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Offlineexcrypt
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Registered: 05/30/23
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mycelium growth has stopped
    #28523687 - 10/30/23 05:11 PM (2 months, 27 days ago)

Hi Guys

I am new to this so I am hoping for some advice on how to move forward.

I have cloned a mushroom from a dehydrated sample and isolated it on an agar plate.

I have then inoculated a spawnbag of rye grain and has been sitting for 2 and a half months. This seems to be along time so something it up. I believe the grain is too dry?


To prepare the rye grain this is what I did:
Washed rye grain 3-4 times
Added some Gypsum.
Soaked for 24hrs
Boiled grains for 15min
Cooled, drained and dried grain.

I had it fanned dried so this is probably where I went wrong as I was worried about it being too wet.

Filled spawn bag and sterilized in pressure cooker.
When cooled, I opened and inoculated the spawn bag with the agar under a flowhood.

I don't think there is enough moisture. Should I start again or can this be recovered? The bag has a filter patch and is not blocked.



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InvisibleBaba Yaga
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Registered: 09/13/20
Posts: 3,955
Loc: Hyperspace Chicken Coop
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Re: mycelium growth has stopped [Re: excrypt]
    #28523850 - 10/30/23 07:42 PM (2 months, 27 days ago)

Yep, that is a long time, try giving it the good ol' shake treatment.


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OfflinePBJ710
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Registered: 07/05/19
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Re: mycelium growth has stopped [Re: Baba Yaga] * 1
    #28523854 - 10/30/23 07:49 PM (2 months, 27 days ago)

:whathesaid:

If you're sure it's just dry, you could also G2G to some more hydrated grains and it should take off if the culture is healthy.


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Offlineexcrypt
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Registered: 05/30/23
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Re: mycelium growth has stopped [Re: excrypt] * 1
    #28630577 - 01/22/24 12:36 AM (6 days, 7 hours ago)

Thanks guys,

I ended up ditching it and when I opened it up there was some contamination also. I had previously isolated it on agar anyway. So will give it another go.


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OfflineHappinessStan
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Registered: 10/10/12
Posts: 1,612
Loc: Worcester, UK
Last seen: 4 hours, 13 minutes
Re: mycelium growth has stopped [Re: excrypt] * 1
    #28630654 - 01/22/24 04:34 AM (6 days, 3 hours ago)

Quote:

excrypt said:
Thanks guys,

I ended up ditching it and when I opened it up there was some contamination also. I had previously isolated it on agar anyway. So will give it another go.



Might not hurt to post photos of your agar before you go to spawn next, that may be where the contam is coming in.
Good luck on your next grow. Props for knowing when to throw/start again.


--------------------



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