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OfflineSupaThaRipper
Genetics Hoarder
I'm a teapot User Gallery


Registered: 09/02/13
Posts: 1,488
Loc: USA Flag
Last seen: 1 hour, 42 minutes
Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Baba Yaga]
    #28631714 - 01/23/24 05:19 AM (4 days, 17 hours ago)

Ahh yes. I think this is also referred to as a slurry? For when theyโ€™re going on a walk and inoculating the world lol..

Thanks guys


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InvisibleReverendMyc

Registered: 03/29/19
Posts: 1,494
Re: Official Ps. Subtropicalis/Semperviva Thread [Re: SupaThaRipper] * 3
    #28631957 - 01/23/24 10:07 AM (4 days, 13 hours ago)

I have a write up in the works about LI and Subtropicalis was my first success with many jars since.
Quote:

ReverendMyc said:
How I do long term culture storage in LI. Basically blenderless li.

I love LI and have great success with it with subtropicalis. I combine the ideas of LI and Long Term Sterile Water Storage and can just pour a super speedy jar of reliable culture any time I like.

This is how I perpetuate all of my favorite cultures:
  • I take a very clean plate of a culture I want to preserve and propagate.
  • Dice up the colonized part really fine (~1cm cubes) when is reaches about 75% coverage.
  • Drop the finely diced myceliated agar into media bottles that have been half filled with distilled water and sterilized.
  • Shake the bottles once a day or month for as long as you want to, or don't. Doesn't seem to matter.
  • Then shake vigorously and pour 5 to 10 ml into prepared grain any time after the first week.
  • When you get low, put a drop or two on a couple of new plates and start over.


Infinite-ish culture. Cheap and easy, except for the media bottles. I have been getting cheap 100 ml media bottles on the 'zon.

I usually do two bottles per culture. It would be wise to reserve one as a "Master" to keep the other going as near perpetual "slaves" in the same way they do with slants.


Some details on where this started.
LC from LI trial
Didn't really become an LC but it led to this idea when combined with the cryo vial storage in sterile dh2o threads.

Now each culture that I want to preserve gets a 100 ml media bottle with 50 ml sterilized distilled water and a whole petri dish of clean culture on finely diced agar.

What I have in LI storage (complete):
  • SWPE - A potent non-blobbing lineage from one of my first two grows
  • Tampanensis (Pollock) -Ambrose
  • Subtropicalis - "fast" -Melgo
  • Lion's mane - My first gourmet lineage -sponsor


What I am currently working on to get into storage:
  • Jack Frost -labar
  • Starry Night APE -labar
  • Pan Bisporus -sponsor
  • Pan Cyan BVI -sponsor
  • Pan Cambo Komodo -PsiloPsychin
  • Pan Cyan Kona Hi Rainbow Eucalyptus -PsiloPsychin
  • Pan Cyan Brooks FL -PsiloPsychin
  • Pan Cyan Wild Coast Africa -PsiloPsychin
  • Pan Cyan Bunnell FL -PsiloPsychin
  • Mexicana Chicon Nando -PsiloPsychin
  • Subtropicalis Bell Cap -RockinRobot
  • Subtropicalis "slow" -0t0lerance-YoshiTrainer
  • Subtrobicalis "slow" -Hindsight-YoshiTrainer
  • Subtropicalis Xica -YoshiTrainer
  • Cube Golden Teacher -Womble






--------------------
LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.
Don't Panic




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InvisibleNerex
Stranger

Registered: 02/06/22
Posts: 176
Re: Official Ps. Subtropicalis/Semperviva Thread [Re: nosf3r4tu]
    #28632157 - 01/23/24 01:41 PM (4 days, 9 hours ago)

So guys, I got my first shoebox of Semperviva contamined and now I'm sterilizing 2 jars of millet... fortunately I got these 3 plates that I'm conserving in the fridge; do you think they look well enough? Is that blue color normal while keeping them in the fridge?



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InvisibleReverendMyc

Registered: 03/29/19
Posts: 1,494
Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Nerex]
    #28632191 - 01/23/24 02:47 PM (4 days, 8 hours ago)

Normal? I have never seen a subtrop plate blue like that, but I have seen the myc get really blue on shaken grains. So I wouldn't be too scared of it. It could have just been stressed by drying or the cold. But I would give preferential treatment to the other two less blue plates and be sure to get transfers before going to grain to keep working it in any case.


--------------------
LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.
Don't Panic




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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: SupaThaRipper] * 1
    #28632506 - 01/23/24 07:13 PM (4 days, 4 hours ago)

Quote:

SupaThaRipper said:
Quote:

RockinRobot said:

Mine always recovered within 24 hours. Never used LI as I personally don't like the idea behind it. I prefer to make LC with Josex Poke and inoculate with it.




Whatโ€™s the difference between LI and LC? I always thought they were the same thing ๐Ÿ˜‚



Li is pulverized myc suspended in water. An lc is myc growing through a nutritious solution.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineSupaThaRipper
Genetics Hoarder
I'm a teapot User Gallery


Registered: 09/02/13
Posts: 1,488
Loc: USA
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: ReverendMyc]
    #28632517 - 01/23/24 07:21 PM (4 days, 3 hours ago)

Wow Iโ€™m liking the sound of this! Think Iโ€™ll give her a go myself ๐Ÿ˜„ Also wanna noc up a few places outside as well anyways. Seems like a good fit ๐Ÿซ 


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OfflineYoshiTrainer
Onion tied to belt
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: SupaThaRipper] * 10
    #28632617 - 01/23/24 08:38 PM (4 days, 2 hours ago)

Xico on RGS.



