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dumbomumbo
yourdad

Registered: 01/23/23
Posts: 13
Loc: ohio
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cloning dry mushrooms
#28154757 - 01/23/23 10:40 PM (1 year, 4 days ago) |
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hi, my first post here. i want to clone my mushrooms, because its the best strain ive found so far, i got it from a seller and want to clone it. i followed RRโs advice from a post and cleaned the little 0.5mm dry bits multiple times in distilled water, rehydrated them and then put it in a agar dish, thing is, i am getting really slow growth,6 days in and a little dot in the dish. am i doing something wrong ? help me guys, i wanna make this possible!
-------------------- Just an old guy waiting to leave.
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dumbomumbo
yourdad

Registered: 01/23/23
Posts: 13
Loc: ohio
Last seen: 10 months, 6 hours
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fyi i am using barley malt and agar agar recipie
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Excess Taters
Highentist



Registered: 08/29/22
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Re: cloning dry mushrooms [Re: dumbomumbo] 2
#28154766 - 01/23/23 11:06 PM (1 year, 4 days ago) |
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All I really know about this is that it's a complete crap shoot and never guaranteed. So any growth at all is actually a sign that shit should work out, assuming it's not some other sort of growth.
Here are some links that may help, I did a rando search to see if there was a definitive easy answer, but I think research and trial + error are in your future.
https://www.shroomery.org/forums/showflat.php/Number/10831802 https://mycotopia.net/topic/19440-cloning-dried-mushrooms/
Quote:
Pastywhyte said: Probably cause the info it contained was a bad idea for most folks. I tried to clone dried fruits once upon a time. The result was disgusting Your best bet would be to use antibiotic agar and place gill fragments on the plate. Depending on how it was dried and how old it was the spores could germinate. However unless your trying to resurrect something really special (like some rare species) your best bet would be to just start over from spores At any rate the result would not be a clone, it would be ms.
-------------------- If you've never grown mushrooms before, here's how you start. First, follow the Updated PF Tek, put 4 holes in jar lids not 1, and use a water tub! The next move is Shoebox Tek. After that you move onto grains, agar, monotubs. Agar is easy, just do it. Other useful links - Picture guide for how things should look and proper surface conditions guide Growing APE or PE? P9 pseudo casing tek
Edited by Excess Taters (01/23/23 11:07 PM)
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dumbomumbo
yourdad

Registered: 01/23/23
Posts: 13
Loc: ohio
Last seen: 10 months, 6 hours
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this article got me ready to start
https://www.shroomery.org/forums/showflat.php/Number/18929216/fpart/all
i successfully did that, but after 6 days growth is very little, all i saw was a spot on the dried tissue.
should i try adding yeast to my agar mix?
-------------------- Just an old guy waiting to leave.
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hao
Last one standing

Registered: 01/23/23
Posts: 53
Last seen: 11 months, 14 days
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Good luck with that.
Never tried myself but it's a good challenge. A lot of risk of growing mold or contam instead of the original genetics
But a good challenge ๐
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Excess Taters
Highentist



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Re: cloning dry mushrooms [Re: dumbomumbo] 1
#28154841 - 01/24/23 01:00 AM (1 year, 4 days ago) |
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Quote:
dumbomumbo said: this article got me ready to start
https://www.shroomery.org/forums/showflat.php/Number/18929216/fpart/all
i successfully did that, but after 6 days growth is very little, all i saw was a spot on the dried tissue.
should i try adding yeast to my agar mix?
There's not much I can add, but if you wanted to ask this question in the trusted cultivators thread, you might get a better answer. They gots more brains and experiences than us common folks'
https://www.shroomery.org/forums/showflat.php/Number/24679216/fpart/110/vc/1
-------------------- If you've never grown mushrooms before, here's how you start. First, follow the Updated PF Tek, put 4 holes in jar lids not 1, and use a water tub! The next move is Shoebox Tek. After that you move onto grains, agar, monotubs. Agar is easy, just do it. Other useful links - Picture guide for how things should look and proper surface conditions guide Growing APE or PE? P9 pseudo casing tek
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Pickle Rick
Gherkin



Registered: 07/24/22
Posts: 864
Loc: Jar
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I tried this a couple times - ended up with contam
-------------------- P i c k l e R i c k
Pick Rick's Mushroom Chocolate
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hao
Last one standing

Registered: 01/23/23
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Well the whole process of drying the mushroom is ending on killing the cells... With no alive cell on your agar, you won't grow anything but contam...
The only way it works is if you have some survivor cell. Or some still viable spores
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reximusprime
Stranger

Registered: 11/25/23
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Re: cloning dry mushrooms [Re: dumbomumbo]
#28558340 - 11/27/23 12:51 PM (1 month, 30 days ago) |
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I am attempting to clone from dried fruits also. After reading this post and several others as well as watching some vids on the tube, I got a decent idea of the process. I had to visit several stores to find all the materials but it wasnt too bad, about 75CA for everything.

