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Takeatrip
Stranger
Registered: 09/10/23
Posts: 103
Last seen: 6 hours, 42 minutes
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Do you sterilise your old plastic petri dishes?
#28631001 - 01/22/24 12:17 PM (5 days, 19 hours ago) |
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Just a quick question really. Do you guys sterilise your plastic petri dishes before reusing them or do you bin them? If you do sterilise them, do you use a pressure canner and can the dishes take the heat? I do have glass ones but I also have bought some plastic ones, stupidly I binned most as they were all contaminated due to poor cleanliness practices which I am improving on all the time. Any hints or tips would be great 👍🏻
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Goatrider
Rhythm Guitarist



Registered: 04/08/20
Posts: 4,396
Loc: Germany
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Re: Do you sterilise your old plastic petri dishes? [Re: Takeatrip] 1
#28631018 - 01/22/24 12:30 PM (5 days, 19 hours ago) |
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Standard plastic petri is for single use only. You need at least PP(#5), so polypropylen to withstand pc temps.
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WyoMX

Registered: 07/06/15
Posts: 2,100
Loc: PNW
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Re: Do you sterilise your old plastic petri dishes? [Re: Takeatrip] 1
#28631019 - 01/22/24 12:32 PM (5 days, 19 hours ago) |
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Most plastic petris's are not reusable unfortunately. Some that are PP are but those are normally not the clearest dishes. That's one thing that people are trying to figure out is to cut down on the plastic waste as some cultivators go through 500 petris in a couple months. Glass is a good alternative for sure. See alot of people turning to glass nowadays.
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cybork
Stdt mycology


Registered: 07/01/23
Posts: 146
Last seen: 47 minutes, 33 seconds
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Re: Do you sterilise your old plastic petri dishes? [Re: Takeatrip]
#28631031 - 01/22/24 12:40 PM (5 days, 19 hours ago) |
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I sterilize and reuse them.
Whether you can put them in a PC depends on the kind of plastic. Polypropylene is heat resistant enough. Other plastics might melt. PP or often PP5 (and even other PPs) is what the mark will say if it is polypropylene. If you bought petridishes prefilled with agar, and are of that very crackable plastic, I assume it is not PC-able.
I have 2 plastic kinds: ketchup thingies with air holes (covered with breathing tape) and round container lids with jars. Those latter ones are the bomb. The ketchup thingies I don't really use anymore, but you can clean, re-tape, and PC them.
I wash them with soap, let them dry, add agar in them, close them not to tight, and then PC them. Works every time. (it did took me some time to get it right but that's a different question).
PS: reusable for agar work is something different regarding it is actually safe and reusable regarding toxics. And that is also a complete other topic. Better to use glass!
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HelloImBob
Old Guy

Registered: 03/30/08
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Re: Do you sterilise your old plastic petri dishes? [Re: cybork]
#28631077 - 01/22/24 01:40 PM (5 days, 18 hours ago) |
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Quote:
cybork said: I sterilize and reuse them.
Whether you can put them in a PC depends on the kind of plastic. Polypropylene is heat resistant enough. Other plastics might melt. PP or often PP5 (and even other PPs) is what the mark will say if it is polypropylene. If you bought petridishes prefilled with agar, and are of that very crackable plastic, I assume it is not PC-able.
I have 2 plastic kinds: ketchup thingies with air holes (covered with breathing tape) and round container lids with jars. Those latter ones are the bomb. The ketchup thingies I don't really use anymore, but you can clean, re-tape, and PC them.
I wash them with soap, let them dry, add agar in them, close them not to tight, and then PC them. Works every time. (it did took me some time to get it right but that's a different question).
PS: reusable for agar work is something different regarding it is actually safe and reusable regarding toxics. And that is also a complete other topic. Better to use glass!
What do you mean by even other PPs?
PP5 is the number/symbol for polypropylene.
What other PPs are there besides the one attached to you? I would not recommend trying to sterilize that one...
-------------------- Quote from Stipe-n-Cap "You appear to be talking about boosting tryptamine content in mycelium by amending LC with....whatever your amendments are. I have to say I'm a tad disappointed that you're addressing us with the shorthand of a 13 yo girl who's texting her besty for make-up tips, instead of proper English, which causes me to have doubts."
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cybork
Stdt mycology


Registered: 07/01/23
Posts: 146
Last seen: 47 minutes, 33 seconds
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Re: Do you sterilise your old plastic petri dishes? [Re: HelloImBob]
#28631086 - 01/22/24 01:54 PM (5 days, 18 hours ago) |
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What do you mean by even other PPs?
PP5 is the number/symbol for polypropylene. >>
Sometimes the container says PP plus a number other than 5. That's what I meant with the s.
What other PPs are there besides the one attached to you? I would not recommend trying to sterilize that one... >>you are ASSUMING.
Kind regards, cybork She - his - theirs
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HelloImBob
Old Guy

