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Offlinemycoloking
Mycologist Newbie


Registered: 01/01/24
Posts: 46
Last seen: 2 days, 13 hours
Regrouping My Mycology Process * 1
    #28631368 - 01/22/24 06:38 PM (5 days, 13 hours ago)

Update...
Had to make some adjustments to my process here...I have way too much contamination going on.:eek: I also think my impatience isn't helping anything.

I have a couple other posts going on my progress as a rookie mycologist...:mushroom2:
How does this look getting ready for -FRUITING-
Pictures Of Colonizing Grains
Long Grain Parboild Rice
Photos through jars POPCORN

SO I REGROUPED..:alert:
I went through and removed all contaminated jars also a good scrub and cleaning of my area. THen... :rolljoint:

I am a rookie at this and of course did a lot of reading ahead. Watched the youtube videos and whatnot. I got my hands on a spore syringe and it was on.
Now in hindsight I notice patients is a must! Cleanliness against contamination...a must! Trial by error, a must!

Fast forward...I have some LC cultures I was able to make from B+ spore syringe vendor. I also, tried inoculating spawn brown rice jars and some Uncle bens Instant rice paks. Hindsight, I was trying to inoculate my jars using the oven tek method...bad decision as I used this method in both of my inoculation sessions. From my results probably a bad choice! So I pulled me SAB together for future sessions.

I have also worked on my tek for preparing my rice spawn grains, which will still need some experiance and work at, but I am getting better. Also working on different LC culture recipes. I managed to get a good process for AGAR to start to use in my processes. I was suggested so I decided I needed to jump into getting used to using it. Found a kewl Potato Dextros Agar recipe. Which I was excited worked well on my first attempt at making it! Hurrah!
Pictures of my successful AGAR dishes :petri: :wink:


Was kinda tricky pouring in my SAB but it worked!

Now...I can pull from my original LC from my vendor spore syringe...and actually see what I have here...

What methods do you guys use to get a LC onto AGAR??


--------------------
Hoping to grow some great mushies with some TLC, Tender Loving Care!:thumbup:


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OfflineMwj12977
OLD DAD
 User Gallery

Registered: 12/12/23
Posts: 76
Last seen: 10 minutes, 52 seconds
Re: Regrouping My Mycology Process [Re: mycoloking] * 1
    #28631715 - 01/23/24 05:20 AM (5 days, 2 hours ago)

Agitate the culture and suck some up into a clean syringe. Put a drop on the dish.


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Offlinemycoloking
Mycologist Newbie


Registered: 01/01/24
Posts: 46
Last seen: 2 days, 13 hours
Re: Regrouping My Mycology Process [Re: Mwj12977]
    #28631826 - 01/23/24 08:03 AM (4 days, 23 hours ago)

Quote:

Mwj12977 said:
Agitate the culture and suck some up into a clean syringe. Put a drop on the dish.



Thanks, I was thinking that was enough. I appreciate the reply. I have some LC to get onto AGAR :smile:


--------------------
Hoping to grow some great mushies with some TLC, Tender Loving Care!:thumbup:


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Invisibleelasticaltiger
Like Tigers in Coitus
Male


Folding@home Statistics
Registered: 06/24/13
Posts: 8,042
Trusted Cultivator
Re: Regrouping My Mycology Process [Re: mycoloking]
    #28631900 - 01/23/24 09:15 AM (4 days, 22 hours ago)

How are you prepping your "rice spawn grain" ?


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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Offlinemycoloking
Mycologist Newbie


Registered: 01/01/24
Posts: 46
Last seen: 2 days, 13 hours
Re: Regrouping My Mycology Process [Re: elasticaltiger] * 1
    #28632696 - 01/23/24 10:17 PM (4 days, 9 hours ago)

Quote:

elasticaltiger said:
How are you prepping your "rice spawn grain" ?




Currently I wash/rinse rice, add rice to already boiling pot of water, boil for around 17-20mins. Drain and rinse with cold water, place on flat surface to air dry for about 1-2hrs. Jar it up and PC for 120mins, cool in pot for 30mins and then remove.

How do you do yours?


--------------------
Hoping to grow some great mushies with some TLC, Tender Loving Care!:thumbup:


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Invisibleelasticaltiger
Like Tigers in Coitus
Male


Folding@home Statistics
Registered: 06/24/13
Posts: 8,042
Trusted Cultivator
Re: Regrouping My Mycology Process [Re: mycoloking]
    #28632755 - 01/24/24 01:37 AM (4 days, 6 hours ago)

Quote:

mycoloking said:
Quote:

elasticaltiger said:
How are you prepping your "rice spawn grain" ?




Currently I wash/rinse rice, add rice to already boiling pot of water, boil for around 17-20mins. Drain and rinse with cold water, place on flat surface to air dry for about 1-2hrs. Jar it up and PC for 120mins, cool in pot for 30mins and then remove.

How do you do yours?



I don't use rice beyond grinding for flour. It's just always seemed like it would be really easy to have the grains become too mushy. I'm kind of amazed you're able to boil it for so long.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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OfflineLogicaL ChaosM
Ascension Energy & Alien UFOs
Male User Gallery


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Registered: 05/12/07
Posts: 69,310
Loc: The Inexpressible...
Last seen: 1 hour, 29 minutes
Trusted Cultivator
Re: Regrouping My Mycology Process [Re: mycoloking]
    #28632762 - 01/24/24 01:45 AM (4 days, 6 hours ago)

Quote:

mycoloking said:
Quote:

elasticaltiger said:
How are you prepping your "rice spawn grain" ?




