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Funfarm
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LC 2 sub
#28628593 - 01/20/24 01:42 PM (7 days, 18 hours ago) |
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I was going to title this thread "Albino tidal wave", but thought it might take away from the type or source giving the high percentage of failure for said experience. I could not move forward in the hobby without giving this a try. I'm now utilizing substrate from a local culinary shop that supply's restaurants with different gourmet mushroom varieties. Since the sub bags have rubber ports, I can't not try, it's been calling my name for some time now. I elected to try a vendors albino t/wave for this experiment, "LC 2 sub". Not to take away from the vendor, as stated I recognize the high probability for failure with this inoculation technique. If I get lucky, the bag does colonize, I'm going to strip the plastic from the top, then put the block in a smaller tub with fae. A chance to try a new source and technique, all too exciting for me.
Edit here: To do over I wish I would of considered a pan species, but perhaps best I start with a cube for this project? I tell you now (while I hope not to anger my go to vendor for clean rye bags), but if I get this ATW LC 2 sub to work, I'm doing a pan selection next and would consider elimination of the whole rye berry step in process for now on by growing just blocks not bulk. I never "ran a pan", lol, but understand they like manure and this sub is fairly shitty. I know one thing don't bite your fingernails after playing in it.
Edited by Funfarm (01/20/24 07:19 PM)
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MonkeyJesusFresco
am i suspended in agar?



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Re: LC 2 sub [Re: Funfarm] 1
#28629122 - 01/20/24 07:49 PM (7 days, 12 hours ago) |
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Quote:
I recognize the high probability for failure
well that's good... so are you just gonna jam some LC into a bag of sterilized substrate?
is that a common practice? what makes you feel like there is a high failure rate for this approach?
is there anything you feel like you could do different in order to fully colonize without failure?
-------------------- LAGM v 2.024 - endo cabendo
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Funfarm
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Yes, poke and push lc, then pray to Jesus it works out. The wood loving sub the shop has available is marked sterilized and the dung loving sub is marked pasteurized.
Common practice? For me or the mycology field in general? I would say safely the common practice is a healthy amount of spawn to sub ratio, rather than a couple CC's of lc to a brick of shit. High failure rate, what makes me think that? Just the common practice of what I just wrote coupled with the limited research I have done concerning the experiment.
Is there anything I could do differently to fully colonize without failure? Without spawn first, no nothing I have experienced or read. Do you have any suggestion?
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
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i dunno, if you're convinced it won't work... maybe don't waste the LC?
or at the very least waste it on a grain bag... then ya know,
spawn that to the pasteurized sub
-------------------- LAGM v 2.024 - endo cabendo
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Funfarm
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Yes, thank you for that. I want to apologize for sounding like a asshole with the following response but doing what you said kinda defeats the experiment. I'm not convinced it won't work, I said there's a high probability it won't and I want to deflect from the vendor if it doesn't, given what Im attempting is not the norm. It's not that I'm loaded with excess cash but the experiment is within budget for my mycology hobby. A lot of what I'm doing has come from experience with failure and success, basically finding out what works for me and doesn't. Agar I suck at, and have no desire, mail ordering supplies, and shooting goo into bags I'm great at. Plus the fact that someone who runs a successful culinary mushroom shop has suggested going straight to sub with culture thus the rubber port on the supplied sub bag. Again I don't want to be asshole as I appreciate ALL input or question, but I'm just experimenting, it's not a big deal if it doesn't work out. If it does it will definitely pose the possibility of change in my technique, thus the cost vs. risk is well worth it. Thank you my friend
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MonkeyJesusFresco
am i suspended in agar?



Registered: 10/09/12
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nah you good,
-------------------- LAGM v 2.024 - endo cabendo
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Funfarm
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Cool and thank you. In my narcissistic and paranoid mind, It poses a threat to grain vendors with this unacceptable process by attempting to eliminate the spawn step. If I'm successful it will forever change the dynamics of mycology along with the transfer of revenue for grain vendors, hope mine isn't watching lol. So the powers that be, will be sure to intercept my clean package and switch it with tainted cultures! Have the damn light bulb people (illuminates) infiltrated the psychedelic sub culture? Now I must use someone else's credit card to order more culture to a phantom address. Lol, did I mention I'm a paranoid narcissist, I'm being yelled at to get back in my cage now. MonkeyJesusFresco you just made me realize I should of waited to run my mouth concerning this experiment until I received all supplies. We must be in bat country.
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MonkeyJesusFresco
am i suspended in agar?



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Re: LC 2 sub [Re: Funfarm] 2
#28629256 - 01/20/24 09:43 PM (7 days, 10 hours ago) |
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Quote:
It poses a threat to grain vendors
I assure you... it does not.
-------------------- LAGM v 2.024 - endo cabendo
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Funfarm
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Ahhhhh.... serenity now. Thank you for the well vetted response. It takes both Jesus and MonkeyJesusFresco to bring me peace of mind. Thank you friend for curbing my recently racing mind, I'm still worked up over the green bay kicker blowing that playoff game. Anyways yes I'm a small fish in a very big sea and what I do makes no vibration in the ocean. Yet I like to think I do. I wish you nothing but the best, here Im worried I gave you offending responses and you bring me Peace.
See below, "they didn't let me finish". Thru you I became more educated and appreciate it immensely. I did not realize your avatar was a botched touch up of highly regarded Spanish art. Look at the woman's expression and body language it makes me want to sling horse apples at her, all the while Jesus would kiss her.