Registered: 03/30/08
Posts: 219
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Re: Do you sterilise your old plastic petri dishes? [Re: cybork]
#28631209 - 01/22/24 03:42 PM (5 days, 16 hours ago) |
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Sorry I don't know what people are doing these days with themselves.
I don't understand or follow pronoun whatevers.
It was a joke and if you take offense I'm sorry I'm old I don't understand today's times.
PP will only have #5
Copied from Wikipedia
In the United States, plastic products are printed with numbers 1–7 depending on the type of resin. Type 1 (polyethylene terephthalate) is commonly found in soft drink and water bottles. Type 2 (high-density polyethylene) is found in most hard plastics such as milk jugs, laundry detergent bottles, and some dishware. Type 3 (polyvinyl chloride) includes items such as shampoo bottles, shower curtains, hula hoops, credit cards, wire jacketing, medical equipment, siding, and piping. Type 4 (low-density polyethylene) is found in shopping bags, squeezable bottles, tote bags, clothing, furniture, and carpet. Type 5 is polypropylene and makes up syrup bottles, straws, Tupperware, and some automotive parts. Type 6 is polystyrene and makes up meat trays, egg cartons, clamshell containers, and compact disc cases. Type 7 includes all other plastics such as bulletproof materials, 3- and 5-gallon water bottles, cell phone and tablet frames, safety goggles and sunglasses.[86] Having a recycling code or the chasing arrows logo on a material is not an automatic indicator that a material is recyclable but rather an explanation of what the material is. Types 1 and 2 are the most commonly recycled.
-------------------- Quote from Stipe-n-Cap "You appear to be talking about boosting tryptamine content in mycelium by amending LC with....whatever your amendments are. I have to say I'm a tad disappointed that you're addressing us with the shorthand of a 13 yo girl who's texting her besty for make-up tips, instead of proper English, which causes me to have doubts."
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Goatrider
Rhythm Guitarist



Registered: 04/08/20
Posts: 4,396
Loc: Germany
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Re: Do you sterilise your old plastic petri dishes? [Re: HelloImBob] 2
#28631493 - 01/22/24 08:49 PM (5 days, 11 hours ago) |
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People made it kind of slang here. There is no PP5. That would indicate there has to be PP1-4 also. The number is the resin identification code. #3 would be PVC f.e. Polypropylen is just PP, resin code 5 
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cybork
Stdt mycology


Registered: 07/01/23
Posts: 146
Last seen: 47 minutes, 33 seconds
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Re: Do you sterilise your old plastic petri dishes? [Re: HelloImBob]
#28631567 - 01/22/24 10:49 PM (5 days, 9 hours ago) |
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No problem, I was trying to make a joke to a joke. I'm not offended and was only making a joke to the crazy world we live in today. In some way that happened to me not so long ago at work; on an email I replied referring to someone in the thread as him, and he apparantly was a she. It was an unintended mistake. The foreign name really appeared to me as male-ish. Anyways, that got a trail. Yes, seriously. And btw it was a very decent email about some settings in a database. Nothing with puns or something. Not trying to offend you or anyone by the way. Please don't cancel me.
Regarding PP-five: if it has 5 within that that triangle recycle symbol ♻️, that means the plastic is PP.
Sometimes a producer places PP and some other number. For one of their own benefits. Doesn't haappen that much, but sometimes one sees it.
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,310
Loc: The Inexpressible...
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Re: Do you sterilise your old plastic petri dishes? [Re: Takeatrip]
#28631572 - 01/22/24 11:01 PM (5 days, 8 hours ago) |
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I use empty plastic petri dishes for making spore syringes. I just rinse the inside with hot water then carefully drain the water out then reuse for the next spore syringe.
As for agar dishes, i probably wouldnt bother cleaning them up unless i was real desperate for a petri dish.
-------------------- "What you must understand is that your physical dimension affects everyone in the higher dimensions as well. All things are interconnected. All things are One. Therefore, if one dimension is broken or out of balance, then all other dimensions will experience repercussions." - Pleiadian Prophecy 2020 The New Golden Age by James Carwin PROJECT BLUE BOOK ANALYSIS! (312 pages!) | Psychedelics & UFOs | Ready to Contact UFOs? | The Source on Mushrooms | Trippy Gematrix | Dj TeknoLogical | Fentanyl Test Kits R.I.P. Big Worm || The Start of the Ascension Process was 2020. Welcome to the Next Great Era of Earth 🌎🌍🌏
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EnDelfisE
Rider On The Storm


Registered: 09/07/12
Posts: 499
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Re: Do you sterilise your old plastic petri dishes? [Re: LogicaL Chaos]
#28635494 - 01/26/24 10:36 AM (1 day, 21 hours ago) |
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Hey anyone has experience using uvc lamp that produces ozone to sterilize standard no high temp resistance for pc, Agar plates?
I used this cheap 35 eu, ( supposedly on discount lol) uvc ozone remote control lamp to kill some fruit flies taking over some bulk when I was away for some days, works great but you need to be carefully not to be exposed to the ozone, I leave the house and come back to open the balcony doors real fast and leave again for an hour, that's for a big room, the ozobes snell btw omg vabt stand it
I was thinking to try it onr the used plates, put them in a big sab and run the uvc ozone near it, I think uvc can deteriorate some plastics tho, depended on exposure, but I've seen uvc labeled Agar plates at lab supplier store before.
Has anyone ever researched experimented with it? 🐰
Edited by EnDelfisE (01/26/24 10:39 AM)
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AlexandrDughin
Explorer

Registered: 10/30/23
Posts: 35
Loc: Europe
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Re: Do you sterilise your old plastic petri dishes? [Re: EnDelfisE] 1
#28635545 - 01/26/24 11:43 AM (1 day, 20 hours ago) |
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Unfortunately i am one of those " desperate for a petri dish" as someone here said.
What i do is : - wash with soap and hot water - quick dip in boiling water ( 15-30 seconds ) - let them drain face down in a box (sab) - turn them up in the sab spray alcohol ( 70 % ) - close them
how efficient it is ? idk really, kinda end up with 3/10 contaminated and 7/10 clean after i pour agar into them
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