Currently I wash/rinse rice, add rice to already boiling pot of water, boil for around 17-20mins. Drain and rinse with cold water, place on flat surface to air dry for about 1-2hrs. Jar it up and PC for 120mins, cool in pot for 30mins and then remove.

How do you do yours?




Quite similar to grain prep. Very nice :yesnod:

Someday I wanna try wild rice. I think it would look cool. :cool2:

As for transferring LC to agar, yeah use a clean, empty, new spore syringe (blunt-tip syringe). Or you can also use a new long glass eye dropper or glass pippette.


--------------------
"What you must understand is that your physical dimension affects everyone in the higher dimensions as well. All things are interconnected. All things are One. Therefore, if one dimension is broken or out of balance, then all other dimensions will experience repercussions." - Pleiadian Prophecy 2020 The New Golden Age by James Carwin

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Offlinemycoloking
Mycologist Newbie


Registered: 01/01/24
Posts: 46
Last seen: 2 days, 13 hours
Re: Regrouping My Mycology Process [Re: elasticaltiger]
    #28634775 - 01/25/24 05:58 PM (2 days, 13 hours ago)

Quote:

elasticaltiger said:
Quote:

mycoloking said:
Quote:

elasticaltiger said:
How are you prepping your "rice spawn grain" ?




Currently I wash/rinse rice, add rice to already boiling pot of water, boil for around 17-20mins. Drain and rinse with cold water, place on flat surface to air dry for about 1-2hrs. Jar it up and PC for 120mins, cool in pot for 30mins and then remove.

How do you do yours?



I don't use rice beyond grinding for flour. It's just always seemed like it would be really easy to have the grains become too mushy. I'm kind of amazed you're able to boil it for so long.





I am actually thinking of switching to whole oat grains...I can get a 50# bag at Tractor Supply for like 27$ and that would last me quite a while. We will see. If you cook it correctly, the rice actually doesn't turn out mushy when done correctly. But I want to make my way through all the grains until I find one that works well for me  :smile:


--------------------
Hoping to grow some great mushies with some TLC, Tender Loving Care!:thumbup:


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Offlinemycoloking
Mycologist Newbie


Registered: 01/01/24
Posts: 46
Last seen: 2 days, 13 hours
Re: Regrouping My Mycology Process [Re: LogicaL Chaos]
    #28634777 - 01/25/24 06:01 PM (2 days, 13 hours ago)

Quote:

LogicaL Chaos said:
Quote:

mycoloking said:
Quote:

elasticaltiger said:
How are you prepping your "rice spawn grain" ?




Currently I wash/rinse rice, add rice to already boiling pot of water, boil for around 17-20mins. Drain and rinse with cold water, place on flat surface to air dry for about 1-2hrs. Jar it up and PC for 120mins, cool in pot for 30mins and then remove.

How do you do yours?




Quite similar to grain prep. Very nice :yesnod:

Someday I wanna try wild rice. I think it would look cool. :cool2:

As for transferring LC to agar, yeah use a clean, empty, new spore syringe (blunt-tip syringe). Or you can also use a new long glass eye dropper or glass pippette.




THanks for the tip :smile: Yeah its pretty simple..tried soaking it overnight for another bacth of grains, tonight, the only difference..we will see how those do! I agree on the wild rice thing..it would be cool to watch colonize :wink:


--------------------
Hoping to grow some great mushies with some TLC, Tender Loving Care!:thumbup:


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Offlinemycoloking
Mycologist Newbie


Registered: 01/01/24
Posts: 46
Last seen: 2 days, 13 hours
Re: Regrouping My Mycology Process [Re: mycoloking] * 1
    #28634794 - 01/25/24 06:15 PM (2 days, 13 hours ago)

Seems as though my new LC syringe from Myyco, shout out to them, is going great! Great product, WOW! I enjoyed getting it! Anyways, great all the way around!Very Pleased!



Was able to make a LC Jar and some others.
Seems to be vigorous! Just after three days.


I even through some to AGAR to see how it grows out :wink: all for educational purposes;);)


--------------------
Hoping to grow some great mushies with some TLC, Tender Loving Care!:thumbup:


Edited by mycoloking (01/25/24 06:17 PM)


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Offlinemycoloking
Mycologist Newbie


Registered: 01/01/24
Posts: 46
Last seen: 2 days, 13 hours
Re: Regrouping My Mycology Process [Re: LogicaL Chaos]
    #28634813 - 01/25/24 06:27 PM (2 days, 13 hours ago)

I am not happy with my B+ spore syringe that I started all of this journey with at first....I am working on narrowing this one down with AGAR. I have a few plates that I am trying to get to LC Jar that I can spawn with. Sad when the first strain you wanted to start with isn't going as planned..maybe it was contam....??

Anyway I tossed all but one LC Jar and One popcorn jar that looks to have some viable mycelium I can throw to AGAR and narrow a good culprit. Fingers crossed.

I didnt start off with AGAR, but I am glad I have started the AGAR journey :wink:


--------------------
Hoping to grow some great mushies with some TLC, Tender Loving Care!:thumbup:


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