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OfflineMyconewbie209
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Registered: 04/03/23
Posts: 136
Last seen: 10 months, 25 days
Trich help, how do I prevent this?
    #28627771 - 01/19/24 08:44 PM (11 months, 22 days ago)

So iv made a total of 9 tubs so far 2 of them not making it to complete colonization and being ruined by trich the other 5 actually made it to produce fruit but then again got hit my trich
Idk what else to do, I place by coco in a bucket pour boiling water into it wait for it to cool then use it to make my bins
Also I clean my bins with alcohol twice before placing the coco in them and I use gloves and spray with alcohol up to my elbow before handling the coco and spawn I also wear a mask threw the process, any feedback help idk what else to do to try and prevent this 7 out of 9 bins not making it is just not cool

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OfflineBobgas
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Registered: 09/01/22
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Last seen: 4 months, 6 days
Re: Trich help, how do I prevent this? [Re: Myconewbie209] * 1
    #28627777 - 01/19/24 08:51 PM (11 months, 22 days ago)

it's likely coming from earlier in the process.  What are the steps you follow to make your spawn?


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Offlinemetaneuralnetwork
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Registered: 12/13/23
Posts: 70
Last seen: 10 months, 12 days
Re: Trich help, how do I prevent this? [Re: Bobgas]
    #28627825 - 01/19/24 09:27 PM (11 months, 22 days ago)

How much coco at a time are you doing in the bucket tek?

The more you're doing at a time the less effective bucket tek is at getting rid of contams. Not saying that's where it's coming in at but it's something to think about.

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InvisibleGoatrider
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Registered: 04/08/20
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Re: Trich help, how do I prevent this? [Re: metaneuralnetwork]
    #28627850 - 01/19/24 09:41 PM (11 months, 22 days ago)

Did you use different buckets
for grains and coir,
or maybe the same?

      :cookiemonster:


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OfflineHappinessStan
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Re: Trich help, how do I prevent this? [Re: Goatrider] * 1
    #28628011 - 01/20/24 12:35 AM (11 months, 21 days ago)

You got photos of the grain spawn? Bet the contam is coming from the spawn.


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OfflineLordQuackington
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Registered: 01/04/23
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Re: Trich help, how do I prevent this? [Re: HappinessStan]
    #28628218 - 01/20/24 07:40 AM (11 months, 21 days ago)

Just my two cents worth...

Since changing from cheap "weed grow" 650g blocks of coir, to the individually sealed reptile substrate bricks, my trichoderma issues have massively improved. To the point where I can pinpoint many of my failures to using the blocks designed for growing weed (other failures due to contaminated spawn)

For the small increase in cost, this has been the best change I've made to my process.

Peace ✌


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"All men dream: but not equally. Those who dream by night in the dusty recesses of their minds wake up in the day to find it was vanity, but the dreamers of the day are dangerous men, for they may act their dreams with open eyes, to make it possible" T.E. Lawrence

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OfflineMyconewbie209
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Registered: 04/03/23
Posts: 136
Last seen: 10 months, 25 days
Re: Trich help, how do I prevent this? [Re: LordQuackington]
    #28628979 - 01/20/24 06:03 PM (11 months, 21 days ago)

A little more info, I use coco that I bought from a bulk soil supplier near me, I buy it by the yard for the grow room and store it in a large tote that has a plastic inner liner then the actual nylon tote on the outside of it
So I use millet from wbs in quart jars
I use 6qts of coco and 2qts of grain per tub sometimes 2-1/2qts
I will upload pics of spawn shortly

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OfflineMyconewbie209
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Registered: 04/03/23
Posts: 136
Last seen: 10 months, 25 days
Re: Trich help, how do I prevent this? [Re: Myconewbie209]
    #28635163 - 01/26/24 02:09 AM (11 months, 15 days ago)








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Invisibleelasticaltiger
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Re: Trich help, how do I prevent this? [Re: Myconewbie209] * 2
    #28635164 - 01/26/24 02:28 AM (11 months, 15 days ago)

In the last picture, is the second jar from the left recovering from a recent shake? If not and that's just how it grew that jar is probably the primary culprit. Grains that aren't fully colonized are the perfect medium for mold.

Either way those jars don't look perfect to me. I probably would have spawned them individually.

Next time you do your bucket of coco leave some coco in the bucket and check on it in a couple weeks. You'll see it's not your coco prep.


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Offline3.A.M
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Re: Trich help, how do I prevent this? [Re: Myconewbie209]
    #28635189 - 01/26/24 03:43 AM (11 months, 15 days ago)

That 3rd jar is heavily bacterial and the 4th looks like it’s on its way, like elasticaltiger said they’re all suss, if it isn’t your cultures then I’d bet it’s your grains and/or how they’re prepped, sorry dude.


