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OfflineFunfarm
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Registered: 01/24/22
Posts: 544
Loc: Fungal jungle
Last seen: 5 months, 19 days
B positive * 3
    #28623716 - 01/16/24 04:37 PM (1 year, 1 day ago)


B+ LC injection into 5 pounder, day 1
I didn't even want to do another tub, I wanted to focus on one at a time but got pressured into a second run. Meanwhile the lc arrived by post and sat in the box for 20 minutes after drop off, the registered temp out side is 4 degrees fahrenheit. When I was handed the parcel it was super cold to the touch. The syringe still had fluidity and a bubble would easily go up when flipped. The contained mycelium would move within the suspension when agitated. I didn't even bother taping off the rubber port like I typically do, not sure if even needed for "self sealing port". Im mad at myself for giving into doing another run, and ordering biological supplies when a freezing artic blast of air sits over the territory. I don't really care if this successfully grows out. There's sterility, there's passive cleaning prep, there's laziness, there's rawdoggin it, and finally there's straight up malfescense. About the only other thing I could of done was soaked it in the toilet, the dog was curious, I figured what can it hurt.
Not sure if the barrel in the bag is visible, oh yeah be positive

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OfflineSilentraindrops
mushlove student
I'm a teapot
Registered: 12/23/23
Posts: 223
Loc: pnw
Last seen: 10 months, 11 days
Re: B positive [Re: Funfarm] * 1
    #28623748 - 01/16/24 05:15 PM (1 year, 1 day ago)

Lol , did you shoot that whole Lc in :P . You could of just saved it in fridge.

The lc might have frozen, Lc and mss aren't as forgiving to freezing temps. Spore prints are safe. Ya i would of waited till this artic weather passes for most the northern world in next couple weeks.

Currently have my own Too much spawn too many tubs moment. I Recently got gifted unemployment  so I decided to do a "few grows" 17lbs of dry grain later lol..... I don't even have enough coir lol. Got 5lbs dry for 25 lbs of spawn left to go ...
You can always dry and store. I've been just giving out to people i barely know :P. Never know how you can make a casual acquaintances day lol.


--------------------

Edited by Silentraindrops (01/16/24 05:17 PM)

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InvisibleChanged


Registered: 05/07/23
Posts: 202
Loc: 38.864, -77.0573
Re: B positive [Re: Silentraindrops] * 3
    #28623766 - 01/16/24 05:27 PM (1 year, 1 day ago)

Went from raw doggin' it on the kitchen counter, to raw doggin' it on the toilet! :toilet:

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OfflineFunfarm
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Re: B positive [Re: Funfarm] * 1
    #28623771 - 01/16/24 05:31 PM (1 year, 1 day ago)

Hello Silentraindrops, yes it was all shot in, till the plunger bottumed out.  Had quit a bit of mycelium too, about 4 C's worth. I've seen people complain about the quantity in barrels but this one was mucho thick. I'm pretty sure it got frozen killed, the barrel and plunger were frigid in me bare hand.
Also in the PNW, it's freaking cold out. The only thing I hate about the winter here is we have to put the cats shit box in the house, they won't go outside. Come spring I pitch that nasty thing and they go outdoors to relieve themselves.

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OfflineFunfarm
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Posts: 544
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Re: B positive [Re: Changed]
    #28623774 - 01/16/24 05:33 PM (1 year, 1 day ago)

Quote:

Changed said:
Went from raw doggin' it on the kitchen counter, to raw doggin' it on the toilet! :toilet:



Raw doggin' it would be a step up in the world. This straight up consisted of feces germs and my hairy assistant 🐕‍🦺 lol.
Thanks for the upvote, not sure what it means? I'm almost at a 2 year membership and just added a avatar last month. Still figuring this out:cool:

Edited by Funfarm (01/16/24 05:51 PM)

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Offlinemetaneuralnetwork
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Registered: 12/13/23
Posts: 70
Last seen: 10 months, 12 days
Re: B positive [Re: Funfarm]
    #28623778 - 01/16/24 05:35 PM (1 year, 1 day ago)

it's the freezing that kills it I believe. Cold is ok so long as it isn't frozen.

