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xVadisx
MountainsDogsAndFungi



Registered: 09/03/21
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Re: I’ve got a hunch.. EASY clone crossing [Re: Pnin] 3
#28600366 - 12/27/23 11:16 PM (1 month, 18 hours ago) |
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All Maketi:
-------------------- Thanks to everyone for sharing what they know . 165F for 24hrs. Better Holes Tek (Use a Step Drill Bit) Don't forget your towel: THE HITCHHIKER'S GUIDE TO THE SHROOMERY
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Nichrome
I'm a torso!


Registered: 12/17/18
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Re: I’ve got a hunch.. EASY clone crossing [Re: xVadisx]
#28600396 - 12/28/23 12:26 AM (1 month, 17 hours ago) |
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Thick!
-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
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xVadisx
MountainsDogsAndFungi



Registered: 09/03/21
Posts: 1,123
Loc: Secret World HQ
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Re: I’ve got a hunch.. EASY clone crossing [Re: Nichrome]
#28600550 - 12/28/23 07:00 AM (1 month, 10 hours ago) |
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Maketi would scare me in the bedroom. They're...not small!
-------------------- Thanks to everyone for sharing what they know . 165F for 24hrs. Better Holes Tek (Use a Step Drill Bit) Don't forget your towel: THE HITCHHIKER'S GUIDE TO THE SHROOMERY
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fahtster
Now With 33%More Faht



Registered: 06/17/06
Posts: 9,268
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Re: I’ve got a hunch.. EASY clone crossing [Re: xVadisx] 5
#28601405 - 12/28/23 07:02 PM (30 days, 22 hours ago) |
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Quote:
xVadisx said: All Maketi:

Very nice!
2nd clone looking a lot like the first. Could be the same culture cloned twice or very similar phenos.. well have to see what they look like finished up

Ms tub looking albino heavy with the phenos. Definitely going to swab the larger fruits

1st clone 2nd flush nothing to write home about but should put me near or above the 100g dry mark for the 2qt grow. Not bad for an f2 ime
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Pnin
Riz Gukgak


Registered: 07/18/23
Posts: 395
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Re: I’ve got a hunch.. EASY clone crossing [Re: fahtster]
#28601439 - 12/28/23 07:21 PM (30 days, 22 hours ago) |
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Lovely stipes on that last pic.
-------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿 >my end of 2023 grow journal<
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fahtster
Now With 33%More Faht



Registered: 06/17/06
Posts: 9,268
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Re: I’ve got a hunch.. EASY clone crossing [Re: Pnin] 8
#28606542 - 01/01/24 11:26 PM (26 days, 18 hours ago) |
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First flush finished up on a couple more f2 maketi tubs..
Heavy ms (dried down to 89g)

2nd clone tub. Definitely looking different than the other clone when mature.. I really like this one.. colors are great. All fruits were dense AF in both tubs.
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fahtster
Now With 33%More Faht



Registered: 06/17/06
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Re: I’ve got a hunch.. EASY clone crossing [Re: fahtster] 9
#28617976 - 01/11/24 06:49 PM (16 days, 22 hours ago) |
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Some second flushes of the above maketi f2 tubs
Ms (didn’t put a ton of effort into this one—heavily neglected)

Did get a cool half and half pigmented and albino spore producing cap

The 2nd clone tub did pretty well on the second flush..

Claketi f2 clone tub is starting to knot
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Pnin
Riz Gukgak


Registered: 07/18/23
Posts: 395
Last seen: 3 hours, 50 minutes
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Re: I’ve got a hunch.. EASY clone crossing [Re: fahtster]
#28618050 - 01/11/24 08:06 PM (16 days, 21 hours ago) |
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Hey Faht are those stipes solid?
-------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿 >my end of 2023 grow journal<
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LogicaL Chaos
Ascension Energy & Alien UFOs




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Re: I’ve got a hunch.. EASY clone crossing [Re: fahtster]
#28618133 - 01/11/24 09:24 PM (16 days, 20 hours ago) |
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That half/half gill phenotype is so cool!