I followed the nutrient agar recipe found somewhere here. My adhd helps me research tf out of things but keeping track of sources is a nightmare. I used 10g of agar and 10ml of liquid malt per 500ml of water. Boiled it until it was dissolved and skimmed the foam off same as when making jam. I then added 2-3 tablespoons of the hot agar mix to clean 125ml jars. Filled 14 in total. I lidded and ringed the jars and put them in my instapot. They pressure cooked on high for 20min and I let them cool overnight.



Next day I took them out and dried them off. I bought two 20x20 furnace filters, one basic and one with the highest filtration on the shelf to put on my box fan. The basic one is on the back to catch the big stuff like dust and hair etc. The fine one went on the front to filter the small stuff. Lined that up with a modified box to create a juryrigged flow box. With the fan on low and both filters I could feel the air moving but it was slow enough not to disturb the process. I placed 10 jars, cutting board, scalpel, extra knife, package of dried fruit bodies, etc in the work area and methodically sanitized everything with the fan on.


Once the sanitizer dried I cracked the seal on the jar lids and then the package. In five jars I scraped random pieces of gill from the biggest cap in hopes some spores are present. In four jars I placed pieces of stem I had split open, in hopes that some inner tissue is clean and viable. The last jar has both a piece of stem and some gill because why not?

I capped and labelled all the jars and will see how it goes. They are on a shelf at about 68ยฐf. If they fail to grow I think it will be sad but a learning experience for the process. I have no idea how they were dried or how long ago. I kept 4 jars sealed as a control. If they all end up with contam then I know my process was insuficient to maintain sterility. Absolute worst case I learn something and the garden gets a bit of compost. Best case I get one or two jars with myco and I save a bit of coin from having to order genetics. The materials wont be a waste as I used a small fraction of the agar and malt syrup, everything else can be reused.
I did not presoak the dried fruit. For one it is a possible route to spread contam and two the jars had enough condensation inside for the bits I added to rehydrate.
-------------------- Do what you can, with what you have, where you are. Theodore Roosevelt
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JW123
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Registered: 01/17/22
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starvinghooker
Voyager



Registered: 04/16/23
Posts: 533
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Re: cloning dry mushrooms [Re: JW123]
#28558732 - 11/27/23 06:54 PM (1 month, 30 days ago) |
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Might trying something like this stand a better chance if a person were to use the caps, hoping for some viable spores to be in them?
-------------------- Grab life by the balls and yank on 'em til everything you want comes gushing out in thick, creamy ribbons Noob like me? Start here! Hitchhiker's Guide to the Shroomery
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,528
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Those are gonna blow up.
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reximusprime
Stranger

Registered: 11/25/23
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Re: cloning dry mushrooms [Re: JW123]
#28613055 - 01/07/24 01:58 PM (20 days, 17 hours ago) |
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I did read that post and it was very informative. And no they didn't blow up because the lids aren't on tight.
Out of 10 jars 2 were overtaken by green fuzzies.

The 4 remaining jars with just gill membrane have possible growth but also some potential contam on the gill pieces.




The 3 jars with stem material are fairly clean with possible growth and a bit of fuzz on the pieces.



The 1 jar with both stem and gill is the same.

I'd say my air handling setup works fine with 8/10 clean transfers. Not knowing the history on the dried fruit I can't be certain of all variables. I am using the same filter setup to clone lions mane and shiitake. Any advice on the condition of the growth is most welcome. I'll try cloning another batch of dried fruit when I get some in.
-------------------- Do what you can, with what you have, where you are. Theodore Roosevelt
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Screwup
Googles your dumb questions


Registered: 01/27/22
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Isโฆthis not just mold and bacteria? Am I dummy?
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๐ด ๐ฐ ๐ผ ๐ฒ ๐ป ๐ธ ๐ฝ ๐ถ ๐
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๐ฐ ๐ฟ Help US help YOU TEK 2023 Dehydrator TEK
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HelloImBob
Old Guy