Edited by metaneuralnetwork (01/16/24 05:37 PM)

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OfflineFunfarm
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Re: B positive [Re: metaneuralnetwork]
    #28623788 - 01/16/24 05:44 PM (1 year, 1 day ago)

This is from the same vendor that got my hands on a my current run lc (PE). That stuff is going great and it didn't have half the amount of suspended mycelium. This one was pretty fat and have no doubt if it wouldn't of gotten deep freezed it would of been explosive, who knows maybe something will come of it, b positive. Disregard, someone edited previous

Silentraindrops, I forgot to add, bravo for comping folks. Your good gesture will come around to you.  I want to give away shroomy chocolates and lc in the future.

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OfflineAndImTheHighOne
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Registered: 12/06/23
Posts: 64
Last seen: 2 months, 7 days
Re: B positive [Re: Changed]
    #28623972 - 01/16/24 08:05 PM (1 year, 1 day ago)

Quote:

Changed said:
Went from raw doggin' it on the kitchen counter, to raw doggin' it on the toilet! :toilet:




Just when I thought it couldn't get any better! :lmafo:

Edit: not trying to rag on you funfarm. I think your techniques are wild, but I really do hope to see you succeed.


--------------------
"[Y]ou've already bludgeoned and abused the mycelium so at least do it justice and thoroughly mix it into the substrate :lol:" ~ Bigfoot

My LAGM 2.024

Edited by AndImTheHighOne (01/16/24 08:08 PM)

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OfflineFunfarm
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Re: B positive [Re: AndImTheHighOne]
    #28624012 - 01/16/24 08:37 PM (1 year, 1 day ago)

Hello AndImTheHighOne, why thank you much. The following is not directed at you, just pointing something in general out.
  I feel guilty as to how the mycology game has changed drastically over time. For example just the use of the internet for reference and learning, the ability to order prepped grains or substrate, down to ordering spores or lc via e-commerce. How about those old timers, the saltier growers, people registered on this very site for over 20 years. When spores had to be acquired thru the back of a high times magazine, you had to clip out a add and send in cash. People where forced to make their own spawn and sub, if you had questions... Forget about it, you had to go to the public library and check out a book. I have taken advantage of the technology, what it offers, and was not ever made to learn, bypassing the learning curve and hard work required to succeed. It's a sham that someone with no experience (like me) with a debit/credit card, internet access, and literally only a few dollars in Walmart supplies can successfully grow shrooms. It's not at all what it was like for cultivators of yesteryear, like it is today. I point the finger at myself, I'm the guiltiest. To be honest, it's a slap in the face of any old time grower, with what I do. I mean hell I push goo into a bag, modify a storage tote, spread horse shit in that tub, and whammo shrooms. I do 5% of the work a shroomer did 20 years ago and I think it goes unnoticed. If anything, I get mycelium out of this frozen B+ it only serves as a testament to how far we have come. Again not directed at you my friend, thank you
Edit, thank you for upvote

Edited by Funfarm (01/16/24 09:32 PM)

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OfflineFunfarm
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Posts: 544
Loc: Fungal jungle
Last seen: 5 months, 19 days
Re: B positive [Re: metaneuralnetwork]
    #28628624 - 01/20/24 02:31 PM (11 months, 21 days ago)

Quote:

metaneuralnetwork said:
it's the freezing that kills it I believe. Cold is ok so long as it isn't frozen.



I 🤔 think your right and Im surprised. It appears at day 4 from the shoot in there's early signs of white fuzz beginning to show face from rye kernels focused in one spot on the back of the bag. Maybe premature happiness but I'll be back with the Cyberdyne systems model 101 for sure updates of the rawdog toliet bowl inoculation. Pretty pleased for a person who didn't want to run 2 tubs at a time. This B+ specimen is tuff because it came in from a super cold box at 4° f.

