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Offlineballysocket
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pe on agar
    #28629955 - 01/21/24 02:46 PM (6 days, 17 hours ago)

I have three plates that I inoculated with a pe mss. My only agar experience is cloning pins from a pf tek. Does any of this mess look viable?
Thank you in advance!





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Offlinefraki58
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Re: pe on agar [Re: ballysocket]
    #28629963 - 01/21/24 02:51 PM (6 days, 17 hours ago)

Edit: page wasnt showing me the pics

Growth looks good but man i dont know if the green stuff is all contamination or just the media.
Whats your agar recipe?


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If any of the said above is incorrect, please correct me! Still learning here and constructive criticism is the best :smile:


Edited by fraki58 (01/21/24 02:54 PM)


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Offline3.A.M
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Re: pe on agar [Re: ballysocket]
    #28629974 - 01/21/24 02:55 PM (6 days, 16 hours ago)

What’s that agar recipe dude?
It’s pretty manky but appears to be working, I’m just not sure if the growth is due to the agar or contamination but maybe the leading edge from the top left myc in the first pic,
(Edit: centre top left, not that thing in the far top left, didn’t notice that) it might have something clean in it, transfer it to a brf puck maybe, or a water agar plate to be sure though.


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Edited by 3.A.M (01/22/24 03:03 AM)


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Offlineballysocket
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Re: pe on agar [Re: 3.A.M]
    #28629982 - 01/21/24 03:01 PM (6 days, 16 hours ago)

I expected the agar questions. I ran some wbs and whole oats through the coffee maker. It looks like puke but worked well for cloning in the past.


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InvisibleChanged


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Re: pe on agar [Re: ballysocket]
    #28629988 - 01/21/24 03:07 PM (6 days, 16 hours ago)

Its really not hard to mix up some malt extract and water if you don't want your plates to look like vomit...


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Offlineballysocket
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Re: pe on agar [Re: Changed]
    #28629991 - 01/21/24 03:10 PM (6 days, 16 hours ago)

Quote:

Changed said:
Its really not hard to mix up some malt extract and water if you don't want your plates to look like vomit...



It's even easier to use what you have on hand. Also the appearance doesn't bother me. But if you had any input on which plate I should go with...


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InvisibleChanged


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Re: pe on agar [Re: ballysocket] * 2
    #28629997 - 01/21/24 03:14 PM (6 days, 16 hours ago)

Well, because your agar has a bunch of shit in it, its very difficult to tell if the growth is bacterial or mycelial.


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Offlineibz
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Re: pe on agar [Re: Changed]
    #28630005 - 01/21/24 03:19 PM (6 days, 16 hours ago)

Quote:

Changed said:
Well, because your agar has a bunch of shit in it, its very difficult to tell if the growth is bacterial or mycelial.



:whathesaid:


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OfflineAspectOfTheCreator
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Re: pe on agar [Re: ibz] * 1
    #28630011 - 01/21/24 03:26 PM (6 days, 16 hours ago)

I could never bring myself to trust those plates.


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Offlineballysocket
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Re: pe on agar [Re: AspectOfTheCreator]
    #28630067 - 01/21/24 03:57 PM (6 days, 15 hours ago)

I thought I read somewhere that grain flour was an acceptable nutrient for agar? How does one achieve clarity with grain flour?


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InvisibleChanged


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Re: pe on agar [Re: ballysocket]
    #28630134 - 01/21/24 04:33 PM (6 days, 15 hours ago)

Do you not have access to Amazon, or a local brewing store? A bag of light malt extract might run you $5.

But if you're insistent on doing things your own way, you're likely going to continue to struggle.


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OfflineBobgas
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Re: pe on agar [Re: Changed]
    #28630140 - 01/21/24 04:44 PM (6 days, 15 hours ago)

top plate tiny piece from the large 2 center blobs at 12 o'clock and 3:30 from the lower center.

That recipe is pretty rough to discern what's contam.


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🅃🄴🄰🄼  🄲🄻🄸🄽 🄶🅆🅁🄰🄿


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Offlineballysocket
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Re: pe on agar [Re: Changed]
    #28630249 - 01/21/24 05:56 PM (6 days, 13 hours ago)

Quote:

Changed said:
Do you not have access to Amazon, or a local brewing store? A bag of light malt extract might run you $5.

But if you're insistent on doing things your own way, you're likely going to continue to struggle.