Registered: 03/30/08
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Re: cloning dry mushrooms [Re: Screwup]
#28613596 - 01/08/24 03:54 AM (20 days, 3 hours ago) |
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I have to agree those jars look scary, I mean there could be a little mycelium growth in a couple but they look closer to the mold test kit.
You might get lucky and get a few spores to germinate from the gills but you might have contams on your dry material as well.
Without control experiments on your filter setup you don't know where the contams are coming from other than their location on the agar
Have you tried exposing the agar to the filtered air for a minute or less to see if it's actually clean air?
To know that nothing will grow on your control agar after exposure will help you most of all narrow down where the problems are coming from
Good luck doesn't seem worth the time unless your just doing it for experience
Otherwise you might get frustrated before you even get started on what could be a very fun hobby
I would for sure run experiments on the filter setup first though so you don't have to ask yourself all the questions that can make you want to pull your hair out when dealing with clean work and contams
-------------------- Quote from Stipe-n-Cap "You appear to be talking about boosting tryptamine content in mycelium by amending LC with....whatever your amendments are. I have to say I'm a tad disappointed that you're addressing us with the shorthand of a 13 yo girl who's texting her besty for make-up tips, instead of proper English, which causes me to have doubts."
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reximusprime
Stranger

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Re: cloning dry mushrooms [Re: HelloImBob]
#28630355 - 01/21/24 07:21 PM (6 days, 12 hours ago) |
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Thank you for the feed back, I appreciate it and take it to heart. I am just playing around and am not seriously invested in the reanimation. I have exposed a couple jars to the airflow and recapped them. I'll give them a week and then check. I'm mostly just getting a feel for all the tek and processes before I seriously commit to growing a bunch. Dont even have more than a shelf for extra space at the moment.
These are two lions mane that I cloned in the same setup.


These are the two remaining jars of the dry GT that I am attempting to reanimate. One is the stem material and the other is a sprinkling from the gills. The rest have too much obvious contam.
T

My agar mix seems to be stable and the sanitizer function on the instapot seems to be doing as well as a pressure canner. I made a few small jars of growth media, grains and coir mix, to use as a small grow test if I do indeed salvage some myco. I had read somewhere of an antibacterial agar mix to prevent bacteria based contam but cant seem to find it again.
-------------------- Do what you can, with what you have, where you are. Theodore Roosevelt
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The Holy Reality
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Registered: 02/19/22
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It's been documented on here many times. It can be done as I have done it myself with 1 1/2 year old dried GT's. My best growth on Agar came from a gill sample & by best growth I mean least amount of contam. Used pastyplates, no rehydrating, just dry sample straight to the plate. You have to stay on top of it and transfer clean mycelium ASAP to a clean plate cause the nasties come quick. Is it worth it? Doubt I would do it again. I got two good flushes from it but really just wanted to see if I could do it. Give this a read!
https://www.shroomery.org/forums/showflat.php/Number/28545857#28545857
-------------------- Don't tell & don't sell
Edited by The Holy Reality (01/21/24 10:14 PM)
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HelloImBob
Old Guy

Registered: 03/30/08
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Quote:
reximusprime said: Thank you for the feed back, I appreciate it and take it to heart. I am just playing around and am not seriously invested in the reanimation. I have exposed a couple jars to the airflow and recapped them. I'll give them a week and then check. I'm mostly just getting a feel for all the tek and processes before I seriously commit to growing a bunch. Dont even have more than a shelf for extra space at the moment.
These are two lions mane that I cloned in the same setup.


These are the two remaining jars of the dry GT that I am attempting to reanimate. One is the stem material and the other is a sprinkling from the gills. The rest have too much obvious contam.
T

My agar mix seems to be stable and the sanitizer function on the instapot seems to be doing as well as a pressure canner. I made a few small jars of growth media, grains and coir mix, to use as a small grow test if I do indeed salvage some myco. I had read somewhere of an antibacterial agar mix to prevent bacteria based contam but cant seem to find it again.
But hey those bottom 2 plates don't look that bad, I didn't think anything would come of it from the first set of pics.
Good job on experimenting it looks like you could do quite well especially with better starting materials.
They used to say sky is the limit right? We all better than that 
Don't let anything stop you from trying to achieve what you want.
-------------------- Quote from Stipe-n-Cap "You appear to be talking about boosting tryptamine content in mycelium by amending LC with....whatever your amendments are. I have to say I'm a tad disappointed that you're addressing us with the shorthand of a 13 yo girl who's texting her besty for make-up tips, instead of proper English, which causes me to have doubts."
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