Edited by Funfarm (01/20/24 03:28 PM)

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InvisibleChanged


Registered: 05/07/23
Posts: 202
Loc: 38.864, -77.0573
Re: B positive [Re: Funfarm]
    #28628833 - 01/20/24 04:43 PM (11 months, 21 days ago)

Quote:

Funfarm said:
Hello AndImTheHighOne, why thank you much. The following is not directed at you, just pointing something in general out.
  I feel guilty as to how the mycology game has changed drastically over time. For example just the use of the internet for reference and learning, the ability to order prepped grains or substrate, down to ordering spores or lc via e-commerce. How about those old timers, the saltier growers, people registered on this very site for over 20 years. When spores had to be acquired thru the back of a high times magazine, you had to clip out a add and send in cash. People where forced to make their own spawn and sub, if you had questions... Forget about it, you had to go to the public library and check out a book. I have taken advantage of the technology, what it offers, and was not ever made to learn, bypassing the learning curve and hard work required to succeed. It's a sham that someone with no experience (like me) with a debit/credit card, internet access, and literally only a few dollars in Walmart supplies can successfully grow shrooms. It's not at all what it was like for cultivators of yesteryear, like it is today. I point the finger at myself, I'm the guiltiest. To be honest, it's a slap in the face of any old time grower, with what I do. I mean hell I push goo into a bag, modify a storage tote, spread horse shit in that tub, and whammo shrooms. I do 5% of the work a shroomer did 20 years ago and I think it goes unnoticed. If anything, I get mycelium out of this frozen B+ it only serves as a testament to how far we have come. Again not directed at you my friend, thank you
Edit, thank you for upvote




We stand on the shoulders of giants...

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OfflineFunfarm
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Re: B positive [Re: Changed]
    #28628844 - 01/20/24 04:48 PM (11 months, 21 days ago)

Jolly Green Giants, not green trych ones, just Jolly Green 💚

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OfflineFunfarm
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Re: B positive [Re: Funfarm] * 2
    #28629388 - 01/21/24 02:59 AM (11 months, 20 days ago)

Yep it's definitely mycelium @ day 5. She blinded me with science.
It's poetry in motion
She turned her tender eyes to me
As deep as any ocean
As sweet as any harmony
But she blinded me with science
She blinded me with science!
And failed me in biology, hey

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OfflineFunfarm
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Re: B positive [Re: Funfarm]
    #28631185 - 01/22/24 03:21 PM (11 months, 19 days ago)

I've decided to get another single pound bag of rye to add to the existing 5 pounder. It looks like there will enough room for the whole amount in one bag without encroaching the fae patch. With a total of 6 pounds rye mixed with 20 pounds of substrate, it should get me close to a 5 inch thick cake. Of course this ratio presents a 1:3.3 ratio by weight. Its been a goal of mine for some time now to achieve a 5" tall shit cake.

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OfflineFunfarm
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Re: B positive [Re: Funfarm]
    #28635379 - 01/26/24 08:25 AM (11 months, 15 days ago)


Day 10 from LC inoculation, new bag sealer should be here today via the Amazonians. Will combine the two separate bags. Again the #'s, 6 to 20 for 5".


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OfflineFunfarm
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Re: B positive [Re: Funfarm]
    #28635803 - 01/26/24 04:07 PM (11 months, 15 days ago)



As soon as the brown truck guy dropped of the new sealer, I got to work.
  First there were 2 and then there was 1. I was worried about not having the interior bag space to shake and break up. With rolling action and vigorous bouncing I think we got it mixed up decently. Just as I was dropping the contents of the 1 lb into the bigger bag.... Like clockwork or being summoned by the contamination monster at the perfect time, here comes grandma walking thru the kitchen with a dirty dander filled king size bed comforter. Passing 1 foot from the kitchen counter with open sterile rye bags, I swear I think she did it on purpose. She's at her kung fu gym right now and I feel like throwing a dog turd in the washer with her dang comforter. If this young B+ colony survived another environmental pitfall, I'll be all to excited to see how it fruits, while right now I'm more concerned if it will come back without bacterial growth.
  I spoke with the rye vendor today and they are making up to 10 pounders now in oversize bags! Right on, next up the 10'er, and it's got to be a white surf board run (albino tidal wave).