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OfflineLuckyDuck88
Fungus Nerd
Male

Registered: 12/14/23
Posts: 3
Loc: ND
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: YoshiTrainer] * 1
    #28632743 - 01/24/24 12:52 AM (3 days, 22 hours ago)

Fast variety is fully colonized on oats and ready to go to tub, currently cleaning up slow variety on agar. Loving how aggressive the mycelium has been.
Just running a simple coco/verm substrate, is there anything i need to know before i start my tubs? Just run the substrate a little dryer than cubes?


--------------------


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Offlinechris77
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: YoshiTrainer] * 1
    #28632770 - 01/24/24 02:22 AM (3 days, 20 hours ago)

Quote:

YoshiTrainer said:
Xico on RGS.






:shaqwiggle:


--------------------
the observer is the observed
            j. krishnamurti


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InvisibleSwabMarleyS
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Registered: 12/07/20
Posts: 1,450
Loc: Drunken Stupor
Re: Official Ps. Subtropicalis/Semperviva Thread [Re: YoshiTrainer] * 1
    #28632794 - 01/24/24 03:19 AM (3 days, 19 hours ago)

Quote:

YoshiTrainer said:
Xico on RGS.





Very photogenic! Some nice looking growth there!


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OfflineYoshiTrainer
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: SwabMarley]
    #28633056 - 01/24/24 09:03 AM (3 days, 14 hours ago)

Thank you guys, fingers crossed!


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OfflineSirPsycho
Purple Belt in Google-Fu
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: YoshiTrainer] * 13
    #28633091 - 01/24/24 09:31 AM (3 days, 13 hours ago)



Bottles doing bottle stuff


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  ๐Ÿ…‘๐Ÿ…ž๐Ÿ…ฃ๐Ÿ…ฃ๐Ÿ…›๐Ÿ…” ๐Ÿ…–๐Ÿ…๐Ÿ…๐Ÿ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023


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InvisibleReverendMyc

Registered: 03/29/19
Posts: 1,494
Re: Official Ps. Subtropicalis/Semperviva Thread [Re: LuckyDuck88]
    #28633157 - 01/24/24 10:38 AM (3 days, 12 hours ago)

Quote:

LuckyDuck88 said:
Fast variety is fully colonized on oats and ready to go to tub, currently cleaning up slow variety on agar. Loving how aggressive the mycelium has been.
Just running a simple coco/verm substrate, is there anything i need to know before i start my tubs? Just run the substrate a little dryer than cubes?



Sounds like you are starting off right. In my experience they do best when I start the sub dryer than cubes, thin casing once there is a good amount of myc visible on the surface (not necessarily fully colonized, though), mist the casing well one time after application, then keep them well misted after pins show up. They like lots of fae once they are pinning, but that may mean more frequent misting depending on environment.


--------------------
LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.
Don't Panic




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InvisibleTheAtlanteanS
Forever Grateful


Registered: 06/25/18
Posts: 999
Loc: Milkyway Galaxy, Laniakea Supe...
Re: Official Ps. Subtropicalis/Semperviva Thread [Re: ReverendMyc] * 8
    #28633234 - 01/24/24 12:12 PM (3 days, 11 hours ago)

Check out the growth on this plate

:shaqwiggle:




--------------------

Meditation is passive learning. Tabernacling with the one infinite Creator is passive learning. It is the learning from listening to the voice one cannot hear which answers questions which one cannot ask. The process cannot be known and cannot come into consciousness in local sense, yet the learning is there. And the strength of spirit and will which comes from this learning is helpful.
.

:egyptian:www.templeofhermes.org:egyptian:


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InvisibleMojaveMyc
Fractal Farmer
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Registered: 05/08/21
Posts: 655
Loc: Colorado
Re: Official Ps. Subtropicalis/Semperviva Thread [Re: TheAtlantean]
    #28633357 - 01/24/24 01:48 PM (3 days, 9 hours ago)

Noice!


--------------------


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OfflinePnin
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Registered: 07/18/23
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: MojaveMyc] * 4
    #28633452 - 01/24/24 03:21 PM (3 days, 7 hours ago)



--------------------
๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ
>my end of 2023 grow journal<


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OfflineBra
grow panaeolus, it's easy

Registered: 07/12/19
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Loc: Europe
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: TheAtlantean] * 10
    #28633503 - 01/24/24 04:24 PM (3 days, 6 hours ago)


Ps. Semperviva (Hoogshagenii var. convexa).
I can't wait to trip on them! :mushroom2:
I hope in about 2 or 3 weeks I'll have a chance to trip and to describe my experience.
If the next flush appears, gotta try to mist them to get some prints.


--------------------
Bra's spores giveaway


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Offlinetryptkaloids
Learner
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Bra]
    #28633553 - 01/24/24 05:03 PM (3 days, 6 hours ago)

Can I see some pics of clean spawn? I'm an exotics noob


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Invisiblethirdeyewild
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: nosf3r4tu] * 11
    #28633562 - 01/24/24 05:08 PM (3 days, 6 hours ago)



--------------------


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InvisibleReverendMyc

Registered: 03/29/19
Posts: 1,494
Re: Official Ps. Subtropicalis/Semperviva Thread [Re: tryptkaloids] * 7
    #28633590 - 01/24/24 05:26 PM (3 days, 5 hours ago)

Quote:

tryptkaloids said:
Can I see some pics of clean spawn? I'm an exotics noob




15 days old at time of this picture. 1 pint millet from li.


9 day old same li on quarts of wheat.


64 days old from li on wheat. Not as pretty. The one of left is not 100% clean. Post shake and a half hour later when the bruising got quite visible.


These fellas became this. Spawned on day 20, first harvest day 29 from spawn.


--------------------
LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.
Don't Panic




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