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Offline3.A.M
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Re: Identidying Contamination On My Agar Plates (Condensations, polyfill, micropore tape) [Re: Screwup] * 1
    #28635219 - 01/26/24 04:40 AM (1 day, 17 hours ago)

:
Quote:

Screwup said:
Bump for you cause I’m too lazy to help



:rofl:

Condensation on grain jars can potentially mean contamination but not on agar, simple environmental temp changes can cause that, don’t use poly fill for plates, there should be enough oxygen in a plate to see you through plenty of growth when sealed,  as to where and if to transfer? Sometimes it’s not obvious but fast, consistent, clean growth is always desirable, if you’re uncertain you can always transfer an ideal candidate to water agar or brf puck to clean it up and be certain, which I would definitely do with what you have there.
Also, micropore is a bitch (been there, done that) try either cling film cut down to size or even better, grafting tape 👍


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