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OfflineJrey16
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Registered: 12/19/23
Posts: 20
Last seen: 25 minutes, 55 seconds
How long would liquid culture syringes last in the fridge?
    #28622942 - 01/16/24 05:23 AM (12 days, 2 hours ago)

Still a newbie and learning mycology and never grew mushrooms before. How long would liquid culture syringes last in the fridge? It's hard for me to get them out here in the Philippines. I got my hands on three 12cc syringes (2 Golden Teachers and 1 Penis Envy) and I'm planning on doing my first grow with Golden Teacher. Do liquid culture syringes go bad? If so, how can I store them and how long will they last?


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Invisiblestockw

Registered: 08/31/21
Posts: 450
Re: How long would liquid culture syringes last in the fridge? [Re: Jrey16]
    #28622956 - 01/16/24 05:53 AM (12 days, 2 hours ago)

LC will last a really long time at room temperature so I wouldn't be too concerned about putting it in the fridge.

If you're using LC I'd imagine you're aware sterile technique is required, so stick to that when using them & your syringes will be good for a long while.

I'd recommend learning & expanding them with agar so you don't have to purchase more in the future & you can be more in control of your process.


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OfflineLand TroutM
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I'm a teapot User Gallery


Registered: 01/08/18
Posts: 3,076
Last seen: 9 hours, 55 minutes
Re: How long would liquid culture syringes last in the fridge? [Re: stockw]
    #28623231 - 01/16/24 10:12 AM (11 days, 21 hours ago)

Liquid culture seems like a really good long term storage media. I keep many cultures in liquid culture for storage, I’m not sure what my oldest cubensis would be, but it’s years. My oyster culture has been in the fridge since 2015.


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InvisibleLadysKnight
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Registered: 10/09/15
Posts: 1,655
Re: How long would liquid culture syringes last in the fridge? [Re: Land Trout]
    #28623283 - 01/16/24 10:44 AM (11 days, 21 hours ago)

I have 11yr old lc, made under non-sterile conditions. It's works well enough for pftek and agar with about 10% failure. I don't know how or why the contams stay in check, but they do. It's been kept at room temp always. I haven't made any since, but it certainly seems like a great way to preserve a culture, especially if created properly.


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OfflineJrey16
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Registered: 12/19/23
Posts: 20
Last seen: 25 minutes, 55 seconds
Re: How long would liquid culture syringes last in the fridge? [Re: LadysKnight]
    #28624142 - 01/17/24 12:32 AM (11 days, 7 hours ago)

Ok thanks y'all. So leaving the liquid in the syringe and storing the syringe in room temp in a dark place is okay? Because I saw online and on youtube people saying you have to put the liquid in a vial if you want to store it.


Edited by Jrey16 (01/17/24 12:33 AM)


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InvisibleGoatrider
Rhythm Guitarist
Male


Registered: 04/08/20
Posts: 4,396
Loc: Germany
Re: How long would liquid culture syringes last in the fridge? [Re: Jrey16]
    #28624200 - 01/17/24 05:04 AM (11 days, 2 hours ago)

Quote:

Jrey16 said:
Ok thanks y'all. So leaving the liquid in the syringe and storing the syringe in room temp in a dark place is okay? Because I saw online and on youtube people saying you have to put the liquid in a vial if you want to store it.




I guess people just quote lab standards,
and in addition they are kings of sheeple speech :stoned:
In labs they would store in darkened glass vials on a shelf,
you would store it in a syringe in a dark drawer.
No difference to me.

Just listen to the mushheads here on the Shroomery,
use YT only for music, unless it´s a channel
from some of our board members :wink:

            :cookiemonster:


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OfflineJrey16
Stranger
Registered: 12/19/23
Posts: 20
Last seen: 25 minutes, 55 seconds
Re: How long would liquid culture syringes last in the fridge? [Re: Goatrider]
    #28635589 - 01/26/24 12:41 PM (1 day, 19 hours ago)

Who's some of the board members I can check out on YT?


Edited by Jrey16 (01/26/24 12:42 PM)


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Offline2001SpaceOdyssey
Stranger
Registered: 01/26/24
Posts: 13
Last seen: 1 hour, 35 seconds
Re: How long would liquid culture syringes last in the fridge? [Re: Jrey16]
    #28636632 - 01/27/24 08:53 AM (23 hours, 6 minutes ago)

Best place is in an airtight container in the fridge


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InvisibleGoatrider
Rhythm Guitarist
Male


Registered: 04/08/20
Posts: 4,396
Loc: Germany
Re: How long would liquid culture syringes last in the fridge? [Re: Jrey16]
    #28636743 - 01/27/24 10:40 AM (21 hours, 20 minutes ago)

Quote:

Jrey16 said:
Who's some of the board members I can check out on YT?




For example:
https://www.youtube.com/@Munchauzen
https://www.youtube.com/@stipeandcap2007

        :cookiemonster:


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