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SWBZA
SV-10


Registered: 05/09/23
Posts: 81
Last seen: 14 minutes, 43 seconds
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LC stalled because all the nutes have been eaten?
#28631623 - 01/23/24 01:05 AM (5 days, 6 hours ago) |
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Hi all.
Firstly, on a different thread there was a pointer to "the agar thread" with a request to the poster to go there for questions regarding agar. Is there a "liquid culture thread" where I should be asking these LC questions?
Okay, here goes. This is a hobby for me and my fun consists as much from messing around and learning as actually following a tek and growing shrooms. I've got several buckets / tubs / shoeboxes growing successfully due to following good teks, so this is one of those "how does this work", fun questions (for me, anyway). I've been playing around with LCs and on some of the higher concentration ones (2%, even 4%), the mycelium cloud seems to grow to about halfway the volume of the liquid. This one is made with 0.1% LME, just for the heck of it:

It seems to have stalled, and you can see there is only a small cloud of myc at the bottom. Same genetics as for some of my 1% and higher experiments, so the obvious question: did the myc stopped growing because the nutrients have all been utilised?
Kind regards
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Screwup
Googles your dumb questions


Registered: 01/27/22
Posts: 6,296
Last seen: 5 minutes, 47 seconds
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Re: LC stalled because all the nutes have been eaten? [Re: SWBZA]
#28631634 - 01/23/24 01:31 AM (5 days, 6 hours ago) |
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No the myc has likely stalled because you havenβt swirled it or contamination.
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SWBZA
SV-10


Registered: 05/09/23
Posts: 81
Last seen: 14 minutes, 43 seconds
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Re: LC stalled because all the nutes have been eaten? [Re: Screwup]
#28631651 - 01/23/24 02:13 AM (5 days, 5 hours ago) |
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Quote:
Screwup said: No the myc has likely stalled because you havenβt swirled it or contamination.
Most certainly stirred it, so guess it's time to take to agar to test for those contams. Happy to say making new agar (precisely for this and three other LCs I want to test) was on my schedule for today already 
On stirring: I find different opinions, such as:
- After inoculation, let stand for a week before stirring
- Only stir every other day (or some other frequency) to allow myc to recover
- Don't stir fast enough to form a vortex
- Every so often, stir fast enough to form a vortex so 1) you break up the myc; 2) you oxygenate the liquid
- Set your magnetic stirrer on slow and leave your LC there until you want to use it
Very confusing for a newbie. For this particular LC, I stirred for around 3 hours at a time, and then rested it, in a 24 hour cycle.
Any thoughts?
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Screwup
Googles your dumb questions


Registered: 01/27/22
Posts: 6,296
Last seen: 5 minutes, 47 seconds
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Re: LC stalled because all the nutes have been eaten? [Re: SWBZA]
#28631658 - 01/23/24 02:38 AM (5 days, 5 hours ago) |
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Iβve only made SSLC like 3 tickets but I literally just hand swirled it for a few minutes 2-3 times a day. Beyond that I have no useful input.
-------------------- π
π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ Help US help YOU TEK 2023 Dehydrator TEK
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SWBZA
SV-10



Registered: 05/09/23
Posts: 81
Last seen: 14 minutes, 43 seconds
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Re: LC stalled because all the nutes have been eaten? [Re: Screwup] 1
#28637578 - 01/28/24 02:37 AM (5 hours, 3 minutes ago) |
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Update
Day 22 since making the LCs.
I decided to see what happens if I stop stirring and just put the LCs in a dark cupboard and let them be. I stirred the living daylights out of both and parked them.
Jack Frost:

Inca Stargazer:

What I find interesting is that the myc did not settle to the bottom, but seems to have stayed in suspension. Weird...
Both cultures are now on agar to test their health. If they are solid, they are going into syringes and the fridge. And maybe on grain.
Good luck with all your LCs out there. I've decided 0.16% LME (1g on 600ml) filtered once, is my game until further notice. Get good enough growth, suck up into syringes, store cold. See this thread where I learned that trick:
https://www.shroomery.org/forums/showflat.php/Number/28605443/fpart/1/vc/1
Kind regards.
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