Doing things my own way?
Using grain flour is referenced in the agar write up linked in the Hitchhiker's guide.
But thanks for your input


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OnlineThe Tao
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Re: pe on agar [Re: ballysocket]
    #28630365 - 01/21/24 07:31 PM (6 days, 12 hours ago)

Not everything dissolves completely, some agar recipes will be cloudy.


Edited by The Tao (01/22/24 06:29 AM)


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OfflineCocaineBuffet
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Re: pe on agar [Re: ballysocket] * 1
    #28630416 - 01/21/24 08:25 PM (6 days, 11 hours ago)

Quote:

ballysocket said:
I expected the agar questions. I ran some wbs and whole oats through the coffee maker. It looks like puke but worked well for cloning in the past.




Grain WATER is a resourceful way to make plates out of the grain run off that you used to hydrate spawn. I don't think there is a tek from the past few years that recommends blending whole grains into a powder


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OfflineHappinessStan
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Re: pe on agar [Re: ballysocket]
    #28630427 - 01/21/24 08:42 PM (6 days, 11 hours ago)

Quote:

ballysocket said:
I have three plates that I inoculated with a pe mss. My only agar experience is cloning pins from a pf tek. Does any of this mess look viable?
Thank you in advance!







I think you need to start again with better advice.
That is almost certainly cube mycelium but, it's completely shrouded by obvious contams.
How did you sterilise those agar plates?


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OfflineThe Holy Reality
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Re: pe on agar [Re: HappinessStan]
    #28630523 - 01/21/24 10:25 PM (6 days, 9 hours ago)

Make some better Agar & do what Bobgas says. Keep transferring small samples till it's clean.


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Don't tell & don't sell


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OfflineHelloImBob
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Re: pe on agar [Re: The Holy Reality]
    #28630566 - 01/22/24 12:23 AM (6 days, 7 hours ago)

Plus what does it matter to make a few new plates, a little time and effort but it will be worth it. A few drops of spores?

Your not trying to fruit on your agar and nutrition is way overboard either way... You can get amazing results with just grain soak water LC/agar.

Nobody is trying to be mean they are just speaking from experience, it's harder to tell on that kind of background what your looking at compared to like looking at jello...


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Quote from Stipe-n-Cap

"You appear to be talking about boosting tryptamine content in mycelium by amending LC with....whatever your amendments are. I have to say I'm a tad disappointed that you're addressing us with the shorthand of a 13 yo girl who's texting her besty for make-up tips, instead of proper English, which causes me to have doubts."


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Offlineballysocket
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Re: pe on agar [Re: Bobgas]
    #28630690 - 01/22/24 06:20 AM (6 days, 1 hour ago)

Quote:

Bobgas said:
top plate tiny piece from the large 2 center blobs at 12 o'clock and 3:30 from the lower center.

That recipe is pretty rough to discern what's contam.



Thank you.


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Offlineballysocket
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Re: pe on agar [Re: The Holy Reality]
    #28630691 - 01/22/24 06:20 AM (6 days, 1 hour ago)

Quote:

The Holy Reality said:
Make some better Agar & do what Bobgas says. Keep transferring small samples till it's clean.



Thank you.


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Offlineballysocket
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Re: pe on agar [Re: HelloImBob]
    #28630693 - 01/22/24 06:23 AM (6 days, 1 hour ago)

Quote:

HelloImBob said:
Plus what does it matter to make a few new plates, a little time and effort but it will be worth it. A few drops of spores?

Your not trying to fruit on your agar and nutrition is way overboard either way... You can get amazing results with just grain soak water LC/agar.

Nobody is trying to be mean they are just speaking from experience, it's harder to tell on that kind of background what your looking at compared to like looking at jello...



Thank you.
It's 8% grain flour, as prescribed in the article I read. This mix worked really well for cloning. Would food coloring work for visual identification or does it need to be translucent?


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Offlineballysocket
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Re: pe on agar [Re: HappinessStan]
    #28630700 - 01/22/24 06:28 AM (6 days, 1 hour ago)

Quote:

HappinessStan said:
Quote:

ballysocket said:
I have three plates that I inoculated with a pe mss. My only agar experience is cloning pins from a pf tek. Does any of this mess look viable?
Thank you in advance!







I think you need to start again with better advice.
That is almost certainly cube mycelium but, it's completely shrouded by obvious contams.
How did you sterilise those agar plates?



I pc'd for 45 minutes. One of the reasons I chose grain flour was to pc a bit longer without worrying about overcooking sugar. Also, I thought it would be beneficial to germinate on the food source I will be colonizing for spawn.
Maybe I just stick to cloning pins.
Thank you.


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