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OfflineFunfarm
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Re: B positive [Re: Funfarm]
    #28651445 - 02/08/24 08:38 AM (11 months, 2 days ago)

Well well well.... What do we have here?  Serratia bacteria! I think I may have shot myself in the foot by trying to be cute and fancy. The combined 6 pound of rye colonized a good 50% or more and then stalled this last week. I went to break it up and the top portion of the bag that was uncolonized broke up as orange berrys. I'm thinking the exposure and transfer of sterilized rye in open air combined with a very small empty space within the combined bag (which is very little as the rye content comes right up to the fae patch) made for contamination. Oh well, a healthy LC sample and 6 pounds of rye down the drain, both items came at a discount, so just out time really. I contacted the rye vendor prior and they have up to 10 pounds of sterile berries in oversize poly bags. That's what I'll have to do next time. My apology this thread is now dead, I'll have to make it up with a B+ in the future in the correct size bag. I have shared in other places that one of my goals is a 5" tall cake in a 66 qt tub, and I thought it was so damn important. With other recent experience I'm finding a cake can prosper when finished as thin as 2". I'm kicking myself because if I would of just let the culture do it's thing inside the original 5 pounder, I would have a bulk cake baking right now. I broke the contaminated bag up real good and put it in a separate area of the rest of the projects. Maybe it will recolonize but I'm not holding out much hope with the amount of orange I witnessed. If I had to guess the volume by area equaled a half pound of pure orange berries to 5.5 pounds of healthy grain. To much moisture in that small of a space, couldn't breath and temps have been constant.

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OfflineFunfarm
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Re: B positive [Re: Funfarm]
    #28652240 - 02/08/24 08:52 PM (11 months, 2 days ago)

Orange bread mold, cause is heat and moisture. Too small of a poly fill bag to hold 6 pounds. There's not enough room to even shake up and shift the berries it's too tight. It's going to the rubbish can.

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OfflineMrJong
Autismotronic 3000


Registered: 09/30/23
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Re: B positive [Re: Funfarm] * 1
    #28652252 - 02/08/24 09:02 PM (11 months, 2 days ago)

I believe you may have accidently or not skipped a step in your sterile procedure, or the orange mold came in with the syringe (happens sometimes). Or the grains weren't sterilized properly for whatever reason. It can't show up if it's not there (or dead) in the first place if you catch my drift.

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OfflineFunfarm
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Re: B positive [Re: MrJong]
    #28652294 - 02/08/24 09:56 PM (11 months, 2 days ago)

The original 5 pounds are clean, the culture was growing in it really well (nice and white, with normal grain color), that vendor has sent me over 20 bags with no problem yet. The one pound I added to it was from a different vendor, never used before. It was transfered from bag to bag on a clean kitchen counter in open air and then heat sealed. The culture vendor is solid, haven't used a great number of their syringes but never a problem.
  Here's the timeline: It was growing really well for 10 days (immeadiatly after inoculation) healthy white no colored grains, then I combined the bags at day 11, (exactly 2 weeks ago), and then it quit growing last week. The myc growth pattern in the combined bag went up and stalled exactly where the orange clump I broke up this morning was, which is at the top of the bag. That is where a majority of the new grains sat. Suspect new grains? Hmmm?
Thank you for the input, got me thinking, the new grains might have been sterilized a long time ago and sat on a shelf, not sure may ask new vendor tomorrow.

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