, I shook again at like 80% just to see how it recovered and make sure that everything was clean and colonized.
My trays are about 4 cups each. Around 2.5 inches deep. I used about 2 cups of poo/verm/grains (one small myco quart) and two cups coir. I mixed them and misted to bring to field capacity as the spawn was on the dry side.
I let the tray colonize completely and gave it a day or two to be sure it was colonized. I didn't consolidate though.
Tray was cased with 50/50 made with peat/verm, lime, and some gypsum because I had some (1 qt sifted peat, 1 qt verm, 1 tablespoon of pickling lime and gypsum). Tray was put into immediate fruiting after casing. Fruiting chamber is jcm style, but I know it's overkill, looking at other people's grows. If I wasn't impatient, and it wasn't winter, I'd be fruiting them in a shoebox most likely.
I bet with poo jars, aggressive lcs, and low to mid 70s temps for jar and sub colonization the things could be pinning in under ten days. I had low 60s to low 70s in this back bedroom and gave things an extra day here and there just to make sure things had colonized completely. And it was still really fast.
I'll break it all down in detail in a journal entry when I do jars again. But it's pretty similar to Baba's tek with his BRF/Verm mixture on top of his grains. Just I'm using poo here and then skipping the poo later.
Edited by tree frog (01/29/24 08:38 AM)
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Reishiman
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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
#28639196 - 01/29/24 10:37 AM (3 months, 17 days ago) |
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Quote:
tree frog said: Thanks reishiman!
Jars were inoculated with a wedge from a plate someone gifted me. Jars were about 200ml grains and 400ml poo/verm (about 50/50, there's no verm in the substrate, just coir, so the verm is here). Grains on bottom, poo/verm on top. (((Any reason why you layered it with the grains on bottom? Does it matter if the inoculate wedge/wedges is touching the grain spawn within the jar?)))
I shook the jars, twice actually. Once early, like 5% colonization. Because the poo and verm is on top it gives you a ton of inoculation points. So, waiting until 30% seemed like a waste. Then, I shook again at like 80% just to see how it recovered and make sure that everything was clean and colonized.
(Surprised you were able to break it up with the poo in there. I guess I'm just used to straw being in the substrates I usually use which would probably make breaking up more difficult)
My trays are about 4 cups each. Around 2.5 inches deep. I used about 2 cups of poo/verm/grains (one small myco quart) and two cups coir. I mixed them and misted to bring to field capacity as the spawn was on the dry side.
I let the tray colonize completely and gave it a day or two to be sure it was colonized. I didn't consolidate though.
Tray was cased with 50/50 made with peat/verm, lime, and some gypsum because I had some (1 qt sifted peat, 1 qt verm, 1 tablespoon of pickling lime and gypsum). Tray was put into immediate fruiting after casing. Fruiting chamber is jcm style, but I know it's overkill, looking at other people's grows. If I wasn't impatient, and it wasn't winter, I'd be fruiting them in a shoebox most likely.
I bet with poo jars, aggressive lcs, and low to mid 70s temps for jar and sub colonization the things could be pinning in under ten days. I had low 60s to low 70s in this back bedroom and gave things an extra day here and there just to make sure things had colonized completely. And it was still really fast.
I'll break it all down in detail in a journal entry when I do jars again. But it's pretty similar to Baba's tek with his BRF/Verm mixture on top of his grains. Just I'm using poo here and then skipping the poo later.
(I like the idea of the poo being colonized beforehand)
Thanks for explaining all that. Will be looking forward to seeing the journal when you get to it.
-------------------- Seasons don't fear the reaper. Nor do the wind, the sun, or the rain.
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tree frog
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Re: Official Copelandia/Panaeolus Thread [Re: Reishiman]
#28639261 - 01/29/24 11:43 AM (3 months, 17 days ago) |
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I layered the grains on the bottom because I wanted to measure it using the jar, and because pans seem to move much faster on poo and verm than grains. So, I wanted them running on the faster spawn first, while also adjusting their metabolism from the bit of grain sugars that inevitably steamed up into the verm during the PC cycle.
Putting the poo and verm layer on top also gives you a lot of inoculation points for the shake.
And yeah, it was pretty easy to break up. Mixing the poo with the verm gives you a pretty fluffy mix. I kept this on the dry side of field capacity so it wasn't muddy in the jars too. Shook fine. Spawned fine.
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MurphSmurf
Float On



Registered: 02/08/18
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Re: Official Copelandia/Panaeolus Thread [Re: Reishiman] 6
#28639264 - 01/29/24 11:45 AM (3 months, 17 days ago) |
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Today is an AWESOME day - my first ever Pan Pins! It feels like the first time I saws pins on a BRF cake.
Nothing ground breaking to share, but maaaaaaan, I am excited.
My biggest issue has been, besides being a very novice cultivator with bad lab technique, is my lab is cold. Cubes do alright, but I am at the bottom rungs of temps, and its slooooooow as snail shiiiii.
Anyway: I was 0-3 prior this grow of Pans, which was kind of a “throw-away”, I’ve had these little shoeboxes in 2 other set-ups playing around. I was more focused on getting really clean/spore agar dishes going.
Stumbled across the JCM thread on these tubs for pans & decided to try it. One big difference is that I decided to just “float” the shoeboxes instead of putting them on a rack above the water. The reason: I didn’t have a rack. No other reason.
W/in 48hrs of building/placing into JCM tub, I have my first ever Panaeolus pins!!!!!!!
I got so excited, I built 4 more smaller versions of the tubs (put a little aquarium ceramic mushroom in with the one tub 😂 )
2024 is going to be the “Year of the Pan” for this guy!
Coming down the pipe:
Pan var….
Alabama Magic PHV Estero TTBVI MIB
Grain: millet (75%) / rye berries (25%) NSNS <-- gonna do a Jake Oncid's cold soak with this as well and see if Myc like that better, but No Soak worked fine this round.
Bulk: LMP/Coir w/ old hpoo (Thank you Baba Yaga)
JCM tub: holding strong humidity. Water temp in upper 80f, chamber in low 80f. The surface temp of the sub has been a consistent 75-77f (I couldn’t seem to get surface temps above 68f in other setups, and that was the peak - mostly in the low to mid 60s depending on ambient temp).
“Casing”: pseudo casing anyway, just threw some fine grade verm lightly on top & misted heavy. Is casing necessary?
I know these are just little pins, nothing to write home about. But in my previous attempts, I would get to here & falter: I just couldn’t get my past attempts to fruit.
I believe the failures (outside of my mistakes, which are plenty) were in large part due to my colder-than-average laboratory. Cube does okay in my environ, but Pans just couldn't pop in my environs till now!
I apologize for the longer message, but I about jumped for joy thru my tent when I spotted these little pins. So happy I almost want to cry.
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