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Offlinemycoamateur
Noob

Registered: 01/18/24
Posts: 1
Last seen: 1 day, 10 hours
Reuse of agar after failed germination
    #28635742 - 01/26/24 03:28 PM (1 day, 16 hours ago)

Good day all - yes I'm basically a noob and yes I read the primary intro guides and threads, including the search guide, but apparently I still suck at search.

I went multi-spore syringe to agar on 10 plates. 2 are growing mycelium, 1 was contaminated, and the remaining 7 are doing absolutely nothing and appear clean. No growth of any kind after about 4 weeks. There is also no sign of moisture (perhaps I didn't use enough MSS solution on these).

Can I simply reuse these blank plates? Or is there a reason not to do so?

Thanks for your wisdom


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InvisibleBaba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,955
Loc: Hyperspace Chicken Coop
Trusted Cultivator
Re: Reuse of agar after failed germination [Re: mycoamateur] * 3
    #28635756 - 01/26/24 03:37 PM (1 day, 16 hours ago)

Wouldn't use them as is but I have put agar from failed germination plates in with new agar and sterilized again. Not doing it on the regular but when trying to revive an old print for example it can happen that I end up with 10 or so non germinated plates.


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OfflineLand TroutM
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I'm a teapot User Gallery


Registered: 01/08/18
Posts: 3,076
Last seen: 9 hours, 55 minutes
Re: Reuse of agar after failed germination [Re: mycoamateur]
    #28635786 - 01/26/24 03:56 PM (1 day, 16 hours ago)

I would watch them good long while to be sure there’s no life forms on them, but I’ve used plates like your describing, but I think I’ve always used them with fool proof species that are very predictable like cubes and oyster, things that are tough and fast.


Edited by Land Trout (01/26/24 03:57 PM)


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OfflinemorningWood
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Registered: 12/25/23
Posts: 123
Last seen: 8 hours, 26 minutes
Re: Reuse of agar after failed germination [Re: Land Trout]
    #28635850 - 01/26/24 04:33 PM (1 day, 15 hours ago)

I've been known to reuse plates that show NO growth. You could use them to cool blades or I sometimes open them in my sab to see how funky the air is.


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OfflineBuzzPion
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Registered: 12/06/23
Posts: 43
Last seen: 14 hours, 30 minutes
Re: Reuse of agar after failed germination [Re: morningWood]
    #28636462 - 01/27/24 06:33 AM (1 day, 1 hour ago)

I have some failed plates I was looking at this morning. I will use them. Why not? If they get funky no big whoop.

Make for good practice anyway.


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