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OfflineDarcness
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Is my 1st LC go a bust?
    #28633379 - 01/24/24 02:01 PM (11 months, 17 days ago)

So I bought an already to go LC kit nocked it up with some GT spores on the 17th and I think maybe I caught a contamination or perhaps it’s supposed to look like this, maybe someone with a better eye can tell. When I gave it a little stir it looks like 3 little cotton balls rolling around




Edited to put under subcategory

Edited by Darcness (01/24/24 02:15 PM)

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OfflineMrJong
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Re: Is my 1st LC go a bust? [Re: Darcness] * 1
    #28633405 - 01/24/24 02:25 PM (11 months, 17 days ago)

Looks about right to me, can't know for sure what you have in there without testing on agar. Wouldn't recommend spores to anything else than agar in general besides maybe PF tek.

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InvisibleLadysKnight
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Re: Is my 1st LC go a bust? [Re: MrJong] * 1
    #28633415 - 01/24/24 02:38 PM (11 months, 17 days ago)

You contaminated it when you inserted spores. Ain't no clean spores


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OfflineHelloImBob
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Re: Is my 1st LC go a bust? [Re: LadysKnight]
    #28633451 - 01/24/24 03:19 PM (11 months, 17 days ago)

I wouldn't say that it can't be done with spores, it can be and has been done in the past plenty but your odds decrease in it being clean the more spores you inject and obviously depending on how clean the spores were collected.

I'm old people used to do it all the time with decent results but it's not recommended these days.

You could test it on a small pf jar or something if you don't have agar but beware any time that you stick a needle into the jar you risk contamination if done incorrectly.

It looks nice and clean to be but you always have to test.

Get some extra syringes and needles to save the LC in without reinserting a used syringe and best practice is usually to first inject a new LC jar before anything else to continue your LC in case your first jar has any issues later.


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Quote from Stipe-n-Cap

"You appear to be talking about boosting tryptamine content in mycelium by amending LC with....whatever your amendments are. I have to say I'm a tad disappointed that you're addressing us with the shorthand of a 13 yo girl who's texting her besty for make-up tips, instead of proper English, which causes me to have doubts."

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OfflineDarcness
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Re: Is my 1st LC go a bust? [Re: HelloImBob]
    #28633498 - 01/24/24 04:19 PM (11 months, 17 days ago)

Oh I wasn’t aware things are done differently now, it’s been over 13 years since I last messed around with mycology and kinda just bought stuff and went at it. I got some BRF jars and since have plenty of new syringes my plan was to use LC. Guess I’ll have to learn what agar is and if I see if I screwed the pooch on my LC

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OfflineDarcness
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Re: Is my 1st LC go a bust? [Re: Darcness]
    #28635437 - 01/26/24 09:45 AM (11 months, 15 days ago)

So it’s been 9 days since inoculation of my LC I seems to have grown a bunch since first pic posted I’ll be sure to take a pic when I can and post it, I was wondering should I keep giving the jar a stir every couple days and how long should I let it grow before using/store in the fridge

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OfflineDarcness
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Re: Is my 1st LC go a bust? [Re: Darcness]
    #28635812 - 01/26/24 04:15 PM (11 months, 15 days ago)

Here are some pics taken today (day 9) of it sitting still and after I gave it a spin





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OfflineDarcness
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Re: Is my 1st LC go a bust? [Re: Darcness]
    #28635817 - 01/26/24 04:17 PM (11 months, 15 days ago)

Is this ready to use or do I have to wait still? If it is ready can I use it and still leave it out growing longer?

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OnlinemorningWood
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Re: Is my 1st LC go a bust? [Re: Darcness]
    #28635837 - 01/26/24 04:27 PM (11 months, 15 days ago)

It's ready to TEST on agar. It will continue to grow in lc, you have a lot of room in the jar still.


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OfflineDarcness
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Re: Is my 1st LC go a bust? [Re: morningWood]
    #28645551 - 02/03/24 06:39 PM (11 months, 7 days ago)

So when I was pulling LC from my jar it was a pain so I opened the lid filled my syringes and put it back to keep growing, so it’s clearly growing as there wasn’t really any mycelium left after filling syringes but I noticed this growing/floating on top and not sure if it’s normal or a contamination anyone have thoughts

And here is a pic of what’s still growing on bottom

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Offlinefungalfrontier
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Re: Is my 1st LC go a bust? [Re: Darcness]
    #28645574 - 02/03/24 07:02 PM (11 months, 7 days ago)

I would say that is contamination. I made the same mistake , blasting spores into lc. 3/4 seemed OK but one had a piece exactly like that and everything (I mean it 100%) of everything I inoculated with that lc grew green mold. I later tested the mss and it was contaminated to the fullest extent.

I diluted mss way down and am hoping to maybe get some clean growth on a plate to save the variety.