Edited by Funfarm (01/20/24 10:32 PM)
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OldManRiver
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Re: LC 2 sub [Re: Funfarm] 2
#28629286 - 01/20/24 10:41 PM (7 days, 9 hours ago) |
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"poses threat to grain vendors"
Tell me you don't understand the biology of our fungi without telling me you don't understand our fungi.
Mycelia needs calories, my friend. What most of us call substrate doesn't contain calories. Squirting LC into coir is going to be disappointing.
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Funfarm
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Here was a long winded defensive reply to OldManRiver that only served to further substantiate my ignorance. It was bugging the shit out of me. I feel much better just deleting it and being honest with myself.
Thank you for bringing me up to speed on the calories with spawn thing, I did not realize that is the science involved. Kinda like growing a plant, the spawn acts as the seed and the sub is the dirt, you can't have tomatoes without both elements involved. Or is it more the rye is the energy for the mass of mycelia and the sub is the energy for the fruiting body? That I can understand about our fungi but I'm stubborn and will do it anyway. For the record, no I don't understand the biology of the fungi but I do know how to whip out the credit card and mail order stuff. Yes, I'm a product of internet mycology and hats off to people like you who have done your homework, and hard work. As I must quote another member "we stand on the shoulders of giants".
Edited by Funfarm (01/21/24 11:04 AM)
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Funfarm
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Giving it more thought of what you said, I'm going to ask the shop keep when I pick up another bag of this sub for this experiment. Perhaps, it has a proprietary element that acts as calories in their mix?
Edited by Funfarm (01/21/24 02:02 AM)
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OldManRiver
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Re: LC 2 sub [Re: Funfarm] 1
#28629845 - 01/21/24 01:28 PM (6 days, 18 hours ago) |
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It's different than plants in some key ways. Plants get energy from light. Dirt provides them water, and some nutrients that allow them to use the light. When plants grow, they extend by cell division. As the flower or leaf gets bigger, more cells are being created.
Fungi get their energy from some kind of food. Grain is that food when we grow them. The substrate provides water. When our fungi fruit, the fruit 'grows' by expanding cells with water. The mycelia extends through the substrate to get to the available water, then pumps that water into primordial fruits to make them 'grow' for us. Our goals in the substrate is to provide water, without encouraging contamination. This means we choose substrate materials precisely because they don't contain available food that contaminants can thrive in. Hence my snarky comment, for which I apologise. 
Realizing this opened my eyes about why the popular strategies and teks are effective.
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Funfarm
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Now I'm just curious, in nature without sterile rye grains, what fuels the mycelia for wild growing cubes?
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Mycolorado
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The pies likely have enough grain/seed in them to promote fruiting.
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Funfarm
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Ah yes from the animals diet, makes perfect sense
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Funfarm
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Given the prior posters input, specifically the suggestion to try something else without wasting material by one, and the direction of fungal biology by another. I have elected to bypass the LC 2 sub experiment process. Now electing to squirt 10cc's of goo into a prepared kit of 28 ounces of another goo in attempt to further the culture amount. Eventually I wanted the experience of utilizing the tissue from a preferred fruit to try to clone with a mail order culture kit but felt this way I could get some preliminary culture expansion experience with less chance of failure. I also felt by doing this, it allows me to utilize the materials without having to start another run with grain injection as a alternate.
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OldManRiver
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I don't what is in your proposed 'clone kit', but you likely don't need a kit. All you need is an xacto knife and agar plates. In a clean environment, flame your knife, cut open the fruit, and cut out an interior bit, about the size of a grain of rice. I like to get mine from the base of the stem, but I've heard of people getting it anywhere on the fruit. Drop that on a clean plate, and wait a week.
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Funfarm
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We had such a hard time with Agar plates before (mail ordered) having swimming pool moisture accumulation and contemenation. The local mushroom culinary shop has blank plates available, where they assure me I shouldn't encounter that problem provided the room temp is right. Ironically I was at the Walmart tonight looking at the 105 quart clear tubs for a possible SAB to give this another go.
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OldManRiver
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Quote:
Funfarm said: We had such a hard time with Agar plates before (mail ordered) having swimming pool moisture accumulation and contemenation. The local mushroom culinary shop has blank plates available, where they assure me I shouldn't encounter that problem provided the room temp is right. Ironically I was at the Walmart tonight looking at the 105 quart clear tubs for a possible SAB to give this another go.
Condensation is common in plates, and isn't necessarily a flaw or problem. Store them upside down to minimize that. Evviva PDA plates from the big river company work fine.
Edited by OldManRiver (01/22/24 07:51 PM)
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