Its crazy how simple the whole process is. Unreal!
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LewDoja
mad $cientist, ganjacologist


Registered: 09/18/09
Posts: 974
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Re: I’ve got a hunch.. EASY clone crossing [Re: Pnin]
#28618173 - 01/11/24 10:29 PM (16 days, 19 hours ago) |
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Quote:
Pnin said: Hey Faht are those stipes solid?
Quote:
fahtster said:
2nd clone tub. Definitely looking different than the other clone when mature.. I really like this one.. colors are great. All fruits were dense AF in both tubs.
I'm gonna take wild guess....that they are more SOLID than not.
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fahtster
Now With 33%More Faht



Registered: 06/17/06
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Re: I’ve got a hunch.. EASY clone crossing [Re: LewDoja] 2
#28618204 - 01/11/24 11:08 PM (16 days, 18 hours ago) |
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OctopusDisco
Quabity Assuance


Registered: 12/10/23
Posts: 52
Loc: In a bear hole
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Re: I’ve got a hunch.. EASY clone crossing [Re: fahtster] 3
#28619041 - 01/12/24 06:23 PM (15 days, 23 hours ago) |
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Thank you, Faht, for documenting your findings here! It's cool to know that it is possible to cross clones with standard equipment.
I'm naive to a lot of the technical details, but I've been thinking about the mechanism behind what's going on here. Could it be possible that the "pulverization" treatment is interfering with the standard clamp connection process during cell division?

The mechanism would be that the shear force lyses the leading dikaryotic cell and clamp branch, leaving a monokaryotic cell behind. This monokaryotic cell then begins replicating and looking for a mate. With only one strain, this doesn't really create anything new, since the new combinations of the 2 monokaryotic cells are the old combinations (putting aside the possibility that the pulverization impacts the genetic components of one nucleus to the extent that it mates with itself, like being super inbred). Two strains, however, have the opportunity to cross during the split seconds that they become 4 monokaryotic cells, resulting in new genetic combinations compared to the parents.
Again, I'm so far away from experienced in mycology that some of the experts here might get a good chuckle reading this. If that's the case, would you please point me in the direction of some good information so that I can learn? I'm real good with reading peer-reviewed studies, even if I'm not technically trained in mycology.
Thanks again, faht! This is awesome and I can't wait to try this out one day
Edited by OctopusDisco (01/12/24 06:24 PM)
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fahtster
Now With 33%More Faht



Registered: 06/17/06
Posts: 9,268
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Re: I’ve got a hunch.. EASY clone crossing [Re: OctopusDisco] 5
#28623601 - 01/16/24 02:56 PM (12 days, 2 hours ago) |
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Seems like the most likely explanation. The pulverizing is leaving micro pieces of myc with one nuclei of each var and when they recombine with compatible myc, a cross is created. Maybe 
Claketi clone looking like.. something lol
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
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Re: I’ve got a hunch.. EASY clone crossing [Re: fahtster] 1
#28623930 - 01/16/24 07:41 PM (11 days, 22 hours ago) |
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Cool!
Kinda looks like a KSSS mutant "Mario Shroom" grow I saw awhile back.
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fahtster
Now With 33%More Faht



Registered: 06/17/06
Posts: 9,268
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Re: I’ve got a hunch.. EASY clone crossing [Re: LogicaL Chaos] 4
#28625863 - 01/18/24 12:09 PM (10 days, 5 hours ago) |
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Claketi decided to show up for the flush haha
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fahtster
Now With 33%More Faht



Registered: 06/17/06
Posts: 9,268
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Re: I’ve got a hunch.. EASY clone crossing [Re: fahtster] 3
#28630014 - 01/21/24 03:27 PM (7 days, 2 hours ago) |
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The claketi turned out better than I was hoping for. Color is awesome. They’re not the most solid fruit.. a little hollow through the center but what is there is stout. Found a couple caps that were dropping but still had the veil attached so I got some nice dark and hopefully as clean as possible swabs.

Getting the chop tonight
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MojaveMyc
Fractal Farmer


Registered: 05/08/21
Posts: 656
Loc: Colorado
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Re: I’ve got a hunch.. EASY clone crossing [Re: fahtster] 1
#28630024 - 01/21/24 03:34 PM (7 days, 2 hours ago) |
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Oh man, now those are gorgeous
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normalperson
Stranger


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Re: I’ve got a hunch.. EASY clone crossing (swinger tek adjacent) [Re: fahtster] 2
#28630131 - 01/21/24 04:33 PM (7 days, 1 hour ago) |
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i didn't use a blender but i did mix two different LC's together and shook them for about a week. APE X Huautla
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