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OfflineMyconewbie209
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Registered: 04/03/23
Posts: 136
Last seen: 10 months, 25 days
Re: Trich help, how do I prevent this? [Re: 3.A.M]
    #28653578 - 02/10/24 12:00 AM (11 months, 22 hours ago)

So as far as grain prep, I buy wbs from Walmart and put them into a tote will up with water and pull out everything that floats, then wash it out till the water runs clear
Let soak for 24hrs, then wash it again till the water runs clear then remove the water, let it sit for an hour or 2 just so that it’s not super wet and whatever was is in the grain runs to the bottom and then fill my jars
I do only use a instapot so I have to do 2 jars at a time and I pc them for 2-1/2hrs
I usually let the jars sit for a week or 2 and every few days give them a good shake just incase the bottom grain is a tiny bit more saturated than the rest by the time I use them the moisture is evenly distributed, then I move them over to my sab and put agar into them
The last batch of grain I did sat around 3-1/2 weeks before I used them and none of them grew anything

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Invisiblemeta_mmxxii
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Registered: 08/03/23
Posts: 1,071
Loc: PNW
Re: Trich help, how do I prevent this? [Re: Myconewbie209]
    #28653604 - 02/10/24 12:37 AM (11 months, 21 hours ago)

Coco coir itself will not produce trich, as it has no nutritional value for mycelium and mold.
It always leads back to the health of your spawn or as Elasticaltiger stated, uncolonized grain can be a major catalyst for contamination. It is very deceiving stuff, I had a grow that had a killer first flush and when I spawned the tub it smelled oh so umami and delicious, but developed trich during the second flush. I determined my grain was not properly hydrated, so it did not get properly sterilized. Once I corrected this, my trich was gone.
The jars in your pics have a concerning amount of moisture which is an indicator of contamination.
Sometimes spawn is a tricky thing to diagnose.


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InvisibleGoatrider
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Re: Trich help, how do I prevent this? [Re: meta_mmxxii]
    #28653627 - 02/10/24 01:47 AM (11 months, 20 hours ago)

May i ask about sea level youΒ΄re living at?
Maybe you need to up pc time,
maybe you need a different grain prep.
You most likely generate more endospores than present
when soaking for 24 hours.

      :cookiemonster:


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Invisiblemeta_mmxxii
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Re: Trich help, how do I prevent this? [Re: Goatrider]
    #28654040 - 02/10/24 10:51 AM (11 months, 11 hours ago)

:whathesaid:


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InvisibleLadyBird123S


Registered: 09/27/22
Posts: 264
Re: Trich help, how do I prevent this? [Re: meta_mmxxii] * 4
    #28655600 - 02/11/24 02:41 PM (10 months, 30 days ago)

Quote:

meta_mmxxii said:
Coco coir itself will not produce trich, as it has no nutritional value for mycelium and mold.




Mold can grow on coco fiber for the same reasons it can grow on drywall. Spore exposure, cellulose, and humid conditions. Clean spawn is essential, as is using a quality coir (and or pasteurizing at least). Some coirs can contain mold, yeasts, and bacteria when bought.
Someone correct me if I’m wrong. Just saying not all coir is created equal.

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OfflineMyconewbie209
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Registered: 04/03/23
Posts: 136
Last seen: 10 months, 25 days
Re: Trich help, how do I prevent this? [Re: LadyBird123] * 2
    #28660258 - 02/15/24 12:56 AM (10 months, 26 days ago)

So I live in central California
I believe we’re at 68ft above sea level
I’m thinking maybe I need to fork out the money and get a real pc
The instapot I use only gets up to 11psi at the most I believe
Idk if that few psi difference can make all the difference

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Offlinefractadactyl
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Registered: 02/14/24
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Re: Trich help, how do I prevent this? [Re: Myconewbie209] * 3
    #28660270 - 02/15/24 01:24 AM (10 months, 26 days ago)

Yes, it absolutely makes all the difference.  I have to pc at 18psi at my elevation.
I had bacterial issues frequently when running at 15psi.


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InvisibleLadyBird123S


Registered: 09/27/22
Posts: 264
Re: Trich help, how do I prevent this? [Re: Myconewbie209] * 1
    #28661549 - 02/16/24 02:57 AM (10 months, 25 days ago)

A pressure cooker will be more reliable at reaching high enough temperatures and maintaining those temperatures internally for sterilizing.
Another possible factor… excessive isopropyl alcohol could be doing more harm than good, specifically residues coming into direct contact with your substrate and spawn by changing the ph and chemical composition. It can’t fully evaporate when water is reintroduced. Soap and hot water is a safer bet for cleaning bins.

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Offlinefractadactyl
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Registered: 02/14/24
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Re: Trich help, how do I prevent this? [Re: Myconewbie209]
    #28661629 - 02/16/24 06:02 AM (10 months, 25 days ago)

Honestly if you have a microscope I'd make a slide with a sliver from the cultures you're starting your jars with...
I'm not saying they're bad, but the myc looks a little off in the pics you took with the lid off.
Do your jars kinda have a coconut smell?


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OfflineMyconewbie209
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Registered: 04/03/23
Posts: 136
Last seen: 10 months, 25 days
Re: Trich help, how do I prevent this? [Re: fractadactyl]
    #28662652 - 02/16/24 05:19 PM (10 months, 25 days ago)

So this weekend ima go out and get myself a new pc and try and new batch of wbs and see how that turns out
Any pc you guys recommend? I needa upgrade anyways
I can only do 2 qt jars at a time in my instapot

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