Save yourself and your wallet and get rid of that lc. Learn about agar, and your life will get much easier as far as this goes.

I also wouldn't recommend opening a sterile jar unless in front of a flowhood. That's risky business.


Take everything I say with a grain of salt though,  I'm a noob. Might wait for someone a little more experienced to give you a definite answer.


Also just to share my experience. Buying pre sterilized stuff doesn't seem to be cost effective to me. I forked out a bunch on all in one bags and pre sterilized spawn just to end up buying a presto anyways.



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***🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿***




Edited by fungalfrontier (02/03/24 07:13 PM)

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OfflineDarcness
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Re: Is my 1st LC go a bust? [Re: fungalfrontier]
    #28645598 - 02/03/24 07:17 PM (11 months, 7 days ago)

The funny part is that white part that is floating has what appears to be mycelium growing under it, looks just like the rest of what’s been growing the entire time

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OfflineA.k.aM
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Re: Is my 1st LC go a bust? [Re: Darcness]
    #28645609 - 02/03/24 07:24 PM (11 months, 7 days ago)

That looks normal but you can’t really tell by looking. I’d say there’s no bacteria though cuz the liquid is clear.

You need to break up the myc more as it grows so it doesn’t clog the needle too, opening the jar to use it was a huge risk especially if you don’t have a flowhood.


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LAGM2020

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OfflineDarcness
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Re: Is my 1st LC go a bust? [Re: A.k.a]
    #28645648 - 02/03/24 07:47 PM (11 months, 7 days ago)

I did open it in my SAB but I still know that’s not 100% safe at all but I tried my best to keep everything clean

And i opened it because I was having trouble filling the syringes even after breaking everything up

Edited by Darcness (02/03/24 07:48 PM)

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Offlinefungalfrontier
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Re: Is my 1st LC go a bust? [Re: Darcness]
    #28645677 - 02/03/24 08:12 PM (11 months, 7 days ago)

Most people recommend putting some sort of agitator for that reason. Have to keep the mycelium broke up or use a fat gauge needle. That should help it grow faster too with more inoculation points.

As for that growth looking "normal" yes it looks similar to what we want , but I'd question why that piece is floating vs suspended like all the other mycelium.

Someone just told me yesterday when I was asking about my agar plates that "uniformity is key". Anything that looks out of place probably is.


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OfflineDarcness
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Re: Is my 1st LC go a bust? [Re: fungalfrontier]
    #28645682 - 02/03/24 08:16 PM (11 months, 7 days ago)

Well I do have a magnetic agitator in the bottom and I’ve seen some people mention that after a while LC will grow so much that it covers the top of the liquid and prevents itself getting oxygen and stalls out and since I don’t have experience with LC I wasn’t sure if that is what was happening here or not

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InvisibleSeventhMushroom
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Registered: 12/30/22
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Re: Is my 1st LC go a bust? [Re: Darcness]
    #28645708 - 02/03/24 08:46 PM (11 months, 7 days ago)

Quote:

Darcness said:
Well I do have a magnetic agitator in the bottom and I’ve seen some people mention that after a while LC will grow so much that it covers the top of the liquid and prevents itself getting oxygen and stalls out and since I don’t have experience with LC I wasn’t sure if that is what was happening here or not





Typically, my LC mycelium starts "floating" and growing across the surface like that only after the rest of the liquid is full of mycelium. Otherwise, the stuff just slowly sinks to the bottom before it has a chance to grow enough to float from surface tension. And, if left undisturbed, I get the "little cotton balls" as you mentioned that roll around on the bottom. But not floating on the top: not at first, and not without the rest of the jar being colonized.

Also, it's really hard to see anything in your pictures, because it's all out of focus: your camera seems to be focusing on the glass itself, rather than the floating goodies *inside* the jar. Hard to say anything without seeing a good picture. I know it's a challenge.


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LAGM 2024

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OfflineDarcness
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Re: Is my 1st LC go a bust? [Re: SeventhMushroom]
    #28645805 - 02/03/24 10:12 PM (11 months, 7 days ago)



Best shots I could take from the top and one from side showing how its growing under it hope they help

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OfflineA.k.aM
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Re: Is my 1st LC go a bust? [Re: Darcness]
    #28645978 - 02/04/24 05:03 AM (11 months, 6 days ago)

Yeah it’s normal, if you don’t shake them up regularly they’ll form a mat on the surface and start pinning. Kinda cool actually but makes the lc useless.

Try swirling the jar really fast and then suddenly switch directions, usually it’s enough to bust up clumps without getting the filter wet.


--------------------
LAGM2020

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OfflineDarcness
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Re: Is my 1st LC go a bust? [Re: A.k.a]
    #28646426 - 02/04/24 01:27 PM (11 months, 6 days ago)

Well thank you very much i did what you said and it broke everything up really nice appreciate it

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